Restriction map for
pF1KB5616
(Circular) (Six-base) MAPSORT of: pF1KB5616.seq Check: 3714 from: 1 to: 5477
pF1KB5616 5477 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 492 742 1312 1891 2002 492
Size: 250 570 579 111 3967
Fragments arranged by size:
3967 579 570 250 111
AccI GT'mk_AC
Cuts at: 2440 2515 2440
Size: 75 5402
Acc65I G'GTAC_C
Cuts at: 589 2493 589
Size: 1904 3573
AclI AA'CG_TT
Cuts at: 5317 5317
Size: 5477
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 340 1295 1359 3302 3734 4481 4749 325
Size: 15 955 64 1943 432 747 268 1053
Fragments arranged by size:
1943 1053 955 747 432 268 64 15
AflII C'TTAA_G
Cuts at: 2119 2119
Size: 5477
AflIII A'CryG_T
Cuts at: 677 759 1870 2829 3933 4910 677
Size: 82 1111 959 1104 977 1244
Fragments arranged by size:
1244 1111 1104 977 959 82
AgeI A'CCGG_T
Cuts at: 3865 3865
Size: 5477
AleI CACnn'nnGTG
Cuts at: 1715 1715
Size: 5477
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1918 1918
Size: 5477
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1224 1256 1224
Size: 32 5445
AlwNI CAG_nnn'CTG
Cuts at: 234 585 1117 2782 4349 234
Size: 351 532 1665 1567 1362
Fragments arranged by size:
1665 1567 1362 532 351
ApaI G_GGCC'C
Cuts at: 191 2257 191
Size: 2066 3411
ApaLI G'TGCA_C
Cuts at: 4247 4247
Size: 5477
ApoI r'AATT_y
Cuts at: 480 2362 2371 2481 4830 5086 480
Size: 1882 9 110 2349 256 871
Fragments arranged by size:
2349 1882 871 256 110 9
AseI AT'TA_AT
Cuts at: 20 20
Size: 5477
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
AvaI C'yCGr_G
Cuts at: 368 1649 2497 368
Size: 1281 848 3348
Fragments arranged by size:
3348 1281 848
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 183 216 573 606 183
Size: 33 357 33 5054
Fragments arranged by size:
5054 357 33 33
BamHI G'GATC_C
Cuts at: 1588 2502 1588
Size: 914 4563
BanI G'GyrC_C
Cuts at: 589 2493 2907 3155 3190 589
Size: 1904 414 248 35 2876
Fragments arranged by size:
2876 1904 414 248 35
BanII G_rGCy'C
Cuts at: 191 220 247 289 406 1157 1963 2058 2257
Size: 29 27 42 117 751 806 95 199
Cuts at: 2257 2491 3521 191
Size: 234 1030 2147
Fragments arranged by size:
2147 1030 806 751 234 199 117 95
42 29 27
BbeI G_GCGC'C
Cuts at: 2911 3159 2911
Size: 248 5229
BbsI GAAGACnn'nnnn_
Cuts at: 733 733
Size: 5477
BbvCI CC'TCA_GC
Cuts at: 391 391
Size: 5477
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5443
BciVI GTATCCnnnnn_n'
Cuts at: 1960 3375 4136 5477 1960
Size: 1415 761 1341 1960
Fragments arranged by size:
1960 1415 1341 761
BclI T'GATC_A
Cuts at: 2997 2997
Size: 5477
BfrBI ATG'CAT
Cuts at: 910 910
Size: 5477
BglI GCCn_nnn'nGGC
Cuts at: 1498 1581 1679 1498
Size: 83 98 5296
Fragments arranged by size:
5296 98 83
BglII A'GATC_T
Cuts at: 2992 5029 2992
Size: 2037 3440
BlpI GC'TnA_GC
Cuts at: 2589 2589
Size: 5477
Bme1580I G_kGCm'C
Cuts at: 191 336 1990 2257 2470 3102 3195 4251 191
Size: 145 1654 267 213 632 93 1056 1417
Fragments arranged by size:
1654 1417 1056 632 267 213 145 93
BmgBI CAC'GTC
Cuts at: 2438 2438
Size: 5477
BmrI ACTGGGnnnn_n'
Cuts at: 349 1587 2689 2770 3105 5249 349
Size: 1238 1102 81 335 2144 577
Fragments arranged by size:
2144 1238 1102 577 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1809 3844 1809
Size: 2035 3442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 428 918 1192 428
Size: 490 274 4713
Fragments arranged by size:
4713 490 274
Bpu10I CC'TnA_GC
Cuts at: 391 1794 391
Size: 1403 4074
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 413 1318 1336 2653 3532 4024 4322 413
Size: 905 18 1317 879 492 298 1568
Fragments arranged by size:
1568 1317 905 879 492 298 18
BsaI GGTCTCn'nnnn_
Cuts at: 5181 5181
Size: 5477
BsaAI yAC'GTr
Cuts at: 762 2459 2830 762
Size: 1697 371 3409
Fragments arranged by size:
3409 1697 371
BsaBI GATnn'nnATC
Cuts at: 6 2991 5028 6
Size: 2985 2037 455
Fragments arranged by size:
2985 2037 455
BsaHI Gr'CG_yC
Cuts at: 2908 3156 3858 5354 2908
Size: 248 702 1496 3031
Fragments arranged by size:
3031 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2666 3187 3865 4139 4286 4697 2666
Size: 521 678 274 147 411 3446
Fragments arranged by size:
3446 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 443 473 1018 1048 1352 1382 1747 1777 443
Size: 30 545 30 304 30 365 30 4143
Fragments arranged by size:
4143 545 365 304 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1624 2326 1624
Size: 702 4775
BseYI C'CCAG_C
Cuts at: 156 1584 1628 2902 3660 4237 4733 156
Size: 1428 44 1274 758 577 496 900
Fragments arranged by size:
1428 1274 900 758 577 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1830 1929 1992 1830
Size: 99 63 5315
Fragments arranged by size:
5315 99 63
BsiEI CG_ry'CG
Cuts at: 104 121 133 1917 2355 3019 3065 4273 104
Size: 17 12 1784 438 664 46 1208 1308
Fragments arranged by size:
1784 1308 1208 664 438 46 17 12
BsiHKAI G_wGCw'C
Cuts at: 220 265 1157 1606 1795 2491 3269 3459 4251
Size: 45 892 449 189 696 778 190 792
Cuts at: 4251 220
Size: 1446
Fragments arranged by size:
1446 892 792 778 696 449 190 189
45
BsmI GAATG_Cn'
Cuts at: 1645 1645
Size: 5477
Bsp1286I G_dGCh'C
Cuts at: 191 220 247 265 289 336 406 1157 1606
Size: 29 27 18 24 47 70 751 449
Cuts at: 1606 1795 1963 1990 2058 2257 2470 2491 3102
Size: 189 168 27 68 199 213 21 611
Cuts at: 3102 3195 3269 3459 3521 4251 191
Size: 93 74 190 62 730 1417
Fragments arranged by size:
1417 751 730 611 449 213 199 190
189 168 93 74 70 68 62 47
29 27 27 24 21 18
BspEI T'CCGG_A
Cuts at: 4697 4697
Size: 5477
BspHI T'CATG_A
Cuts at: 4653 4653
Size: 5477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 492 638 742 1312 1891 2002 2083 2527 2741
Size: 146 104 570 579 111 81 444 214
Cuts at: 2741 3043 3424 492
Size: 302 381 2545
Fragments arranged by size:
2545 579 570 444 381 302 214 146
111 104 81
BsrBI CCG'CTC
Cuts at: 50 135 3769 3823 5307 5472 50
Size: 85 3634 54 1484 165 55
Fragments arranged by size:
3634 1484 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 1206 1545 1902 3389 1206
Size: 339 357 1487 3294
Fragments arranged by size:
3294 1487 357 339
BsrFI r'CCGG_y
Cuts at: 1846 3475 3656 3865 1846
Size: 1629 181 209 3458
Fragments arranged by size:
3458 1629 209 181
BssSI C'ACGA_G
Cuts at: 3748 4106 3748
Size: 358 5119
BstAPI GCAn_nnn'nTGC
Cuts at: 1745 2854 1745
Size: 1109 4368
BstBI TT'CG_AA
Cuts at: 475 3838 475
Size: 3363 2114
BstEII G'GTnAC_C
Cuts at: 783 1470 783
Size: 687 4790
BstXI CCAn_nnnn'nTGG
Cuts at: 1044 1044
Size: 5477
BstYI r'GATC_y
Cuts at: 1 1588 2502 2969 2992 3327 3573 4585 4671
Size: 1587 914 467 23 335 246 1012 86
Cuts at: 4671 4683 5029 1
Size: 12 346 449
Fragments arranged by size:
1587 1012 914 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 121 1043 1187 1439 1800 1917 121
Size: 922 144 252 361 117 3681
Fragments arranged by size:
3681 922 361 252 144 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1507 3427 3608 3771 1507
Size: 1920 181 163 3213
Fragments arranged by size:
3213 1920 181 163
BtsI GCAGTG_nn'
Cuts at: 235 1006 1118 1722 2147 2720 235
Size: 771 112 604 425 573 2992
Fragments arranged by size:
2992 771 604 573 425 112
DraI TTT'AAA
Cuts at: 2476 2476
Size: 5477
DrdI GACnn_nn'nnGTC
Cuts at: 2176 3183 4041 2176
Size: 1007 858 3612
Fragments arranged by size:
3612 1007 858
EaeI y'GGCC_r
Cuts at: 118 130 463 599 1803 1914 3062 3236 3627
Size: 12 333 136 1204 111 1148 174 391
Cuts at: 3627 3654 4999 118
Size: 27 1345 596
Fragments arranged by size:
1345 1204 1148 596 391 333 174 136
111 27 12
EagI C'GGCC_G
Cuts at: 118 130 1914 3062 118
Size: 12 1784 1148 2533
Fragments arranged by size:
2533 1784 1148 12
EarI CTCTTCn'nnn_
Cuts at: 1271 2228 3500 3710 1271
Size: 957 1272 210 3038
Fragments arranged by size:
3038 1272 957 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3685 3995 4141 5287 3685
Size: 310 146 1146 3875
Fragments arranged by size:
3875 1146 310 146
EcoICRI GAG'CTC
Cuts at: 218 1155 2489 218
Size: 937 1334 3206
Fragments arranged by size:
3206 1334 937
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 340 428 918 1192 1295 1359 3302 3734
Size: 15 88 490 274 103 64 1943 432
Cuts at: 3734 4481 4749 325
Size: 747 268 1053
Fragments arranged by size:
1943 1053 747 490 432 274 268 103
88 64 15
EcoO109I rG'GnC_Cy
Cuts at: 187 273 386 699 1544 2253 2254 2616 187
Size: 86 113 313 845 709 1 362 3048
Fragments arranged by size:
3048 845 709 362 313 113 86 1
EcoRI G'AATT_C
Cuts at: 2362 2481 2362
Size: 119 5358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3496 3496
Size: 5477
FspI TGC'GCA
Cuts at: 3258 3258
Size: 5477
HaeII r_GCGC'y
Cuts at: 157 166 2911 3159 4181 5160 157
Size: 9 2745 248 1022 979 474
Fragments arranged by size:
2745 1022 979 474 248 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 1039 1066 1071 1098 1747 1779 2089
Size: 32 619 27 5 27 649 32 310
Cuts at: 2089 2121 2422 2454 3319 3349 3351 3381 388
Size: 32 301 32 865 30 2 30 2484
Fragments arranged by size:
2484 865 649 619 310 301 32 32
32 32 30 30 27 27 5 2
HincII GTy'rAC
Cuts at: 2516 2516
Size: 5477
KasI G'GCGC_C
Cuts at: 2907 3155 2907
Size: 248 5229
KpnI G_GTAC'C
Cuts at: 593 2497 593
Size: 1904 3573
MluI A'CGCG_T
Cuts at: 1870 4910 1870
Size: 3040 2437
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1862 4148 4332 1862
Size: 2286 184 3007
Fragments arranged by size:
3007 2286 184
MscI TGG'CCA
Cuts at: 601 1805 3238 601
Size: 1204 1433 2840
Fragments arranged by size:
2840 1433 1204
MslI CAynn'nnrTG
Cuts at: 493 607 1042 1715 1885 2266 3466 3593 493
Size: 114 435 673 170 381 1200 127 2377
Fragments arranged by size:
2377 1200 673 435 381 170 127 114
MspA1I CmG'CkG
Cuts at: 123 1677 1814 1913 1919 1940 2588 2902 3262
Size: 1554 137 99 6 21 648 314 360
Cuts at: 3262 4275 4520 123
Size: 1013 245 1080
Fragments arranged by size:
1554 1080 1013 648 360 314 245 137
99 21 6
NaeI GCC'GGC
Cuts at: 3658 3658
Size: 5477
NarI GG'CG_CC
Cuts at: 2908 3156 2908
Size: 248 5229
NcoI C'CATG_G
Cuts at: 1043 1187 1800 1043
Size: 144 613 4720
Fragments arranged by size:
4720 613 144
NgoMIV G'CCGG_C
Cuts at: 3656 3656
Size: 5477
NotI GC'GGCC_GC
Cuts at: 118 1914 118
Size: 1796 3681
NsiI A_TGCA'T
Cuts at: 912 912
Size: 5477
NspI r_CATG'y
Cuts at: 681 1890 2530 3030 3937 681
Size: 1209 640 500 907 2221
Fragments arranged by size:
2221 1209 907 640 500
PasI CC'CwG_GG
Cuts at: 1162 1162
Size: 5477
PciI A'CATG_T
Cuts at: 677 3933 677
Size: 3256 2221
PflMI CCAn_nnn'nTGG
Cuts at: 585 847 921 585
Size: 262 74 5141
Fragments arranged by size:
5141 262 74
PmeI GTTT'AAAC
Cuts at: 2476 2476
Size: 5477
PmlI CAC'GTG
Cuts at: 762 762
Size: 5477
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4616 4648 4616
Size: 32 5445
PpuMI rG'GwC_Cy
Cuts at: 1544 1544
Size: 5477
PspOMI G'GGCC_C
Cuts at: 187 2253 187
Size: 2066 3411
PstI C_TGCA'G
Cuts at: 1672 2524 3209 1672
Size: 852 685 3940
Fragments arranged by size:
3940 852 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5477
PvuII CAG'CTG
Cuts at: 1814 1940 2902 3262 1814
Size: 126 962 360 4029
Fragments arranged by size:
4029 962 360 126
RsrII CG'GwC_CG
Cuts at: 3672 3672
Size: 5477
SacI G_AGCT'C
Cuts at: 220 1157 2491 220
Size: 937 1334 3206
Fragments arranged by size:
3206 1334 937
SacII CC_GC'GG
Cuts at: 124 1920 124
Size: 1796 3681
SalI G'TCGA_C
Cuts at: 2514 2514
Size: 5477
SapI GCTCTTCn'nnn_
Cuts at: 2228 3500 3710 2228
Size: 1272 210 3995
Fragments arranged by size:
3995 1272 210
SbfI CC_TGCA'GG
Cuts at: 2524 2524
Size: 5477
ScaI AGT'ACT
Cuts at: 374 1744 2191 4994 374
Size: 1370 447 2803 857
Fragments arranged by size:
2803 1370 857 447
SexAI A'CCwGG_T
Cuts at: 779 779
Size: 5477
SfcI C'TryA_G
Cuts at: 33 872 1146 1668 2520 3205 4198 4389 33
Size: 839 274 522 852 685 993 191 1121
Fragments arranged by size:
1121 993 852 839 685 522 274 191
SfoI GGC'GCC
Cuts at: 2909 3157 2909
Size: 248 5229
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5477
SmaI CCC'GGG
Cuts at: 1651 2499 1651
Size: 848 4629
SmlI C'TyrA_G
Cuts at: 428 1333 1351 2119 2632 3547 4039 4301 428
Size: 905 18 768 513 915 492 262 1604
Fragments arranged by size:
1604 915 905 768 513 492 262 18
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5477
SphI G_CATG'C
Cuts at: 2530 3030 2530
Size: 500 4977
StyI C'CwwG_G
Cuts at: 1043 1187 1388 1800 2249 2611 2965 1043
Size: 144 201 412 449 362 354 3555
Fragments arranged by size:
3555 449 412 362 354 201 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 619 2369 3060 3193 3652 3861 619
Size: 1750 691 133 459 209 2235
Fragments arranged by size:
2235 1750 691 459 209 133
TatI w'GTAC_w
Cuts at: 372 1742 2189 4992 372
Size: 1370 447 2803 857
Fragments arranged by size:
2803 1370 857 447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 443 475 553 585 1352 1384 1813 1845 4994
Size: 32 78 32 767 32 429 32 3149
Cuts at: 4994 5026 443
Size: 32 894
Fragments arranged by size:
3149 894 767 429 78 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 2173 3274 2173
Size: 1101 4376
XbaI T'CTAG_A
Cuts at: 2508 2508
Size: 5477
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1437 1813 2223 2972 1437
Size: 376 410 749 3942
Fragments arranged by size:
3942 749 410 376
XmaI C'CCGG_G
Cuts at: 1649 2497 1649
Size: 848 4629
XmnI GAAnn'nnTTC
Cuts at: 481 481
Size: 5477
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AscI AvrII BmtI BsiWI BsmBI
BsrGI BssHII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NruI PacI PfoI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI