Restriction map for
pF1KB5628
(Circular) (Six-base) MAPSORT of: pF1KB5628.seq Check: 9874 from: 1 to: 7241
pF1KB5628 7241 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AatII G_ACGT'C
Cuts at: 2489 2489
Size: 7241
AccI GT'mk_AC
Cuts at: 4279 4279
Size: 7241
Acc65I G'GTAC_C
Cuts at: 4222 4257 4222
Size: 35 7206
AclI AA'CG_TT
Cuts at: 7081 7081
Size: 7241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 416 487 993 2167 2596 5066 5498 5851 6513
Size: 71 506 1174 429 2470 432 353 662
Cuts at: 6513 416
Size: 1144
Fragments arranged by size:
2470 1174 1144 662 506 432 429 353
71
AfeI AGC'GCT
Cuts at: 1714 1714
Size: 7241
AflIII A'CryG_T
Cuts at: 1121 2510 4593 6393 6674 1121
Size: 1389 2083 1800 281 1688
Fragments arranged by size:
2083 1800 1688 1389 281
AgeI A'CCGG_T
Cuts at: 3236 3236
Size: 7241
AhdI GACnn_n'nnGTC
Cuts at: 3069 3918 3069
Size: 849 6392
AleI CACnn'nnGTG
Cuts at: 944 2693 3280 944
Size: 1749 587 4905
Fragments arranged by size:
4905 1749 587
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4189 4189
Size: 7241
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3782 3814 3782
Size: 32 7209
AlwNI CAG_nnn'CTG
Cuts at: 1592 2529 3814 4546 5984 1592
Size: 937 1285 732 1438 2849
Fragments arranged by size:
2849 1438 1285 937 732
ApaI G_GGCC'C
Cuts at: 1314 1532 2406 4001 1314
Size: 218 874 1595 4554
Fragments arranged by size:
4554 1595 874 218
ApaLI G'TGCA_C
Cuts at: 1194 2624 6079 1194
Size: 1430 3455 2356
Fragments arranged by size:
3455 2356 1430
ApoI r'AATT_y
Cuts at: 542 1228 1996 2314 3489 4245 6594 6850 542
Size: 686 768 318 1175 756 2349 256 933
Fragments arranged by size:
2349 1175 933 768 756 686 318 256
AseI AT'TA_AT
Cuts at: 20 355 3653 20
Size: 335 3298 3608
Fragments arranged by size:
3608 3298 335
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7241
AvaI C'yCGr_G
Cuts at: 185 567 1446 2405 2971 3930 3976 4261 185
Size: 382 879 959 566 959 46 285 3165
Fragments arranged by size:
3165 959 959 879 566 382 285 46
AvrII C'CTAG_G
Cuts at: 1611 1611
Size: 7241
BamHI G'GATC_C
Cuts at: 3420 3588 4266 3420
Size: 168 678 6395
Fragments arranged by size:
6395 678 168
BanI G'GyrC_C
Cuts at: 225 887 1099 1498 1635 1681 2137 2213 2967
Size: 662 212 399 137 46 456 76 754
Cuts at: 2967 3370 4042 4222 4257 4671 4919 4954 225
Size: 403 672 180 35 414 248 35 2512
Fragments arranged by size:
2512 754 672 662 456 414 403 399
248 212 180 137 76 46 35 35
BanII G_rGCy'C
Cuts at: 262 596 841 1314 1447 1532 1558 1660 1993
Size: 334 245 473 133 85 26 102 333
Cuts at: 1993 2406 2917 3359 3937 4001 4255 5285 262
Size: 413 511 442 578 64 254 1030 2218
Fragments arranged by size:
2218 1030 578 511 473 442 413 334
333 254 245 133 102 85 64 26
BbeI G_GCGC'C
Cuts at: 891 1103 2141 2217 3374 4675 4923 891
Size: 212 1038 76 1157 1301 248 3209
Fragments arranged by size:
3209 1301 1157 1038 248 212 76
BbsI GAAGACnn'nnnn_
Cuts at: 3749 3749
Size: 7241
BciVI GTATCCnnnnn_n'
Cuts at: 5139 6195 7241 5139
Size: 1056 1046 5139
Fragments arranged by size:
5139 1056 1046
BclI T'GATC_A
Cuts at: 4761 4761
Size: 7241
BfrBI ATG'CAT
Cuts at: 2600 2600
Size: 7241
BglI GCCn_nnn'nGGC
Cuts at: 1466 2242 1466
Size: 776 6465
BglII A'GATC_T
Cuts at: 2534 4052 4756 6793 2534
Size: 1518 704 2037 2982
Fragments arranged by size:
2982 2037 1518 704
BlpI GC'TnA_GC
Cuts at: 238 955 3936 4353 238
Size: 717 2981 417 3126
Fragments arranged by size:
3126 2981 717 417
Bme1580I G_kGCm'C
Cuts at: 1024 1198 1314 1503 1532 1640 1684 2406 2628
Size: 174 116 189 29 108 44 722 222
Cuts at: 2628 4001 4045 4866 4959 6083 1024
Size: 1373 44 821 93 1124 2182
Fragments arranged by size:
2182 1373 1124 821 722 222 189 174
116 108 93 44 44 29
BmgBI CAC'GTC
Cuts at: 2039 2039
Size: 7241
BmrI ACTGGGnnnn_n'
Cuts at: 1535 4008 4453 4534 4869 7013 1535
Size: 2473 445 81 335 2144 1763
Fragments arranged by size:
2473 2144 1763 445 335 81
BmtI G_CTAG'C
Cuts at: 2479 2479
Size: 7241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3885 4085 5608 3885
Size: 200 1523 5518
Fragments arranged by size:
5518 1523 200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 868 992 2676 3483 3791 3927 4212 308
Size: 560 124 1684 807 308 136 285 3337
Fragments arranged by size:
3337 1684 807 560 308 285 136 124
Bpu10I CC'TnA_GC
Cuts at: 2173 3733 2173
Size: 1560 5681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1671 2122 3235 4417 5296 6010 6308 1671
Size: 451 1113 1182 879 714 298 2604
Fragments arranged by size:
2604 1182 1113 879 714 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 284 6945 284
Size: 6661 580
BsaAI yAC'GTr
Cuts at: 1122 1731 4116 4594 1122
Size: 609 2385 478 3769
Fragments arranged by size:
3769 2385 609 478
BsaBI GATnn'nnATC
Cuts at: 6 4755 6792 6
Size: 4749 2037 455
Fragments arranged by size:
4749 2037 455
BsaHI Gr'CG_yC
Cuts at: 401 888 1100 1237 1334 2138 2214 2486 2870
Size: 487 212 137 97 804 76 272 384
Cuts at: 2870 3371 4672 4920 5622 7118 401
Size: 501 1301 248 702 1496 524
Fragments arranged by size:
1496 1301 804 702 524 501 487 384
272 248 212 137 97 76
BsaWI w'CCGG_w
Cuts at: 1539 3073 3236 4430 4951 5629 6040 6187 6461
Size: 1534 163 1194 521 678 411 147 274
Cuts at: 6461 1539
Size: 2319
Fragments arranged by size:
2319 1534 1194 678 521 411 274 163
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 799 829 1732 1762 3782 3812 799
Size: 30 903 30 2020 30 4228
Fragments arranged by size:
4228 2020 903 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1105 1108 1417 1668 2241 3427 3685 1105
Size: 3 309 251 573 1186 258 4661
Fragments arranged by size:
4661 1186 573 309 258 251 3
BseYI C'CCAG_C
Cuts at: 118 1502 1641 2194 2240 2471 2650 2757 3872
Size: 1384 139 553 46 231 179 107 1115
Cuts at: 3872 3991 4666 5424 6089 6497 118
Size: 119 675 758 665 408 862
Fragments arranged by size:
1384 1115 862 758 675 665 553 408
231 179 139 119 107 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 970 2221 2357 3136 3990 970
Size: 1251 136 779 854 4221
Fragments arranged by size:
4221 1251 854 779 136
BsiEI CG_ry'CG
Cuts at: 104 408 632 995 1032 1202 2739 3110 3858
Size: 304 224 363 37 170 1537 371 748
Cuts at: 3858 4783 4829 6059 104
Size: 925 46 1230 1286
Fragments arranged by size:
1537 1286 1230 925 748 371 363 304
224 170 46 37
BsiHKAI G_wGCw'C
Cuts at: 239 370 841 961 1198 1447 1518 1660 1736
Size: 131 471 120 237 249 71 142 76
Cuts at: 1736 2628 2824 2852 4115 4255 5033 5223 6083
Size: 892 196 28 1263 140 778 190 860
Cuts at: 6083 239
Size: 1397
Fragments arranged by size:
1397 1263 892 860 778 471 249 237
196 190 142 140 131 120 76 71
28
BsmI GAATG_Cn'
Cuts at: 1899 1937 2602 3002 1899
Size: 38 665 400 6138
Fragments arranged by size:
6138 665 400 38
BsmBI CGTCTCn'nnnn_
Cuts at: 4029 4029
Size: 7241
Bsp1286I G_dGCh'C
Cuts at: 239 262 370 596 841 961 1024 1198 1314
Size: 23 108 226 245 120 63 174 116
Cuts at: 1314 1447 1503 1518 1532 1558 1640 1660 1684
Size: 133 56 15 14 26 82 20 24
Cuts at: 1684 1736 1993 2406 2628 2824 2852 2917 3359
Size: 52 257 413 222 196 28 65 442
Cuts at: 3359 3937 4001 4045 4115 4255 4866 4959 5033
Size: 578 64 44 70 140 611 93 74
Cuts at: 5033 5223 5285 6083 239
Size: 190 62 798 1397
Fragments arranged by size:
1397 798 611 578 442 413 257 245
226 222 196 190 174 140 133 120
116 108 93 82 74 70 65 64
63 62 56 52 44 28 26 24
23 20 15 14
BspEI T'CCGG_A
Cuts at: 1539 3073 1539
Size: 1534 5707
BspHI T'CATG_A
Cuts at: 248 336 717 753 2546 5673 248
Size: 88 381 36 1793 3127 1816
Fragments arranged by size:
3127 1816 1793 381 88 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2634 3194 4291 4505 4807 5188 2634
Size: 560 1097 214 302 381 4687
Fragments arranged by size:
4687 1097 560 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1573 3087 5533 5587 7071 7236 50
Size: 1523 1514 2446 54 1484 165 55
Fragments arranged by size:
2446 1523 1514 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 139 1169 2187 5153 139
Size: 1030 1018 2966 2227
Fragments arranged by size:
2966 2227 1030 1018
BsrFI r'CCGG_y
Cuts at: 196 1031 2210 3236 5239 5420 196
Size: 835 1179 1026 2003 181 2017
Fragments arranged by size:
2017 2003 1179 1026 835 181
BssSI C'ACGA_G
Cuts at: 834 2802 5512 6220 834
Size: 1968 2710 708 1855
Fragments arranged by size:
2710 1968 1855 708
BstAPI GCAn_nnn'nTGC
Cuts at: 772 1811 2529 4618 772
Size: 1039 718 2089 3395
Fragments arranged by size:
3395 2089 1039 718
BstBI TT'CG_AA
Cuts at: 413 1965 5602 413
Size: 1552 3637 2052
Fragments arranged by size:
3637 2052 1552
BstEII G'GTnAC_C
Cuts at: 921 1050 921
Size: 129 7112
BstYI r'GATC_y
Cuts at: 1 613 1157 1841 2062 2534 3219 3420 3588
Size: 612 544 684 221 472 685 201 168
Cuts at: 3588 4052 4266 4733 4756 5091 5337 5643 5655
Size: 464 214 467 23 335 246 306 12
Cuts at: 5655 5741 6793 1
Size: 86 1052 449
Fragments arranged by size:
1052 685 684 612 544 472 467 464
449 335 306 246 221 214 201 168
86 23 12
BtgI C'CryG_G
Cuts at: 873 1685 3110 3293 873
Size: 812 1425 183 4821
Fragments arranged by size:
4821 1425 812 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 808 5191 5372 5535 808
Size: 4383 181 163 2514
Fragments arranged by size:
4383 2514 181 163
BtsI GCAGTG_nn'
Cuts at: 1753 1819 2994 3892 4484 1753
Size: 66 1175 898 592 4510
Fragments arranged by size:
4510 1175 898 592 66
DraI TTT'AAA
Cuts at: 785 4240 785
Size: 3455 3786
DraIII CAC_nnn'GTG
Cuts at: 232 387 232
Size: 155 7086
DrdI GACnn_nn'nnGTC
Cuts at: 749 4947 6291 749
Size: 4198 1344 1699
Fragments arranged by size:
4198 1699 1344
EaeI y'GGCC_r
Cuts at: 405 629 980 992 1199 2736 3107 3299 3818
Size: 224 351 12 207 1537 371 192 519
Cuts at: 3818 3855 4118 4826 5000 5391 5418 6763 405
Size: 37 263 708 174 391 27 1345 883
Fragments arranged by size:
1537 1345 883 708 519 391 371 351
263 224 207 192 174 37 27 12
EagI C'GGCC_G
Cuts at: 405 629 992 1199 2736 3107 3855 4826 405
Size: 224 363 207 1537 371 748 971 2820
Fragments arranged by size:
2820 1537 971 748 371 363 224 207
EarI CTCTTCn'nnn_
Cuts at: 1402 1897 2747 3124 4054 5264 5474 1402
Size: 495 850 377 930 1210 210 3169
Fragments arranged by size:
3169 1210 930 850 495 377 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2623 5449 6191 6337 7051 2623
Size: 2826 742 146 714 2813
Fragments arranged by size:
2826 2813 742 714 146
EcoICRI GAG'CTC
Cuts at: 839 1445 1658 4253 839
Size: 606 213 2595 3827
Fragments arranged by size:
3827 2595 606 213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 308 416 487 868 992 993 2167 2596 2676
Size: 108 71 381 124 1 1174 429 80
Cuts at: 2676 3483 3791 3927 4212 5066 5498 5851 6513
Size: 807 308 136 285 854 432 353 662
Cuts at: 6513 308
Size: 1036
Fragments arranged by size:
1174 1036 854 807 662 432 429 381
353 308 285 136 124 108 80 71
1
EcoNI CCTnn'n_nnAGG
Cuts at: 1084 2266 2947 3428 4033 1084
Size: 1182 681 481 605 4292
Fragments arranged by size:
4292 1182 681 605 481
EcoO109I rG'GnC_Cy
Cuts at: 1419 1529 1596 2271 2402 2646 2831 3183 3998
Size: 110 67 675 131 244 185 352 815
Cuts at: 3998 4207 4380 1419
Size: 209 173 4280
Fragments arranged by size:
4280 815 675 352 244 209 185 173
131 110 67
EcoRI G'AATT_C
Cuts at: 1228 2314 4245 1228
Size: 1086 1931 4224
Fragments arranged by size:
4224 1931 1086
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1023 3066 3222 5260 1023
Size: 2043 156 2038 3004
Fragments arranged by size:
3004 2043 2038 156
FspI TGC'GCA
Cuts at: 5022 5022
Size: 7241
HaeII r_GCGC'y
Cuts at: 891 1016 1103 1716 2141 2217 2262 3374 3845
Size: 125 87 613 425 76 45 1112 471
Cuts at: 3845 3869 4675 4923 6153 6924 891
Size: 24 806 248 1230 771 1208
Fragments arranged by size:
1230 1208 1112 806 771 613 471 425
248 125 87 76 45 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 688 720 1756 1783 1788 1815 2063 2089 2095
Size: 32 1036 27 5 27 248 26 6
Cuts at: 2095 2121 2526 2558 3007 3039 3055 3087 3904
Size: 26 405 32 449 32 16 32 817
Cuts at: 3904 3936 5083 5113 5115 5145 688
Size: 32 1147 30 2 30 2784
Fragments arranged by size:
2784 1147 1036 817 449 405 248 32
32 32 32 32 30 30 27 27
26 26 16 6 5 2
HincII GTy'rAC
Cuts at: 2132 2454 4280 2132
Size: 322 1826 5093
Fragments arranged by size:
5093 1826 322
KasI G'GCGC_C
Cuts at: 887 1099 2137 2213 3370 4671 4919 887
Size: 212 1038 76 1157 1301 248 3209
Fragments arranged by size:
3209 1301 1157 1038 248 212 76
KpnI G_GTAC'C
Cuts at: 4226 4261 4226
Size: 35 7206
MluI A'CGCG_T
Cuts at: 6674 6674
Size: 7241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 630 866 878 2927 6000 6184 630
Size: 236 12 2049 3073 184 1687
Fragments arranged by size:
3073 2049 1687 236 184 12
MscI TGG'CCA
Cuts at: 3301 3820 5002 3301
Size: 519 1182 5540
Fragments arranged by size:
5540 1182 519
MslI CAynn'nnrTG
Cuts at: 341 944 1958 2693 2882 3280 5230 5357 341
Size: 603 1014 735 189 398 1950 127 2225
Fragments arranged by size:
2225 1950 1014 735 603 398 189 127
MspA1I CmG'CkG
Cuts at: 242 875 954 1379 1890 2240 2529 2586 3112
Size: 633 79 425 511 350 289 57 526
Cuts at: 3112 3399 3484 3811 3995 4352 4666 5026 5810
Size: 287 85 327 184 357 314 360 784
Cuts at: 5810 6055 242
Size: 245 1428
Fragments arranged by size:
1428 784 633 526 511 425 360 357
350 327 314 289 287 245 184 85
79 57
NaeI GCC'GGC
Cuts at: 198 2212 5422 198
Size: 2014 3210 2017
Fragments arranged by size:
3210 2017 2014
NarI GG'CG_CC
Cuts at: 888 1100 2138 2214 3371 4672 4920 888
Size: 212 1038 76 1157 1301 248 3209
Fragments arranged by size:
3209 1301 1157 1038 248 212 76
NgoMIV G'CCGG_C
Cuts at: 196 2210 5420 196
Size: 2014 3210 2017
Fragments arranged by size:
3210 2017 2014
NheI G'CTAG_C
Cuts at: 2475 2475
Size: 7241
NotI GC'GGCC_GC
Cuts at: 3107 3107
Size: 7241
NsiI A_TGCA'T
Cuts at: 2602 2602
Size: 7241
NspI r_CATG'y
Cuts at: 687 885 1308 2514 2881 4294 4794 6397 687
Size: 198 423 1206 367 1413 500 1603 1531
Fragments arranged by size:
1603 1531 1413 1206 500 423 367 198
PasI CC'CwG_GG
Cuts at: 1508 1508
Size: 7241
PciI A'CATG_T
Cuts at: 2510 6393 2510
Size: 3883 3358
PflMI CCAn_nnn'nTGG
Cuts at: 145 918 4101 145
Size: 773 3183 3285
Fragments arranged by size:
3285 3183 773
PfoI T'CCnGG_A
Cuts at: 1736 1915 2630 3011 3899 1736
Size: 179 715 381 888 5078
Fragments arranged by size:
5078 888 715 381 179
PmeI GTTT'AAAC
Cuts at: 4240 4240
Size: 7241
PmlI CAC'GTG
Cuts at: 1122 1731 4116 1122
Size: 609 2385 4247
Fragments arranged by size:
4247 2385 609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3782 3814 5687 5719 3782
Size: 32 1873 32 5304
Fragments arranged by size:
5304 1873 32 32
PpuMI rG'GwC_Cy
Cuts at: 1419 1596 2271 2646 2831 3183 4207 1419
Size: 177 675 375 185 352 1024 4453
Fragments arranged by size:
4453 1024 675 375 352 185 177
PspOMI G'GGCC_C
Cuts at: 1310 1528 2402 3997 1310
Size: 218 874 1595 4554
Fragments arranged by size:
4554 1595 874 218
PspXI vC'TCGA_Gb
Cuts at: 1446 1446
Size: 7241
PstI C_TGCA'G
Cuts at: 1259 2270 2434 2534 2959 4288 4973 1259
Size: 1011 164 100 425 1329 685 3527
Fragments arranged by size:
3527 1329 1011 685 425 164 100
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7241
PvuII CAG'CTG
Cuts at: 242 954 2240 2529 3484 3811 4666 5026 242
Size: 712 1286 289 955 327 855 360 2457
Fragments arranged by size:
2457 1286 955 855 712 360 327 289
RsrII CG'GwC_CG
Cuts at: 5436 5436
Size: 7241
SacI G_AGCT'C
Cuts at: 841 1447 1660 4255 841
Size: 606 213 2595 3827
Fragments arranged by size:
3827 2595 606 213
SacII CC_GC'GG
Cuts at: 876 3113 876
Size: 2237 5004
SalI G'TCGA_C
Cuts at: 4278 4278
Size: 7241
SapI GCTCTTCn'nnn_
Cuts at: 1402 2747 5264 5474 1402
Size: 1345 2517 210 3169
Fragments arranged by size:
3169 2517 1345 210
SbfI CC_TGCA'GG
Cuts at: 2959 4288 2959
Size: 1329 5912
ScaI AGT'ACT
Cuts at: 6758 6758
Size: 7241
SexAI A'CCwGG_T
Cuts at: 3746 4218 3746
Size: 472 6769
SfcI C'TryA_G
Cuts at: 33 1255 1857 2266 2397 2430 2530 2955 4284
Size: 1222 602 409 131 33 100 425 1329
Cuts at: 4284 4969 5937 6128 33
Size: 685 968 191 1146
Fragments arranged by size:
1329 1222 1146 968 685 602 425 409
191 131 100 33
SfoI GGC'GCC
Cuts at: 889 1101 2139 2215 3372 4673 4921 889
Size: 212 1038 76 1157 1301 248 3209
Fragments arranged by size:
3209 1301 1157 1038 248 212 76
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7241
SmaI CCC'GGG
Cuts at: 187 2407 3932 3978 4263 187
Size: 2220 1525 46 285 3165
Fragments arranged by size:
3165 2220 1525 285 46
SmlI C'TyrA_G
Cuts at: 1446 1650 2101 3250 4396 5311 6025 6287 1446
Size: 204 451 1149 1146 915 714 262 2400
Fragments arranged by size:
2400 1149 1146 915 714 451 262 204
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7241
SphI G_CATG'C
Cuts at: 885 1308 2881 4294 4794 885
Size: 423 1573 1413 500 3332
Fragments arranged by size:
3332 1573 1413 500 423
SrfI GCCC'GGGC
Cuts at: 3932 3932
Size: 7241
SspI AAT'ATT
Cuts at: 2356 2356
Size: 7241
StuI AGG'CCT
Cuts at: 127 1924 2347 2891 2953 127
Size: 1797 423 544 62 4415
Fragments arranged by size:
4415 1797 544 423 62
StyI C'CwwG_G
Cuts at: 160 1164 1611 3366 3821 4375 4729 160
Size: 1004 447 1755 455 554 354 2672
Fragments arranged by size:
2672 1755 1004 554 455 447 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1276 1426 1477 1678 2519 4824 4957 5416 5625
Size: 150 51 201 841 2305 133 459 209
Cuts at: 5625 1276
Size: 2892
Fragments arranged by size:
2892 2305 841 459 209 201 150 133
51
TatI w'GTAC_w
Cuts at: 6756 6756
Size: 7241
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1732 1764 6758 6790 1732
Size: 32 4994 32 2183
Fragments arranged by size:
4994 2183 32 32
Tth111I GACn'n_nGTC
Cuts at: 2155 2275 2485 5038 2155
Size: 120 210 2553 4358
Fragments arranged by size:
4358 2553 210 120
XbaI T'CTAG_A
Cuts at: 4272 4272
Size: 7241
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1221 3309 4736 1221
Size: 2088 1427 3726
Fragments arranged by size:
3726 2088 1427
XhoI C'TCGA_G
Cuts at: 1446 1446
Size: 7241
XmaI C'CCGG_G
Cuts at: 185 2405 3930 3976 4261 185
Size: 2220 1525 46 285 3165
Fragments arranged by size:
3165 2220 1525 285 46
XmnI GAAnn'nnTTC
Cuts at: 2314 2314
Size: 7241
ZraI GAC'GTC
Cuts at: 2487 2487
Size: 7241
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AscI BaeI BbvCI BcgI BsiWI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NruI PacI
PshAI PsiI PsrI SanDI SfiI SgrAI SnaBI SwaI