Restriction map for
pF1KB5631
(Circular) (Six-base) MAPSORT of: pF1KB5631.seq Check: 5211 from: 1 to: 6221
pF1KB5631 6221 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1656 1826 1656
Size: 170 6051
AccI GT'mk_AC
Cuts at: 227 941 1428 3259 227
Size: 714 487 1831 3189
Fragments arranged by size:
3189 1831 714 487
Acc65I G'GTAC_C
Cuts at: 396 3237 396
Size: 2841 3380
AclI AA'CG_TT
Cuts at: 6061 6061
Size: 6221
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1604 2792 3124 3173 4046 4478 4831 5493 1604
Size: 1188 332 49 873 432 353 662 2332
Fragments arranged by size:
2332 1188 873 662 432 353 332 49
AfeI AGC'GCT
Cuts at: 2092 2092
Size: 6221
AflII C'TTAA_G
Cuts at: 1674 1674
Size: 6221
AflIII A'CryG_T
Cuts at: 236 545 3573 5373 5654 236
Size: 309 3028 1800 281 803
Fragments arranged by size:
3028 1800 803 309 281
AleI CACnn'nnGTG
Cuts at: 1594 1594
Size: 6221
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 173 2051 173
Size: 1878 4343
AlwNI CAG_nnn'CTG
Cuts at: 1922 2800 3526 4964 1922
Size: 878 726 1438 3179
Fragments arranged by size:
3179 1438 878 726
ApaLI G'TGCA_C
Cuts at: 5059 5059
Size: 6221
ApoI r'AATT_y
Cuts at: 439 463 481 949 1081 1783 2556 2935 3225
Size: 24 18 468 132 702 773 379 290
Cuts at: 3225 5574 5830 439
Size: 2349 256 830
Fragments arranged by size:
2349 830 773 702 468 379 290 256
132 24 18
AseI AT'TA_AT
Cuts at: 20 407 20
Size: 387 5834
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
AvaI C'yCGr_G
Cuts at: 578 2849 3241 578
Size: 2271 392 3558
Fragments arranged by size:
3558 2271 392
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 313 346 380 413 313
Size: 33 34 33 6121
Fragments arranged by size:
6121 34 33 33
BamHI G'GATC_C
Cuts at: 2405 2919 3246 2405
Size: 514 327 5380
Fragments arranged by size:
5380 514 327
BanI G'GyrC_C
Cuts at: 396 916 1099 1811 3002 3237 3651 3899 3934
Size: 520 183 712 1191 235 414 248 35
Cuts at: 3934 396
Size: 2683
Fragments arranged by size:
2683 1191 712 520 414 248 235 183
35
BanII G_rGCy'C
Cuts at: 1494 2645 3235 4265 1494
Size: 1151 590 1030 3450
Fragments arranged by size:
3450 1151 1030 590
BbeI G_GCGC'C
Cuts at: 3655 3903 3655
Size: 248 5973
BbsI GAAGACnn'nnnn_
Cuts at: 546 1944 546
Size: 1398 4823
BbvCI CC'TCA_GC
Cuts at: 3092 3092
Size: 6221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2377 2411 2377
Size: 34 6187
BciVI GTATCCnnnnn_n'
Cuts at: 4119 5175 6221 4119
Size: 1056 1046 4119
Fragments arranged by size:
4119 1056 1046
BclI T'GATC_A
Cuts at: 3741 3741
Size: 6221
BglII A'GATC_T
Cuts at: 793 3736 5773 793
Size: 2943 2037 1241
Fragments arranged by size:
2943 2037 1241
BlpI GC'TnA_GC
Cuts at: 3333 3333
Size: 6221
Bme1580I G_kGCm'C
Cuts at: 3200 3846 3939 5063 3200
Size: 646 93 1124 4358
Fragments arranged by size:
4358 1124 646 93
BmgBI CAC'GTC
Cuts at: 237 1596 2077 237
Size: 1359 481 4381
Fragments arranged by size:
4381 1359 481
BmrI ACTGGGnnnn_n'
Cuts at: 584 1276 1971 3433 3514 3849 5993 584
Size: 692 695 1462 81 335 2144 812
Fragments arranged by size:
2144 1462 812 695 692 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 514 4588 514
Size: 4074 2147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 818
Size: 6221
Bpu10I CC'TnA_GC
Cuts at: 3071 3092 3071
Size: 21 6200
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 484 3397 4276 4990 5288 284
Size: 200 2913 879 714 298 1217
Fragments arranged by size:
2913 1217 879 714 298 200
BsaI GGTCTCn'nnnn_
Cuts at: 5925 5925
Size: 6221
BsaAI yAC'GTr
Cuts at: 3574 3574
Size: 6221
BsaBI GATnn'nnATC
Cuts at: 6 1237 3735 5772 6
Size: 1231 2498 2037 455
Fragments arranged by size:
2498 2037 1231 455
BsaHI Gr'CG_yC
Cuts at: 3652 3900 4602 6098 3652
Size: 248 702 1496 3775
Fragments arranged by size:
3775 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2999 3410 3931 4609 5020 5167 5441 2999
Size: 411 521 678 411 147 274 3779
Fragments arranged by size:
3779 678 521 411 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 209 239 1840 1870 1990 2020 2210 2240 209
Size: 30 1601 30 120 30 190 30 4190
Fragments arranged by size:
4190 1601 190 120 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 235 586 2564 3109 235
Size: 351 1978 545 3347
Fragments arranged by size:
3347 1978 545 351
BseYI C'CCAG_C
Cuts at: 244 2751 3646 4404 5069 5477 244
Size: 2507 895 758 665 408 988
Fragments arranged by size:
2507 988 895 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 128 1804 2565 128
Size: 1676 761 3784
Fragments arranged by size:
3784 1676 761
BsiEI CG_ry'CG
Cuts at: 104 3763 3809 5039 104
Size: 3659 46 1230 1286
Fragments arranged by size:
3659 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1583 2645 3235 4013 4203 5063 1583
Size: 1062 590 778 190 860 2741
Fragments arranged by size:
2741 1062 860 778 590 190
BsmI GAATG_Cn'
Cuts at: 1158 1316 1158
Size: 158 6063
BsmBI CGTCTCn'nnnn_
Cuts at: 1634 1634
Size: 6221
Bsp1286I G_dGCh'C
Cuts at: 1494 1583 2645 3200 3235 3846 3939 4013 4203
Size: 89 1062 555 35 611 93 74 190
Cuts at: 4203 4265 5063 1494
Size: 62 798 2652
Fragments arranged by size:
2652 1062 798 611 555 190 93 89
74 62 35
BspHI T'CATG_A
Cuts at: 4653 4653
Size: 6221
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1656 1826 3271 3485 3787 4168 1656
Size: 170 1445 214 302 381 3709
Fragments arranged by size:
3709 1445 381 302 214 170
BsrBI CCG'CTC
Cuts at: 50 2231 4513 4567 6051 6216 50
Size: 2181 2282 54 1484 165 55
Fragments arranged by size:
2282 2181 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 920 1217 1661 2033 2393 4133 920
Size: 297 444 372 360 1740 3008
Fragments arranged by size:
3008 1740 444 372 360 297
BsrFI r'CCGG_y
Cuts at: 2491 2840 4219 4400 2491
Size: 349 1379 181 4312
Fragments arranged by size:
4312 1379 349 181
BssSI C'ACGA_G
Cuts at: 2063 4492 5200 2063
Size: 2429 708 3084
Fragments arranged by size:
3084 2429 708
BstAPI GCAn_nnn'nTGC
Cuts at: 1818 3598 1818
Size: 1780 4441
BstBI TT'CG_AA
Cuts at: 2474 4582 2474
Size: 2108 4113
BstXI CCAn_nnnn'nTGG
Cuts at: 1096 1096
Size: 6221
BstYI r'GATC_y
Cuts at: 1 793 1349 2405 2919 3078 3110 3246 3713
Size: 792 556 1056 514 159 32 136 467
Cuts at: 3713 3736 4071 4317 4623 4635 4721 5773 1
Size: 23 335 246 306 12 86 1052 449
Fragments arranged by size:
1056 1052 792 556 514 467 449 335
306 246 159 136 86 32 23 12
BtgI C'CryG_G
Cuts at: 127 2049 3114 3200 127
Size: 1922 1065 86 3148
Fragments arranged by size:
3148 1922 1065 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2402 4171 4352 4515 2402
Size: 1769 181 163 4108
Fragments arranged by size:
4108 1769 181 163
BtsI GCAGTG_nn'
Cuts at: 145 1608 3464 145
Size: 1463 1856 2902
Fragments arranged by size:
2902 1856 1463
ClaI AT'CG_AT
Cuts at: 1122 1122
Size: 6221
DraI TTT'AAA
Cuts at: 444 1031 3220 444
Size: 587 2189 3445
Fragments arranged by size:
3445 2189 587
DrdI GACnn_nn'nnGTC
Cuts at: 3927 5271 3927
Size: 1344 4877
EaeI y'GGCC_r
Cuts at: 2838 3806 3980 4371 4398 5743 2838
Size: 968 174 391 27 1345 3316
Fragments arranged by size:
3316 1345 968 391 174 27
EagI C'GGCC_G
Cuts at: 3806 3806
Size: 6221
EarI CTCTTCn'nnn_
Cuts at: 834 1211 1223 1717 2264 4244 4454 834
Size: 377 12 494 547 1980 210 2601
Fragments arranged by size:
2601 1980 547 494 377 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2510 4429 5171 5317 6031 2510
Size: 1919 742 146 714 2700
Fragments arranged by size:
2700 1919 742 714 146
EcoICRI GAG'CTC
Cuts at: 2643 3233 2643
Size: 590 5631
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 818 1604 2792 3124 3173 4046 4478 4831 5493
Size: 786 1188 332 49 873 432 353 662
Cuts at: 5493 818
Size: 1546
Fragments arranged by size:
1546 1188 873 786 662 432 353 332
49
EcoNI CCTnn'n_nnAGG
Cuts at: 1560 1560
Size: 6221
EcoO109I rG'GnC_Cy
Cuts at: 3360 3360
Size: 6221
EcoRI G'AATT_C
Cuts at: 949 1081 3225 949
Size: 132 2144 3945
Fragments arranged by size:
3945 2144 132
EcoRV GAT'ATC
Cuts at: 2188 2188
Size: 6221
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2602 4240 2602
Size: 1638 4583
FspI TGC'GCA
Cuts at: 4002 4002
Size: 6221
HaeII r_GCGC'y
Cuts at: 2094 3655 3903 5133 5904 2094
Size: 1561 248 1230 771 2411
Fragments arranged by size:
2411 1561 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1334 1366 1453 1485 1840 1872 1990 2022 2023
Size: 32 87 32 355 32 118 32 1
Cuts at: 2023 2055 4063 4093 4095 4125 1334
Size: 32 2008 30 2 30 3430
Fragments arranged by size:
3430 2008 355 118 87 32 32 32
32 32 30 30 2 1
HincII GTy'rAC
Cuts at: 3260 3260
Size: 6221
HindIII A'AGCT_T
Cuts at: 1504 1504
Size: 6221
KasI G'GCGC_C
Cuts at: 3651 3899 3651
Size: 248 5973
KpnI G_GTAC'C
Cuts at: 400 3241 400
Size: 2841 3380
MluI A'CGCG_T
Cuts at: 5654 5654
Size: 6221
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1282 1289 4980 5164 1282
Size: 7 3691 184 2339
Fragments arranged by size:
3691 2339 184 7
MscI TGG'CCA
Cuts at: 3982 3982
Size: 6221
MslI CAynn'nnrTG
Cuts at: 1444 1594 4210 4337 1444
Size: 150 2616 127 3328
Fragments arranged by size:
3328 2616 150 127
MspA1I CmG'CkG
Cuts at: 738 914 3039 3332 3646 4006 4790 5035 738
Size: 176 2125 293 314 360 784 245 1924
Fragments arranged by size:
2125 1924 784 360 314 293 245 176
NaeI GCC'GGC
Cuts at: 4402 4402
Size: 6221
NarI GG'CG_CC
Cuts at: 3652 3900 3652
Size: 248 5973
NcoI C'CATG_G
Cuts at: 3200 3200
Size: 6221
NdeI CA'TA_TG
Cuts at: 1134 1134
Size: 6221
NgoMIV G'CCGG_C
Cuts at: 4400 4400
Size: 6221
NspI r_CATG'y
Cuts at: 1054 2638 2751 3274 3774 5377 1054
Size: 1584 113 523 500 1603 1898
Fragments arranged by size:
1898 1603 1584 523 500 113
PciI A'CATG_T
Cuts at: 5373 5373
Size: 6221
PflMI CCAn_nnn'nTGG
Cuts at: 756 1527 1922 756
Size: 771 395 5055
Fragments arranged by size:
5055 771 395
PmeI GTTT'AAAC
Cuts at: 3220 3220
Size: 6221
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4667 4699 4667
Size: 32 6189
PsiI TTA'TAA
Cuts at: 293 3052 293
Size: 2759 3462
PstI C_TGCA'G
Cuts at: 152 2046 3268 3953 152
Size: 1894 1222 685 2420
Fragments arranged by size:
2420 1894 1222 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6221
PvuII CAG'CTG
Cuts at: 738 3039 3646 4006 738
Size: 2301 607 360 2953
Fragments arranged by size:
2953 2301 607 360
RsrII CG'GwC_CG
Cuts at: 4416 4416
Size: 6221
SacI G_AGCT'C
Cuts at: 2645 3235 2645
Size: 590 5631
SalI G'TCGA_C
Cuts at: 3258 3258
Size: 6221
SapI GCTCTTCn'nnn_
Cuts at: 834 4244 4454 834
Size: 3410 210 2601
Fragments arranged by size:
3410 2601 210
SbfI CC_TGCA'GG
Cuts at: 3268 3268
Size: 6221
ScaI AGT'ACT
Cuts at: 5738 5738
Size: 6221
SfcI C'TryA_G
Cuts at: 33 148 849 1044 1073 2042 2509 2532 2863
Size: 115 701 195 29 969 467 23 331
Cuts at: 2863 3264 3949 4917 5108 33
Size: 401 685 968 191 1146
Fragments arranged by size:
1146 969 968 701 685 467 401 331
195 191 115 29 23
SfoI GGC'GCC
Cuts at: 3653 3901 3653
Size: 248 5973
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6221
SmaI CCC'GGG
Cuts at: 3243 3243
Size: 6221
SmlI C'TyrA_G
Cuts at: 299 499 578 1674 3376 4291 5005 5267 299
Size: 200 79 1096 1702 915 714 262 1253
Fragments arranged by size:
1702 1253 1096 915 714 262 200 79
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6221
SphI G_CATG'C
Cuts at: 2751 3274 3774 2751
Size: 523 500 5198
Fragments arranged by size:
5198 523 500
StyI C'CwwG_G
Cuts at: 636 2565 2883 3200 3355 3709 636
Size: 1929 318 317 155 354 3148
Fragments arranged by size:
3148 1929 354 318 317 155
SwaI ATTT'AAAT
Cuts at: 444 444
Size: 6221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2117 3804 3937 4396 4605 2117
Size: 1687 133 459 209 3733
Fragments arranged by size:
3733 1687 459 209 133
TatI w'GTAC_w
Cuts at: 503 3021 5736 503
Size: 2518 2715 988
Fragments arranged by size:
2715 2518 988
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3110 3142 5738 5770 3110
Size: 32 2596 32 3561
Fragments arranged by size:
3561 2596 32 32
Tth111I GACn'n_nGTC
Cuts at: 4018 4018
Size: 6221
XbaI T'CTAG_A
Cuts at: 3252 3252
Size: 6221
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3198 3716 3198
Size: 518 5703
XhoI C'TCGA_G
Cuts at: 578 578
Size: 6221
XmaI C'CCGG_G
Cuts at: 3241 3241
Size: 6221
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AgeI AhdI AloI ApaI AscI AvrII BfrBI
BglI BmtI BsiWI BspEI BsrGI BssHII BstEII BstZ17I
Bsu36I CspCI DraIII FseI FspAI HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI XmnI ZraI