Restriction map for
pF1KB5632
(Circular) (Six-base) MAPSORT of: pF1KB5632.seq Check: 4227 from: 1 to: 3920
pF1KB5632 3920 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 292 958 292
Size: 666 3254
Acc65I G'GTAC_C
Cuts at: 936 936
Size: 3920
AclI AA'CG_TT
Cuts at: 3760 3760
Size: 3920
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1745 2177 2924 3192 1745
Size: 432 747 268 2473
Fragments arranged by size:
2473 747 432 268
AflIII A'CryG_T
Cuts at: 1272 2376 3353 1272
Size: 1104 977 1839
Fragments arranged by size:
1839 1104 977
AgeI A'CCGG_T
Cuts at: 2308 2308
Size: 3920
AleI CACnn'nnGTG
Cuts at: 358 358
Size: 3920
AlwNI CAG_nnn'CTG
Cuts at: 886 1225 2792 886
Size: 339 1567 2014
Fragments arranged by size:
2014 1567 339
ApaI G_GGCC'C
Cuts at: 820 820
Size: 3920
ApaLI G'TGCA_C
Cuts at: 2690 2690
Size: 3920
ApoI r'AATT_y
Cuts at: 582 924 3273 3529 582
Size: 342 2349 256 973
Fragments arranged by size:
2349 973 342 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3920
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
AvaI C'yCGr_G
Cuts at: 940 940
Size: 3920
AvrII C'CTAG_G
Cuts at: 636 636
Size: 3920
BamHI G'GATC_C
Cuts at: 945 945
Size: 3920
BanI G'GyrC_C
Cuts at: 499 936 1350 1598 1633 499
Size: 437 414 248 35 2786
Fragments arranged by size:
2786 437 414 248 35
BanII G_rGCy'C
Cuts at: 197 820 934 1964 197
Size: 623 114 1030 2153
Fragments arranged by size:
2153 1030 623 114
BbeI G_GCGC'C
Cuts at: 1354 1602 1354
Size: 248 3672
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 736 770 736
Size: 34 3886
BciVI GTATCCnnnnn_n'
Cuts at: 1818 2579 3920 1818
Size: 761 1341 1818
Fragments arranged by size:
1818 1341 761
BclI T'GATC_A
Cuts at: 1440 1440
Size: 3920
BfrBI ATG'CAT
Cuts at: 460 460
Size: 3920
BglII A'GATC_T
Cuts at: 1435 3472 1435
Size: 2037 1883
BlpI GC'TnA_GC
Cuts at: 1032 1032
Size: 3920
Bme1580I G_kGCm'C
Cuts at: 820 1545 1638 2694 820
Size: 725 93 1056 2046
Fragments arranged by size:
2046 1056 725 93
BmrI ACTGGGnnnn_n'
Cuts at: 814 1132 1213 1548 3692 814
Size: 318 81 335 2144 1042
Fragments arranged by size:
2144 1042 335 318 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2287 2287
Size: 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 348 458 227
Size: 121 110 3689
Fragments arranged by size:
3689 121 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1975 2467 2765 1096
Size: 879 492 298 2251
Fragments arranged by size:
2251 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 434 646 3624 434
Size: 212 2978 730
Fragments arranged by size:
2978 730 212
BsaAI yAC'GTr
Cuts at: 1273 1273
Size: 3920
BsaBI GATnn'nnATC
Cuts at: 6 1434 3471 6
Size: 1428 2037 455
Fragments arranged by size:
2037 1428 455
BsaHI Gr'CG_yC
Cuts at: 1351 1599 2301 3797 1351
Size: 248 702 1496 1474
Fragments arranged by size:
1496 1474 702 248
BsaWI w'CCGG_w
Cuts at: 1109 1630 2308 2582 2729 3140 1109
Size: 521 678 274 147 411 1889
Fragments arranged by size:
1889 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 743 743
Size: 3920
BseYI C'CCAG_C
Cuts at: 431 1345 2103 2680 3176 431
Size: 914 758 577 496 1175
Fragments arranged by size:
1175 914 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1462 1508 2716 104
Size: 1358 46 1208 1308
Fragments arranged by size:
1358 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 934 1712 1902 2694 934
Size: 778 190 792 2160
Fragments arranged by size:
2160 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 197 820 934 1545 1638 1712 1902 1964 2694
Size: 623 114 611 93 74 190 62 730
Cuts at: 2694 197
Size: 1423
Fragments arranged by size:
1423 730 623 611 190 114 93 74
62
BspEI T'CCGG_A
Cuts at: 3140 3140
Size: 3920
BspHI T'CATG_A
Cuts at: 3096 3096
Size: 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 286 970 1184 1486 1867 286
Size: 684 214 302 381 2339
Fragments arranged by size:
2339 684 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2212 2266 3750 3915 50
Size: 2162 54 1484 165 55
Fragments arranged by size:
2162 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1832 1832
Size: 3920
BsrFI r'CCGG_y
Cuts at: 155 1918 2099 2308 155
Size: 1763 181 209 1767
Fragments arranged by size:
1767 1763 209 181
BssSI C'ACGA_G
Cuts at: 2191 2549 2191
Size: 358 3562
BstAPI GCAn_nnn'nTGC
Cuts at: 1297 1297
Size: 3920
BstBI TT'CG_AA
Cuts at: 2281 2281
Size: 3920
BstYI r'GATC_y
Cuts at: 1 805 945 1412 1435 1770 2016 3028 3114
Size: 804 140 467 23 335 246 1012 86
Cuts at: 3114 3126 3472 1
Size: 12 346 449
Fragments arranged by size:
1012 804 467 449 346 335 246 140
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3920
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1870 2051 2214 1870
Size: 181 163 3576
Fragments arranged by size:
3576 181 163
BtsI GCAGTG_nn'
Cuts at: 1163 1163
Size: 3920
DraI TTT'AAA
Cuts at: 417 919 417
Size: 502 3418
DrdI GACnn_nn'nnGTC
Cuts at: 538 1626 2484 538
Size: 1088 858 1974
Fragments arranged by size:
1974 1088 858
EaeI y'GGCC_r
Cuts at: 1505 1679 2070 2097 3442 1505
Size: 174 391 27 1345 1983
Fragments arranged by size:
1983 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1505 1505
Size: 3920
EarI CTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 108 866 2128 2438 2584 3730 108
Size: 758 1262 310 146 1146 298
Fragments arranged by size:
1262 1146 758 310 298 146
EcoICRI GAG'CTC
Cuts at: 932 932
Size: 3920
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 227 348 458 1745 2177 2924 3192 227
Size: 121 110 1287 432 747 268 955
Fragments arranged by size:
1287 955 747 432 268 121 110
EcoO109I rG'GnC_Cy
Cuts at: 816 1059 816
Size: 243 3677
EcoRI G'AATT_C
Cuts at: 924 924
Size: 3920
EcoRV GAT'ATC
Cuts at: 903 903
Size: 3920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 412 1939 412
Size: 1527 2393
FspI TGC'GCA
Cuts at: 1701 1701
Size: 3920
HaeII r_GCGC'y
Cuts at: 162 190 1354 1602 2624 3603 162
Size: 28 1164 248 1022 979 479
Fragments arranged by size:
1164 1022 979 479 248 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 718 750 1762 1792 1794 1824 718
Size: 32 1012 30 2 30 2814
Fragments arranged by size:
2814 1012 32 30 30 2
HincII GTy'rAC
Cuts at: 314 959 314
Size: 645 3275
KasI G'GCGC_C
Cuts at: 1350 1598 1350
Size: 248 3672
KpnI G_GTAC'C
Cuts at: 940 940
Size: 3920
MluI A'CGCG_T
Cuts at: 3353 3353
Size: 3920
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 794 2591 2775 794
Size: 1797 184 1939
Fragments arranged by size:
1939 1797 184
MscI TGG'CCA
Cuts at: 1681 1681
Size: 3920
MslI CAynn'nnrTG
Cuts at: 358 658 860 1909 2036 358
Size: 300 202 1049 127 2242
Fragments arranged by size:
2242 1049 300 202 127
MspA1I CmG'CkG
Cuts at: 205 431 1031 1345 1705 2718 2963 205
Size: 226 600 314 360 1013 245 1162
Fragments arranged by size:
1162 1013 600 360 314 245 226
NaeI GCC'GGC
Cuts at: 157 2101 157
Size: 1944 1976
NarI GG'CG_CC
Cuts at: 1351 1599 1351
Size: 248 3672
NcoI C'CATG_G
Cuts at: 107 107
Size: 3920
NgoMIV G'CCGG_C
Cuts at: 155 2099 155
Size: 1944 1976
NsiI A_TGCA'T
Cuts at: 462 462
Size: 3920
NspI r_CATG'y
Cuts at: 973 1473 2380 973
Size: 500 907 2513
Fragments arranged by size:
2513 907 500
PciI A'CATG_T
Cuts at: 2376 2376
Size: 3920
PfoI T'CCnGG_A
Cuts at: 325 325
Size: 3920
PmeI GTTT'AAAC
Cuts at: 919 919
Size: 3920
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3059
Size: 32 3888
PshAI GACnn'nnGTC
Cuts at: 319 319
Size: 3920
PspOMI G'GGCC_C
Cuts at: 816 816
Size: 3920
PstI C_TGCA'G
Cuts at: 718 967 1652 718
Size: 249 685 2986
Fragments arranged by size:
2986 685 249
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3920
PvuII CAG'CTG
Cuts at: 205 431 1345 1705 205
Size: 226 914 360 2420
Fragments arranged by size:
2420 914 360 226
RsrII CG'GwC_CG
Cuts at: 2115 2115
Size: 3920
SacI G_AGCT'C
Cuts at: 934 934
Size: 3920
SalI G'TCGA_C
Cuts at: 957 957
Size: 3920
SapI GCTCTTCn'nnn_
Cuts at: 1943 2153 1943
Size: 210 3710
SbfI CC_TGCA'GG
Cuts at: 967 967
Size: 3920
ScaI AGT'ACT
Cuts at: 3437 3437
Size: 3920
SfcI C'TryA_G
Cuts at: 33 678 714 963 1648 2641 2832 33
Size: 645 36 249 685 993 191 1121
Fragments arranged by size:
1121 993 685 645 249 191 36
SfoI GGC'GCC
Cuts at: 1352 1600 1352
Size: 248 3672
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3920
SmaI CCC'GGG
Cuts at: 942 942
Size: 3920
SmlI C'TyrA_G
Cuts at: 1075 1990 2482 2744 1075
Size: 915 492 262 2251
Fragments arranged by size:
2251 915 492 262
SpeI A'CTAG_T
Cuts at: 67 295 67
Size: 228 3692
SphI G_CATG'C
Cuts at: 973 1473 973
Size: 500 3420
StyI C'CwwG_G
Cuts at: 107 218 381 396 636 1054 1408 107
Size: 111 163 15 240 418 354 2619
Fragments arranged by size:
2619 418 354 240 163 111 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1503 1636 2095 2304 1503
Size: 133 459 209 3119
Fragments arranged by size:
3119 459 209 133
TatI w'GTAC_w
Cuts at: 718 3435 718
Size: 2717 1203
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 3888
Tth111I GACn'n_nGTC
Cuts at: 1717 1717
Size: 3920
XbaI T'CTAG_A
Cuts at: 951 951
Size: 3920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1415 1415
Size: 3920
XmaI C'CCGG_G
Cuts at: 940 940
Size: 3920
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
BaeI BbsI BbvCI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI