Restriction map for
pF1KB5634
(Circular) (Six-base) MAPSORT of: pF1KB5634.seq Check: 9550 from: 1 to: 5465
pF1KB5634 5465 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 175 2157 175
Size: 1982 3483
AccI GT'mk_AC
Cuts at: 1418 2503 1418
Size: 1085 4380
Acc65I G'GTAC_C
Cuts at: 2481 2481
Size: 5465
AclI AA'CG_TT
Cuts at: 5305 5305
Size: 5465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 709 932 1270 3290 3722 4075 4737 709
Size: 223 338 2020 432 353 662 1437
Fragments arranged by size:
2020 1437 662 432 353 338 223
AflIII A'CryG_T
Cuts at: 410 2817 4617 4898 410
Size: 2407 1800 281 977
Fragments arranged by size:
2407 1800 977 281
AleI CACnn'nnGTG
Cuts at: 922 934 1201 922
Size: 12 267 5186
Fragments arranged by size:
5186 267 12
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2026 2026
Size: 5465
AlwNI CAG_nnn'CTG
Cuts at: 2770 4208 2770
Size: 1438 4027
ApaI G_GGCC'C
Cuts at: 2213 2213
Size: 5465
ApaLI G'TGCA_C
Cuts at: 4303 4303
Size: 5465
ApoI r'AATT_y
Cuts at: 1651 2469 4818 5074 1651
Size: 818 2349 256 2042
Fragments arranged by size:
2349 2042 818 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5465
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
AvaI C'yCGr_G
Cuts at: 822 2212 2441 2485 822
Size: 1390 229 44 3802
Fragments arranged by size:
3802 1390 229 44
BamHI G'GATC_C
Cuts at: 2490 2490
Size: 5465
BanI G'GyrC_C
Cuts at: 142 236 928 1807 2347 2481 2895 3143 3178
Size: 94 692 879 540 134 414 248 35
Cuts at: 3178 142
Size: 2429
Fragments arranged by size:
2429 879 692 540 414 248 134 94
35
BanII G_rGCy'C
Cuts at: 943 971 1159 1179 1214 2213 2314 2479 3509
Size: 28 188 20 35 999 101 165 1030
Cuts at: 3509 943
Size: 2899
Fragments arranged by size:
2899 1030 999 188 165 101 35 28
20
BbeI G_GCGC'C
Cuts at: 146 240 2899 3147 146
Size: 94 2659 248 2464
Fragments arranged by size:
2659 2464 248 94
BbsI GAAGACnn'nnnn_
Cuts at: 375 920 375
Size: 545 4920
BbvCI CC'TCA_GC
Cuts at: 2335 2335
Size: 5465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5431
BciVI GTATCCnnnnn_n'
Cuts at: 1318 3363 4419 5465 1318
Size: 2045 1056 1046 1318
Fragments arranged by size:
2045 1318 1056 1046
BclI T'GATC_A
Cuts at: 1946 2985 1946
Size: 1039 4426
BglI GCCn_nnn'nGGC
Cuts at: 2130 2184 2130
Size: 54 5411
BglII A'GATC_T
Cuts at: 1604 2980 5017 1604
Size: 1376 2037 2052
Fragments arranged by size:
2052 2037 1376
BlpI GC'TnA_GC
Cuts at: 2420 2577 2420
Size: 157 5308
Bme1580I G_kGCm'C
Cuts at: 829 931 1810 2202 2213 2350 3090 3183 4307
Size: 102 879 392 11 137 740 93 1124
Cuts at: 4307 829
Size: 1987
Fragments arranged by size:
1987 1124 879 740 392 137 102 93
11
BmgBI CAC'GTC
Cuts at: 411 537 2370 411
Size: 126 1833 3506
Fragments arranged by size:
3506 1833 126
BmrI ACTGGGnnnn_n'
Cuts at: 2403 2677 2758 3093 5237 2403
Size: 274 81 335 2144 2631
Fragments arranged by size:
2631 2144 335 274 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 462 3832 462
Size: 3370 2095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 520
Size: 5465
Bpu10I CC'TnA_GC
Cuts at: 2335 2335
Size: 5465
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2641 3520 4234 4532 2641
Size: 879 714 298 3574
Fragments arranged by size:
3574 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 653 5169 653
Size: 4516 949
BsaAI yAC'GTr
Cuts at: 2818 2818
Size: 5465
BsaBI GATnn'nnATC
Cuts at: 6 2979 5016 6
Size: 2973 2037 455
Fragments arranged by size:
2973 2037 455
BsaHI Gr'CG_yC
Cuts at: 143 237 2144 2896 3144 3846 5342 143
Size: 94 1907 752 248 702 1496 266
Fragments arranged by size:
1907 1496 752 702 266 248 94
BsaWI w'CCGG_w
Cuts at: 977 1979 2654 3175 3853 4264 4411 4685 977
Size: 1002 675 521 678 411 147 274 1757
Fragments arranged by size:
1757 1002 678 675 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 356 386 2296 2326 2440 2470 356
Size: 30 1910 30 114 30 3351
Fragments arranged by size:
3351 1910 114 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 118 2062 2461 118
Size: 1944 399 3122
Fragments arranged by size:
3122 1944 399
BseYI C'CCAG_C
Cuts at: 2890 3648 4313 4721 2890
Size: 758 665 408 3634
Fragments arranged by size:
3634 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2191 2275 2191
Size: 84 5381
BsiEI CG_ry'CG
Cuts at: 104 3007 3053 4283 104
Size: 2903 46 1230 1286
Fragments arranged by size:
2903 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2096 2479 3257 3447 4307 2096
Size: 383 778 190 860 3254
Fragments arranged by size:
3254 860 778 383 190
BsmI GAATG_Cn'
Cuts at: 348 348
Size: 5465
BsmBI CGTCTCn'nnnn_
Cuts at: 401 401
Size: 5465
Bsp1286I G_dGCh'C
Cuts at: 829 931 943 971 1159 1179 1214 1810 2096
Size: 102 12 28 188 20 35 596 286
Cuts at: 2096 2202 2213 2314 2350 2479 3090 3183 3257
Size: 106 11 101 36 129 611 93 74
Cuts at: 3257 3447 3509 4307 829
Size: 190 62 798 1987
Fragments arranged by size:
1987 798 611 596 286 190 188 129
106 102 101 93 74 62 36 35
28 20 12 11
BspEI T'CCGG_A
Cuts at: 1979 1979
Size: 5465
BspHI T'CATG_A
Cuts at: 119 531 3897 119
Size: 412 3366 1687
Fragments arranged by size:
3366 1687 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 175 362 2157 2515 2729 3031 3412 175
Size: 187 1795 358 214 302 381 2228
Fragments arranged by size:
2228 1795 381 358 302 214 187
BsrBI CCG'CTC
Cuts at: 50 3757 3811 5295 5460 50
Size: 3707 54 1484 165 55
Fragments arranged by size:
3707 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3377 3377
Size: 5465
BsrFI r'CCGG_y
Cuts at: 233 3463 3644 233
Size: 3230 181 2054
Fragments arranged by size:
3230 2054 181
BssHII G'CGCG_C
Cuts at: 1994 1994
Size: 5465
BssSI C'ACGA_G
Cuts at: 2023 3736 4444 2023
Size: 1713 708 3044
Fragments arranged by size:
3044 1713 708
BstAPI GCAn_nnn'nTGC
Cuts at: 187 637 2842 187
Size: 450 2205 2810
Fragments arranged by size:
2810 2205 450
BstBI TT'CG_AA
Cuts at: 3826 3826
Size: 5465
BstEII G'GTnAC_C
Cuts at: 2034 2034
Size: 5465
BstXI CCAn_nnnn'nTGG
Cuts at: 840 1455 840
Size: 615 4850
BstYI r'GATC_y
Cuts at: 1 1604 2018 2490 2957 2980 3315 3561 3867
Size: 1603 414 472 467 23 335 246 306
Cuts at: 3867 3879 3965 5017 1
Size: 12 86 1052 449
Fragments arranged by size:
1603 1052 472 467 449 414 335 306
246 86 23 12
BtgI C'CryG_G
Cuts at: 1163 1292 1584 1769 1823 2109 2128 1163
Size: 129 292 185 54 286 19 4500
Fragments arranged by size:
4500 292 286 185 129 54 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3415 3596 3759 3415
Size: 181 163 5121
Fragments arranged by size:
5121 181 163
BtsI GCAGTG_nn'
Cuts at: 170 179 461 638 1913 2708 170
Size: 9 282 177 1275 795 2927
Fragments arranged by size:
2927 1275 795 282 177 9
ClaI AT'CG_AT
Cuts at: 713 713
Size: 5465
DraI TTT'AAA
Cuts at: 2464 2464
Size: 5465
DrdI GACnn_nn'nnGTC
Cuts at: 1414 3171 4515 1414
Size: 1757 1344 2364
Fragments arranged by size:
2364 1757 1344
EaeI y'GGCC_r
Cuts at: 830 1289 1587 1706 1826 1877 3050 3224 3615
Size: 459 298 119 120 51 1173 174 391
Cuts at: 3615 3642 4987 830
Size: 27 1345 1308
Fragments arranged by size:
1345 1308 1173 459 391 298 174 120
119 51 27
EagI C'GGCC_G
Cuts at: 3050 3050
Size: 5465
EarI CTCTTCn'nnn_
Cuts at: 275 3488 3698 275
Size: 3213 210 2042
Fragments arranged by size:
3213 2042 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 3673 4415 4561 5275 2269
Size: 1404 742 146 714 2459
Fragments arranged by size:
2459 1404 742 714 146
EcoICRI GAG'CTC
Cuts at: 2477 2477
Size: 5465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 520 709 932 1270 3290 3722 4075 4737 520
Size: 189 223 338 2020 432 353 662 1248
Fragments arranged by size:
2020 1248 662 432 353 338 223 189
EcoO109I rG'GnC_Cy
Cuts at: 608 807 957 2209 2398 2604 608
Size: 199 150 1252 189 206 3469
Fragments arranged by size:
3469 1252 206 199 189 150
EcoRI G'AATT_C
Cuts at: 2469 2469
Size: 5465
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1776 3484 1776
Size: 1708 3757
FspI TGC'GCA
Cuts at: 2165 3246 2165
Size: 1081 4384
HaeII r_GCGC'y
Cuts at: 146 240 2899 3147 4377 5148 146
Size: 94 2659 248 1230 771 463
Fragments arranged by size:
2659 1230 771 463 248 94
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 652 684 716 748 1166 1198 1316 1348 1782
Size: 32 32 32 418 32 118 32 434
Cuts at: 1782 1814 3307 3337 3339 3369 652
Size: 32 1493 30 2 30 2748
Fragments arranged by size:
2748 1493 434 418 118 32 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2504 2504
Size: 5465
HindIII A'AGCT_T
Cuts at: 747 747
Size: 5465
KasI G'GCGC_C
Cuts at: 142 236 2895 3143 142
Size: 94 2659 248 2464
Fragments arranged by size:
2659 2464 248 94
KpnI G_GTAC'C
Cuts at: 2485 2485
Size: 5465
MluI A'CGCG_T
Cuts at: 4898 4898
Size: 5465
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2384 4224 4408 2384
Size: 1840 184 3441
Fragments arranged by size:
3441 1840 184
MscI TGG'CCA
Cuts at: 1291 1589 3226 1291
Size: 298 1637 3530
Fragments arranged by size:
3530 1637 298
MslI CAynn'nnrTG
Cuts at: 275 622 922 934 1201 1942 3454 3581 275
Size: 347 300 12 267 741 1512 127 2159
Fragments arranged by size:
2159 1512 741 347 300 267 127 12
MspA1I CmG'CkG
Cuts at: 1165 1248 1593 2031 2111 2130 2576 2890 3250
Size: 83 345 438 80 19 446 314 360
Cuts at: 3250 4034 4279 1165
Size: 784 245 2351
Fragments arranged by size:
2351 784 446 438 360 345 314 245
83 80 19
NaeI GCC'GGC
Cuts at: 3646 3646
Size: 5465
NarI GG'CG_CC
Cuts at: 143 237 2896 3144 143
Size: 94 2659 248 2464
Fragments arranged by size:
2659 2464 248 94
NcoI C'CATG_G
Cuts at: 1292 1584 1292
Size: 292 5173
NgoMIV G'CCGG_C
Cuts at: 3644 3644
Size: 5465
NspI r_CATG'y
Cuts at: 2518 3018 4621 2518
Size: 500 1603 3362
Fragments arranged by size:
3362 1603 500
PasI CC'CwG_GG
Cuts at: 804 2343 804
Size: 1539 3926
PciI A'CATG_T
Cuts at: 4617 4617
Size: 5465
PflMI CCAn_nnn'nTGG
Cuts at: 1908 1908
Size: 5465
PmeI GTTT'AAAC
Cuts at: 2464 2464
Size: 5465
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 5433
PpuMI rG'GwC_Cy
Cuts at: 608 957 2398 608
Size: 349 1441 3675
Fragments arranged by size:
3675 1441 349
PshAI GACnn'nnGTC
Cuts at: 613 613
Size: 5465
PspOMI G'GGCC_C
Cuts at: 2209 2209
Size: 5465
PstI C_TGCA'G
Cuts at: 1604 2512 3197 1604
Size: 908 685 3872
Fragments arranged by size:
3872 908 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5465
PvuII CAG'CTG
Cuts at: 1248 1593 2031 2890 3250 1248
Size: 345 438 859 360 3463
Fragments arranged by size:
3463 859 438 360 345
RsrII CG'GwC_CG
Cuts at: 3660 3660
Size: 5465
SacI G_AGCT'C
Cuts at: 2479 2479
Size: 5465
SacII CC_GC'GG
Cuts at: 1166 2112 2131 1166
Size: 946 19 4500
Fragments arranged by size:
4500 946 19
SalI G'TCGA_C
Cuts at: 2502 2502
Size: 5465
SanDI GG'GwC_CC
Cuts at: 2398 2398
Size: 5465
SapI GCTCTTCn'nnn_
Cuts at: 3488 3698 3488
Size: 210 5255
SbfI CC_TGCA'GG
Cuts at: 2512 2512
Size: 5465
ScaI AGT'ACT
Cuts at: 4982 4982
Size: 5465
SfcI C'TryA_G
Cuts at: 33 1600 2508 3193 4161 4352 33
Size: 1567 908 685 968 191 1146
Fragments arranged by size:
1567 1146 968 908 685 191
SfoI GGC'GCC
Cuts at: 144 238 2897 3145 144
Size: 94 2659 248 2464
Fragments arranged by size:
2659 2464 248 94
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5465
SgrAI Cr'CCGG_yG
Cuts at: 233 233
Size: 5465
SmaI CCC'GGG
Cuts at: 2214 2443 2487 2214
Size: 229 44 5192
Fragments arranged by size:
5192 229 44
SmlI C'TyrA_G
Cuts at: 2620 3535 4249 4511 2620
Size: 915 714 262 3574
Fragments arranged by size:
3574 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5465
SphI G_CATG'C
Cuts at: 2518 3018 2518
Size: 500 4965
StyI C'CwwG_G
Cuts at: 1292 1584 1751 2599 2953 1292
Size: 292 167 848 354 3804
Fragments arranged by size:
3804 848 354 292 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 244 770 898 3048 3181 3640 3849 226
Size: 18 526 128 2150 133 459 209 1842
Fragments arranged by size:
2150 1842 526 459 209 133 128 18
TatI w'GTAC_w
Cuts at: 893 989 4980 893
Size: 96 3991 1378
Fragments arranged by size:
3991 1378 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 282 314 356 388 4982 5014 282
Size: 32 42 32 4594 32 733
Fragments arranged by size:
4594 733 42 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3262 3262
Size: 5465
XbaI T'CTAG_A
Cuts at: 2496 2496
Size: 5465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 426 1077 2960 426
Size: 651 1883 2931
Fragments arranged by size:
2931 1883 651
XmaI C'CCGG_G
Cuts at: 2212 2441 2485 2212
Size: 229 44 5192
Fragments arranged by size:
5192 229 44
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AhdI AloI AscI AvrII
BaeI BfrBI BmtI BsiWI BsrGI BstZ17I Bsu36I CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PsiI
PspXI PsrI SexAI SfiI SnaBI SrfI SspI StuI
SwaI XhoI XmnI ZraI