Restriction map for
pF1KB5637
(Circular) (Six-base) MAPSORT of: pF1KB5637.seq Check: 4828 from: 1 to: 6344
pF1KB5637 6344 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 918 1320 918
Size: 402 5942
AatII G_ACGT'C
Cuts at: 315 315
Size: 6344
AccI GT'mk_AC
Cuts at: 3382 3382
Size: 6344
Acc65I G'GTAC_C
Cuts at: 1194 3360 1194
Size: 2166 4178
AclI AA'CG_TT
Cuts at: 6184 6184
Size: 6344
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 1508 1601 2768 3084 4169 4601 5348 5616
Size: 546 93 1167 316 1085 432 747 268
Cuts at: 5616 962
Size: 1690
Fragments arranged by size:
1690 1167 1085 747 546 432 316 268
93
AflIII A'CryG_T
Cuts at: 568 3696 4800 5777 568
Size: 3128 1104 977 1135
Fragments arranged by size:
3128 1135 1104 977
AgeI A'CCGG_T
Cuts at: 4732 4732
Size: 6344
AhdI GACnn_n'nnGTC
Cuts at: 2475 2475
Size: 6344
AleI CACnn'nnGTG
Cuts at: 1090 1090
Size: 6344
AlwNI CAG_nnn'CTG
Cuts at: 910 1342 3174 3649 5216 910
Size: 432 1832 475 1567 2038
Fragments arranged by size:
2038 1832 1567 475 432
ApaI G_GGCC'C
Cuts at: 379 379
Size: 6344
ApaLI G'TGCA_C
Cuts at: 5114 5114
Size: 6344
ApoI r'AATT_y
Cuts at: 2263 2290 2592 2748 3052 3124 3191 3348 5697
Size: 27 302 156 304 72 67 157 2349
Cuts at: 5697 5953 2263
Size: 256 2654
Fragments arranged by size:
2654 2349 304 302 256 157 156 72
67 27
AseI AT'TA_AT
Cuts at: 20 1882 1895 3151 20
Size: 1862 13 1256 3213
Fragments arranged by size:
3213 1862 1256 13
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6344
AvaI C'yCGr_G
Cuts at: 153 1997 3364 153
Size: 1844 1367 3133
Fragments arranged by size:
3133 1844 1367
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1186 1219 1186
Size: 33 6311
BamHI G'GATC_C
Cuts at: 1521 2845 3369 1521
Size: 1324 524 4496
Fragments arranged by size:
4496 1324 524
BanI G'GyrC_C
Cuts at: 1194 1808 3360 3774 4022 4057 1194
Size: 614 1552 414 248 35 3481
Fragments arranged by size:
3481 1552 614 414 248 35
BanII G_rGCy'C
Cuts at: 164 379 1562 1769 3320 3358 4388 164
Size: 215 1183 207 1551 38 1030 2120
Fragments arranged by size:
2120 1551 1183 1030 215 207 38
BbeI G_GCGC'C
Cuts at: 3778 4026 3778
Size: 248 6096
BbsI GAAGACnn'nnnn_
Cuts at: 1381 1668 2416 2561 1381
Size: 287 748 145 5164
Fragments arranged by size:
5164 748 287 145
BbvCI CC'TCA_GC
Cuts at: 1154 1154
Size: 6344
BciVI GTATCCnnnnn_n'
Cuts at: 353 927 4242 5003 6344 353
Size: 574 3315 761 1341 353
Fragments arranged by size:
3315 1341 761 574 353
BclI T'GATC_A
Cuts at: 2163 3864 2163
Size: 1701 4643
BglI GCCn_nnn'nGGC
Cuts at: 1603 1603
Size: 6344
BglII A'GATC_T
Cuts at: 3859 5896 3859
Size: 2037 4307
BlpI GC'TnA_GC
Cuts at: 3456 3456
Size: 6344
Bme1580I G_kGCm'C
Cuts at: 379 3969 4062 5118 379
Size: 3590 93 1056 1605
Fragments arranged by size:
3590 1605 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3556 3637 3972 6116 3556
Size: 81 335 2144 3784
Fragments arranged by size:
3784 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4711 4711
Size: 6344
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 373 473 518 1346 2041 3012 335
Size: 38 100 45 828 695 971 3667
Fragments arranged by size:
3667 971 828 695 100 45 38
Bpu10I CC'TnA_GC
Cuts at: 163 662 680 698 716 728 734 794 800
Size: 499 18 18 18 12 6 60 6
Cuts at: 800 812 830 1154 1320 163
Size: 12 18 324 166 5187
Fragments arranged by size:
5187 499 324 166 60 18 18 18
18 12 12 6 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 3520 4399 4891 5189 207
Size: 3313 879 492 298 1362
Fragments arranged by size:
3313 1362 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 387 3112 6048 387
Size: 2725 2936 683
Fragments arranged by size:
2936 2725 683
BsaAI yAC'GTr
Cuts at: 571 2972 3697 571
Size: 2401 725 3218
Fragments arranged by size:
3218 2401 725
BsaBI GATnn'nnATC
Cuts at: 6 3858 5895 6
Size: 3852 2037 455
Fragments arranged by size:
3852 2037 455
BsaHI Gr'CG_yC
Cuts at: 312 3775 4023 4725 6221 312
Size: 3463 248 702 1496 435
Fragments arranged by size:
3463 1496 702 435 248
BsaWI w'CCGG_w
Cuts at: 3533 4054 4732 5006 5153 5564 3533
Size: 521 678 274 147 411 4313
Fragments arranged by size:
4313 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 590 620 1059 1089 1113 1143 2080 2110 590
Size: 30 439 30 24 30 937 30 4824
Fragments arranged by size:
4824 937 439 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 245 275 338 401 422 425 523 1528 1531
Size: 30 63 63 21 3 98 1005 3
Cuts at: 1531 1570 1585 2878 3015 3253 245
Size: 39 15 1293 137 238 3336
Fragments arranged by size:
3336 1293 1005 238 137 98 63 63
39 30 21 15 3 3
BseYI C'CCAG_C
Cuts at: 903 1106 2834 3769 4527 5104 5600 903
Size: 203 1728 935 758 577 496 1647
Fragments arranged by size:
1728 1647 935 758 577 496 203
BsiEI CG_ry'CG
Cuts at: 104 301 3886 3932 5140 104
Size: 197 3585 46 1208 1308
Fragments arranged by size:
3585 1308 1208 197 46
BsiHKAI G_wGCw'C
Cuts at: 164 2924 3358 4136 4326 5118 164
Size: 2760 434 778 190 792 1390
Fragments arranged by size:
2760 1390 792 778 434 190
BsmI GAATG_Cn'
Cuts at: 467 467
Size: 6344
BsmBI CGTCTCn'nnnn_
Cuts at: 1501 1928 3318 1501
Size: 427 1390 4527
Fragments arranged by size:
4527 1390 427
Bsp1286I G_dGCh'C
Cuts at: 164 379 1562 1769 2924 3320 3358 3969 4062
Size: 215 1183 207 1155 396 38 611 93
Cuts at: 4062 4136 4326 4388 5118 164
Size: 74 190 62 730 1390
Fragments arranged by size:
1390 1183 1155 730 611 396 215 207
190 93 74 62 38
BspEI T'CCGG_A
Cuts at: 5564 5564
Size: 6344
BspHI T'CATG_A
Cuts at: 5520 5520
Size: 6344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 918 1182 1320 3394 3608 3910 4291 918
Size: 264 138 2074 214 302 381 2971
Fragments arranged by size:
2971 2074 381 302 264 214 138
BsrBI CCG'CTC
Cuts at: 50 1671 4636 4690 6174 6339 50
Size: 1621 2965 54 1484 165 55
Fragments arranged by size:
2965 1621 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4256 4256
Size: 6344
BsrFI r'CCGG_y
Cuts at: 4342 4523 4732 4342
Size: 181 209 5954
Fragments arranged by size:
5954 209 181
BssSI C'ACGA_G
Cuts at: 3321 4615 4973 3321
Size: 1294 358 4692
Fragments arranged by size:
4692 1294 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 3721 1174
Size: 2547 3797
BstBI TT'CG_AA
Cuts at: 2407 4705 2407
Size: 2298 4046
BstYI r'GATC_y
Cuts at: 1 1521 2845 3003 3139 3369 3836 3859 4194
Size: 1520 1324 158 136 230 467 23 335
Cuts at: 4194 4440 5452 5538 5550 5896 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1520 1324 1012 467 449 346 335 246
230 158 136 86 23 12
BtgI C'CryG_G
Cuts at: 107 295 1642 1973 107
Size: 188 1347 331 4478
Fragments arranged by size:
4478 1347 331 188
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4294 4475 4638 4294
Size: 181 163 6000
Fragments arranged by size:
6000 181 163
BtsI GCAGTG_nn'
Cuts at: 227 3587 227
Size: 3360 2984
DraI TTT'AAA
Cuts at: 3343 3343
Size: 6344
DrdI GACnn_nn'nnGTC
Cuts at: 1827 4050 4908 1827
Size: 2223 858 3263
Fragments arranged by size:
3263 2223 858
EaeI y'GGCC_r
Cuts at: 298 2375 3929 4103 4494 4521 5866 298
Size: 2077 1554 174 391 27 1345 776
Fragments arranged by size:
2077 1554 1345 776 391 174 27
EagI C'GGCC_G
Cuts at: 298 3929 298
Size: 3631 2713
EarI CTCTTCn'nnn_
Cuts at: 1448 1484 1526 1562 1924 2765 4367 4577 1448
Size: 36 42 36 362 841 1602 210 3215
Fragments arranged by size:
3215 1602 841 362 210 42 36 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 4552 4862 5008 6154 127
Size: 4425 310 146 1146 317
Fragments arranged by size:
4425 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 162 3356 162
Size: 3194 3150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 373 473 518 962 1346 1508 1601 2041
Size: 38 100 45 444 384 162 93 440
Cuts at: 2041 2768 3012 3084 4169 4601 5348 5616 335
Size: 727 244 72 1085 432 747 268 1063
Fragments arranged by size:
1085 1063 747 727 444 440 432 384
268 244 162 100 93 72 45 38
EcoNI CCTnn'n_nnAGG
Cuts at: 350 353 350
Size: 3 6341
EcoO109I rG'GnC_Cy
Cuts at: 280 376 2813 3483 280
Size: 96 2437 670 3141
Fragments arranged by size:
3141 2437 670 96
EcoRI G'AATT_C
Cuts at: 3348 3348
Size: 6344
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2297 2893 4363 2297
Size: 596 1470 4278
Fragments arranged by size:
4278 1470 596
FspI TGC'GCA
Cuts at: 986 4125 986
Size: 3139 3205
HaeII r_GCGC'y
Cuts at: 3778 4026 5048 6027 3778
Size: 248 1022 979 4095
Fragments arranged by size:
4095 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 237 269 1113 1145 1939 1971 2461 2493 2527
Size: 32 844 32 794 32 490 32 34
Cuts at: 2527 2559 4186 4216 4218 4248 237
Size: 32 1627 30 2 30 2333
Fragments arranged by size:
2333 1627 844 794 490 34 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1749 3383 1749
Size: 1634 4710
HindIII A'AGCT_T
Cuts at: 2875 2875
Size: 6344
KasI G'GCGC_C
Cuts at: 3774 4022 3774
Size: 248 6096
KpnI G_GTAC'C
Cuts at: 1198 3364 1198
Size: 2166 4178
MfeI C'AATT_G
Cuts at: 2330 2330
Size: 6344
MluI A'CGCG_T
Cuts at: 5777 5777
Size: 6344
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 855 1843 5015 5199 855
Size: 988 3172 184 2000
Fragments arranged by size:
3172 2000 988 184
MscI TGG'CCA
Cuts at: 4105 4105
Size: 6344
MslI CAynn'nnrTG
Cuts at: 1090 2970 4333 4460 1090
Size: 1880 1363 127 2974
Fragments arranged by size:
2974 1880 1363 127
MspA1I CmG'CkG
Cuts at: 2380 2834 3455 3769 4129 5142 5387 2380
Size: 454 621 314 360 1013 245 3337
Fragments arranged by size:
3337 1013 621 454 360 314 245
NaeI GCC'GGC
Cuts at: 4525 4525
Size: 6344
NarI GG'CG_CC
Cuts at: 3775 4023 3775
Size: 248 6096
NcoI C'CATG_G
Cuts at: 107 1642 1973 107
Size: 1535 331 4478
Fragments arranged by size:
4478 1535 331
NdeI CA'TA_TG
Cuts at: 3264 3264
Size: 6344
NgoMIV G'CCGG_C
Cuts at: 4523 4523
Size: 6344
NspI r_CATG'y
Cuts at: 1227 2052 2308 3397 3897 4804 1227
Size: 825 256 1089 500 907 2767
Fragments arranged by size:
2767 1089 907 825 500 256
PciI A'CATG_T
Cuts at: 4800 4800
Size: 6344
PflMI CCAn_nnn'nTGG
Cuts at: 265 265
Size: 6344
PfoI T'CCnGG_A
Cuts at: 351 351
Size: 6344
PmeI GTTT'AAAC
Cuts at: 3343 3343
Size: 6344
PmlI CAC'GTG
Cuts at: 571 2972 571
Size: 2401 3943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5483 5515 5483
Size: 32 6312
PpuMI rG'GwC_Cy
Cuts at: 2813 2813
Size: 6344
PsiI TTA'TAA
Cuts at: 557 1388 1685 3130 557
Size: 831 297 1445 3771
Fragments arranged by size:
3771 1445 831 297
PspOMI G'GGCC_C
Cuts at: 375 375
Size: 6344
PstI C_TGCA'G
Cuts at: 915 3391 4076 915
Size: 2476 685 3183
Fragments arranged by size:
3183 2476 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6344
PvuII CAG'CTG
Cuts at: 3769 4129 3769
Size: 360 5984
RsrII CG'GwC_CG
Cuts at: 4539 4539
Size: 6344
SacI G_AGCT'C
Cuts at: 164 3358 164
Size: 3194 3150
SalI G'TCGA_C
Cuts at: 3381 3381
Size: 6344
SapI GCTCTTCn'nnn_
Cuts at: 1484 1526 2765 4367 4577 1484
Size: 42 1239 1602 210 3251
Fragments arranged by size:
3251 1602 1239 210 42
SbfI CC_TGCA'GG
Cuts at: 3391 3391
Size: 6344
ScaI AGT'ACT
Cuts at: 3221 5861 3221
Size: 2640 3704
SfcI C'TryA_G
Cuts at: 33 911 1052 1079 3387 4072 5065 5256 33
Size: 878 141 27 2308 685 993 191 1121
Fragments arranged by size:
2308 1121 993 878 685 191 141 27
SfoI GGC'GCC
Cuts at: 3776 4024 3776
Size: 248 6096
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6344
SmaI CCC'GGG
Cuts at: 155 3366 155
Size: 3211 3133
SmlI C'TyrA_G
Cuts at: 186 3499 4414 4906 5168 186
Size: 3313 915 492 262 1362
Fragments arranged by size:
3313 1362 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6344
SphI G_CATG'C
Cuts at: 1227 2308 3397 3897 1227
Size: 1081 1089 500 3674
Fragments arranged by size:
3674 1089 1081 500
StuI AGG'CCT
Cuts at: 184 184
Size: 6344
StyI C'CwwG_G
Cuts at: 107 1642 1973 2228 3478 3832 107
Size: 1535 331 255 1250 354 2619
Fragments arranged by size:
2619 1535 1250 354 331 255
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1017 1730 1826 2363 3927 4060 4519 4728 1017
Size: 713 96 537 1564 133 459 209 2633
Fragments arranged by size:
2633 1564 713 537 459 209 133 96
TatI w'GTAC_w
Cuts at: 2570 3219 5859 2570
Size: 649 2640 3055
Fragments arranged by size:
3055 2640 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5861 5893 5861
Size: 32 6312
Tth111I GACn'n_nGTC
Cuts at: 4141 4141
Size: 6344
XbaI T'CTAG_A
Cuts at: 3375 3375
Size: 6344
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2016 3839 2016
Size: 1823 4521
XmaI C'CCGG_G
Cuts at: 153 3364 153
Size: 3211 3133
XmnI GAAnn'nnTTC
Cuts at: 2038 2038
Size: 6344
ZraI GAC'GTC
Cuts at: 313 313
Size: 6344
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PsiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AlfI AloI AscI AvrII BcgI BfrBI
BmgBI BmtI BsgI BsiWI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI NheI NotI NruI NsiI PacI PasI PshAI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI