Restriction map for
pF1KB5639
(Circular) (Six-base) MAPSORT of: pF1KB5639.seq Check: 2156 from: 1 to: 4433
pF1KB5639 4433 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1471 1471
Size: 4433
Acc65I G'GTAC_C
Cuts at: 1449 1449
Size: 4433
AclI AA'CG_TT
Cuts at: 4273 4273
Size: 4433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2690 3043 3705 2258
Size: 432 353 662 2986
Fragments arranged by size:
2986 662 432 353
AflIII A'CryG_T
Cuts at: 1010 1785 3585 3866 1010
Size: 775 1800 281 1577
Fragments arranged by size:
1800 1577 775 281
AlwNI CAG_nnn'CTG
Cuts at: 1057 1417 1738 3176 1057
Size: 360 321 1438 2314
Fragments arranged by size:
2314 1438 360 321
ApaI G_GGCC'C
Cuts at: 150 150
Size: 4433
ApaLI G'TGCA_C
Cuts at: 3271 3271
Size: 4433
ApoI r'AATT_y
Cuts at: 1437 3786 4042 1437
Size: 2349 256 1828
Fragments arranged by size:
2349 1828 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4433
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
AvaI C'yCGr_G
Cuts at: 1021 1453 1021
Size: 432 4001
BamHI G'GATC_C
Cuts at: 1458 1458
Size: 4433
BanI G'GyrC_C
Cuts at: 532 1152 1449 1863 2111 2146 532
Size: 620 297 414 248 35 2819
Fragments arranged by size:
2819 620 414 297 248 35
BanII G_rGCy'C
Cuts at: 150 203 1381 1447 2477 150
Size: 53 1178 66 1030 2106
Fragments arranged by size:
2106 1178 1030 66 53
BbeI G_GCGC'C
Cuts at: 1867 2115 1867
Size: 248 4185
BbsI GAAGACnn'nnnn_
Cuts at: 380 380
Size: 4433
BbvCI CC'TCA_GC
Cuts at: 908 1226 908
Size: 318 4115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1123 1157 1123
Size: 34 4399
BciVI GTATCCnnnnn_n'
Cuts at: 2331 3387 4433 2331
Size: 1056 1046 2331
Fragments arranged by size:
2331 1056 1046
BclI T'GATC_A
Cuts at: 272 1953 272
Size: 1681 2752
BglII A'GATC_T
Cuts at: 1948 3985 1948
Size: 2037 2396
BlpI GC'TnA_GC
Cuts at: 1545 1545
Size: 4433
Bme1580I G_kGCm'C
Cuts at: 150 1157 2058 2151 3275 150
Size: 1007 901 93 1124 1308
Fragments arranged by size:
1308 1124 1007 901 93
BmrI ACTGGGnnnn_n'
Cuts at: 1645 1726 2061 4205 1645
Size: 81 335 2144 1873
Fragments arranged by size:
2144 1873 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2800 2800
Size: 4433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 690 645
Size: 45 4388
Bpu10I CC'TnA_GC
Cuts at: 852 872 908 962 1226 852
Size: 20 36 54 264 4059
Fragments arranged by size:
4059 264 54 36 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1609 2488 3202 3500 1609
Size: 879 714 298 2542
Fragments arranged by size:
2542 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 258 1070 4137 258
Size: 812 3067 554
Fragments arranged by size:
3067 812 554
BsaAI yAC'GTr
Cuts at: 1786 1786
Size: 4433
BsaBI GATnn'nnATC
Cuts at: 6 1947 3984 6
Size: 1941 2037 455
Fragments arranged by size:
2037 1941 455
BsaHI Gr'CG_yC
Cuts at: 1864 2112 2814 4310 1864
Size: 248 702 1496 1987
Fragments arranged by size:
1987 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1622 2143 2821 3232 3379 3653 1622
Size: 521 678 411 147 274 2402
Fragments arranged by size:
2402 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 749 779 749
Size: 30 4403
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 706 1086 1089 706
Size: 380 3 4050
Fragments arranged by size:
4050 380 3
BseYI C'CCAG_C
Cuts at: 296 681 1332 1858 2616 3281 3689 296
Size: 385 651 526 758 665 408 1040
Fragments arranged by size:
1040 758 665 651 526 408 385
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 438 477 438
Size: 39 4394
BsiEI CG_ry'CG
Cuts at: 104 137 1975 2021 3251 104
Size: 33 1838 46 1230 1286
Fragments arranged by size:
1838 1286 1230 46 33
BsiHKAI G_wGCw'C
Cuts at: 227 1381 1447 2225 2415 3275 227
Size: 1154 66 778 190 860 1385
Fragments arranged by size:
1385 1154 860 778 190 66
BsmBI CGTCTCn'nnnn_
Cuts at: 688 1321 688
Size: 633 3800
Bsp1286I G_dGCh'C
Cuts at: 150 203 227 1157 1381 1447 2058 2151 2225
Size: 53 24 930 224 66 611 93 74
Cuts at: 2225 2415 2477 3275 150
Size: 190 62 798 1308
Fragments arranged by size:
1308 930 798 611 224 190 93 74
66 62 53 24
BspHI T'CATG_A
Cuts at: 1178 2865 1178
Size: 1687 2746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1483 1697 1999 2380 1483
Size: 214 302 381 3536
Fragments arranged by size:
3536 381 302 214
BsrBI CCG'CTC
Cuts at: 50 307 1176 2725 2779 4263 4428 50
Size: 257 869 1549 54 1484 165 55
Fragments arranged by size:
1549 1484 869 257 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2345 2345
Size: 4433
BsrFI r'CCGG_y
Cuts at: 1047 2431 2612 1047
Size: 1384 181 2868
Fragments arranged by size:
2868 1384 181
BsrGI T'GTAC_A
Cuts at: 616 1253 616
Size: 637 3796
BssSI C'ACGA_G
Cuts at: 2704 3412 2704
Size: 708 3725
BstAPI GCAn_nnn'nTGC
Cuts at: 395 1417 1810 395
Size: 1022 393 3018
Fragments arranged by size:
3018 1022 393
BstBI TT'CG_AA
Cuts at: 2794 2794
Size: 4433
BstEII G'GTnAC_C
Cuts at: 699 699
Size: 4433
BstXI CCAn_nnnn'nTGG
Cuts at: 597 597
Size: 4433
BstYI r'GATC_y
Cuts at: 1 1458 1925 1948 2283 2529 2835 2847 2933
Size: 1457 467 23 335 246 306 12 86
Cuts at: 2933 3985 1
Size: 1052 449
Fragments arranged by size:
1457 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 236 107
Size: 129 4304
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2383 2564 2727 2383
Size: 181 163 4089
Fragments arranged by size:
4089 181 163
BtsI GCAGTG_nn'
Cuts at: 1676 1676
Size: 4433
DraI TTT'AAA
Cuts at: 1432 1432
Size: 4433
DraIII CAC_nnn'GTG
Cuts at: 461 515 584 461
Size: 54 69 4310
Fragments arranged by size:
4310 69 54
DrdI GACnn_nn'nnGTC
Cuts at: 2139 3483 2139
Size: 1344 3089
EaeI y'GGCC_r
Cuts at: 134 2018 2192 2583 2610 3955 134
Size: 1884 174 391 27 1345 612
Fragments arranged by size:
1884 1345 612 391 174 27
EagI C'GGCC_G
Cuts at: 134 2018 134
Size: 1884 2549
EarI CTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2641 3383 3529 4243 2641
Size: 742 146 714 2831
Fragments arranged by size:
2831 742 714 146
EcoICRI GAG'CTC
Cuts at: 1379 1445 1379
Size: 66 4367
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 645 690 2258 2690 3043 3705 645
Size: 45 1568 432 353 662 1373
Fragments arranged by size:
1568 1373 662 432 353 45
EcoO109I rG'GnC_Cy
Cuts at: 146 169 207 867 957 1572 146
Size: 23 38 660 90 615 3007
Fragments arranged by size:
3007 660 615 90 38 23
EcoRI G'AATT_C
Cuts at: 1437 1437
Size: 4433
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2452 2452
Size: 4433
FspI TGC'GCA
Cuts at: 2214 2214
Size: 4433
HaeII r_GCGC'y
Cuts at: 1867 2115 3345 4116 1867
Size: 248 1230 771 2184
Fragments arranged by size:
2184 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 350 374 376 382 406 408 584 616 874
Size: 24 2 6 24 2 176 32 258
Cuts at: 874 906 2275 2305 2307 2337 350
Size: 32 1369 30 2 30 2446
Fragments arranged by size:
2446 1369 258 176 32 32 30 30
24 24 6 2 2 2
HincII GTy'rAC
Cuts at: 1008 1278 1472 1008
Size: 270 194 3969
Fragments arranged by size:
3969 270 194
KasI G'GCGC_C
Cuts at: 1863 2111 1863
Size: 248 4185
KpnI G_GTAC'C
Cuts at: 1453 1453
Size: 4433
MluI A'CGCG_T
Cuts at: 3866 3866
Size: 4433
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3192 3376 3192
Size: 184 4249
MscI TGG'CCA
Cuts at: 2194 2194
Size: 4433
MslI CAynn'nnrTG
Cuts at: 428 2422 2549 428
Size: 1994 127 2312
Fragments arranged by size:
2312 1994 127
MspA1I CmG'CkG
Cuts at: 238 259 685 1234 1544 1858 2218 3002 3247
Size: 21 426 549 310 314 360 784 245
Cuts at: 3247 238
Size: 1424
Fragments arranged by size:
1424 784 549 426 360 314 310 245
21
NaeI GCC'GGC
Cuts at: 2614 2614
Size: 4433
NarI GG'CG_CC
Cuts at: 1864 2112 1864
Size: 248 4185
NcoI C'CATG_G
Cuts at: 107 107
Size: 4433
NgoMIV G'CCGG_C
Cuts at: 2612 2612
Size: 4433
NspI r_CATG'y
Cuts at: 1014 1486 1986 3589 1014
Size: 472 500 1603 1858
Fragments arranged by size:
1858 1603 500 472
PasI CC'CwG_GG
Cuts at: 173 712 173
Size: 539 3894
PciI A'CATG_T
Cuts at: 1010 3585 1010
Size: 2575 1858
PfoI T'CCnGG_A
Cuts at: 539 980 539
Size: 441 3992
PmeI GTTT'AAAC
Cuts at: 1432 1432
Size: 4433
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2879 2911 2879
Size: 32 4401
PpuMI rG'GwC_Cy
Cuts at: 169 207 867 957 169
Size: 38 660 90 3645
Fragments arranged by size:
3645 660 90 38
PspOMI G'GGCC_C
Cuts at: 146 146
Size: 4433
PstI C_TGCA'G
Cuts at: 1480 2165 1480
Size: 685 3748
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4433
PvuII CAG'CTG
Cuts at: 259 685 1858 2218 259
Size: 426 1173 360 2474
Fragments arranged by size:
2474 1173 426 360
RsrII CG'GwC_CG
Cuts at: 2628 2628
Size: 4433
SacI G_AGCT'C
Cuts at: 1381 1447 1381
Size: 66 4367
SacII CC_GC'GG
Cuts at: 239 239
Size: 4433
SalI G'TCGA_C
Cuts at: 1470 1470
Size: 4433
SanDI GG'GwC_CC
Cuts at: 207 867 207
Size: 660 3773
SapI GCTCTTCn'nnn_
Cuts at: 2456 2666 2456
Size: 210 4223
SbfI CC_TGCA'GG
Cuts at: 1480 1480
Size: 4433
ScaI AGT'ACT
Cuts at: 3950 3950
Size: 4433
SexAI A'CCwGG_T
Cuts at: 457 457
Size: 4433
SfcI C'TryA_G
Cuts at: 33 414 1476 2161 3129 3320 33
Size: 381 1062 685 968 191 1146
Fragments arranged by size:
1146 1062 968 685 381 191
SfoI GGC'GCC
Cuts at: 1865 2113 1865
Size: 248 4185
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4433
SmaI CCC'GGG
Cuts at: 1023 1455 1023
Size: 432 4001
SmlI C'TyrA_G
Cuts at: 1588 2503 3217 3479 1588
Size: 915 714 262 2542
Fragments arranged by size:
2542 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4433
SphI G_CATG'C
Cuts at: 1486 1986 1486
Size: 500 3933
StuI AGG'CCT
Cuts at: 195 195
Size: 4433
StyI C'CwwG_G
Cuts at: 107 1322 1567 1921 107
Size: 1215 245 354 2619
Fragments arranged by size:
2619 1215 354 245
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 292 967 2016 2149 2608 2817 292
Size: 675 1049 133 459 209 1908
Fragments arranged by size:
1908 1049 675 459 209 133
TatI w'GTAC_w
Cuts at: 616 1253 3948 616
Size: 637 2695 1101
Fragments arranged by size:
2695 1101 637
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 570 602 3950 3982 570
Size: 32 3348 32 1021
Fragments arranged by size:
3348 1021 32 32
Tth111I GACn'n_nGTC
Cuts at: 916 2230 916
Size: 1314 3119
XbaI T'CTAG_A
Cuts at: 1398 1464 1398
Size: 66 4367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1928 1928
Size: 4433
XmaI C'CCGG_G
Cuts at: 1021 1453 1021
Size: 432 4001
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BfrBI BglI BmgBI BmtI
BsiWI BsmI BspEI BssHII BstZ17I Bsu36I ClaI CspCI
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PflMI PmlI PshAI
PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI