Restriction map for
pF1KB5640
(Circular) (Six-base) MAPSORT of: pF1KB5640.seq Check: 5172 from: 1 to: 4649
pF1KB5640 4649 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1085 1687 1085
Size: 602 4047
Acc65I G'GTAC_C
Cuts at: 1665 1665
Size: 4649
AclI AA'CG_TT
Cuts at: 4489 4489
Size: 4649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 949 1387 1483 2474 2906 3259 3921 736
Size: 213 438 96 991 432 353 662 1464
Fragments arranged by size:
1464 991 662 438 432 353 213 96
AflIII A'CryG_T
Cuts at: 259 2001 3801 4082 259
Size: 1742 1800 281 826
Fragments arranged by size:
1800 1742 826 281
AleI CACnn'nnGTG
Cuts at: 1603 1603
Size: 4649
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 181
Size: 32 4617
AlwNI CAG_nnn'CTG
Cuts at: 1954 3392 1954
Size: 1438 3211
ApaI G_GGCC'C
Cuts at: 821 821
Size: 4649
ApaLI G'TGCA_C
Cuts at: 3487 3487
Size: 4649
ApoI r'AATT_y
Cuts at: 1653 4002 4258 1653
Size: 2349 256 2044
Fragments arranged by size:
2349 2044 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4649
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
AvaI C'yCGr_G
Cuts at: 275 1669 275
Size: 1394 3255
BamHI G'GATC_C
Cuts at: 1674 1674
Size: 4649
BanI G'GyrC_C
Cuts at: 371 681 1665 2079 2327 2362 371
Size: 310 984 414 248 35 2658
Fragments arranged by size:
2658 984 414 310 248 35
BanII G_rGCy'C
Cuts at: 364 617 821 1258 1280 1663 2693 364
Size: 253 204 437 22 383 1030 2320
Fragments arranged by size:
2320 1030 437 383 253 204 22
BbeI G_GCGC'C
Cuts at: 375 2083 2331 375
Size: 1708 248 2693
Fragments arranged by size:
2693 1708 248
BbsI GAAGACnn'nnnn_
Cuts at: 1274 1290 1274
Size: 16 4633
BciVI GTATCCnnnnn_n'
Cuts at: 462 2547 3603 4649 462
Size: 2085 1056 1046 462
Fragments arranged by size:
2085 1056 1046 462
BclI T'GATC_A
Cuts at: 764 1179 2169 764
Size: 415 990 3244
Fragments arranged by size:
3244 990 415
BglI GCCn_nnn'nGGC
Cuts at: 448 448
Size: 4649
BglII A'GATC_T
Cuts at: 2164 4201 2164
Size: 2037 2612
BlpI GC'TnA_GC
Cuts at: 1257 1761 1257
Size: 504 4145
Bme1580I G_kGCm'C
Cuts at: 821 1014 2274 2367 3491 821
Size: 193 1260 93 1124 1979
Fragments arranged by size:
1979 1260 1124 193 93
BmgBI CAC'GTC
Cuts at: 318 318
Size: 4649
BmrI ACTGGGnnnn_n'
Cuts at: 1861 1942 2277 4421 1861
Size: 81 335 2144 2089
Fragments arranged by size:
2144 2089 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3016 3016
Size: 4649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 478 529 770 964 1044 208
Size: 270 51 241 194 80 3813
Fragments arranged by size:
3813 270 241 194 80 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1825 2704 3418 3716 1825
Size: 879 714 298 2758
Fragments arranged by size:
2758 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 1133 4353 1133
Size: 3220 1429
BsaAI yAC'GTr
Cuts at: 2002 2002
Size: 4649
BsaBI GATnn'nnATC
Cuts at: 6 211 2163 4200 6
Size: 205 1952 2037 455
Fragments arranged by size:
2037 1952 455 205
BsaHI Gr'CG_yC
Cuts at: 372 2080 2328 3030 4526 372
Size: 1708 248 702 1496 495
Fragments arranged by size:
1708 1496 702 495 248
BsaWI w'CCGG_w
Cuts at: 1838 2359 3037 3448 3595 3869 1838
Size: 521 678 411 147 274 2618
Fragments arranged by size:
2618 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 181
Size: 30 4619
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1090 1351 1090
Size: 261 4388
BseYI C'CCAG_C
Cuts at: 948 2074 2832 3497 3905 948
Size: 1126 758 665 408 1692
Fragments arranged by size:
1692 1126 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 391 323
Size: 68 4581
BsiEI CG_ry'CG
Cuts at: 104 2191 2237 3467 104
Size: 2087 46 1230 1286
Fragments arranged by size:
2087 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1663 2441 2631 3491 1663
Size: 778 190 860 2821
Fragments arranged by size:
2821 860 778 190
BsmI GAATG_Cn'
Cuts at: 1252 1252
Size: 4649
BsmBI CGTCTCn'nnnn_
Cuts at: 454 454
Size: 4649
Bsp1286I G_dGCh'C
Cuts at: 364 617 821 1014 1258 1280 1663 2274 2367
Size: 253 204 193 244 22 383 611 93
Cuts at: 2367 2441 2631 2693 3491 364
Size: 74 190 62 798 1522
Fragments arranged by size:
1522 798 611 383 253 244 204 193
190 93 74 62 22
BspHI T'CATG_A
Cuts at: 3081 3081
Size: 4649
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1071 1699 1913 2215 2596 1071
Size: 628 214 302 381 3124
Fragments arranged by size:
3124 628 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2941 2995 4479 4644 50
Size: 2891 54 1484 165 55
Fragments arranged by size:
2891 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2561 2561
Size: 4649
BsrFI r'CCGG_y
Cuts at: 159 374 874 2647 2828 159
Size: 215 500 1773 181 1980
Fragments arranged by size:
1980 1773 500 215 181
BssSI C'ACGA_G
Cuts at: 2920 3628 2920
Size: 708 3941
BstAPI GCAn_nnn'nTGC
Cuts at: 346 2026 346
Size: 1680 2969
BstBI TT'CG_AA
Cuts at: 3010 3010
Size: 4649
BstYI r'GATC_y
Cuts at: 1 1674 2141 2164 2499 2745 3051 3063 3149
Size: 1673 467 23 335 246 306 12 86
Cuts at: 3149 4201 1
Size: 1052 449
Fragments arranged by size:
1673 1052 467 449 335 306 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 128 367 643 707 1133 1262 107
Size: 21 239 276 64 426 129 3494
Fragments arranged by size:
3494 426 276 239 129 64 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2599 2780 2943 2599
Size: 181 163 4305
Fragments arranged by size:
4305 181 163
BtsI GCAGTG_nn'
Cuts at: 1007 1892 1007
Size: 885 3764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 870 905 870
Size: 35 4614
DraI TTT'AAA
Cuts at: 1548 1648 1548
Size: 100 4549
DrdI GACnn_nn'nnGTC
Cuts at: 2355 3699 2355
Size: 1344 3305
EaeI y'GGCC_r
Cuts at: 110 449 1589 2234 2408 2799 2826 4171 110
Size: 339 1140 645 174 391 27 1345 588
Fragments arranged by size:
1345 1140 645 588 391 339 174 27
EagI C'GGCC_G
Cuts at: 2234 2234
Size: 4649
EarI CTCTTCn'nnn_
Cuts at: 1285 2672 2882 1285
Size: 1387 210 3052
Fragments arranged by size:
3052 1387 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1119 2857 3599 3745 4459 1119
Size: 1738 742 146 714 1309
Fragments arranged by size:
1738 1309 742 714 146
EcoICRI GAG'CTC
Cuts at: 1661 1661
Size: 4649
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 208 478 529 736 770 949 964 1044 1387
Size: 270 51 207 34 179 15 80 343
Cuts at: 1387 1483 2474 2906 3259 3921 208
Size: 96 991 432 353 662 936
Fragments arranged by size:
991 936 662 432 353 343 270 207
179 96 80 51 34 15
EcoNI CCTnn'n_nnAGG
Cuts at: 354 354
Size: 4649
EcoO109I rG'GnC_Cy
Cuts at: 1168 1788 1168
Size: 620 4029
EcoRI G'AATT_C
Cuts at: 1653 1653
Size: 4649
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 885 1296 2668 885
Size: 411 1372 2866
Fragments arranged by size:
2866 1372 411
FspI TGC'GCA
Cuts at: 2430 2430
Size: 4649
HaeII r_GCGC'y
Cuts at: 375 2083 2331 3561 4332 375
Size: 1708 248 1230 771 692
Fragments arranged by size:
1708 1230 771 692 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2491 2521 2523 2553 2491
Size: 30 2 30 4587
Fragments arranged by size:
4587 30 30 2
HincII GTy'rAC
Cuts at: 1688 1688
Size: 4649
KasI G'GCGC_C
Cuts at: 371 2079 2327 371
Size: 1708 248 2693
Fragments arranged by size:
2693 1708 248
KpnI G_GTAC'C
Cuts at: 1669 1669
Size: 4649
MluI A'CGCG_T
Cuts at: 4082 4082
Size: 4649
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 870 3408 3592 870
Size: 2538 184 1927
Fragments arranged by size:
2538 1927 184
MscI TGG'CCA
Cuts at: 112 2410 112
Size: 2298 2351
MslI CAynn'nnrTG
Cuts at: 1603 2638 2765 1603
Size: 1035 127 3487
Fragments arranged by size:
3487 1035 127
MspA1I CmG'CkG
Cuts at: 414 1760 2074 2434 3218 3463 414
Size: 1346 314 360 784 245 1600
Fragments arranged by size:
1600 1346 784 360 314 245
NaeI GCC'GGC
Cuts at: 161 376 2830 161
Size: 215 2454 1980
Fragments arranged by size:
2454 1980 215
NarI GG'CG_CC
Cuts at: 372 2080 2328 372
Size: 1708 248 2693
Fragments arranged by size:
2693 1708 248
NcoI C'CATG_G
Cuts at: 107 367 643 707 1262 107
Size: 260 276 64 555 3494
Fragments arranged by size:
3494 555 276 260 64
NgoMIV G'CCGG_C
Cuts at: 159 374 2828 159
Size: 215 2454 1980
Fragments arranged by size:
2454 1980 215
NspI r_CATG'y
Cuts at: 263 1323 1702 2202 3805 263
Size: 1060 379 500 1603 1107
Fragments arranged by size:
1603 1107 1060 500 379
PciI A'CATG_T
Cuts at: 259 3801 259
Size: 3542 1107
PflMI CCAn_nnn'nTGG
Cuts at: 1540 1540
Size: 4649
PfoI T'CCnGG_A
Cuts at: 786 916 786
Size: 130 4519
PmeI GTTT'AAAC
Cuts at: 1648 1648
Size: 4649
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 181 213 763 795 3095 3127 181
Size: 32 550 32 2300 32 1703
Fragments arranged by size:
2300 1703 550 32 32 32
PspOMI G'GGCC_C
Cuts at: 817 817
Size: 4649
PstI C_TGCA'G
Cuts at: 1696 2381 1696
Size: 685 3964
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4649
PvuII CAG'CTG
Cuts at: 414 2074 2434 414
Size: 1660 360 2629
Fragments arranged by size:
2629 1660 360
RsrII CG'GwC_CG
Cuts at: 2844 2844
Size: 4649
SacI G_AGCT'C
Cuts at: 1663 1663
Size: 4649
SalI G'TCGA_C
Cuts at: 1686 1686
Size: 4649
SapI GCTCTTCn'nnn_
Cuts at: 1285 2672 2882 1285
Size: 1387 210 3052
Fragments arranged by size:
3052 1387 210
SbfI CC_TGCA'GG
Cuts at: 1696 1696
Size: 4649
ScaI AGT'ACT
Cuts at: 4166 4166
Size: 4649
SexAI A'CCwGG_T
Cuts at: 422 422
Size: 4649
SfcI C'TryA_G
Cuts at: 33 1692 2377 3345 3536 33
Size: 1659 685 968 191 1146
Fragments arranged by size:
1659 1146 968 685 191
SfoI GGC'GCC
Cuts at: 373 2081 2329 373
Size: 1708 248 2693
Fragments arranged by size:
2693 1708 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4649
SmaI CCC'GGG
Cuts at: 1671 1671
Size: 4649
SmlI C'TyrA_G
Cuts at: 1804 2719 3433 3695 1804
Size: 915 714 262 2758
Fragments arranged by size:
2758 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4649
SphI G_CATG'C
Cuts at: 1702 2202 1702
Size: 500 4149
StyI C'CwwG_G
Cuts at: 107 367 446 643 707 1035 1262 1783 2137
Size: 260 79 197 64 328 227 521 354
Cuts at: 2137 107
Size: 2619
Fragments arranged by size:
2619 521 354 328 260 227 197 79
64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2232 2365 2824 3033 2232
Size: 133 459 209 3848
Fragments arranged by size:
3848 459 209 133
TatI w'GTAC_w
Cuts at: 4164 4164
Size: 4649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4166 4198 4166
Size: 32 4617
Tth111I GACn'n_nGTC
Cuts at: 589 2446 589
Size: 1857 2792
XbaI T'CTAG_A
Cuts at: 1680 1680
Size: 4649
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 183 1068 2144 183
Size: 885 1076 2688
Fragments arranged by size:
2688 1076 885
XmaI C'CCGG_G
Cuts at: 1669 1669
Size: 4649
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PflMI PfoI
PmeI PpiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI Bpu10I BsiWI
BspEI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PmlI PpuMI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI