Restriction map for
pF1KB5643
(Circular) (Six-base) MAPSORT of: pF1KB5643.seq Check: 561 from: 1 to: 3329
pF1KB5643 3329 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 367 367
Size: 3329
Acc65I G'GTAC_C
Cuts at: 345 345
Size: 3329
AclI AA'CG_TT
Cuts at: 3169 3169
Size: 3329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1154 1586 2333 2601 266
Size: 888 432 747 268 994
Fragments arranged by size:
994 888 747 432 268
AflIII A'CryG_T
Cuts at: 681 1785 2762 681
Size: 1104 977 1248
Fragments arranged by size:
1248 1104 977
AgeI A'CCGG_T
Cuts at: 1717 1717
Size: 3329
AhdI GACnn_n'nnGTC
Cuts at: 225 225
Size: 3329
AlwNI CAG_nnn'CTG
Cuts at: 634 2201 634
Size: 1567 1762
ApaLI G'TGCA_C
Cuts at: 2099 2099
Size: 3329
ApoI r'AATT_y
Cuts at: 333 2682 2938 333
Size: 2349 256 724
Fragments arranged by size:
2349 724 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3329
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3329
AvaI C'yCGr_G
Cuts at: 349 349
Size: 3329
BamHI G'GATC_C
Cuts at: 354 354
Size: 3329
BanI G'GyrC_C
Cuts at: 345 759 1007 1042 345
Size: 414 248 35 2632
Fragments arranged by size:
2632 414 248 35
BanII G_rGCy'C
Cuts at: 343 1373 343
Size: 1030 2299
BbeI G_GCGC'C
Cuts at: 763 1011 763
Size: 248 3081
BciVI GTATCCnnnnn_n'
Cuts at: 1227 1988 3329 1227
Size: 761 1341 1227
Fragments arranged by size:
1341 1227 761
BclI T'GATC_A
Cuts at: 849 849
Size: 3329
BglII A'GATC_T
Cuts at: 844 2881 844
Size: 2037 1292
BlpI GC'TnA_GC
Cuts at: 441 441
Size: 3329
Bme1580I G_kGCm'C
Cuts at: 954 1047 2103 954
Size: 93 1056 2180
Fragments arranged by size:
2180 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 541 622 957 3101 541
Size: 81 335 2144 769
Fragments arranged by size:
2144 769 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1696 1696
Size: 3329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 505 1384 1876 2174 505
Size: 879 492 298 1660
Fragments arranged by size:
1660 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3033 3033
Size: 3329
BsaAI yAC'GTr
Cuts at: 682 682
Size: 3329
BsaBI GATnn'nnATC
Cuts at: 6 843 2880 6
Size: 837 2037 455
Fragments arranged by size:
2037 837 455
BsaHI Gr'CG_yC
Cuts at: 760 1008 1710 3206 760
Size: 248 702 1496 883
Fragments arranged by size:
1496 883 702 248
BsaWI w'CCGG_w
Cuts at: 518 1039 1717 1991 2138 2549 518
Size: 521 678 274 147 411 1298
Fragments arranged by size:
1298 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 754 1512 2089 2585 754
Size: 758 577 496 1498
Fragments arranged by size:
1498 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 181
Size: 3329
BsiEI CG_ry'CG
Cuts at: 104 871 917 2125 104
Size: 767 46 1208 1308
Fragments arranged by size:
1308 1208 767 46
BsiHKAI G_wGCw'C
Cuts at: 343 1121 1311 2103 343
Size: 778 190 792 1569
Fragments arranged by size:
1569 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 343 954 1047 1121 1311 1373 2103 343
Size: 611 93 74 190 62 730 1569
Fragments arranged by size:
1569 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2549 2549
Size: 3329
BspHI T'CATG_A
Cuts at: 2505 2505
Size: 3329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 379 593 895 1276 379
Size: 214 302 381 2432
Fragments arranged by size:
2432 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1621 1675 3159 3324 50
Size: 1571 54 1484 165 55
Fragments arranged by size:
1571 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1241 1241
Size: 3329
BsrFI r'CCGG_y
Cuts at: 1327 1508 1717 1327
Size: 181 209 2939
Fragments arranged by size:
2939 209 181
BssSI C'ACGA_G
Cuts at: 1600 1958 1600
Size: 358 2971
BstAPI GCAn_nnn'nTGC
Cuts at: 706 706
Size: 3329
BstBI TT'CG_AA
Cuts at: 205 1690 205
Size: 1485 1844
BstYI r'GATC_y
Cuts at: 1 354 821 844 1179 1425 2437 2523 2535
Size: 353 467 23 335 246 1012 86 12
Cuts at: 2535 2881 1
Size: 346 449
Fragments arranged by size:
1012 467 449 353 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1279 1460 1623 1279
Size: 181 163 2985
Fragments arranged by size:
2985 181 163
BtsI GCAGTG_nn'
Cuts at: 572 572
Size: 3329
DraI TTT'AAA
Cuts at: 328 328
Size: 3329
DrdI GACnn_nn'nnGTC
Cuts at: 1035 1893 1035
Size: 858 2471
EaeI y'GGCC_r
Cuts at: 914 1088 1479 1506 2851 914
Size: 174 391 27 1345 1392
Fragments arranged by size:
1392 1345 391 174 27
EagI C'GGCC_G
Cuts at: 914 914
Size: 3329
EarI CTCTTCn'nnn_
Cuts at: 1352 1562 1352
Size: 210 3119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1537 1847 1993 3139 1537
Size: 310 146 1146 1727
Fragments arranged by size:
1727 1146 310 146
EcoICRI GAG'CTC
Cuts at: 341 341
Size: 3329
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 1154 1586 2333 2601 266
Size: 888 432 747 268 994
Fragments arranged by size:
994 888 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 468 468
Size: 3329
EcoRI G'AATT_C
Cuts at: 333 333
Size: 3329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1348 1348
Size: 3329
FspI TGC'GCA
Cuts at: 1110 1110
Size: 3329
HaeII r_GCGC'y
Cuts at: 763 1011 2033 3012 763
Size: 248 1022 979 1080
Fragments arranged by size:
1080 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 211 243 1171 1201 1203 1233 211
Size: 32 928 30 2 30 2307
Fragments arranged by size:
2307 928 32 30 30 2
HincII GTy'rAC
Cuts at: 368 368
Size: 3329
KasI G'GCGC_C
Cuts at: 759 1007 759
Size: 248 3081
KpnI G_GTAC'C
Cuts at: 349 349
Size: 3329
MluI A'CGCG_T
Cuts at: 2762 2762
Size: 3329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2000 2184 2000
Size: 184 3145
MscI TGG'CCA
Cuts at: 1090 1090
Size: 3329
MslI CAynn'nnrTG
Cuts at: 1318 1445 1318
Size: 127 3202
MspA1I CmG'CkG
Cuts at: 217 440 754 1114 2127 2372 217
Size: 223 314 360 1013 245 1174
Fragments arranged by size:
1174 1013 360 314 245 223
NaeI GCC'GGC
Cuts at: 1510 1510
Size: 3329
NarI GG'CG_CC
Cuts at: 760 1008 760
Size: 248 3081
NgoMIV G'CCGG_C
Cuts at: 1508 1508
Size: 3329
NspI r_CATG'y
Cuts at: 247 382 882 1789 247
Size: 135 500 907 1787
Fragments arranged by size:
1787 907 500 135
PciI A'CATG_T
Cuts at: 1785 1785
Size: 3329
PmeI GTTT'AAAC
Cuts at: 328 328
Size: 3329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2468 2500 2468
Size: 32 3297
PstI C_TGCA'G
Cuts at: 376 1061 376
Size: 685 2644
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3329
PvuII CAG'CTG
Cuts at: 754 1114 754
Size: 360 2969
RsrII CG'GwC_CG
Cuts at: 1524 1524
Size: 3329
SacI G_AGCT'C
Cuts at: 343 343
Size: 3329
SalI G'TCGA_C
Cuts at: 366 366
Size: 3329
SapI GCTCTTCn'nnn_
Cuts at: 1352 1562 1352
Size: 210 3119
SbfI CC_TGCA'GG
Cuts at: 376 376
Size: 3329
ScaI AGT'ACT
Cuts at: 2846 2846
Size: 3329
SfcI C'TryA_G
Cuts at: 33 372 1057 2050 2241 33
Size: 339 685 993 191 1121
Fragments arranged by size:
1121 993 685 339 191
SfoI GGC'GCC
Cuts at: 761 1009 761
Size: 248 3081
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3329
SmaI CCC'GGG
Cuts at: 351 351
Size: 3329
SmlI C'TyrA_G
Cuts at: 484 1399 1891 2153 484
Size: 915 492 262 1660
Fragments arranged by size:
1660 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3329
SphI G_CATG'C
Cuts at: 382 882 382
Size: 500 2829
StyI C'CwwG_G
Cuts at: 463 817 463
Size: 354 2975
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 912 1045 1504 1713 912
Size: 133 459 209 2528
Fragments arranged by size:
2528 459 209 133
TatI w'GTAC_w
Cuts at: 2844 2844
Size: 3329
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 2846
Size: 32 3297
Tth111I GACn'n_nGTC
Cuts at: 1126 1126
Size: 3329
XbaI T'CTAG_A
Cuts at: 360 360
Size: 3329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 824 824
Size: 3329
XmaI C'CCGG_G
Cuts at: 349 349
Size: 3329
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BglI
BmgBI BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI