Restriction map for
pF1KB5645
(Circular) (Six-base) MAPSORT of: pF1KB5645.seq Check: 660 from: 1 to: 6623
pF1KB5645 6623 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3341 3341
Size: 6623
AatII G_ACGT'C
Cuts at: 650 650
Size: 6623
AccI GT'mk_AC
Cuts at: 2555 3546 3661 2555
Size: 991 115 5517
Fragments arranged by size:
5517 991 115
Acc65I G'GTAC_C
Cuts at: 3639 3639
Size: 6623
AclI AA'CG_TT
Cuts at: 6463 6463
Size: 6623
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 1034 3114 4448 4880 5627 5895 441
Size: 593 2080 1334 432 747 268 1169
Fragments arranged by size:
2080 1334 1169 747 593 432 268
AflIII A'CryG_T
Cuts at: 1456 3975 5079 6056 1456
Size: 2519 1104 977 2023
Fragments arranged by size:
2519 2023 1104 977
AgeI A'CCGG_T
Cuts at: 1925 5011 1925
Size: 3086 3537
AhdI GACnn_n'nnGTC
Cuts at: 130 130
Size: 6623
AleI CACnn'nnGTG
Cuts at: 976 1928 976
Size: 952 5671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1906 2860 1906
Size: 954 5669
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1263 1295 1263
Size: 32 6591
AlwNI CAG_nnn'CTG
Cuts at: 178 1198 3322 3928 5495 178
Size: 1020 2124 606 1567 1306
Fragments arranged by size:
2124 1567 1306 1020 606
ApaI G_GGCC'C
Cuts at: 262 603 262
Size: 341 6282
ApaLI G'TGCA_C
Cuts at: 1863 5393 1863
Size: 3530 3093
ApoI r'AATT_y
Cuts at: 1301 2345 3627 5976 6232 1301
Size: 1044 1282 2349 256 1692
Fragments arranged by size:
2349 1692 1282 1044 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6623
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6623
AvaI C'yCGr_G
Cuts at: 407 3282 3643 407
Size: 2875 361 3387
Fragments arranged by size:
3387 2875 361
BamHI G'GATC_C
Cuts at: 1481 3648 1481
Size: 2167 4456
BanI G'GyrC_C
Cuts at: 157 242 484 3136 3639 4053 4301 4336 157
Size: 85 242 2652 503 414 248 35 2444
Fragments arranged by size:
2652 2444 503 414 248 242 85 35
BanII G_rGCy'C
Cuts at: 262 603 1354 3273 3637 4667 262
Size: 341 751 1919 364 1030 2218
Fragments arranged by size:
2218 1919 1030 751 364 341
BbeI G_GCGC'C
Cuts at: 246 4057 4305 246
Size: 3811 248 2564
Fragments arranged by size:
3811 2564 248
BbsI GAAGACnn'nnnn_
Cuts at: 409 447 599 657 785 2294 3555 409
Size: 38 152 58 128 1509 1261 3477
Fragments arranged by size:
3477 1509 1261 152 128 58 38
BbvCI CC'TCA_GC
Cuts at: 496 1042 496
Size: 546 6077
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3154 3188 3379 3413 3154
Size: 34 191 34 6364
Fragments arranged by size:
6364 191 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2533 3271 4521 5282 6623 2533
Size: 738 1250 761 1341 2533
Fragments arranged by size:
2533 1341 1250 761 738
BclI T'GATC_A
Cuts at: 1472 4143 1472
Size: 2671 3952
BfrBI ATG'CAT
Cuts at: 147 147
Size: 6623
BglI GCCn_nnn'nGGC
Cuts at: 2752 2752
Size: 6623
BglII A'GATC_T
Cuts at: 1724 4138 6175 1724
Size: 2414 2037 2172
Fragments arranged by size:
2414 2172 2037
BlpI GC'TnA_GC
Cuts at: 3735 3735
Size: 6623
Bme1580I G_kGCm'C
Cuts at: 262 415 487 603 1867 2741 2849 4248 4341
Size: 153 72 116 1264 874 108 1399 93
Cuts at: 4341 5397 262
Size: 1056 1488
Fragments arranged by size:
1488 1399 1264 1056 874 153 116 108
93 72
BmrI ACTGGGnnnn_n'
Cuts at: 1674 2198 3045 3234 3258 3835 3916 4251 6395
Size: 524 847 189 24 577 81 335 2144
Cuts at: 6395 1674
Size: 1902
Fragments arranged by size:
2144 1902 847 577 524 335 189 81
24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1067 1290 1371 4990 1067
Size: 223 81 3619 2700
Fragments arranged by size:
3619 2700 223 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 789 1440 448
Size: 341 651 5631
Fragments arranged by size:
5631 651 341
Bpu10I CC'TnA_GC
Cuts at: 496 1042 3272 496
Size: 546 2230 3847
Fragments arranged by size:
3847 2230 546
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3554 3799 4678 5170 5468 3554
Size: 245 879 492 298 4709
Fragments arranged by size:
4709 879 492 298 245
BsaI GGTCTCn'nnnn_
Cuts at: 668 1259 1540 2414 2962 6327 668
Size: 591 281 874 548 3365 964
Fragments arranged by size:
3365 964 874 591 548 281
BsaAI yAC'GTr
Cuts at: 525 3976 525
Size: 3451 3172
BsaBI GATnn'nnATC
Cuts at: 6 4137 6174 6
Size: 4131 2037 455
Fragments arranged by size:
4131 2037 455
BsaHI Gr'CG_yC
Cuts at: 131 243 647 716 1568 2492 3438 4054 4302
Size: 112 404 69 852 924 946 616 248
Cuts at: 4302 5004 6500 131
Size: 702 1496 254
Fragments arranged by size:
1496 946 924 852 702 616 404 254
248 112 69
BsaWI w'CCGG_w
Cuts at: 1925 2631 3812 4333 5011 5285 5432 5843 1925
Size: 706 1181 521 678 274 147 411 2705
Fragments arranged by size:
2705 1181 706 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1625 1655 1625
Size: 30 6593
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 814 1344 1649 814
Size: 530 305 5788
Fragments arranged by size:
5788 530 305
BseYI C'CCAG_C
Cuts at: 276 602 1228 1501 1589 2385 4048 4806 5383
Size: 326 626 273 88 796 1663 758 577
Cuts at: 5383 5879 276
Size: 496 1020
Fragments arranged by size:
1663 1020 796 758 626 577 496 326
273 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 1098 2040 607
Size: 491 942 5190
Fragments arranged by size:
5190 942 491
BsiEI CG_ry'CG
Cuts at: 104 1317 4165 4211 5419 104
Size: 1213 2848 46 1208 1308
Fragments arranged by size:
2848 1308 1213 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1867 1925 3332 3637 4415 4605 5397 1867
Size: 58 1407 305 778 190 792 3093
Fragments arranged by size:
3093 1407 792 778 305 190 58
BsmI GAATG_Cn'
Cuts at: 167 639 1213 2813 167
Size: 472 574 1600 3977
Fragments arranged by size:
3977 1600 574 472
BsmBI CGTCTCn'nnnn_
Cuts at: 1663 2483 2510 3445 1663
Size: 820 27 935 4841
Fragments arranged by size:
4841 935 820 27
Bsp1286I G_dGCh'C
Cuts at: 262 415 487 603 1354 1867 1925 2741 2849
Size: 153 72 116 751 513 58 816 108
Cuts at: 2849 3273 3332 3637 4248 4341 4415 4605 4667
Size: 424 59 305 611 93 74 190 62
Cuts at: 4667 5397 262
Size: 730 1488
Fragments arranged by size:
1488 816 751 730 611 513 424 305
190 153 116 108 93 74 72 62
59 58
BspEI T'CCGG_A
Cuts at: 2631 5843 2631
Size: 3212 3411
BspHI T'CATG_A
Cuts at: 5799 5799
Size: 6623
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1381 3341 3673 3887 4189 4570 1381
Size: 1960 332 214 302 381 3434
Fragments arranged by size:
3434 1960 381 332 302 214
BsrBI CCG'CTC
Cuts at: 50 432 1319 4915 4969 6453 6618 50
Size: 382 887 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 887 382 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1302 2166 4535 1302
Size: 864 2369 3390
Fragments arranged by size:
3390 2369 864
BsrFI r'CCGG_y
Cuts at: 556 1780 1925 1981 4621 4802 5011 556
Size: 1224 145 56 2640 181 209 2168
Fragments arranged by size:
2640 2168 1224 209 181 145 56
BsrGI T'GTAC_A
Cuts at: 608 608
Size: 6623
BssSI C'ACGA_G
Cuts at: 2842 3264 4894 5252 2842
Size: 422 1630 358 4213
Fragments arranged by size:
4213 1630 422 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1697 1862 2171 4000 1697
Size: 165 309 1829 4320
Fragments arranged by size:
4320 1829 309 165
BstBI TT'CG_AA
Cuts at: 4984 4984
Size: 6623
BstEII G'GTnAC_C
Cuts at: 1601 1601
Size: 6623
BstXI CCAn_nnnn'nTGG
Cuts at: 114 678 114
Size: 564 6059
BstYI r'GATC_y
Cuts at: 1 1481 1724 3612 3648 4115 4138 4473 4719
Size: 1480 243 1888 36 467 23 335 246
Cuts at: 4719 5731 5817 5829 6175 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1888 1480 1012 467 449 346 335 246
243 86 36 23 12
Bsu36I CC'TnA_GG
Cuts at: 388 787 388
Size: 399 6224
BtgI C'CryG_G
Cuts at: 107 1258 1435 1606 2018 107
Size: 1151 177 171 412 4712
Fragments arranged by size:
4712 1151 412 177 171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 672 687 2891 4573 4754 4917 672
Size: 15 2204 1682 181 163 2378
Fragments arranged by size:
2378 2204 1682 181 163 15
BtsI GCAGTG_nn'
Cuts at: 2241 3866 2241
Size: 1625 4998
ClaI AT'CG_AT
Cuts at: 2933 2933
Size: 6623
DraI TTT'AAA
Cuts at: 1404 3622 1404
Size: 2218 4405
DraIII CAC_nnn'GTG
Cuts at: 528 1121 3386 528
Size: 593 2265 3765
Fragments arranged by size:
3765 2265 593
DrdI GACnn_nn'nnGTC
Cuts at: 4329 5187 4329
Size: 858 5765
EaeI y'GGCC_r
Cuts at: 447 558 680 885 1031 1314 1998 2021 2225
Size: 111 122 205 146 283 684 23 204
Cuts at: 2225 2703 3369 4208 4382 4773 4800 6145 447
Size: 478 666 839 174 391 27 1345 925
Fragments arranged by size:
1345 925 839 684 666 478 391 283
205 204 174 146 122 111 27 23
EagI C'GGCC_G
Cuts at: 1314 4208 1314
Size: 2894 3729
EarI CTCTTCn'nnn_
Cuts at: 562 1010 4646 4856 562
Size: 448 3636 210 2329
Fragments arranged by size:
3636 2329 448 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 197 2968 4831 5141 5287 6433 197
Size: 2771 1863 310 146 1146 387
Fragments arranged by size:
2771 1863 1146 387 310 146
EcoICRI GAG'CTC
Cuts at: 3635 3635
Size: 6623
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 441 448 789 1034 1440 3114 4448 4880 5627
Size: 7 341 245 406 1674 1334 432 747
Cuts at: 5627 5895 441
Size: 268 1169
Fragments arranged by size:
1674 1334 1169 747 432 406 341 268
245 7
EcoO109I rG'GnC_Cy
Cuts at: 258 259 599 963 1594 2066 3319 3514 3762
Size: 1 340 364 631 472 1253 195 248
Cuts at: 3762 258
Size: 3119
Fragments arranged by size:
3119 1253 631 472 364 340 248 195
1
EcoRI G'AATT_C
Cuts at: 1301 3627 1301
Size: 2326 4297
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4642 4642
Size: 6623
FspI TGC'GCA
Cuts at: 970 3379 4404 970
Size: 2409 1025 3189
Fragments arranged by size:
3189 2409 1025
FspAI rTGC'GCAy
Cuts at: 3379 3379
Size: 6623
HaeII r_GCGC'y
Cuts at: 246 4057 4305 5327 6306 246
Size: 3811 248 1022 979 563
Fragments arranged by size:
3811 1022 979 563 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 116 148 686 718 922 954 1237 1269 2507
Size: 32 538 32 204 32 283 32 1238
Cuts at: 2507 2539 2635 2667 2982 3014 3425 3457 4465
Size: 32 96 32 315 32 411 32 1008
Cuts at: 4465 4495 4497 4527 116
Size: 30 2 30 2212
Fragments arranged by size:
2212 1238 1008 538 411 315 283 204
96 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2958 3078 3662 2958
Size: 120 584 5919
Fragments arranged by size:
5919 584 120
KasI G'GCGC_C
Cuts at: 242 4053 4301 242
Size: 3811 248 2564
Fragments arranged by size:
3811 2564 248
KpnI G_GTAC'C
Cuts at: 3643 3643
Size: 6623
MfeI C'AATT_G
Cuts at: 1748 2610 1748
Size: 862 5761
MluI A'CGCG_T
Cuts at: 6056 6056
Size: 6623
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 623 2574 5294 5478 623
Size: 1951 2720 184 1768
Fragments arranged by size:
2720 1951 1768 184
MscI TGG'CCA
Cuts at: 449 682 887 1033 2227 2705 3371 4384 449
Size: 233 205 146 1194 478 666 1013 2688
Fragments arranged by size:
2688 1194 1013 666 478 233 205 146
MslI CAynn'nnrTG
Cuts at: 152 976 1928 2581 2798 2918 3472 4612 4739
Size: 824 952 653 217 120 554 1140 127
Cuts at: 4739 152
Size: 2036
Fragments arranged by size:
2036 1140 952 824 653 554 217 127
120
MspA1I CmG'CkG
Cuts at: 606 1814 2132 2426 2640 3734 4048 4408 5421
Size: 1208 318 294 214 1094 314 360 1013
Cuts at: 5421 5666 606
Size: 245 1563
Fragments arranged by size:
1563 1208 1094 1013 360 318 314 294
245 214
NaeI GCC'GGC
Cuts at: 1782 4804 1782
Size: 3022 3601
NarI GG'CG_CC
Cuts at: 243 4054 4302 243
Size: 3811 248 2564
Fragments arranged by size:
3811 2564 248
NcoI C'CATG_G
Cuts at: 107 1258 1435 1606 107
Size: 1151 177 171 5124
Fragments arranged by size:
5124 1151 177 171
NgoMIV G'CCGG_C
Cuts at: 1780 4802 1780
Size: 3022 3601
NotI GC'GGCC_GC
Cuts at: 1314 1314
Size: 6623
NsiI A_TGCA'T
Cuts at: 149 149
Size: 6623
NspI r_CATG'y
Cuts at: 151 1460 2017 3676 4176 5083 151
Size: 1309 557 1659 500 907 1691
Fragments arranged by size:
1691 1659 1309 907 557 500
PciI A'CATG_T
Cuts at: 1456 5079 1456
Size: 3623 3000
PflMI CCAn_nnn'nTGG
Cuts at: 178 339 1753 178
Size: 161 1414 5048
Fragments arranged by size:
5048 1414 161
PfoI T'CCnGG_A
Cuts at: 950 3087 950
Size: 2137 4486
PmeI GTTT'AAAC
Cuts at: 3622 3622
Size: 6623
PmlI CAC'GTG
Cuts at: 525 525
Size: 6623
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5762 5794 5762
Size: 32 6591
PpuMI rG'GwC_Cy
Cuts at: 1594 2066 3514 1594
Size: 472 1448 4703
Fragments arranged by size:
4703 1448 472
PshAI GACnn'nnGTC
Cuts at: 2366 2435 2726 2942 3398 2366
Size: 69 291 216 456 5591
Fragments arranged by size:
5591 456 291 216 69
PspOMI G'GGCC_C
Cuts at: 258 599 258
Size: 341 6282
PstI C_TGCA'G
Cuts at: 746 992 2505 2839 3670 4355 746
Size: 246 1513 334 831 685 3014
Fragments arranged by size:
3014 1513 831 685 334 246
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6623
PvuII CAG'CTG
Cuts at: 606 1814 2640 4048 4408 606
Size: 1208 826 1408 360 2821
Fragments arranged by size:
2821 1408 1208 826 360
RsrII CG'GwC_CG
Cuts at: 4818 4818
Size: 6623
SacI G_AGCT'C
Cuts at: 3637 3637
Size: 6623
SalI G'TCGA_C
Cuts at: 3660 3660
Size: 6623
SanDI GG'GwC_CC
Cuts at: 2066 2066
Size: 6623
SapI GCTCTTCn'nnn_
Cuts at: 562 4646 4856 562
Size: 4084 210 2329
Fragments arranged by size:
4084 2329 210
SbfI CC_TGCA'GG
Cuts at: 3670 3670
Size: 6623
ScaI AGT'ACT
Cuts at: 6140 6140
Size: 6623
SexAI A'CCwGG_T
Cuts at: 338 2213 338
Size: 1875 4748
SfcI C'TryA_G
Cuts at: 33 742 988 2112 2501 2835 3098 3429 3666
Size: 709 246 1124 389 334 263 331 237
Cuts at: 3666 4351 5344 5535 33
Size: 685 993 191 1121
Fragments arranged by size:
1124 1121 993 709 685 389 334 331
263 246 237 191
SfoI GGC'GCC
Cuts at: 244 4055 4303 244
Size: 3811 248 2564
Fragments arranged by size:
3811 2564 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6623
SgrAI Cr'CCGG_yG
Cuts at: 1925 1925
Size: 6623
SmaI CCC'GGG
Cuts at: 409 3645 409
Size: 3236 3387
SmlI C'TyrA_G
Cuts at: 3569 3778 4693 5185 5447 3569
Size: 209 915 492 262 4745
Fragments arranged by size:
4745 915 492 262 209
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6623
SphI G_CATG'C
Cuts at: 3676 4176 3676
Size: 500 6123
StyI C'CwwG_G
Cuts at: 107 510 1258 1435 1597 1606 2618 3757 4111
Size: 403 748 177 162 9 1012 1139 354
Cuts at: 4111 107
Size: 2619
Fragments arranged by size:
2619 1139 1012 748 403 354 177 162
9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 2986 3253 4206 4339 4798 5007 1347
Size: 1639 267 953 133 459 209 2963
Fragments arranged by size:
2963 1639 953 459 267 209 133
TatI w'GTAC_w
Cuts at: 608 1079 1792 6138 608
Size: 471 713 4346 1093
Fragments arranged by size:
4346 1093 713 471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1512 1544 1809 1841 6140 6172 1512
Size: 32 265 32 4299 32 1963
Fragments arranged by size:
4299 1963 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4420 4420
Size: 6623
XbaI T'CTAG_A
Cuts at: 3654 3654
Size: 6623
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 948 1265 2211 2767 4118 948
Size: 317 946 556 1351 3453
Fragments arranged by size:
3453 1351 946 556 317
XmaI C'CCGG_G
Cuts at: 407 3643 407
Size: 3236 3387
XmnI GAAnn'nnTTC
Cuts at: 3418 3418
Size: 6623
ZraI GAC'GTC
Cuts at: 648 648
Size: 6623
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NsiI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI AvrII BaeI BmgBI BmtI BsiWI
BssHII BstZ17I CspCI EcoNI EcoRV FseI HindIII HpaI
NdeI NheI NruI PacI PasI PsiI PspXI PsrI
SacII SfiI SnaBI SrfI SspI StuI SwaI XhoI