Restriction map for
pF1KB5649
(Circular) (Six-base) MAPSORT of: pF1KB5649.seq Check: 2772 from: 1 to: 5330
pF1KB5649 5330 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 845 2368 845
Size: 1523 3807
Acc65I G'GTAC_C
Cuts at: 571 2346 571
Size: 1775 3555
AclI AA'CG_TT
Cuts at: 137 5170 137
Size: 5033 297
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 179 972 1807 3155 3587 3940 4602 98
Size: 81 793 835 1348 432 353 662 826
Fragments arranged by size:
1348 835 826 793 662 432 353 81
AflII C'TTAA_G
Cuts at: 1093 1093
Size: 5330
AflIII A'CryG_T
Cuts at: 981 2682 4482 4763 981
Size: 1701 1800 281 1548
Fragments arranged by size:
1800 1701 1548 281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 2279 2311 2132
Size: 32 115 32 5151
Fragments arranged by size:
5151 115 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2151 2635 4073 2151
Size: 484 1438 3408
Fragments arranged by size:
3408 1438 484
ApaLI G'TGCA_C
Cuts at: 4168 4168
Size: 5330
ApoI r'AATT_y
Cuts at: 1597 2334 4683 4939 1597
Size: 737 2349 256 1988
Fragments arranged by size:
2349 1988 737 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5330
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 2350 2350
Size: 5330
BamHI G'GATC_C
Cuts at: 185 266 2096 2355 185
Size: 81 1830 259 3160
Fragments arranged by size:
3160 1830 259 81
BanI G'GyrC_C
Cuts at: 474 571 883 1098 2346 2760 3008 3043 474
Size: 97 312 215 1248 414 248 35 2761
Fragments arranged by size:
2761 1248 414 312 248 215 97 35
BanII G_rGCy'C
Cuts at: 932 961 1590 2344 3374 932
Size: 29 629 754 1030 2888
Fragments arranged by size:
2888 1030 754 629 29
BbeI G_GCGC'C
Cuts at: 2764 3012 2764
Size: 248 5082
BbsI GAAGACnn'nnnn_
Cuts at: 104 978 104
Size: 874 4456
BciVI GTATCCnnnnn_n'
Cuts at: 971 1632 3228 4284 5330 971
Size: 661 1596 1056 1046 971
Fragments arranged by size:
1596 1056 1046 971 661
BclI T'GATC_A
Cuts at: 243 1562 2850 243
Size: 1319 1288 2723
Fragments arranged by size:
2723 1319 1288
BglII A'GATC_T
Cuts at: 2845 4882 2845
Size: 2037 3293
BlpI GC'TnA_GC
Cuts at: 2442 2442
Size: 5330
Bme1580I G_kGCm'C
Cuts at: 479 2955 3048 4172 479
Size: 2476 93 1124 1637
Fragments arranged by size:
2476 1637 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 474 1214 2542 2623 2958 5102 474
Size: 740 1328 81 335 2144 702
Fragments arranged by size:
2144 1328 740 702 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 5330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 815 815
Size: 5330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2506 3385 4099 4397 2506
Size: 879 714 298 3439
Fragments arranged by size:
3439 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 229 5034 229
Size: 4805 525
BsaAI yAC'GTr
Cuts at: 2683 2683
Size: 5330
BsaBI GATnn'nnATC
Cuts at: 6 2844 4881 6
Size: 2838 2037 455
Fragments arranged by size:
2838 2037 455
BsaHI Gr'CG_yC
Cuts at: 2761 3009 3711 5207 2761
Size: 248 702 1496 2884
Fragments arranged by size:
2884 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2519 3040 3718 4129 4276 4550 2519
Size: 521 678 411 147 274 3299
Fragments arranged by size:
3299 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1614 1644 2279 2309 1614
Size: 30 635 30 4635
Fragments arranged by size:
4635 635 30 30
BseYI C'CCAG_C
Cuts at: 2755 3513 4178 4586 2755
Size: 758 665 408 3499
Fragments arranged by size:
3499 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 366 1439 1658 366
Size: 1073 219 4038
Fragments arranged by size:
4038 1073 219
BsiEI CG_ry'CG
Cuts at: 104 2872 2918 4148 104
Size: 2768 46 1230 1286
Fragments arranged by size:
2768 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 681 712 2344 3122 3312 4172 681
Size: 31 1632 778 190 860 1839
Fragments arranged by size:
1839 1632 860 778 190 31
BsmI GAATG_Cn'
Cuts at: 1891 1891
Size: 5330
Bsp1286I G_dGCh'C
Cuts at: 479 681 712 932 961 1590 2344 2955 3048
Size: 202 31 220 29 629 754 611 93
Cuts at: 3048 3122 3312 3374 4172 479
Size: 74 190 62 798 1637
Fragments arranged by size:
1637 798 754 629 611 220 202 190
93 74 62 31 29
BspHI T'CATG_A
Cuts at: 3762 3762
Size: 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2380 2594 2896 3277 2380
Size: 214 302 381 4433
Fragments arranged by size:
4433 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3622 3676 5160 5325 50
Size: 3572 54 1484 165 55
Fragments arranged by size:
3572 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 485 3242 485
Size: 2757 2573
BsrFI r'CCGG_y
Cuts at: 3328 3509 3328
Size: 181 5149
BsrGI T'GTAC_A
Cuts at: 530 530
Size: 5330
BssSI C'ACGA_G
Cuts at: 3601 4309 3601
Size: 708 4622
BstAPI GCAn_nnn'nTGC
Cuts at: 1426 2707 1426
Size: 1281 4049
BstBI TT'CG_AA
Cuts at: 3691 3691
Size: 5330
BstYI r'GATC_y
Cuts at: 1 185 266 1605 2096 2355 2822 2845 3180
Size: 184 81 1339 491 259 467 23 335
Cuts at: 3180 3426 3732 3744 3830 4882 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1339 1052 491 467 449 335 306 259
246 184 86 81 23 12
Bsu36I CC'TnA_GG
Cuts at: 687 687
Size: 5330
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 826 2266 3280 3461 3624 826
Size: 1440 1014 181 163 2532
Fragments arranged by size:
2532 1440 1014 181 163
BtsI GCAGTG_nn'
Cuts at: 403 1675 1762 2073 2573 403
Size: 1272 87 311 500 3160
Fragments arranged by size:
3160 1272 500 311 87
DraI TTT'AAA
Cuts at: 1893 2329 1893
Size: 436 4894
DrdI GACnn_nn'nnGTC
Cuts at: 3036 4380 3036
Size: 1344 3986
EaeI y'GGCC_r
Cuts at: 507 2915 3089 3480 3507 4852 507
Size: 2408 174 391 27 1345 985
Fragments arranged by size:
2408 1345 985 391 174 27
EagI C'GGCC_G
Cuts at: 2915 2915
Size: 5330
EarI CTCTTCn'nnn_
Cuts at: 1775 2236 3353 3563 1775
Size: 461 1117 210 3542
Fragments arranged by size:
3542 1117 461 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3538 4280 4426 5140 3538
Size: 742 146 714 3728
Fragments arranged by size:
3728 742 714 146
EcoICRI GAG'CTC
Cuts at: 2342 2342
Size: 5330
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 98 179 815 972 1807 3155 3587 3940 4602
Size: 81 636 157 835 1348 432 353 662
Cuts at: 4602 98
Size: 826
Fragments arranged by size:
1348 835 826 662 636 432 353 157
81
EcoNI CCTnn'n_nnAGG
Cuts at: 686 686
Size: 5330
EcoO109I rG'GnC_Cy
Cuts at: 2469 2469
Size: 5330
EcoRI G'AATT_C
Cuts at: 2334 2334
Size: 5330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2242 3349 2242
Size: 1107 4223
FspI TGC'GCA
Cuts at: 3111 3111
Size: 5330
HaeII r_GCGC'y
Cuts at: 2764 3012 4242 5013 2764
Size: 248 1230 771 3081
Fragments arranged by size:
3081 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 228 260 430 462 1996 2028 3172 3202 3204
Size: 32 170 32 1534 32 1144 30 2
Cuts at: 3204 3234 228
Size: 30 2324
Fragments arranged by size:
2324 1534 1144 170 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 636 1920 2369 636
Size: 1284 449 3597
Fragments arranged by size:
3597 1284 449
HindIII A'AGCT_T
Cuts at: 1090 1090
Size: 5330
KasI G'GCGC_C
Cuts at: 2760 3008 2760
Size: 248 5082
KpnI G_GTAC'C
Cuts at: 575 2350 575
Size: 1775 3555
MfeI C'AATT_G
Cuts at: 1568 1568
Size: 5330
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4089 4273 4089
Size: 184 5146
MscI TGG'CCA
Cuts at: 509 3091 509
Size: 2582 2748
MslI CAynn'nnrTG
Cuts at: 589 3319 3446 589
Size: 2730 127 2473
Fragments arranged by size:
2730 2473 127
MspA1I CmG'CkG
Cuts at: 157 2441 2755 3115 3899 4144 157
Size: 2284 314 360 784 245 1343
Fragments arranged by size:
2284 1343 784 360 314 245
NaeI GCC'GGC
Cuts at: 3511 3511
Size: 5330
NarI GG'CG_CC
Cuts at: 2761 3009 2761
Size: 248 5082
NgoMIV G'CCGG_C
Cuts at: 3509 3509
Size: 5330
NspI r_CATG'y
Cuts at: 985 2383 2883 4486 985
Size: 1398 500 1603 1829
Fragments arranged by size:
1829 1603 1398 500
PciI A'CATG_T
Cuts at: 981 4482 981
Size: 3501 1829
PflMI CCAn_nnn'nTGG
Cuts at: 1619 1619
Size: 5330
PfoI T'CCnGG_A
Cuts at: 2000 2000
Size: 5330
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2279 2311 3776 3808 2279
Size: 32 1465 32 3801
Fragments arranged by size:
3801 1465 32 32
PsiI TTA'TAA
Cuts at: 1175 1175
Size: 5330
PstI C_TGCA'G
Cuts at: 332 2068 2377 3062 332
Size: 1736 309 685 2600
Fragments arranged by size:
2600 1736 685 309
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 157 2755 3115 157
Size: 2598 360 2372
Fragments arranged by size:
2598 2372 360
RsrII CG'GwC_CG
Cuts at: 3525 3525
Size: 5330
SacI G_AGCT'C
Cuts at: 2344 2344
Size: 5330
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 3353 3563 3353
Size: 210 5120
SbfI CC_TGCA'GG
Cuts at: 2377 2377
Size: 5330
ScaI AGT'ACT
Cuts at: 216 608 1686 4847 216
Size: 392 1078 3161 699
Fragments arranged by size:
3161 1078 699 392
SfcI C'TryA_G
Cuts at: 33 328 646 1256 2051 2064 2235 2373 3058
Size: 295 318 610 795 13 171 138 685
Cuts at: 3058 4026 4217 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 795 685 610 318 295 191
171 138 13
SfoI GGC'GCC
Cuts at: 2762 3010 2762
Size: 248 5082
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 2352 2352
Size: 5330
SmlI C'TyrA_G
Cuts at: 1093 2485 3400 4114 4376 1093
Size: 1392 915 714 262 2047
Fragments arranged by size:
2047 1392 915 714 262
SpeI A'CTAG_T
Cuts at: 67 1414 67
Size: 1347 3983
SphI G_CATG'C
Cuts at: 2383 2883 2383
Size: 500 4830
SspI AAT'ATT
Cuts at: 2203 2203
Size: 5330
StuI AGG'CCT
Cuts at: 1697 1697
Size: 5330
StyI C'CwwG_G
Cuts at: 1554 2036 2464 2818 1554
Size: 482 428 354 4066
Fragments arranged by size:
4066 482 428 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 159 653 1233 2913 3046 3505 3714 159
Size: 494 580 1680 133 459 209 1775
Fragments arranged by size:
1775 1680 580 494 459 209 133
TatI w'GTAC_w
Cuts at: 214 530 606 1684 4845 214
Size: 316 76 1078 3161 699
Fragments arranged by size:
3161 1078 699 316 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 936 968 1069 1101 4847 4879 936
Size: 32 101 32 3746 32 1387
Fragments arranged by size:
3746 1387 101 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 2361 2361
Size: 5330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 5330
XmaI C'CCGG_G
Cuts at: 2350 2350
Size: 5330
XmnI GAAnn'nnTTC
Cuts at: 696 2225 696
Size: 1529 3801
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI Bpu10I BseRI BsiWI BsmBI BspEI BssHII BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII EcoRV FseI
FspAI HpaI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI