Restriction map for
pF1KB5651
(Circular) (Six-base) MAPSORT of: pF1KB5651.seq Check: 6388 from: 1 to: 4313
pF1KB5651 4313 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 894 1351 894
Size: 457 3856
Acc65I G'GTAC_C
Cuts at: 1329 1329
Size: 4313
AclI AA'CG_TT
Cuts at: 1211 4153 1211
Size: 2942 1371
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1256 2138 2570 3317 3585 802
Size: 454 882 432 747 268 1530
Fragments arranged by size:
1530 882 747 454 432 268
AflIII A'CryG_T
Cuts at: 1665 2769 3746 1665
Size: 1104 977 2232
Fragments arranged by size:
2232 1104 977
AgeI A'CCGG_T
Cuts at: 2701 2701
Size: 4313
AleI CACnn'nnGTG
Cuts at: 634 634
Size: 4313
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 899 899
Size: 4313
AlwNI CAG_nnn'CTG
Cuts at: 594 810 1618 3185 594
Size: 216 808 1567 1722
Fragments arranged by size:
1722 1567 808 216
ApaLI G'TGCA_C
Cuts at: 3083 3083
Size: 4313
ApoI r'AATT_y
Cuts at: 1317 3666 3922 1317
Size: 2349 256 1708
Fragments arranged by size:
2349 1708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4313
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
AvaI C'yCGr_G
Cuts at: 327 489 1333 327
Size: 162 844 3307
Fragments arranged by size:
3307 844 162
AvrII C'CTAG_G
Cuts at: 571 571
Size: 4313
BamHI G'GATC_C
Cuts at: 1338 1338
Size: 4313
BanI G'GyrC_C
Cuts at: 123 349 397 1329 1743 1991 2026 123
Size: 226 48 932 414 248 35 2410
Fragments arranged by size:
2410 932 414 248 226 48 35
BanII G_rGCy'C
Cuts at: 286 761 869 1292 1327 2357 286
Size: 475 108 423 35 1030 2242
Fragments arranged by size:
2242 1030 475 423 108 35
BbeI G_GCGC'C
Cuts at: 127 1747 1995 127
Size: 1620 248 2445
Fragments arranged by size:
2445 1620 248
BbsI GAAGACnn'nnnn_
Cuts at: 1189 1244 1189
Size: 55 4258
BbvCI CC'TCA_GC
Cuts at: 1099 1099
Size: 4313
BciVI GTATCCnnnnn_n'
Cuts at: 669 1113 2211 2972 4313 669
Size: 444 1098 761 1341 669
Fragments arranged by size:
1341 1098 761 669 444
BclI T'GATC_A
Cuts at: 1833 1833
Size: 4313
BglI GCCn_nnn'nGGC
Cuts at: 147 147
Size: 4313
BglII A'GATC_T
Cuts at: 1828 3865 1828
Size: 2037 2276
BlpI GC'TnA_GC
Cuts at: 1425 1425
Size: 4313
Bme1580I G_kGCm'C
Cuts at: 354 400 1938 2031 3087 354
Size: 46 1538 93 1056 1580
Fragments arranged by size:
1580 1538 1056 93 46
BmgBI CAC'GTC
Cuts at: 165 465 165
Size: 300 4013
BmrI ACTGGGnnnn_n'
Cuts at: 704 922 1525 1606 1941 4085 704
Size: 218 603 81 335 2144 932
Fragments arranged by size:
2144 932 603 335 218 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2680 2680
Size: 4313
Bpu10I CC'TnA_GC
Cuts at: 718 1099 718
Size: 381 3932
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1489 2368 2860 3158 1489
Size: 879 492 298 2644
Fragments arranged by size:
2644 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4017 4017
Size: 4313
BsaAI yAC'GTr
Cuts at: 1666 1666
Size: 4313
BsaBI GATnn'nnATC
Cuts at: 6 1827 3864 6
Size: 1821 2037 455
Fragments arranged by size:
2037 1821 455
BsaHI Gr'CG_yC
Cuts at: 124 1744 1992 2694 4190 124
Size: 1620 248 702 1496 247
Fragments arranged by size:
1620 1496 702 248 247
BsaWI w'CCGG_w
Cuts at: 1086 1502 2023 2701 2975 3122 3533 1086
Size: 416 521 678 274 147 411 1866
Fragments arranged by size:
1866 678 521 416 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 877 877
Size: 4313
BseYI C'CCAG_C
Cuts at: 343 353 393 494 662 1738 2496 3073 3569
Size: 10 40 101 168 1076 758 577 496
Cuts at: 3569 343
Size: 1087
Fragments arranged by size:
1087 1076 758 577 496 168 101 40
10
BsiEI CG_ry'CG
Cuts at: 104 1855 1901 3109 104
Size: 1751 46 1208 1308
Fragments arranged by size:
1751 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 869 1042 1327 2105 2295 3087 869
Size: 173 285 778 190 792 2095
Fragments arranged by size:
2095 792 778 285 190 173
BsmI GAATG_Cn'
Cuts at: 857 857
Size: 4313
Bsp1286I G_dGCh'C
Cuts at: 286 354 400 761 869 1042 1292 1327 1938
Size: 68 46 361 108 173 250 35 611
Cuts at: 1938 2031 2105 2295 2357 3087 286
Size: 93 74 190 62 730 1512
Fragments arranged by size:
1512 730 611 361 250 190 173 108
93 74 68 62 46 35
BspEI T'CCGG_A
Cuts at: 1086 3533 1086
Size: 2447 1866
BspHI T'CATG_A
Cuts at: 212 761 869 3489 212
Size: 549 108 2620 1036
Fragments arranged by size:
2620 1036 549 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1363 1577 1879 2260 1363
Size: 214 302 381 3416
Fragments arranged by size:
3416 381 302 214
BsrBI CCG'CTC
Cuts at: 50 135 365 2605 2659 4143 4308 50
Size: 85 230 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 230 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 1110 2225 1110
Size: 1115 3198
BsrFI r'CCGG_y
Cuts at: 400 2311 2492 2701 400
Size: 1911 181 209 2012
Fragments arranged by size:
2012 1911 209 181
BssSI C'ACGA_G
Cuts at: 231 673 2584 2942 231
Size: 442 1911 358 1602
Fragments arranged by size:
1911 1602 442 358
BstAPI GCAn_nnn'nTGC
Cuts at: 810 1690 810
Size: 880 3433
BstBI TT'CG_AA
Cuts at: 849 2674 849
Size: 1825 2488
BstYI r'GATC_y
Cuts at: 1 245 973 996 1338 1805 1828 2163 2409
Size: 244 728 23 342 467 23 335 246
Cuts at: 2409 3421 3507 3519 3865 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 728 467 449 346 342 335 246
244 86 23 23 12
BtgI C'CryG_G
Cuts at: 650 905 650
Size: 255 4058
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 595 2263 2444 2607 595
Size: 1668 181 163 2301
Fragments arranged by size:
2301 1668 181 163
BtsI GCAGTG_nn'
Cuts at: 481 1556 481
Size: 1075 3238
DraI TTT'AAA
Cuts at: 1312 1312
Size: 4313
DrdI GACnn_nn'nnGTC
Cuts at: 2019 2877 2019
Size: 858 3455
EaeI y'GGCC_r
Cuts at: 321 512 519 1898 2072 2463 2490 3835 321
Size: 191 7 1379 174 391 27 1345 799
Fragments arranged by size:
1379 1345 799 391 191 174 27 7
EagI C'GGCC_G
Cuts at: 1898 1898
Size: 4313
EarI CTCTTCn'nnn_
Cuts at: 205 1258 2336 2546 205
Size: 1053 1078 210 1972
Fragments arranged by size:
1972 1078 1053 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 252 436 2521 2831 2977 4123 252
Size: 184 2085 310 146 1146 442
Fragments arranged by size:
2085 1146 442 310 184 146
EcoICRI GAG'CTC
Cuts at: 867 1325 867
Size: 458 3855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 802 1256 2138 2570 3317 3585 802
Size: 454 882 432 747 268 1530
Fragments arranged by size:
1530 882 747 454 432 268
EcoO109I rG'GnC_Cy
Cuts at: 174 331 1452 174
Size: 157 1121 3035
Fragments arranged by size:
3035 1121 157
EcoRI G'AATT_C
Cuts at: 1317 1317
Size: 4313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2332 2332
Size: 4313
FspI TGC'GCA
Cuts at: 2094 2094
Size: 4313
HaeII r_GCGC'y
Cuts at: 127 1747 1995 3017 3996 127
Size: 1620 248 1022 979 444
Fragments arranged by size:
1620 1022 979 444 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 268 300 958 990 1252 1284 2155 2185 2187
Size: 32 658 32 262 32 871 30 2
Cuts at: 2187 2217 268
Size: 30 2364
Fragments arranged by size:
2364 871 658 262 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 895 945 1215 1352 895
Size: 50 270 137 3856
Fragments arranged by size:
3856 270 137 50
KasI G'GCGC_C
Cuts at: 123 1743 1991 123
Size: 1620 248 2445
Fragments arranged by size:
2445 1620 248
KpnI G_GTAC'C
Cuts at: 1333 1333
Size: 4313
MluI A'CGCG_T
Cuts at: 3746 3746
Size: 4313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2984 3168 2984
Size: 184 4129
MscI TGG'CCA
Cuts at: 521 2074 521
Size: 1553 2760
MslI CAynn'nnrTG
Cuts at: 634 766 2302 2429 634
Size: 132 1536 127 2518
Fragments arranged by size:
2518 1536 132 127
MspA1I CmG'CkG
Cuts at: 393 1424 1738 2098 3111 3356 393
Size: 1031 314 360 1013 245 1350
Fragments arranged by size:
1350 1031 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2494 2494
Size: 4313
NarI GG'CG_CC
Cuts at: 124 1744 1992 124
Size: 1620 248 2445
Fragments arranged by size:
2445 1620 248
NcoI C'CATG_G
Cuts at: 650 905 650
Size: 255 4058
NdeI CA'TA_TG
Cuts at: 602 602
Size: 4313
NgoMIV G'CCGG_C
Cuts at: 2492 2492
Size: 4313
NspI r_CATG'y
Cuts at: 361 1366 1866 2773 361
Size: 1005 500 907 1901
Fragments arranged by size:
1901 1005 907 500
PciI A'CATG_T
Cuts at: 2769 2769
Size: 4313
PfoI T'CCnGG_A
Cuts at: 1200 1200
Size: 4313
PmeI GTTT'AAAC
Cuts at: 1312 1312
Size: 4313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3452 3484 3452
Size: 32 4281
PpuMI rG'GwC_Cy
Cuts at: 174 174
Size: 4313
PstI C_TGCA'G
Cuts at: 392 1360 2045 392
Size: 968 685 2660
Fragments arranged by size:
2660 968 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4313
PvuII CAG'CTG
Cuts at: 393 1738 2098 393
Size: 1345 360 2608
Fragments arranged by size:
2608 1345 360
RsrII CG'GwC_CG
Cuts at: 2508 2508
Size: 4313
SacI G_AGCT'C
Cuts at: 869 1327 869
Size: 458 3855
SalI G'TCGA_C
Cuts at: 893 1350 893
Size: 457 3856
SapI GCTCTTCn'nnn_
Cuts at: 2336 2546 2336
Size: 210 4103
SbfI CC_TGCA'GG
Cuts at: 1360 1360
Size: 4313
ScaI AGT'ACT
Cuts at: 3830 3830
Size: 4313
SfcI C'TryA_G
Cuts at: 33 388 1068 1356 2041 3034 3225 33
Size: 355 680 288 685 993 191 1121
Fragments arranged by size:
1121 993 685 680 355 288 191
SfoI GGC'GCC
Cuts at: 125 1745 1993 125
Size: 1620 248 2445
Fragments arranged by size:
2445 1620 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4313
SgrAI Cr'CCGG_yG
Cuts at: 400 400
Size: 4313
SmaI CCC'GGG
Cuts at: 329 491 1335 329
Size: 162 844 3307
Fragments arranged by size:
3307 844 162
SmlI C'TyrA_G
Cuts at: 1468 2383 2875 3137 1468
Size: 915 492 262 2644
Fragments arranged by size:
2644 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4313
SphI G_CATG'C
Cuts at: 1366 1866 1366
Size: 500 3813
StyI C'CwwG_G
Cuts at: 571 650 905 1447 1801 571
Size: 79 255 542 354 3083
Fragments arranged by size:
3083 542 354 255 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 484 966 1161 1896 2029 2488 2697 484
Size: 482 195 735 133 459 209 2100
Fragments arranged by size:
2100 735 482 459 209 195 133
TatI w'GTAC_w
Cuts at: 3828 3828
Size: 4313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3830 3862 3830
Size: 32 4281
Tth111I GACn'n_nGTC
Cuts at: 2110 2110
Size: 4313
XbaI T'CTAG_A
Cuts at: 1344 1344
Size: 4313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1808 1808
Size: 4313
XmaI C'CCGG_G
Cuts at: 327 489 1333 327
Size: 162 844 3307
Fragments arranged by size:
3307 844 162
XmnI GAAnn'nnTTC
Cuts at: 845 845
Size: 4313
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
BaeI BcgI BfrBI BmtI BpmI BsaXI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
ZraI