Restriction map for
pF1KB5654
(Circular) (Six-base) MAPSORT of: pF1KB5654.seq Check: 3415 from: 1 to: 4106
pF1KB5654 4106 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AatII G_ACGT'C
Cuts at: 255 255
Size: 4106
AccI GT'mk_AC
Cuts at: 905 1144 905
Size: 239 3867
Acc65I G'GTAC_C
Cuts at: 1122 1122
Size: 4106
AclI AA'CG_TT
Cuts at: 3946 3946
Size: 4106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 512 583 1931 2363 3110 3378 512
Size: 71 1348 432 747 268 1240
Fragments arranged by size:
1348 1240 747 432 268 71
AflIII A'CryG_T
Cuts at: 775 1458 2562 3539 775
Size: 683 1104 977 1342
Fragments arranged by size:
1342 1104 977 683
AgeI A'CCGG_T
Cuts at: 2494 2494
Size: 4106
AlwNI CAG_nnn'CTG
Cuts at: 606 853 1411 2978 606
Size: 247 558 1567 1734
Fragments arranged by size:
1734 1567 558 247
ApaI G_GGCC'C
Cuts at: 549 549
Size: 4106
ApaLI G'TGCA_C
Cuts at: 2876 2876
Size: 4106
ApoI r'AATT_y
Cuts at: 1110 3459 3715 1110
Size: 2349 256 1501
Fragments arranged by size:
2349 1501 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4106
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
AvaI C'yCGr_G
Cuts at: 1126 1126
Size: 4106
BamHI G'GATC_C
Cuts at: 1131 1131
Size: 4106
BanI G'GyrC_C
Cuts at: 1122 1536 1784 1819 1122
Size: 414 248 35 3409
Fragments arranged by size:
3409 414 248 35
BanII G_rGCy'C
Cuts at: 549 1120 2150 549
Size: 571 1030 2505
Fragments arranged by size:
2505 1030 571
BbeI G_GCGC'C
Cuts at: 1540 1788 1540
Size: 248 3858
BciVI GTATCCnnnnn_n'
Cuts at: 2004 2765 4106 2004
Size: 761 1341 2004
Fragments arranged by size:
2004 1341 761
BclI T'GATC_A
Cuts at: 1626 1626
Size: 4106
BglII A'GATC_T
Cuts at: 1621 3658 1621
Size: 2037 2069
BlpI GC'TnA_GC
Cuts at: 1218 1218
Size: 4106
Bme1580I G_kGCm'C
Cuts at: 549 600 1731 1824 2880 549
Size: 51 1131 93 1056 1775
Fragments arranged by size:
1775 1131 1056 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 841 1318 1399 1734 3878 841
Size: 477 81 335 2144 1069
Fragments arranged by size:
2144 1069 477 335 81
BmtI G_CTAG'C
Cuts at: 1079 1079
Size: 4106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2473 2473
Size: 4106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 489 319
Size: 170 3936
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1282 2161 2653 2951 1282
Size: 879 492 298 2437
Fragments arranged by size:
2437 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3810 3810
Size: 4106
BsaAI yAC'GTr
Cuts at: 1459 1459
Size: 4106
BsaBI GATnn'nnATC
Cuts at: 6 1620 3657 6
Size: 1614 2037 455
Fragments arranged by size:
2037 1614 455
BsaHI Gr'CG_yC
Cuts at: 252 1537 1785 2487 3983 252
Size: 1285 248 702 1496 375
Fragments arranged by size:
1496 1285 702 375 248
BsaWI w'CCGG_w
Cuts at: 1295 1816 2494 2768 2915 3326 1295
Size: 521 678 274 147 411 2075
Fragments arranged by size:
2075 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 132
Size: 4106
BseYI C'CCAG_C
Cuts at: 400 524 1004 1531 2289 2866 3362 400
Size: 124 480 527 758 577 496 1144
Fragments arranged by size:
1144 758 577 527 496 480 124
BsiEI CG_ry'CG
Cuts at: 104 1648 1694 2902 104
Size: 1544 46 1208 1308
Fragments arranged by size:
1544 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 131 142 1120 1898 2088 2880 131
Size: 11 978 778 190 792 1357
Fragments arranged by size:
1357 978 792 778 190 11
BsmI GAATG_Cn'
Cuts at: 303 320 303
Size: 17 4089
BsmBI CGTCTCn'nnnn_
Cuts at: 259 259
Size: 4106
Bsp1286I G_dGCh'C
Cuts at: 131 142 549 600 1120 1731 1824 1898 2088
Size: 11 407 51 520 611 93 74 190
Cuts at: 2088 2150 2880 131
Size: 62 730 1357
Fragments arranged by size:
1357 730 611 520 407 190 93 74
62 51 11
BspEI T'CCGG_A
Cuts at: 3326 3326
Size: 4106
BspHI T'CATG_A
Cuts at: 659 3282 659
Size: 2623 1483
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1156 1370 1672 2053 1156
Size: 214 302 381 3209
Fragments arranged by size:
3209 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2398 2452 3936 4101 50
Size: 2348 54 1484 165 55
Fragments arranged by size:
2348 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 654 2018 654
Size: 1364 2742
BsrFI r'CCGG_y
Cuts at: 2104 2285 2494 2104
Size: 181 209 3716
Fragments arranged by size:
3716 209 181
BssSI C'ACGA_G
Cuts at: 2377 2735 2377
Size: 358 3748
BstAPI GCAn_nnn'nTGC
Cuts at: 1483 1483
Size: 4106
BstBI TT'CG_AA
Cuts at: 2467 2467
Size: 4106
BstXI CCAn_nnnn'nTGG
Cuts at: 1018 1018
Size: 4106
BstYI r'GATC_y
Cuts at: 1 168 1131 1598 1621 1956 2202 3214 3300
Size: 167 963 467 23 335 246 1012 86
Cuts at: 3300 3312 3658 1
Size: 12 346 449
Fragments arranged by size:
1012 963 467 449 346 335 246 167
86 23 12
BtgI C'CryG_G
Cuts at: 731 731
Size: 4106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2056 2237 2400 2056
Size: 181 163 3762
Fragments arranged by size:
3762 181 163
BtsI GCAGTG_nn'
Cuts at: 155 1349 155
Size: 1194 2912
DraI TTT'AAA
Cuts at: 1105 1105
Size: 4106
DrdI GACnn_nn'nnGTC
Cuts at: 1812 2670 1812
Size: 858 3248
EaeI y'GGCC_r
Cuts at: 833 1691 1865 2256 2283 3628 833
Size: 858 174 391 27 1345 1311
Fragments arranged by size:
1345 1311 858 391 174 27
EagI C'GGCC_G
Cuts at: 1691 1691
Size: 4106
EarI CTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2314 2624 2770 3916 2314
Size: 310 146 1146 2504
Fragments arranged by size:
2504 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1118 1118
Size: 4106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 319 489 512 583 1931 2363 3110 3378 319
Size: 170 23 71 1348 432 747 268 1047
Fragments arranged by size:
1348 1047 747 432 268 170 71 23
EcoO109I rG'GnC_Cy
Cuts at: 545 546 1245 545
Size: 1 699 3406
Fragments arranged by size:
3406 699 1
EcoRI G'AATT_C
Cuts at: 1110 1110
Size: 4106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2125 2125
Size: 4106
FspI TGC'GCA
Cuts at: 1887 1887
Size: 4106
HaeII r_GCGC'y
Cuts at: 1540 1788 2810 3789 1540
Size: 248 1022 979 1857
Fragments arranged by size:
1857 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 242 274 800 832 1948 1978 1980 2010 242
Size: 32 526 32 1116 30 2 30 2338
Fragments arranged by size:
2338 1116 526 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1145 1145
Size: 4106
KasI G'GCGC_C
Cuts at: 1536 1784 1536
Size: 248 3858
KpnI G_GTAC'C
Cuts at: 1126 1126
Size: 4106
MluI A'CGCG_T
Cuts at: 3539 3539
Size: 4106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2777 2961 2777
Size: 184 3922
MscI TGG'CCA
Cuts at: 835 1867 835
Size: 1032 3074
MslI CAynn'nnrTG
Cuts at: 2095 2222 2095
Size: 127 3979
MspA1I CmG'CkG
Cuts at: 561 1004 1217 1531 1891 2904 3149 561
Size: 443 213 314 360 1013 245 1518
Fragments arranged by size:
1518 1013 443 360 314 245 213
NaeI GCC'GGC
Cuts at: 2287 2287
Size: 4106
NarI GG'CG_CC
Cuts at: 1537 1785 1537
Size: 248 3858
NcoI C'CATG_G
Cuts at: 731 731
Size: 4106
NgoMIV G'CCGG_C
Cuts at: 2285 2285
Size: 4106
NheI G'CTAG_C
Cuts at: 1075 1075
Size: 4106
NspI r_CATG'y
Cuts at: 779 1159 1659 2566 779
Size: 380 500 907 2319
Fragments arranged by size:
2319 907 500 380
PciI A'CATG_T
Cuts at: 775 2562 775
Size: 1787 2319
PflMI CCAn_nnn'nTGG
Cuts at: 407 606 407
Size: 199 3907
PmeI GTTT'AAAC
Cuts at: 1105 1105
Size: 4106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3245 3277 3245
Size: 32 4074
PspOMI G'GGCC_C
Cuts at: 545 545
Size: 4106
PstI C_TGCA'G
Cuts at: 150 1153 1838 150
Size: 1003 685 2418
Fragments arranged by size:
2418 1003 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4106
PvuII CAG'CTG
Cuts at: 561 1004 1531 1891 561
Size: 443 527 360 2776
Fragments arranged by size:
2776 527 443 360
RsrII CG'GwC_CG
Cuts at: 2301 2301
Size: 4106
SacI G_AGCT'C
Cuts at: 1120 1120
Size: 4106
SalI G'TCGA_C
Cuts at: 1143 1143
Size: 4106
SapI GCTCTTCn'nnn_
Cuts at: 2129 2339 2129
Size: 210 3896
SbfI CC_TGCA'GG
Cuts at: 1153 1153
Size: 4106
ScaI AGT'ACT
Cuts at: 3623 3623
Size: 4106
SexAI A'CCwGG_T
Cuts at: 605 605
Size: 4106
SfcI C'TryA_G
Cuts at: 33 146 891 1149 1834 2827 3018 33
Size: 113 745 258 685 993 191 1121
Fragments arranged by size:
1121 993 745 685 258 191 113
SfoI GGC'GCC
Cuts at: 1538 1786 1538
Size: 248 3858
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4106
SmaI CCC'GGG
Cuts at: 1128 1128
Size: 4106
SmlI C'TyrA_G
Cuts at: 1261 2176 2668 2930 1261
Size: 915 492 262 2437
Fragments arranged by size:
2437 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4106
SphI G_CATG'C
Cuts at: 1159 1659 1159
Size: 500 3606
StyI C'CwwG_G
Cuts at: 731 1240 1594 731
Size: 509 354 3243
Fragments arranged by size:
3243 509 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1689 1822 2281 2490 1689
Size: 133 459 209 3305
Fragments arranged by size:
3305 459 209 133
TatI w'GTAC_w
Cuts at: 677 785 922 945 3621 677
Size: 108 137 23 2676 1162
Fragments arranged by size:
2676 1162 137 108 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 569 601 780 812 3623 3655 569
Size: 32 179 32 2811 32 1020
Fragments arranged by size:
2811 1020 179 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 1903
Size: 4106
XbaI T'CTAG_A
Cuts at: 1137 1137
Size: 4106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1601 114
Size: 1487 2619
XmaI C'CCGG_G
Cuts at: 1126 1126
Size: 4106
ZraI GAC'GTC
Cuts at: 253 253
Size: 4106
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NspI PciI PflMI PmeI PpiI PspOMI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
Bpu10I BsaXI BsgI BsiWI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NotI NruI NsiI PacI
PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI
SacII SanDI SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI XmnI