Restriction map for
pF1KB5655
(Circular) (Six-base) MAPSORT of: pF1KB5655.seq Check: 7769 from: 1 to: 6797
pF1KB5655 6797 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 387 387
Size: 6797
AccI GT'mk_AC
Cuts at: 1747 3835 1747
Size: 2088 4709
Acc65I G'GTAC_C
Cuts at: 3813 3813
Size: 6797
AclI AA'CG_TT
Cuts at: 6637 6637
Size: 6797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 350 517 1994 2968 4622 5054 5801 6069
Size: 132 167 1477 974 1654 432 747 268
Cuts at: 6069 218
Size: 946
Fragments arranged by size:
1654 1477 974 946 747 432 268 167
132
AflII C'TTAA_G
Cuts at: 1927 3148 1927
Size: 1221 5576
AflIII A'CryG_T
Cuts at: 1525 1742 1889 1950 4149 5253 6230 1525
Size: 217 147 61 2199 1104 977 2092
Fragments arranged by size:
2199 2092 1104 977 217 147 61
AgeI A'CCGG_T
Cuts at: 829 5185 829
Size: 4356 2441
AhdI GACnn_n'nnGTC
Cuts at: 2703 2703
Size: 6797
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1159 1191 1159
Size: 32 6765
AlwNI CAG_nnn'CTG
Cuts at: 1711 4102 5669 1711
Size: 2391 1567 2839
Fragments arranged by size:
2839 2391 1567
ApaLI G'TGCA_C
Cuts at: 374 5567 374
Size: 5193 1604
ApoI r'AATT_y
Cuts at: 526 708 1216 1295 1918 1944 2172 2676 2805
Size: 182 508 79 623 26 228 504 129
Cuts at: 2805 3001 3514 3521 3801 6150 6406 526
Size: 196 513 7 280 2349 256 917
Fragments arranged by size:
2349 917 623 513 508 504 280 256
228 196 182 129 79 26 7
AseI AT'TA_AT
Cuts at: 20 1256 20
Size: 1236 5561
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6797
AvaI C'yCGr_G
Cuts at: 281 3817 281
Size: 3536 3261
BamHI G'GATC_C
Cuts at: 835 3822 835
Size: 2987 3810
BanI G'GyrC_C
Cuts at: 3813 4227 4475 4510 3813
Size: 414 248 35 6100
Fragments arranged by size:
6100 414 248 35
BanII G_rGCy'C
Cuts at: 642 960 3811 4841 642
Size: 318 2851 1030 2598
Fragments arranged by size:
2851 2598 1030 318
BbeI G_GCGC'C
Cuts at: 4231 4479 4231
Size: 248 6549
BbsI GAAGACnn'nnnn_
Cuts at: 223 331 338 1892 223
Size: 108 7 1554 5128
Fragments arranged by size:
5128 1554 108 7
BbvCI CC'TCA_GC
Cuts at: 383 658 383
Size: 275 6522
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1435 1469 1435
Size: 34 6763
BciVI GTATCCnnnnn_n'
Cuts at: 922 4695 5456 6797 922
Size: 3773 761 1341 922
Fragments arranged by size:
3773 1341 922 761
BclI T'GATC_A
Cuts at: 1083 4317 1083
Size: 3234 3563
BglII A'GATC_T
Cuts at: 850 4312 6349 850
Size: 3462 2037 1298
Fragments arranged by size:
3462 2037 1298
BlpI GC'TnA_GC
Cuts at: 1028 3909 1028
Size: 2881 3916
Bme1580I G_kGCm'C
Cuts at: 378 3271 4422 4515 5571 378
Size: 2893 1151 93 1056 1604
Fragments arranged by size:
2893 1604 1151 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 3680 4009 4090 4425 6569 3680
Size: 329 81 335 2144 3908
Fragments arranged by size:
3908 2144 335 329 81
BmtI G_CTAG'C
Cuts at: 473 473
Size: 6797
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 5164 2098
Size: 3066 3731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 296 509 614 641 1688 3353 296
Size: 213 105 27 1047 1665 3740
Fragments arranged by size:
3740 1665 1047 213 105 27
Bpu10I CC'TnA_GC
Cuts at: 383 658 383
Size: 275 6522
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1581 2086 3017 3973 4852 5344 5642 1581
Size: 505 931 956 879 492 298 2736
Fragments arranged by size:
2736 956 931 879 505 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 6501 6501
Size: 6797
BsaAI yAC'GTr
Cuts at: 3272 3431 4150 3272
Size: 159 719 5919
Fragments arranged by size:
5919 719 159
BsaBI GATnn'nnATC
Cuts at: 6 4311 6348 6
Size: 4305 2037 455
Fragments arranged by size:
4305 2037 455
BsaHI Gr'CG_yC
Cuts at: 4228 4476 5178 6674 4228
Size: 248 702 1496 4351
Fragments arranged by size:
4351 1496 702 248
BsaWI w'CCGG_w
Cuts at: 829 3986 4507 5185 5459 5606 6017 829
Size: 3157 521 678 274 147 411 1609
Fragments arranged by size:
3157 1609 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 342 372 906 936 1159 1189 1849 1879 342
Size: 30 534 30 223 30 660 30 5260
Fragments arranged by size:
5260 660 534 223 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1034 1167 3241 1034
Size: 133 2074 4590
Fragments arranged by size:
4590 2074 133
BseYI C'CCAG_C
Cuts at: 1424 4222 4980 5557 6053 1424
Size: 2798 758 577 496 2168
Fragments arranged by size:
2798 2168 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3318 3318
Size: 6797
BsiEI CG_ry'CG
Cuts at: 104 3073 4339 4385 5593 104
Size: 2969 1266 46 1208 1308
Fragments arranged by size:
2969 1308 1266 1208 46
BsiHKAI G_wGCw'C
Cuts at: 378 1581 3576 3811 4589 4779 5571 378
Size: 1203 1995 235 778 190 792 1604
Fragments arranged by size:
1995 1604 1203 792 778 235 190
BsmI GAATG_Cn'
Cuts at: 180 1604 1704 2937 180
Size: 1424 100 1233 4040
Fragments arranged by size:
4040 1424 1233 100
BsmBI CGTCTCn'nnnn_
Cuts at: 1848 1848
Size: 6797
Bsp1286I G_dGCh'C
Cuts at: 378 642 960 1581 3271 3576 3811 4422 4515
Size: 264 318 621 1690 305 235 611 93
Cuts at: 4515 4589 4779 4841 5571 378
Size: 74 190 62 730 1604
Fragments arranged by size:
1690 1604 730 621 611 318 305 264
235 190 93 74 62
BspEI T'CCGG_A
Cuts at: 6017 6017
Size: 6797
BspHI T'CATG_A
Cuts at: 5973 5973
Size: 6797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 387 2518 3847 4061 4363 4744 387
Size: 2131 1329 214 302 381 2440
Fragments arranged by size:
2440 2131 1329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5089 5143 6627 6792 50
Size: 5039 54 1484 165 55
Fragments arranged by size:
5039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1109 3124 4709 1109
Size: 2015 1585 3197
Fragments arranged by size:
3197 2015 1585
BsrFI r'CCGG_y
Cuts at: 829 4795 4976 5185 829
Size: 3966 181 209 2441
Fragments arranged by size:
3966 2441 209 181
BsrGI T'GTAC_A
Cuts at: 747 747
Size: 6797
BssSI C'ACGA_G
Cuts at: 5068 5426 5068
Size: 358 6439
BstAPI GCAn_nnn'nTGC
Cuts at: 270 3418 4174 270
Size: 3148 756 2893
Fragments arranged by size:
3148 2893 756
BstBI TT'CG_AA
Cuts at: 713 5158 713
Size: 4445 2352
BstEII G'GTnAC_C
Cuts at: 1054 1054
Size: 6797
BstYI r'GATC_y
Cuts at: 1 835 850 2048 2387 3385 3822 4289 4312
Size: 834 15 1198 339 998 437 467 23
Cuts at: 4312 4647 4893 5905 5991 6003 6349 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1198 1012 998 834 467 449 437 346
339 335 246 86 23 15 12
Bsu36I CC'TnA_GG
Cuts at: 197 3224 197
Size: 3027 3770
BtgI C'CryG_G
Cuts at: 643 3404 643
Size: 2761 4036
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4747 4928 5091 4747
Size: 181 163 6453
Fragments arranged by size:
6453 181 163
BtsI GCAGTG_nn'
Cuts at: 352 2763 4040 352
Size: 2411 1277 3109
Fragments arranged by size:
3109 2411 1277
ClaI AT'CG_AT
Cuts at: 3069 3069
Size: 6797
DraI TTT'AAA
Cuts at: 1293 1917 3796 1293
Size: 624 1879 4294
Fragments arranged by size:
4294 1879 624
DraIII CAC_nnn'GTG
Cuts at: 1497 1497
Size: 6797
DrdI GACnn_nn'nnGTC
Cuts at: 787 4503 5361 787
Size: 3716 858 2223
Fragments arranged by size:
3716 2223 858
EaeI y'GGCC_r
Cuts at: 4382 4556 4947 4974 6319 4382
Size: 174 391 27 1345 4860
Fragments arranged by size:
4860 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4382 4382
Size: 6797
EarI CTCTTCn'nnn_
Cuts at: 1121 1232 1339 1934 2792 4820 5030 1121
Size: 111 107 595 858 2028 210 2888
Fragments arranged by size:
2888 2028 858 595 210 111 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 310 5005 5315 5461 6607 310
Size: 4695 310 146 1146 500
Fragments arranged by size:
4695 1146 500 310 146
EcoICRI GAG'CTC
Cuts at: 3809 3809
Size: 6797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 296 350 509 517 614 641 1688 1994
Size: 78 54 159 8 97 27 1047 306
Cuts at: 1994 2968 3353 4622 5054 5801 6069 218
Size: 974 385 1269 432 747 268 946
Fragments arranged by size:
1269 1047 974 946 747 432 385 306
268 159 97 78 54 27 8
EcoO109I rG'GnC_Cy
Cuts at: 814 1362 3936 814
Size: 548 2574 3675
Fragments arranged by size:
3675 2574 548
EcoRI G'AATT_C
Cuts at: 3521 3801 3521
Size: 280 6517
EcoRV GAT'ATC
Cuts at: 863 863
Size: 6797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 355 1187 4816 355
Size: 832 3629 2336
Fragments arranged by size:
3629 2336 832
FspI TGC'GCA
Cuts at: 4578 4578
Size: 6797
HaeII r_GCGC'y
Cuts at: 4231 4479 5501 6480 4231
Size: 248 1022 979 4548
Fragments arranged by size:
4548 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 113 145 193 225 852 884 906 938 1729
Size: 32 48 32 627 32 22 32 791
Cuts at: 1729 1761 1818 1849 1850 1879 1881 1911 2130
Size: 32 57 31 1 29 2 30 219
Cuts at: 2130 2162 2689 2721 4639 4669 4671 4701 113
Size: 32 527 32 1918 30 2 30 2209
Fragments arranged by size:
2209 1918 791 627 527 219 57 48
32 32 32 32 32 32 32 31
30 30 30 29 22 2 2 1
HincII GTy'rAC
Cuts at: 949 3836 949
Size: 2887 3910
HindIII A'AGCT_T
Cuts at: 3343 3613 3343
Size: 270 6527
KasI G'GCGC_C
Cuts at: 4227 4475 4227
Size: 248 6549
KpnI G_GTAC'C
Cuts at: 3817 3817
Size: 6797
MluI A'CGCG_T
Cuts at: 6230 6230
Size: 6797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 564 591 2585 2804 5468 5652 564
Size: 27 1994 219 2664 184 1709
Fragments arranged by size:
2664 1994 1709 219 184 27
MscI TGG'CCA
Cuts at: 4558 4558
Size: 6797
MslI CAynn'nnrTG
Cuts at: 3431 4786 4913 3431
Size: 1355 127 5315
Fragments arranged by size:
5315 1355 127
MspA1I CmG'CkG
Cuts at: 1009 1708 3908 4222 4582 5595 5840 1009
Size: 699 2200 314 360 1013 245 1966
Fragments arranged by size:
2200 1966 1013 699 360 314 245
NaeI GCC'GGC
Cuts at: 4978 4978
Size: 6797
NarI GG'CG_CC
Cuts at: 4228 4476 4228
Size: 248 6549
NcoI C'CATG_G
Cuts at: 3404 3404
Size: 6797
NdeI CA'TA_TG
Cuts at: 2100 2100
Size: 6797
NgoMIV G'CCGG_C
Cuts at: 4976 4976
Size: 6797
NheI G'CTAG_C
Cuts at: 469 469
Size: 6797
NspI r_CATG'y
Cuts at: 1314 1529 1746 1893 1954 3850 4350 5257 1314
Size: 215 217 147 61 1896 500 907 2854
Fragments arranged by size:
2854 1896 907 500 217 215 147 61
PciI A'CATG_T
Cuts at: 1525 1742 1889 1950 5253 1525
Size: 217 147 61 3303 3069
Fragments arranged by size:
3303 3069 217 147 61
PflMI CCAn_nnn'nTGG
Cuts at: 3508 3508
Size: 6797
PfoI T'CCnGG_A
Cuts at: 486 924 486
Size: 438 6359
PmeI GTTT'AAAC
Cuts at: 3796 3796
Size: 6797
PmlI CAC'GTG
Cuts at: 3272 3272
Size: 6797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1159 1191 5936 5968 1159
Size: 32 4745 32 1988
Fragments arranged by size:
4745 1988 32 32
PpuMI rG'GwC_Cy
Cuts at: 814 1362 814
Size: 548 6249
PshAI GACnn'nnGTC
Cuts at: 2473 2473
Size: 6797
PsiI TTA'TAA
Cuts at: 2311 2311
Size: 6797
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2569 2601 3279 3311 2569
Size: 32 678 32 6055
Fragments arranged by size:
6055 678 32 32
PstI C_TGCA'G
Cuts at: 1124 3310 3844 4529 1124
Size: 2186 534 685 3392
Fragments arranged by size:
3392 2186 685 534
PvuI CG_AT'CG
Cuts at: 104 3073 104
Size: 2969 3828
PvuII CAG'CTG
Cuts at: 1009 1708 4222 4582 1009
Size: 699 2514 360 3224
Fragments arranged by size:
3224 2514 699 360
RsrII CG'GwC_CG
Cuts at: 4992 4992
Size: 6797
SacI G_AGCT'C
Cuts at: 3811 3811
Size: 6797
SalI G'TCGA_C
Cuts at: 3834 3834
Size: 6797
SapI GCTCTTCn'nnn_
Cuts at: 1339 2792 4820 5030 1339
Size: 1453 2028 210 3106
Fragments arranged by size:
3106 2028 1453 210
SbfI CC_TGCA'GG
Cuts at: 3844 3844
Size: 6797
ScaI AGT'ACT
Cuts at: 2367 6314 2367
Size: 3947 2850
SexAI A'CCwGG_T
Cuts at: 754 785 3447 754
Size: 31 2662 4104
Fragments arranged by size:
4104 2662 31
SfcI C'TryA_G
Cuts at: 33 876 1120 1322 3240 3306 3840 4525 5518
Size: 843 244 202 1918 66 534 685 993
Cuts at: 5518 5709 33
Size: 191 1121
Fragments arranged by size:
1918 1121 993 843 685 534 244 202
191 66
SfoI GGC'GCC
Cuts at: 4229 4477 4229
Size: 248 6549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6797
SmaI CCC'GGG
Cuts at: 3819 3819
Size: 6797
SmlI C'TyrA_G
Cuts at: 1596 1927 2065 3032 3148 3952 4867 5359 5621
Size: 331 138 967 116 804 915 492 262
Cuts at: 5621 1596
Size: 2772
Fragments arranged by size:
2772 967 915 804 492 331 262 138
116
SnaBI TAC'GTA
Cuts at: 3431 3431
Size: 6797
SpeI A'CTAG_T
Cuts at: 67 2152 67
Size: 2085 4712
SphI G_CATG'C
Cuts at: 3850 4350 3850
Size: 500 6297
SspI AAT'ATT
Cuts at: 1140 3636 1140
Size: 2496 4301
StyI C'CwwG_G
Cuts at: 3404 3931 4285 3404
Size: 527 354 5916
Fragments arranged by size:
5916 527 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3417 4380 4513 4972 5181 3417
Size: 963 133 459 209 5033
Fragments arranged by size:
5033 963 459 209 133
TatI w'GTAC_w
Cuts at: 747 1260 1653 1739 2223 2365 3551 6312 747
Size: 513 393 86 484 142 1186 2761 1232
Fragments arranged by size:
2761 1232 1186 513 484 393 142 86
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 901 933 3578 3610 6314 6346 901
Size: 32 2645 32 2704 32 1352
Fragments arranged by size:
2704 2645 1352 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4594 4594
Size: 6797
XbaI T'CTAG_A
Cuts at: 1581 3828 1581
Size: 2247 4550
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 774 4292 774
Size: 3518 3279
XmaI C'CCGG_G
Cuts at: 3817 3817
Size: 6797
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AleI AlfI ApaI AscI AvrII BaeI
BfrBI BglI BmgBI BsiWI BssHII BstXI BstZ17I CspCI
EcoNI FseI FspAI HpaI MfeI NotI NruI NsiI
PacI PasI PspOMI PspXI SacII SanDI SfiI SgrAI
SrfI StuI SwaI XhoI XmnI ZraI