Restriction map for
pF1KB5657
(Circular) (Six-base) MAPSORT of: pF1KB5657.seq Check: 6259 from: 1 to: 6545
pF1KB5657 6545 bp
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AccI GT'mk_AC
Cuts at: 3583 3583
Size: 6545
Acc65I G'GTAC_C
Cuts at: 1861 2051 2801 3561 1861
Size: 190 750 760 4845
Fragments arranged by size:
4845 760 750 190
AclI AA'CG_TT
Cuts at: 6385 6385
Size: 6545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2126 2390 4370 4802 5155 5817 2126
Size: 264 1980 432 353 662 2854
Fragments arranged by size:
2854 1980 662 432 353 264
AflIII A'CryG_T
Cuts at: 3897 5697 5978 3897
Size: 1800 281 4464
Fragments arranged by size:
4464 1800 281
AleI CACnn'nnGTG
Cuts at: 1184 1184
Size: 6545
AlwNI CAG_nnn'CTG
Cuts at: 927 1831 3850 5288 927
Size: 904 2019 1438 2184
Fragments arranged by size:
2184 2019 1438 904
ApaLI G'TGCA_C
Cuts at: 5383 5383
Size: 6545
ApoI r'AATT_y
Cuts at: 1455 1694 1744 1791 1908 2286 2339 2750 3208
Size: 239 50 47 117 378 53 411 458
Cuts at: 3208 3549 5898 6154 1455
Size: 341 2349 256 1846
Fragments arranged by size:
2349 1846 458 411 378 341 256 239
117 53 50 47
AseI AT'TA_AT
Cuts at: 20 20
Size: 6545
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6545
AvaI C'yCGr_G
Cuts at: 3565 3565
Size: 6545
BamHI G'GATC_C
Cuts at: 3570 3570
Size: 6545
BanI G'GyrC_C
Cuts at: 618 1861 2051 2524 2801 3561 3975 4223 4258
Size: 1243 190 473 277 760 414 248 35
Cuts at: 4258 618
Size: 2905
Fragments arranged by size:
2905 1243 760 473 414 277 248 190
35
BanII G_rGCy'C
Cuts at: 232 1308 3559 4589 232
Size: 1076 2251 1030 2188
Fragments arranged by size:
2251 2188 1076 1030
BbeI G_GCGC'C
Cuts at: 3979 4227 3979
Size: 248 6297
BbsI GAAGACnn'nnnn_
Cuts at: 658 728 1222 2982 658
Size: 70 494 1760 4221
Fragments arranged by size:
4221 1760 494 70
BciVI GTATCCnnnnn_n'
Cuts at: 2175 3025 4443 5499 6545 2175
Size: 850 1418 1056 1046 2175
Fragments arranged by size:
2175 1418 1056 1046 850
BclI T'GATC_A
Cuts at: 900 1608 2415 4065 900
Size: 708 807 1650 3380
Fragments arranged by size:
3380 1650 807 708
BfrBI ATG'CAT
Cuts at: 3459 3459
Size: 6545
BglI GCCn_nnn'nGGC
Cuts at: 227 227
Size: 6545
BglII A'GATC_T
Cuts at: 752 2216 4060 6097 752
Size: 1464 1844 2037 1200
Fragments arranged by size:
2037 1844 1464 1200
BlpI GC'TnA_GC
Cuts at: 3657 3657
Size: 6545
Bme1580I G_kGCm'C
Cuts at: 3293 4170 4263 5387 3293
Size: 877 93 1124 4451
Fragments arranged by size:
4451 1124 877 93
BmrI ACTGGGnnnn_n'
Cuts at: 1851 3757 3838 4173 6317 1851
Size: 1906 81 335 2144 2079
Fragments arranged by size:
2144 2079 1906 335 81
BmtI G_CTAG'C
Cuts at: 295 295
Size: 6545
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 330 1739 4912 330
Size: 1409 3173 1963
Fragments arranged by size:
3173 1963 1409
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1638 1996 1256
Size: 382 358 5805
Fragments arranged by size:
5805 382 358
Bpu10I CC'TnA_GC
Cuts at: 3121 3121
Size: 6545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3025 3214 3519 3721 4600 5314 5612 3025
Size: 189 305 202 879 714 298 3958
Fragments arranged by size:
3958 879 714 305 298 202 189
BsaI GGTCTCn'nnnn_
Cuts at: 2103 6249 2103
Size: 4146 2399
BsaAI yAC'GTr
Cuts at: 3898 3898
Size: 6545
BsaBI GATnn'nnATC
Cuts at: 6 2993 4059 6096 6
Size: 2987 1066 2037 455
Fragments arranged by size:
2987 2037 1066 455
BsaHI Gr'CG_yC
Cuts at: 3976 4224 4926 6422 3976
Size: 248 702 1496 4099
Fragments arranged by size:
4099 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3734 4255 4933 5344 5491 5765 3734
Size: 521 678 411 147 274 4514
Fragments arranged by size:
4514 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2035 2065 2035
Size: 30 6515
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 3171 3171
Size: 6545
BseYI C'CCAG_C
Cuts at: 854 962 3970 4728 5393 5801 854
Size: 108 3008 758 665 408 1598
Fragments arranged by size:
3008 1598 758 665 408 108
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 514 514
Size: 6545
BsiEI CG_ry'CG
Cuts at: 104 1602 4087 4133 5363 104
Size: 1498 2485 46 1230 1286
Fragments arranged by size:
2485 1498 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2232 3559 4337 4527 5387 2232
Size: 1327 778 190 860 3390
Fragments arranged by size:
3390 1327 860 778 190
BsmI GAATG_Cn'
Cuts at: 3459 3459
Size: 6545
Bsp1286I G_dGCh'C
Cuts at: 232 1308 2232 3293 3559 4170 4263 4337 4527
Size: 1076 924 1061 266 611 93 74 190
Cuts at: 4527 4589 5387 232
Size: 62 798 1390
Fragments arranged by size:
1390 1076 1061 924 798 611 266 190
93 74 62
BspHI T'CATG_A
Cuts at: 1812 3143 4977 1812
Size: 1331 1834 3380
Fragments arranged by size:
3380 1834 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3595 3809 4111 4492 3595
Size: 214 302 381 5648
Fragments arranged by size:
5648 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4837 4891 6375 6540 50
Size: 4787 54 1484 165 55
Fragments arranged by size:
4787 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2750 3291 4457 2750
Size: 541 1166 4838
Fragments arranged by size:
4838 1166 541
BsrFI r'CCGG_y
Cuts at: 4543 4724 4543
Size: 181 6364
BssSI C'ACGA_G
Cuts at: 4816 5524 4816
Size: 708 5837
BstAPI GCAn_nnn'nTGC
Cuts at: 3922 3922
Size: 6545
BstBI TT'CG_AA
Cuts at: 3048 4906 3048
Size: 1858 4687
BstXI CCAn_nnnn'nTGG
Cuts at: 400 2709 400
Size: 2309 4236
BstYI r'GATC_y
Cuts at: 1 752 1116 1123 2216 3570 4037 4060 4395
Size: 751 364 7 1093 1354 467 23 335
Cuts at: 4395 4641 4947 4959 5045 6097 1
Size: 246 306 12 86 1052 449
Fragments arranged by size:
1354 1093 1052 751 467 449 364 335
306 246 86 23 12 7
Bsu36I CC'TnA_GG
Cuts at: 239 239
Size: 6545
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 4495 4676 4839 4495
Size: 181 163 6201
Fragments arranged by size:
6201 181 163
BtsI GCAGTG_nn'
Cuts at: 147 443 1075 2905 3788 147
Size: 296 632 1830 883 2904
Fragments arranged by size:
2904 1830 883 632 296
ClaI AT'CG_AT
Cuts at: 1558 1558
Size: 6545
DraI TTT'AAA
Cuts at: 1224 1473 3410 3544 1224
Size: 249 1937 134 4225
Fragments arranged by size:
4225 1937 249 134
DraIII CAC_nnn'GTG
Cuts at: 472 472
Size: 6545
DrdI GACnn_nn'nnGTC
Cuts at: 4251 5595 4251
Size: 1344 5201
EaeI y'GGCC_r
Cuts at: 4130 4304 4695 4722 6067 4130
Size: 174 391 27 1345 4608
Fragments arranged by size:
4608 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4130 4130
Size: 6545
EarI CTCTTCn'nnn_
Cuts at: 2539 2846 4568 4778 2539
Size: 307 1722 210 4306
Fragments arranged by size:
4306 1722 307 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4753 5495 5641 6355 4753
Size: 742 146 714 4943
Fragments arranged by size:
4943 742 714 146
EcoICRI GAG'CTC
Cuts at: 3557 3557
Size: 6545
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1256 1638 1996 2126 2390 4370 4802 5155 5817
Size: 382 358 130 264 1980 432 353 662
Cuts at: 5817 1256
Size: 1984
Fragments arranged by size:
1984 1980 662 432 382 358 353 264
130
EcoO109I rG'GnC_Cy
Cuts at: 3684 3684
Size: 6545
EcoRI G'AATT_C
Cuts at: 1455 3208 3549 1455
Size: 1753 341 4451
Fragments arranged by size:
4451 1753 341
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4564 4564
Size: 6545
FspI TGC'GCA
Cuts at: 4326 4326
Size: 6545
HaeII r_GCGC'y
Cuts at: 3979 4227 5457 6228 3979
Size: 248 1230 771 4296
Fragments arranged by size:
4296 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 317 349 1108 1140 1942 1974 2074 2106 4387
Size: 32 759 32 802 32 100 32 2281
Cuts at: 4387 4417 4419 4449 317
Size: 30 2 30 2413
Fragments arranged by size:
2413 2281 802 759 100 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 441 3584 441
Size: 3143 3402
HindIII A'AGCT_T
Cuts at: 1342 1342
Size: 6545
KasI G'GCGC_C
Cuts at: 3975 4223 3975
Size: 248 6297
KpnI G_GTAC'C
Cuts at: 1865 2055 2805 3565 1865
Size: 190 750 760 4845
Fragments arranged by size:
4845 760 750 190
MluI A'CGCG_T
Cuts at: 5978 5978
Size: 6545
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 482 2679 5304 5488 482
Size: 2197 2625 184 1539
Fragments arranged by size:
2625 2197 1539 184
MscI TGG'CCA
Cuts at: 4306 4306
Size: 6545
MslI CAynn'nnrTG
Cuts at: 1184 1814 3163 3296 4534 4661 1184
Size: 630 1349 133 1238 127 3068
Fragments arranged by size:
3068 1349 1238 630 133 127
MspA1I CmG'CkG
Cuts at: 3656 3970 4330 5114 5359 3656
Size: 314 360 784 245 4842
Fragments arranged by size:
4842 784 360 314 245
NaeI GCC'GGC
Cuts at: 4726 4726
Size: 6545
NarI GG'CG_CC
Cuts at: 3976 4224 3976
Size: 248 6297
NgoMIV G'CCGG_C
Cuts at: 4724 4724
Size: 6545
NheI G'CTAG_C
Cuts at: 291 291
Size: 6545
NsiI A_TGCA'T
Cuts at: 3461 3461
Size: 6545
NspI r_CATG'y
Cuts at: 1035 3598 4098 5701 1035
Size: 2563 500 1603 1879
Fragments arranged by size:
2563 1879 1603 500
PasI CC'CwG_GG
Cuts at: 1256 1256
Size: 6545
PciI A'CATG_T
Cuts at: 5697 5697
Size: 6545
PflMI CCAn_nnn'nTGG
Cuts at: 541 541
Size: 6545
PfoI T'CCnGG_A
Cuts at: 1233 1284 1973 2882 3017 1233
Size: 51 689 909 135 4761
Fragments arranged by size:
4761 909 689 135 51
PmeI GTTT'AAAC
Cuts at: 3544 3544
Size: 6545
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4991 5023 4991
Size: 32 6513
PshAI GACnn'nnGTC
Cuts at: 191 191
Size: 6545
PstI C_TGCA'G
Cuts at: 450 3592 4277 450
Size: 3142 685 2718
Fragments arranged by size:
3142 2718 685
PvuI CG_AT'CG
Cuts at: 104 1602 104
Size: 1498 5047
PvuII CAG'CTG
Cuts at: 3970 4330 3970
Size: 360 6185
RsrII CG'GwC_CG
Cuts at: 956 4740 956
Size: 3784 2761
SacI G_AGCT'C
Cuts at: 3559 3559
Size: 6545
SalI G'TCGA_C
Cuts at: 3582 3582
Size: 6545
SapI GCTCTTCn'nnn_
Cuts at: 4568 4778 4568
Size: 210 6335
SbfI CC_TGCA'GG
Cuts at: 3592 3592
Size: 6545
ScaI AGT'ACT
Cuts at: 6062 6062
Size: 6545
SfcI C'TryA_G
Cuts at: 33 446 1161 3588 4273 5241 5432 33
Size: 413 715 2427 685 968 191 1146
Fragments arranged by size:
2427 1146 968 715 685 413 191
SfoI GGC'GCC
Cuts at: 3977 4225 3977
Size: 248 6297
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6545
SmaI CCC'GGG
Cuts at: 3567 3567
Size: 6545
SmlI C'TyrA_G
Cuts at: 3004 3193 3498 3700 4615 5329 5591 3004
Size: 189 305 202 915 714 262 3958
Fragments arranged by size:
3958 915 714 305 262 202 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6545
SphI G_CATG'C
Cuts at: 3598 4098 3598
Size: 500 6045
SspI AAT'ATT
Cuts at: 321 2706 321
Size: 2385 4160
StyI C'CwwG_G
Cuts at: 393 2047 3679 4033 393
Size: 1654 1632 354 2905
Fragments arranged by size:
2905 1654 1632 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 944 1833 2099 3090 4128 4261 4720 4929 944
Size: 889 266 991 1038 133 459 209 2560
Fragments arranged by size:
2560 1038 991 889 459 266 209 133
TatI w'GTAC_w
Cuts at: 6060 6060
Size: 6545
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2138 2170 2240 2272 2675 2707 6062 6094 2138
Size: 32 70 32 403 32 3355 32 2589
Fragments arranged by size:
3355 2589 403 70 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 331 4342 331
Size: 4011 2534
XbaI T'CTAG_A
Cuts at: 3576 3576
Size: 6545
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1362 1791 2868 4040 1362
Size: 429 1077 1172 3867
Fragments arranged by size:
3867 1172 1077 429
XmaI C'CCGG_G
Cuts at: 3565 3565
Size: 6545
XmnI GAAnn'nnTTC
Cuts at: 602 655 602
Size: 53 6492
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NheI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BsiWI
BsmBI BspEI BsrGI BssHII BstEII BstZ17I BtgI CspCI
EcoNI EcoRV FseI FspAI HpaI MfeI NcoI NdeI
NotI NruI PacI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI