Restriction map for
pF1KB5662
(Circular) (Six-base) MAPSORT of: pF1KB5662.seq Check: 9330 from: 1 to: 4229
pF1KB5662 4229 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1267 1267
Size: 4229
Acc65I G'GTAC_C
Cuts at: 1245 1245
Size: 4229
AclI AA'CG_TT
Cuts at: 4069 4069
Size: 4229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 745 1151 2054 2486 2839 3501 745
Size: 406 903 432 353 662 1473
Fragments arranged by size:
1473 903 662 432 406 353
AflIII A'CryG_T
Cuts at: 1581 3381 3662 1581
Size: 1800 281 2148
Fragments arranged by size:
2148 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 1534 2972 1534
Size: 1438 2791
ApaI G_GGCC'C
Cuts at: 371 371
Size: 4229
ApaLI G'TGCA_C
Cuts at: 3067 3067
Size: 4229
ApoI r'AATT_y
Cuts at: 992 1233 3582 3838 992
Size: 241 2349 256 1383
Fragments arranged by size:
2349 1383 256 241
AseI AT'TA_AT
Cuts at: 20 20
Size: 4229
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
AvaI C'yCGr_G
Cuts at: 185 425 1096 1138 1249 185
Size: 240 671 42 111 3165
Fragments arranged by size:
3165 671 240 111 42
BamHI G'GATC_C
Cuts at: 1254 1254
Size: 4229
BanI G'GyrC_C
Cuts at: 142 1043 1245 1659 1907 1942 142
Size: 901 202 414 248 35 2429
Fragments arranged by size:
2429 901 414 248 202 35
BanII G_rGCy'C
Cuts at: 371 859 1076 1243 2273 371
Size: 488 217 167 1030 2327
Fragments arranged by size:
2327 1030 488 217 167
BbeI G_GCGC'C
Cuts at: 146 1663 1911 146
Size: 1517 248 2464
Fragments arranged by size:
2464 1517 248
BbsI GAAGACnn'nnnn_
Cuts at: 573 735 794 573
Size: 162 59 4008
Fragments arranged by size:
4008 162 59
BbvCI CC'TCA_GC
Cuts at: 286 286
Size: 4229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4195
BciVI GTATCCnnnnn_n'
Cuts at: 2127 3183 4229 2127
Size: 1056 1046 2127
Fragments arranged by size:
2127 1056 1046
BclI T'GATC_A
Cuts at: 1749 1749
Size: 4229
BglI GCCn_nnn'nGGC
Cuts at: 296 296
Size: 4229
BglII A'GATC_T
Cuts at: 1744 3781 1744
Size: 2037 2192
BlpI GC'TnA_GC
Cuts at: 1341 1341
Size: 4229
Bme1580I G_kGCm'C
Cuts at: 250 371 981 1854 1947 3071 250
Size: 121 610 873 93 1124 1408
Fragments arranged by size:
1408 1124 873 610 121 93
BmrI ACTGGGnnnn_n'
Cuts at: 887 968 1441 1522 1857 4001 887
Size: 81 473 81 335 2144 1115
Fragments arranged by size:
2144 1115 473 335 81 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2596 2596
Size: 4229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 252
Size: 4229
Bpu10I CC'TnA_GC
Cuts at: 286 730 1019 286
Size: 444 289 3496
Fragments arranged by size:
3496 444 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1083 1405 2284 2998 3296 1083
Size: 322 879 714 298 2016
Fragments arranged by size:
2016 879 714 322 298
BsaI GGTCTCn'nnnn_
Cuts at: 3933 3933
Size: 4229
BsaAI yAC'GTr
Cuts at: 852 1582 852
Size: 730 3499
BsaBI GATnn'nnATC
Cuts at: 6 1743 3780 6
Size: 1737 2037 455
Fragments arranged by size:
2037 1737 455
BsaHI Gr'CG_yC
Cuts at: 143 191 215 1660 1908 2610 4106 143
Size: 48 24 1445 248 702 1496 266
Fragments arranged by size:
1496 1445 702 266 248 48 24
BsaWI w'CCGG_w
Cuts at: 120 889 1418 1939 2617 3028 3175 3449 120
Size: 769 529 521 678 411 147 274 900
Fragments arranged by size:
900 769 678 529 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 595
Size: 30 4199
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 769 769
Size: 4229
BseYI C'CCAG_C
Cuts at: 177 370 714 764 843 1654 2412 3077 3485
Size: 193 344 50 79 811 758 665 408
Cuts at: 3485 177
Size: 921
Fragments arranged by size:
921 811 758 665 408 344 193 79
50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1093 1093
Size: 4229
BsiEI CG_ry'CG
Cuts at: 104 1771 1817 3047 104
Size: 1667 46 1230 1286
Fragments arranged by size:
1667 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 268 1025 1243 2021 2211 3071 268
Size: 757 218 778 190 860 1426
Fragments arranged by size:
1426 860 778 757 218 190
BsmI GAATG_Cn'
Cuts at: 1013 1013
Size: 4229
BsmBI CGTCTCn'nnnn_
Cuts at: 1059 1059
Size: 4229
Bsp1286I G_dGCh'C
Cuts at: 250 268 371 859 981 1025 1076 1243 1854
Size: 18 103 488 122 44 51 167 611
Cuts at: 1854 1947 2021 2211 2273 3071 250
Size: 93 74 190 62 798 1408
Fragments arranged by size:
1408 798 611 488 190 167 122 103
93 74 62 51 44 18
BspEI T'CCGG_A
Cuts at: 889 889
Size: 4229
BspHI T'CATG_A
Cuts at: 2661 2661
Size: 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 398 1279 1493 1795 2176 398
Size: 881 214 302 381 2451
Fragments arranged by size:
2451 881 381 302 214
BsrBI CCG'CTC
Cuts at: 50 140 331 2521 2575 4059 4224 50
Size: 90 191 2190 54 1484 165 55
Fragments arranged by size:
2190 1484 191 165 90 55 54
BsrDI GCAATG_nn'
Cuts at: 2141 2141
Size: 4229
BsrFI r'CCGG_y
Cuts at: 340 2227 2408 340
Size: 1887 181 2161
Fragments arranged by size:
2161 1887 181
BssHII G'CGCG_C
Cuts at: 359 359
Size: 4229
BssSI C'ACGA_G
Cuts at: 2500 3208 2500
Size: 708 3521
BstAPI GCAn_nnn'nTGC
Cuts at: 1606 1606
Size: 4229
BstBI TT'CG_AA
Cuts at: 2590 2590
Size: 4229
BstXI CCAn_nnnn'nTGG
Cuts at: 714 714
Size: 4229
BstYI r'GATC_y
Cuts at: 1 863 1100 1254 1721 1744 2079 2325 2631
Size: 862 237 154 467 23 335 246 306
Cuts at: 2631 2643 2729 3781 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 862 467 449 335 306 246 237
154 86 23 12
BtgI C'CryG_G
Cuts at: 156 749 156
Size: 593 3636
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 363 506 2179 2360 2523 363
Size: 143 1673 181 163 2069
Fragments arranged by size:
2069 1673 181 163 143
BtsI GCAGTG_nn'
Cuts at: 690 1088 1472 690
Size: 398 384 3447
Fragments arranged by size:
3447 398 384
DraI TTT'AAA
Cuts at: 1228 1228
Size: 4229
DrdI GACnn_nn'nnGTC
Cuts at: 779 1935 3279 779
Size: 1156 1344 1729
Fragments arranged by size:
1729 1344 1156
EaeI y'GGCC_r
Cuts at: 197 239 1814 1988 2379 2406 3751 197
Size: 42 1575 174 391 27 1345 675
Fragments arranged by size:
1575 1345 675 391 174 42 27
EagI C'GGCC_G
Cuts at: 1814 1814
Size: 4229
EarI CTCTTCn'nnn_
Cuts at: 178 625 638 667 2252 2462 178
Size: 447 13 29 1585 210 1945
Fragments arranged by size:
1945 1585 447 210 29 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 359 669 2437 3179 3325 4039 359
Size: 310 1768 742 146 714 549
Fragments arranged by size:
1768 742 714 549 310 146
EcoICRI GAG'CTC
Cuts at: 1241 1241
Size: 4229
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 252 745 1151 2054 2486 2839 3501 252
Size: 493 406 903 432 353 662 980
Fragments arranged by size:
980 903 662 493 432 406 353
EcoO109I rG'GnC_Cy
Cuts at: 367 720 881 1368 367
Size: 353 161 487 3228
Fragments arranged by size:
3228 487 353 161
EcoRI G'AATT_C
Cuts at: 1233 1233
Size: 4229
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 574 759 2248 574
Size: 185 1489 2555
Fragments arranged by size:
2555 1489 185
FspI TGC'GCA
Cuts at: 2010 2010
Size: 4229
HaeII r_GCGC'y
Cuts at: 146 433 1663 1911 3141 3912 146
Size: 287 1230 248 1230 771 463
Fragments arranged by size:
1230 1230 771 463 287 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 406 438 595 627 808 840 2071 2101 2103
Size: 32 157 32 181 32 1231 30 2
Cuts at: 2103 2133 406
Size: 30 2502
Fragments arranged by size:
2502 1231 181 157 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1268 1268
Size: 4229
HindIII A'AGCT_T
Cuts at: 727 727
Size: 4229
KasI G'GCGC_C
Cuts at: 142 1659 1907 142
Size: 1517 248 2464
Fragments arranged by size:
2464 1517 248
KpnI G_GTAC'C
Cuts at: 1249 1249
Size: 4229
MluI A'CGCG_T
Cuts at: 3662 3662
Size: 4229
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 924 2988 3172 924
Size: 2064 184 1981
Fragments arranged by size:
2064 1981 184
MscI TGG'CCA
Cuts at: 1990 1990
Size: 4229
MslI CAynn'nnrTG
Cuts at: 614 2218 2345 614
Size: 1604 127 2498
Fragments arranged by size:
2498 1604 127
MspA1I CmG'CkG
Cuts at: 158 516 1340 1654 2014 2798 3043 158
Size: 358 824 314 360 784 245 1344
Fragments arranged by size:
1344 824 784 360 358 314 245
NaeI GCC'GGC
Cuts at: 342 2410 342
Size: 2068 2161
NarI GG'CG_CC
Cuts at: 143 1660 1908 143
Size: 1517 248 2464
Fragments arranged by size:
2464 1517 248
NgoMIV G'CCGG_C
Cuts at: 340 2408 340
Size: 2068 2161
NspI r_CATG'y
Cuts at: 421 609 1282 1782 3385 421
Size: 188 673 500 1603 1265
Fragments arranged by size:
1603 1265 673 500 188
PciI A'CATG_T
Cuts at: 3381 3381
Size: 4229
PfoI T'CCnGG_A
Cuts at: 352 352
Size: 4229
PmeI GTTT'AAAC
Cuts at: 1228 1228
Size: 4229
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2675 2707 2675
Size: 32 4197
PpuMI rG'GwC_Cy
Cuts at: 720 881 720
Size: 161 4068
PspOMI G'GGCC_C
Cuts at: 367 367
Size: 4229
PstI C_TGCA'G
Cuts at: 310 1276 1961 310
Size: 966 685 2578
Fragments arranged by size:
2578 966 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4229
PvuII CAG'CTG
Cuts at: 516 1654 2014 516
Size: 1138 360 2731
Fragments arranged by size:
2731 1138 360
RsrII CG'GwC_CG
Cuts at: 118 2424 118
Size: 2306 1923
SacI G_AGCT'C
Cuts at: 1243 1243
Size: 4229
SacII CC_GC'GG
Cuts at: 159 159
Size: 4229
SalI G'TCGA_C
Cuts at: 1266 1266
Size: 4229
SapI GCTCTTCn'nnn_
Cuts at: 178 667 2252 2462 178
Size: 489 1585 210 1945
Fragments arranged by size:
1945 1585 489 210
SbfI CC_TGCA'GG
Cuts at: 1276 1276
Size: 4229
ScaI AGT'ACT
Cuts at: 3746 3746
Size: 4229
SfcI C'TryA_G
Cuts at: 33 306 1272 1957 2925 3116 33
Size: 273 966 685 968 191 1146
Fragments arranged by size:
1146 968 966 685 273 191
SfoI GGC'GCC
Cuts at: 144 1661 1909 144
Size: 1517 248 2464
Fragments arranged by size:
2464 1517 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4229
SmaI CCC'GGG
Cuts at: 427 1140 1251 427
Size: 713 111 3405
Fragments arranged by size:
3405 713 111
SmlI C'TyrA_G
Cuts at: 1062 1384 2299 3013 3275 1062
Size: 322 915 714 262 2016
Fragments arranged by size:
2016 915 714 322 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4229
SphI G_CATG'C
Cuts at: 1282 1782 1282
Size: 500 3729
StuI AGG'CCT
Cuts at: 504 574 504
Size: 70 4159
StyI C'CwwG_G
Cuts at: 558 569 1363 1717 558
Size: 11 794 354 3070
Fragments arranged by size:
3070 794 354 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 106 1812 1945 2404 2613 106
Size: 1706 133 459 209 1722
Fragments arranged by size:
1722 1706 459 209 133
TatI w'GTAC_w
Cuts at: 3744 3744
Size: 4229
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3746 3778 3746
Size: 32 4197
Tth111I GACn'n_nGTC
Cuts at: 2026 2026
Size: 4229
XbaI T'CTAG_A
Cuts at: 783 1260 783
Size: 477 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 207 1724 207
Size: 1517 2712
XmaI C'CCGG_G
Cuts at: 425 1138 1249 425
Size: 713 111 3405
Fragments arranged by size:
3405 713 111
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AleI AlfI
AloI AscI AvrII BaeI BfrBI BmgBI BmtI BsiWI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI