Restriction map for
pF1KB5663
(Circular) (Six-base) MAPSORT of: pF1KB5663.seq Check: 2657 from: 1 to: 3998
pF1KB5663 3998 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1036 1036
Size: 3998
Acc65I G'GTAC_C
Cuts at: 1014 1014
Size: 3998
AclI AA'CG_TT
Cuts at: 3838 3838
Size: 3998
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 1823 2255 2608 3270 223
Size: 1600 432 353 662 951
Fragments arranged by size:
1600 951 662 432 353
AflIII A'CryG_T
Cuts at: 1350 3150 3431 1350
Size: 1800 281 1917
Fragments arranged by size:
1917 1800 281
AleI CACnn'nnGTG
Cuts at: 682 973 682
Size: 291 3707
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 104 136 104
Size: 32 3966
AlwNI CAG_nnn'CTG
Cuts at: 123 411 1303 2741 123
Size: 288 892 1438 1380
Fragments arranged by size:
1438 1380 892 288
ApaLI G'TGCA_C
Cuts at: 2836 2836
Size: 3998
ApoI r'AATT_y
Cuts at: 1002 3351 3607 1002
Size: 2349 256 1393
Fragments arranged by size:
2349 1393 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3998
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
AvaI C'yCGr_G
Cuts at: 508 1018 508
Size: 510 3488
BamHI G'GATC_C
Cuts at: 1023 1023
Size: 3998
BanI G'GyrC_C
Cuts at: 1014 1428 1676 1711 1014
Size: 414 248 35 3301
Fragments arranged by size:
3301 414 248 35
BanII G_rGCy'C
Cuts at: 386 766 1012 2042 386
Size: 380 246 1030 2342
Fragments arranged by size:
2342 1030 380 246
BbeI G_GCGC'C
Cuts at: 1432 1680 1432
Size: 248 3750
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 3998
BciVI GTATCCnnnnn_n'
Cuts at: 1896 2952 3998 1896
Size: 1056 1046 1896
Fragments arranged by size:
1896 1056 1046
BclI T'GATC_A
Cuts at: 815 1518 815
Size: 703 3295
BglII A'GATC_T
Cuts at: 1513 3550 1513
Size: 2037 1961
BlpI GC'TnA_GC
Cuts at: 1110 1110
Size: 3998
Bme1580I G_kGCm'C
Cuts at: 1623 1716 2840 1623
Size: 93 1124 2781
Fragments arranged by size:
2781 1124 93
BmgBI CAC'GTC
Cuts at: 975 984 975
Size: 9 3989
BmrI ACTGGGnnnn_n'
Cuts at: 1210 1291 1626 3770 1210
Size: 81 335 2144 1438
Fragments arranged by size:
2144 1438 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 3998
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 322 400 792 892 322
Size: 78 392 100 3428
Fragments arranged by size:
3428 392 100 78
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 535 1174 2053 2767 3065 535
Size: 639 879 714 298 1468
Fragments arranged by size:
1468 879 714 639 298
BsaI GGTCTCn'nnnn_
Cuts at: 208 445 3702 208
Size: 237 3257 504
Fragments arranged by size:
3257 504 237
BsaAI yAC'GTr
Cuts at: 1351 1351
Size: 3998
BsaBI GATnn'nnATC
Cuts at: 6 1512 3549 6
Size: 1506 2037 455
Fragments arranged by size:
2037 1506 455
BsaHI Gr'CG_yC
Cuts at: 1429 1677 2379 3875 1429
Size: 248 702 1496 1552
Fragments arranged by size:
1552 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1187 1708 2386 2797 2944 3218 1187
Size: 521 678 411 147 274 1967
Fragments arranged by size:
1967 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 104 134 586 616 104
Size: 30 452 30 3486
Fragments arranged by size:
3486 452 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 163 226 343 346 526 637 774 937 940
Size: 63 117 3 180 111 137 163 3
Cuts at: 940 949 952 955 958 163
Size: 9 3 3 3 3203
Fragments arranged by size:
3203 180 163 137 117 111 63 9
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 411 893 1423 2181 2846 3254 411
Size: 482 530 758 665 408 1155
Fragments arranged by size:
1155 758 665 530 482 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 3998
BsiEI CG_ry'CG
Cuts at: 104 183 470 1540 1586 2816 104
Size: 79 287 1070 46 1230 1286
Fragments arranged by size:
1286 1230 1070 287 79 46
BsiHKAI G_wGCw'C
Cuts at: 1012 1790 1980 2840 1012
Size: 778 190 860 2170
Fragments arranged by size:
2170 860 778 190
Bsp1286I G_dGCh'C
Cuts at: 386 766 1012 1623 1716 1790 1980 2042 2840
Size: 380 246 611 93 74 190 62 798
Cuts at: 2840 386
Size: 1544
Fragments arranged by size:
1544 798 611 380 246 190 93 74
62
BspHI T'CATG_A
Cuts at: 2430 2430
Size: 3998
BspMI ACCTGCnnnn'nnnn_
Cuts at: 226 1048 1262 1564 1945 226
Size: 822 214 302 381 2279
Fragments arranged by size:
2279 822 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2290 2344 3828 3993 50
Size: 2240 54 1484 165 55
Fragments arranged by size:
2240 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 575 1910 575
Size: 1335 2663
BsrFI r'CCGG_y
Cuts at: 1996 2177 1996
Size: 181 3817
BssHII G'CGCG_C
Cuts at: 221 221
Size: 3998
BssSI C'ACGA_G
Cuts at: 2269 2977 2269
Size: 708 3290
BstAPI GCAn_nnn'nTGC
Cuts at: 1375 1375
Size: 3998
BstBI TT'CG_AA
Cuts at: 2359 2359
Size: 3998
BstYI r'GATC_y
Cuts at: 1 1023 1490 1513 1848 2094 2400 2412 2498
Size: 1022 467 23 335 246 306 12 86
Cuts at: 2498 3550 1
Size: 1052 449
Fragments arranged by size:
1052 1022 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 757 757
Size: 3998
BtgI C'CryG_G
Cuts at: 107 710 107
Size: 603 3395
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1948 2129 2292 1948
Size: 181 163 3654
Fragments arranged by size:
3654 181 163
BtsI GCAGTG_nn'
Cuts at: 746 1241 746
Size: 495 3503
DraI TTT'AAA
Cuts at: 997 997
Size: 3998
DrdI GACnn_nn'nnGTC
Cuts at: 1704 3048 1704
Size: 1344 2654
EaeI y'GGCC_r
Cuts at: 347 467 563 734 1583 1757 2148 2175 3520
Size: 120 96 171 849 174 391 27 1345
Cuts at: 3520 347
Size: 825
Fragments arranged by size:
1345 849 825 391 174 171 120 96
27
EagI C'GGCC_G
Cuts at: 467 1583 467
Size: 1116 2882
EarI CTCTTCn'nnn_
Cuts at: 926 2021 2231 926
Size: 1095 210 2693
Fragments arranged by size:
2693 1095 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 136 223 634 692 2206 2948 3094 3808 136
Size: 87 411 58 1514 742 146 714 326
Fragments arranged by size:
1514 742 714 411 326 146 87 58
EcoICRI GAG'CTC
Cuts at: 1010 1010
Size: 3998
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 322 400 792 892 1823 2255 2608 3270
Size: 99 78 392 100 931 432 353 662
Cuts at: 3270 223
Size: 951
Fragments arranged by size:
951 931 662 432 392 353 100 99
78
EcoNI CCTnn'n_nnAGG
Cuts at: 886 886
Size: 3998
EcoO109I rG'GnC_Cy
Cuts at: 261 1137 261
Size: 876 3122
EcoRI G'AATT_C
Cuts at: 1002 1002
Size: 3998
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2017 2017
Size: 3998
FspI TGC'GCA
Cuts at: 1779 1779
Size: 3998
HaeII r_GCGC'y
Cuts at: 1432 1680 2910 3681 1432
Size: 248 1230 771 1749
Fragments arranged by size:
1749 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1840 1870 1872 1902 1840
Size: 30 2 30 3936
Fragments arranged by size:
3936 30 30 2
HincII GTy'rAC
Cuts at: 1037 1037
Size: 3998
KasI G'GCGC_C
Cuts at: 1428 1676 1428
Size: 248 3750
KpnI G_GTAC'C
Cuts at: 1018 1018
Size: 3998
MluI A'CGCG_T
Cuts at: 3431 3431
Size: 3998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2757 2941 2757
Size: 184 3814
MscI TGG'CCA
Cuts at: 349 1759 349
Size: 1410 2588
MslI CAynn'nnrTG
Cuts at: 295 424 430 682 973 1987 2114 295
Size: 129 6 252 291 1014 127 2179
Fragments arranged by size:
2179 1014 291 252 129 127 6
MspA1I CmG'CkG
Cuts at: 345 408 721 1109 1423 1783 2567 2812 345
Size: 63 313 388 314 360 784 245 1531
Fragments arranged by size:
1531 784 388 360 314 313 245 63
NaeI GCC'GGC
Cuts at: 2179 2179
Size: 3998
NarI GG'CG_CC
Cuts at: 1429 1677 1429
Size: 248 3750
NcoI C'CATG_G
Cuts at: 107 710 107
Size: 603 3395
NgoMIV G'CCGG_C
Cuts at: 2177 2177
Size: 3998
NspI r_CATG'y
Cuts at: 429 1051 1551 3154 429
Size: 622 500 1603 1273
Fragments arranged by size:
1603 1273 622 500
PciI A'CATG_T
Cuts at: 3150 3150
Size: 3998
PmeI GTTT'AAAC
Cuts at: 997 997
Size: 3998
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 104 136 584 616 2444 2476 104
Size: 32 448 32 1828 32 1626
Fragments arranged by size:
1828 1626 448 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 261 261
Size: 3998
PstI C_TGCA'G
Cuts at: 407 1045 1730 407
Size: 638 685 2675
Fragments arranged by size:
2675 685 638
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3998
PvuII CAG'CTG
Cuts at: 408 1423 1783 408
Size: 1015 360 2623
Fragments arranged by size:
2623 1015 360
RsrII CG'GwC_CG
Cuts at: 2193 2193
Size: 3998
SacI G_AGCT'C
Cuts at: 1012 1012
Size: 3998
SalI G'TCGA_C
Cuts at: 1035 1035
Size: 3998
SapI GCTCTTCn'nnn_
Cuts at: 2021 2231 2021
Size: 210 3788
SbfI CC_TGCA'GG
Cuts at: 1045 1045
Size: 3998
ScaI AGT'ACT
Cuts at: 3515 3515
Size: 3998
SfcI C'TryA_G
Cuts at: 33 403 1041 1726 2694 2885 33
Size: 370 638 685 968 191 1146
Fragments arranged by size:
1146 968 685 638 370 191
SfoI GGC'GCC
Cuts at: 1430 1678 1430
Size: 248 3750
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3998
SmaI CCC'GGG
Cuts at: 1020 1020
Size: 3998
SmlI C'TyrA_G
Cuts at: 550 1153 2068 2782 3044 550
Size: 603 915 714 262 1504
Fragments arranged by size:
1504 915 714 603 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3998
SphI G_CATG'C
Cuts at: 1051 1551 1051
Size: 500 3498
StuI AGG'CCT
Cuts at: 749 749
Size: 3998
StyI C'CwwG_G
Cuts at: 107 710 911 1132 1486 107
Size: 603 201 221 354 2619
Fragments arranged by size:
2619 603 354 221 201
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 981 1581 1714 2173 2382 981
Size: 600 133 459 209 2597
Fragments arranged by size:
2597 600 459 209 133
TatI w'GTAC_w
Cuts at: 3513 3513
Size: 3998
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 307 339 586 618 3515 3547 307
Size: 32 247 32 2897 32 758
Fragments arranged by size:
2897 758 247 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1795 1795
Size: 3998
XbaI T'CTAG_A
Cuts at: 1029 1029
Size: 3998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 297 357 1493 297
Size: 60 1136 2802
Fragments arranged by size:
2802 1136 60
XmaI C'CCGG_G
Cuts at: 1018 1018
Size: 3998
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AloI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI ApaI
AscI AvrII BaeI BbvCI BcgI BfrBI BglI BmtI
Bpu10I BsiWI BsmI BsmBI BspEI BsrGI BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
SspI SwaI XhoI XmnI ZraI