Restriction map for
pF1KB5669
(Circular) (Six-base) MAPSORT of: pF1KB5669.seq Check: 1293 from: 1 to: 4160
pF1KB5669 4160 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 698 1198 698
Size: 500 3660
Acc65I G'GTAC_C
Cuts at: 1176 1176
Size: 4160
AclI AA'CG_TT
Cuts at: 4000 4000
Size: 4160
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 273 1021 1985 2417 3164 3432 273
Size: 748 964 432 747 268 1001
Fragments arranged by size:
1001 964 748 747 432 268
AflIII A'CryG_T
Cuts at: 611 1512 2616 3593 611
Size: 901 1104 977 1178
Fragments arranged by size:
1178 1104 977 901
AgeI A'CCGG_T
Cuts at: 2548 2548
Size: 4160
AlwNI CAG_nnn'CTG
Cuts at: 450 1465 3032 450
Size: 1015 1567 1578
Fragments arranged by size:
1578 1567 1015
ApaLI G'TGCA_C
Cuts at: 2930 2930
Size: 4160
ApoI r'AATT_y
Cuts at: 232 376 565 903 1164 3513 3769 232
Size: 144 189 338 261 2349 256 623
Fragments arranged by size:
2349 623 338 261 256 189 144
AseI AT'TA_AT
Cuts at: 20 335 20
Size: 315 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
AvaI C'yCGr_G
Cuts at: 211 1180 211
Size: 969 3191
BamHI G'GATC_C
Cuts at: 114 256 1185 114
Size: 142 929 3089
Fragments arranged by size:
3089 929 142
BanI G'GyrC_C
Cuts at: 1176 1590 1838 1873 1176
Size: 414 248 35 3463
Fragments arranged by size:
3463 414 248 35
BanII G_rGCy'C
Cuts at: 1174 2204 1174
Size: 1030 3130
BbeI G_GCGC'C
Cuts at: 1594 1842 1594
Size: 248 3912
BbsI GAAGACnn'nnnn_
Cuts at: 614 614
Size: 4160
BciVI GTATCCnnnnn_n'
Cuts at: 2058 2819 4160 2058
Size: 761 1341 2058
Fragments arranged by size:
2058 1341 761
BclI T'GATC_A
Cuts at: 1680 1680
Size: 4160
BfrBI ATG'CAT
Cuts at: 1074 1074
Size: 4160
BglI GCCn_nnn'nGGC
Cuts at: 142 142
Size: 4160
BglII A'GATC_T
Cuts at: 1675 3712 1675
Size: 2037 2123
BlpI GC'TnA_GC
Cuts at: 365 1272 365
Size: 907 3253
Bme1580I G_kGCm'C
Cuts at: 1123 1785 1878 2934 1123
Size: 662 93 1056 2349
Fragments arranged by size:
2349 1056 662 93
BmrI ACTGGGnnnn_n'
Cuts at: 200 1372 1453 1788 3932 200
Size: 1172 81 335 2144 428
Fragments arranged by size:
2144 1172 428 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 258 184
Size: 74 4086
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1336 2215 2707 3005 1336
Size: 879 492 298 2491
Fragments arranged by size:
2491 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3864 3864
Size: 4160
BsaAI yAC'GTr
Cuts at: 1513 1513
Size: 4160
BsaBI GATnn'nnATC
Cuts at: 6 1674 3711 6
Size: 1668 2037 455
Fragments arranged by size:
2037 1668 455
BsaHI Gr'CG_yC
Cuts at: 1591 1839 2541 4037 1591
Size: 248 702 1496 1714
Fragments arranged by size:
1714 1496 702 248
BsaWI w'CCGG_w
Cuts at: 540 570 1349 1870 2548 2822 2969 3380 540
Size: 30 779 521 678 274 147 411 1320
Fragments arranged by size:
1320 779 678 521 411 274 147 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 205 410 205
Size: 205 3955
BseYI C'CCAG_C
Cuts at: 1585 2343 2920 3416 1585
Size: 758 577 496 2329
Fragments arranged by size:
2329 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 178 1702 1748 2956 104
Size: 74 1524 46 1208 1308
Fragments arranged by size:
1524 1308 1208 74 46
BsiHKAI G_wGCw'C
Cuts at: 1174 1952 2142 2934 1174
Size: 778 190 792 2400
Fragments arranged by size:
2400 792 778 190
BsmI GAATG_Cn'
Cuts at: 1037 1037
Size: 4160
Bsp1286I G_dGCh'C
Cuts at: 1123 1174 1785 1878 1952 2142 2204 2934 1123
Size: 51 611 93 74 190 62 730 2349
Fragments arranged by size:
2349 730 611 190 93 74 62 51
BspEI T'CCGG_A
Cuts at: 3380 3380
Size: 4160
BspHI T'CATG_A
Cuts at: 3336 3336
Size: 4160
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1210 1424 1726 2107 1210
Size: 214 302 381 3263
Fragments arranged by size:
3263 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2452 2506 3990 4155 50
Size: 2402 54 1484 165 55
Fragments arranged by size:
2402 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2072 2072
Size: 4160
BsrFI r'CCGG_y
Cuts at: 136 2158 2339 2548 136
Size: 2022 181 209 1748
Fragments arranged by size:
2022 1748 209 181
BsrGI T'GTAC_A
Cuts at: 549 549
Size: 4160
BssSI C'ACGA_G
Cuts at: 2431 2789 2431
Size: 358 3802
BstAPI GCAn_nnn'nTGC
Cuts at: 1537 1537
Size: 4160
BstBI TT'CG_AA
Cuts at: 1029 2521 1029
Size: 1492 2668
BstYI r'GATC_y
Cuts at: 1 114 256 1185 1652 1675 2010 2256 3268
Size: 113 142 929 467 23 335 246 1012
Cuts at: 3268 3354 3366 3712 1
Size: 86 12 346 449
Fragments arranged by size:
1012 929 467 449 346 335 246 142
113 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 719 719
Size: 4160
BtgI C'CryG_G
Cuts at: 107 146 172 107
Size: 39 26 4095
Fragments arranged by size:
4095 39 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2110 2291 2454 2110
Size: 181 163 3816
Fragments arranged by size:
3816 181 163
BtsI GCAGTG_nn'
Cuts at: 1403 1403
Size: 4160
DraI TTT'AAA
Cuts at: 1159 1159
Size: 4160
DrdI GACnn_nn'nnGTC
Cuts at: 1866 2724 1866
Size: 858 3302
EaeI y'GGCC_r
Cuts at: 175 240 1745 1919 2310 2337 3682 175
Size: 65 1505 174 391 27 1345 653
Fragments arranged by size:
1505 1345 653 391 174 65 27
EagI C'GGCC_G
Cuts at: 175 1745 175
Size: 1570 2590
EarI CTCTTCn'nnn_
Cuts at: 412 1107 2183 2393 412
Size: 695 1076 210 2179
Fragments arranged by size:
2179 1076 695 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2368 2678 2824 3970 2368
Size: 310 146 1146 2558
Fragments arranged by size:
2558 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1172 1172
Size: 4160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 258 273 1021 1985 2417 3164 3432 184
Size: 74 15 748 964 432 747 268 912
Fragments arranged by size:
964 912 748 747 432 268 74 15
EcoO109I rG'GnC_Cy
Cuts at: 1299 1299
Size: 4160
EcoRI G'AATT_C
Cuts at: 376 1164 376
Size: 788 3372
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2179 2179
Size: 4160
FspI TGC'GCA
Cuts at: 1941 1941
Size: 4160
HaeII r_GCGC'y
Cuts at: 1594 1842 2864 3843 1594
Size: 248 1022 979 1911
Fragments arranged by size:
1911 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2002 2032 2034 2064 2002
Size: 30 2 30 4098
Fragments arranged by size:
4098 30 30 2
HincII GTy'rAC
Cuts at: 838 1199 838
Size: 361 3799
HindIII A'AGCT_T
Cuts at: 367 382 367
Size: 15 4145
KasI G'GCGC_C
Cuts at: 1590 1838 1590
Size: 248 3912
KpnI G_GTAC'C
Cuts at: 1180 1180
Size: 4160
MluI A'CGCG_T
Cuts at: 3593 3593
Size: 4160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2831 3015 2831
Size: 184 3976
MscI TGG'CCA
Cuts at: 242 1921 242
Size: 1679 2481
MslI CAynn'nnrTG
Cuts at: 2149 2276 2149
Size: 127 4033
MspA1I CmG'CkG
Cuts at: 174 651 1271 1585 1945 2958 3203 174
Size: 477 620 314 360 1013 245 1131
Fragments arranged by size:
1131 1013 620 477 360 314 245
NaeI GCC'GGC
Cuts at: 138 2341 138
Size: 2203 1957
NarI GG'CG_CC
Cuts at: 1591 1839 1591
Size: 248 3912
NcoI C'CATG_G
Cuts at: 107 107
Size: 4160
NdeI CA'TA_TG
Cuts at: 897 897
Size: 4160
NgoMIV G'CCGG_C
Cuts at: 136 2339 136
Size: 2203 1957
NsiI A_TGCA'T
Cuts at: 1076 1076
Size: 4160
NspI r_CATG'y
Cuts at: 1078 1213 1713 2620 1078
Size: 135 500 907 2618
Fragments arranged by size:
2618 907 500 135
PciI A'CATG_T
Cuts at: 2616 2616
Size: 4160
PmeI GTTT'AAAC
Cuts at: 1159 1159
Size: 4160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 4128
PsiI TTA'TAA
Cuts at: 437 437
Size: 4160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 392 424 392
Size: 32 4128
PstI C_TGCA'G
Cuts at: 1207 1892 1207
Size: 685 3475
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4160
PvuII CAG'CTG
Cuts at: 651 1585 1945 651
Size: 934 360 2866
Fragments arranged by size:
2866 934 360
RsrII CG'GwC_CG
Cuts at: 2355 2355
Size: 4160
SacI G_AGCT'C
Cuts at: 1174 1174
Size: 4160
SacII CC_GC'GG
Cuts at: 175 175
Size: 4160
SalI G'TCGA_C
Cuts at: 1197 1197
Size: 4160
SapI GCTCTTCn'nnn_
Cuts at: 2183 2393 2183
Size: 210 3950
SbfI CC_TGCA'GG
Cuts at: 1207 1207
Size: 4160
ScaI AGT'ACT
Cuts at: 3677 3677
Size: 4160
SfcI C'TryA_G
Cuts at: 33 1203 1888 2881 3072 33
Size: 1170 685 993 191 1121
Fragments arranged by size:
1170 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1592 1840 1592
Size: 248 3912
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4160
SmaI CCC'GGG
Cuts at: 1182 1182
Size: 4160
SmlI C'TyrA_G
Cuts at: 1315 2230 2722 2984 1315
Size: 915 492 262 2491
Fragments arranged by size:
2491 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4160
SphI G_CATG'C
Cuts at: 1078 1213 1713 1078
Size: 135 500 3525
Fragments arranged by size:
3525 500 135
SspI AAT'ATT
Cuts at: 885 885
Size: 4160
StyI C'CwwG_G
Cuts at: 107 1294 1648 107
Size: 1187 354 2619
Fragments arranged by size:
2619 1187 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1034 1743 1876 2335 2544 1034
Size: 709 133 459 209 2650
Fragments arranged by size:
2650 709 459 209 133
TatI w'GTAC_w
Cuts at: 549 3675 549
Size: 3126 1034
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4128
Tth111I GACn'n_nGTC
Cuts at: 1957 1957
Size: 4160
XbaI T'CTAG_A
Cuts at: 1191 1191
Size: 4160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1655 1655
Size: 4160
XmaI C'CCGG_G
Cuts at: 1180 1180
Size: 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PmeI PpiI PsiI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NheI NotI NruI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI SanDI SexAI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI XmnI
ZraI