Restriction map for
pF1KB5676
(Circular) (Six-base) MAPSORT of: pF1KB5676.seq Check: 7963 from: 1 to: 5531
pF1KB5676 5531 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 506 2492 2569 506
Size: 1986 77 3468
Fragments arranged by size:
3468 1986 77
Acc65I G'GTAC_C
Cuts at: 2547 2547
Size: 5531
AclI AA'CG_TT
Cuts at: 5371 5371
Size: 5531
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 1639 2377 2403 2414 3356 3788 4141 4803
Size: 405 738 26 11 942 432 353 662
Cuts at: 4803 1234
Size: 1962
Fragments arranged by size:
1962 942 738 662 432 405 353 26
11
AflII C'TTAA_G
Cuts at: 511 511
Size: 5531
AflIII A'CryG_T
Cuts at: 1125 2883 4683 4964 1125
Size: 1758 1800 281 1692
Fragments arranged by size:
1800 1758 1692 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1346 1365 1346
Size: 19 5512
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2110 2142 2110
Size: 32 5499
AlwNI CAG_nnn'CTG
Cuts at: 1360 1581 1920 2293 2836 4274 1360
Size: 221 339 373 543 1438 2617
Fragments arranged by size:
2617 1438 543 373 339 221
ApaLI G'TGCA_C
Cuts at: 4369 4369
Size: 5531
ApoI r'AATT_y
Cuts at: 1217 1612 2535 4884 5140 1217
Size: 395 923 2349 256 1608
Fragments arranged by size:
2349 1608 923 395 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5531
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
AvaI C'yCGr_G
Cuts at: 872 2551 872
Size: 1679 3852
AvrII C'CTAG_G
Cuts at: 393 393
Size: 5531
BamHI G'GATC_C
Cuts at: 2556 2556
Size: 5531
BanI G'GyrC_C
Cuts at: 1518 2547 2961 3209 3244 1518
Size: 1029 414 248 35 3805
Fragments arranged by size:
3805 1029 414 248 35
BanII G_rGCy'C
Cuts at: 2545 3575 2545
Size: 1030 4501
BbeI G_GCGC'C
Cuts at: 2965 3213 2965
Size: 248 5283
BbsI GAAGACnn'nnnn_
Cuts at: 1544 2507 1544
Size: 963 4568
BciVI GTATCCnnnnn_n'
Cuts at: 457 1601 3429 4485 5531 457
Size: 1144 1828 1056 1046 457
Fragments arranged by size:
1828 1144 1056 1046 457
BclI T'GATC_A
Cuts at: 911 2025 3051 911
Size: 1114 1026 3391
Fragments arranged by size:
3391 1114 1026
BglII A'GATC_T
Cuts at: 3046 5083 3046
Size: 2037 3494
BlpI GC'TnA_GC
Cuts at: 2643 2643
Size: 5531
Bme1580I G_kGCm'C
Cuts at: 1125 3156 3249 4373 1125
Size: 2031 93 1124 2283
Fragments arranged by size:
2283 2031 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 2095 2450 2743 2824 3159 5303 2095
Size: 355 293 81 335 2144 2323
Fragments arranged by size:
2323 2144 355 335 293 81
BmtI G_CTAG'C
Cuts at: 748 748
Size: 5531
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3898 3898
Size: 5531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 623 914 2177 137
Size: 486 291 1263 3491
Fragments arranged by size:
3491 1263 486 291
Bpu10I CC'TnA_GC
Cuts at: 1742 1742
Size: 5531
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1494 2062 2707 3586 4300 4598 1494
Size: 568 645 879 714 298 2427
Fragments arranged by size:
2427 879 714 645 568 298
BsaI GGTCTCn'nnnn_
Cuts at: 1405 5235 1405
Size: 3830 1701
BsaAI yAC'GTr
Cuts at: 2884 2884
Size: 5531
BsaBI GATnn'nnATC
Cuts at: 6 3045 5082 6
Size: 3039 2037 455
Fragments arranged by size:
3039 2037 455
BsaHI Gr'CG_yC
Cuts at: 2962 3210 3912 5408 2962
Size: 248 702 1496 3085
Fragments arranged by size:
3085 1496 702 248
BsaWI w'CCGG_w
Cuts at: 545 1463 2720 3241 3919 4330 4477 4751 545
Size: 918 1257 521 678 411 147 274 1325
Fragments arranged by size:
1325 1257 918 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1711 1741 1711
Size: 30 5501
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 994 994
Size: 5531
BseYI C'CCAG_C
Cuts at: 233 1170 2956 3714 4379 4787 233
Size: 937 1786 758 665 408 977
Fragments arranged by size:
1786 977 937 758 665 408
BsiEI CG_ry'CG
Cuts at: 104 3073 3119 4349 104
Size: 2969 46 1230 1286
Fragments arranged by size:
2969 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 1354 1604 2545 3323 3513 4373 1354
Size: 250 941 778 190 860 2512
Fragments arranged by size:
2512 941 860 778 250 190
BsmI GAATG_Cn'
Cuts at: 999 1714 999
Size: 715 4816
BsmBI CGTCTCn'nnnn_
Cuts at: 291 291
Size: 5531
Bsp1286I G_dGCh'C
Cuts at: 1125 1354 1604 2545 3156 3249 3323 3513 3575
Size: 229 250 941 611 93 74 190 62
Cuts at: 3575 4373 1125
Size: 798 2283
Fragments arranged by size:
2283 941 798 611 250 229 190 93
74 62
BspEI T'CCGG_A
Cuts at: 545 545
Size: 5531
BspHI T'CATG_A
Cuts at: 929 1430 3963 929
Size: 501 2533 2497
Fragments arranged by size:
2533 2497 501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2096 2581 2795 3097 3478 2096
Size: 485 214 302 381 4149
Fragments arranged by size:
4149 485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1071 3823 3877 5361 5526 50
Size: 1021 2752 54 1484 165 55
Fragments arranged by size:
2752 1484 1021 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1697 3443 1697
Size: 1746 3785
BsrFI r'CCGG_y
Cuts at: 1979 3529 3710 1979
Size: 1550 181 3800
Fragments arranged by size:
3800 1550 181
BssHII G'CGCG_C
Cuts at: 2335 2335
Size: 5531
BssSI C'ACGA_G
Cuts at: 1862 3802 4510 1862
Size: 1940 708 2883
Fragments arranged by size:
2883 1940 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2908 2908
Size: 5531
BstBI TT'CG_AA
Cuts at: 3892 3892
Size: 5531
BstEII G'GTnAC_C
Cuts at: 1527 1527
Size: 5531
BstXI CCAn_nnnn'nTGG
Cuts at: 2115 2115
Size: 5531
BstYI r'GATC_y
Cuts at: 1 612 1253 1389 1916 2297 2556 3023 3046
Size: 611 641 136 527 381 259 467 23
Cuts at: 3046 3381 3627 3933 3945 4031 5083 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 641 611 527 467 449 381 335
306 259 246 136 86 23 12
BstZ17I GTA'TAC
Cuts at: 507 2493 507
Size: 1986 3545
BtgI C'CryG_G
Cuts at: 107 570 1508 2264 107
Size: 463 938 756 3374
Fragments arranged by size:
3374 938 756 463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1017 3481 3662 3825 1017
Size: 2464 181 163 2723
Fragments arranged by size:
2723 2464 181 163
BtsI GCAGTG_nn'
Cuts at: 654 1495 2472 2493 2774 654
Size: 841 977 21 281 3411
Fragments arranged by size:
3411 977 841 281 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1845 1880 1845
Size: 35 5496
DraI TTT'AAA
Cuts at: 2530 2530
Size: 5531
DraIII CAC_nnn'GTG
Cuts at: 1757 1757
Size: 5531
DrdI GACnn_nn'nnGTC
Cuts at: 2018 3237 4581 2018
Size: 1219 1344 2968
Fragments arranged by size:
2968 1344 1219
EaeI y'GGCC_r
Cuts at: 1688 2016 2198 3116 3290 3681 3708 5053 1688
Size: 328 182 918 174 391 27 1345 2166
Fragments arranged by size:
2166 1345 918 391 328 182 174 27
EagI C'GGCC_G
Cuts at: 3116 3116
Size: 5531
EarI CTCTTCn'nnn_
Cuts at: 289 502 686 2270 3554 3764 289
Size: 213 184 1584 1284 210 2056
Fragments arranged by size:
2056 1584 1284 213 210 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3739 4481 4627 5341 3739
Size: 742 146 714 3929
Fragments arranged by size:
3929 742 714 146
EcoICRI GAG'CTC
Cuts at: 2543 2543
Size: 5531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 137 623 914 1234 1639 2177 2377 2403 2414
Size: 486 291 320 405 538 200 26 11
Cuts at: 2414 3356 3788 4141 4803 137
Size: 942 432 353 662 865
Fragments arranged by size:
942 865 662 538 486 432 405 353
320 291 200 26 11
EcoNI CCTnn'n_nnAGG
Cuts at: 796 821 851 1297 796
Size: 25 30 446 5030
Fragments arranged by size:
5030 446 30 25
EcoO109I rG'GnC_Cy
Cuts at: 1548 1738 2670 1548
Size: 190 932 4409
Fragments arranged by size:
4409 932 190
EcoRI G'AATT_C
Cuts at: 1217 2535 1217
Size: 1318 4213
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 537 3550 537
Size: 3013 2518
FspI TGC'GCA
Cuts at: 2092 3312 2092
Size: 1220 4311
HaeII r_GCGC'y
Cuts at: 1973 2965 3213 4443 5214 1973
Size: 992 248 1230 771 2290
Fragments arranged by size:
2290 1230 992 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 127 159 679 711 903 935 1924 1956 2044
Size: 32 520 32 192 32 989 32 88
Cuts at: 2044 2076 3373 3403 3405 3435 127
Size: 32 1297 30 2 30 2223
Fragments arranged by size:
2223 1297 989 520 192 88 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1094 2570 1094
Size: 1476 4055
KasI G'GCGC_C
Cuts at: 2961 3209 2961
Size: 248 5283
KpnI G_GTAC'C
Cuts at: 2551 2551
Size: 5531
MfeI C'AATT_G
Cuts at: 803 803
Size: 5531
MluI A'CGCG_T
Cuts at: 4964 4964
Size: 5531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4290 4474 4290
Size: 184 5347
MscI TGG'CCA
Cuts at: 1690 2200 3292 1690
Size: 510 1092 3929
Fragments arranged by size:
3929 1092 510
MslI CAynn'nnrTG
Cuts at: 1322 1328 3520 3647 1322
Size: 6 2192 127 3206
Fragments arranged by size:
3206 2192 127 6
MspA1I CmG'CkG
Cuts at: 2642 2956 3316 4100 4345 2642
Size: 314 360 784 245 3828
Fragments arranged by size:
3828 784 360 314 245
NaeI GCC'GGC
Cuts at: 3712 3712
Size: 5531
NarI GG'CG_CC
Cuts at: 2962 3210 2962
Size: 248 5283
NcoI C'CATG_G
Cuts at: 107 2264 107
Size: 2157 3374
NgoMIV G'CCGG_C
Cuts at: 3710 3710
Size: 5531
NheI G'CTAG_C
Cuts at: 744 744
Size: 5531
NspI r_CATG'y
Cuts at: 1129 1327 1941 2584 3084 4687 1129
Size: 198 614 643 500 1603 1973
Fragments arranged by size:
1973 1603 643 614 500 198
PciI A'CATG_T
Cuts at: 1125 4683 1125
Size: 3558 1973
PfoI T'CCnGG_A
Cuts at: 524 846 524
Size: 322 5209
PmeI GTTT'AAAC
Cuts at: 2530 2530
Size: 5531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3977 4009 3977
Size: 32 5499
PpuMI rG'GwC_Cy
Cuts at: 1738 1738
Size: 5531
PspXI vC'TCGA_Gb
Cuts at: 872 872
Size: 5531
PstI C_TGCA'G
Cuts at: 736 2578 3263 736
Size: 1842 685 3004
Fragments arranged by size:
3004 1842 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5531
PvuII CAG'CTG
Cuts at: 2956 3316 2956
Size: 360 5171
RsrII CG'GwC_CG
Cuts at: 3726 3726
Size: 5531
SacI G_AGCT'C
Cuts at: 2545 2545
Size: 5531
SalI G'TCGA_C
Cuts at: 2568 2568
Size: 5531
SapI GCTCTTCn'nnn_
Cuts at: 3554 3764 3554
Size: 210 5321
SbfI CC_TGCA'GG
Cuts at: 2578 2578
Size: 5531
ScaI AGT'ACT
Cuts at: 5048 5048
Size: 5531
SfcI C'TryA_G
Cuts at: 33 732 878 1229 1573 2574 3259 4227 4418
Size: 699 146 351 344 1001 685 968 191
Cuts at: 4418 33
Size: 1146
Fragments arranged by size:
1146 1001 968 699 685 351 344 191
146
SfoI GGC'GCC
Cuts at: 2963 3211 2963
Size: 248 5283
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5531
SmaI CCC'GGG
Cuts at: 2553 2553
Size: 5531
SmlI C'TyrA_G
Cuts at: 511 872 1473 2077 2686 3601 4315 4577 511
Size: 361 601 604 609 915 714 262 1465
Fragments arranged by size:
1465 915 714 609 604 601 361 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5531
SphI G_CATG'C
Cuts at: 2584 3084 2584
Size: 500 5031
StyI C'CwwG_G
Cuts at: 107 393 1640 1856 2108 2264 2665 3019 107
Size: 286 1247 216 252 156 401 354 2619
Fragments arranged by size:
2619 1247 401 354 286 252 216 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1318 1619 1916 3114 3247 3706 3915 1318
Size: 301 297 1198 133 459 209 2934
Fragments arranged by size:
2934 1198 459 301 297 209 133
TatI w'GTAC_w
Cuts at: 5046 5046
Size: 5531
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2427 2459 5048 5080 2427
Size: 32 2589 32 2878
Fragments arranged by size:
2878 2589 32 32
Tth111I GACn'n_nGTC
Cuts at: 1903 3328 1903
Size: 1425 4106
XbaI T'CTAG_A
Cuts at: 2562 2562
Size: 5531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1529 2446 3026 1529
Size: 917 580 4034
Fragments arranged by size:
4034 917 580
XhoI C'TCGA_G
Cuts at: 872 872
Size: 5531
XmaI C'CCGG_G
Cuts at: 2551 2551
Size: 5531
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PciI PfoI PmeI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AleI ApaI AscI
BaeI BbvCI BcgI BfrBI BglI BmgBI BsgI BsiWI
BsrGI Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI
NdeI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI