Restriction map for
pF1KB5679
(Circular) (Six-base) MAPSORT of: pF1KB5679.seq Check: 3922 from: 1 to: 5993
pF1KB5679 5993 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AatII G_ACGT'C
Cuts at: 1150 2703 1150
Size: 1553 4440
AccI GT'mk_AC
Cuts at: 3031 3031
Size: 5993
Acc65I G'GTAC_C
Cuts at: 1856 3009 1856
Size: 1153 4840
AclI AA'CG_TT
Cuts at: 5833 5833
Size: 5993
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1039 2272 2641 3818 4250 4997 5265 457
Size: 582 1233 369 1177 432 747 268 1185
Fragments arranged by size:
1233 1185 1177 747 582 432 369 268
AfeI AGC'GCT
Cuts at: 1801 1801
Size: 5993
AflII C'TTAA_G
Cuts at: 1279 1279
Size: 5993
AflIII A'CryG_T
Cuts at: 3345 4449 5426 3345
Size: 1104 977 3912
Fragments arranged by size:
3912 1104 977
AgeI A'CCGG_T
Cuts at: 4381 4381
Size: 5993
AlwNI CAG_nnn'CTG
Cuts at: 3298 4865 3298
Size: 1567 4426
ApaI G_GGCC'C
Cuts at: 1845 1845
Size: 5993
ApaLI G'TGCA_C
Cuts at: 4763 4763
Size: 5993
ApoI r'AATT_y
Cuts at: 683 1398 1525 1972 2576 2764 2997 5346 5602
Size: 715 127 447 604 188 233 2349 256
Cuts at: 5602 683
Size: 1074
Fragments arranged by size:
2349 1074 715 604 447 256 233 188
127
AseI AT'TA_AT
Cuts at: 20 20
Size: 5993
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5993
AvaI C'yCGr_G
Cuts at: 3013 3013
Size: 5993
BamHI G'GATC_C
Cuts at: 252 1656 3018 252
Size: 1404 1362 3227
Fragments arranged by size:
3227 1404 1362
BanI G'GyrC_C
Cuts at: 1856 3009 3423 3671 3706 1856
Size: 1153 414 248 35 4143
Fragments arranged by size:
4143 1153 414 248 35
BanII G_rGCy'C
Cuts at: 1845 2338 2779 3007 4037 1845
Size: 493 441 228 1030 3801
Fragments arranged by size:
3801 1030 493 441 228
BbeI G_GCGC'C
Cuts at: 3427 3675 3427
Size: 248 5745
BbsI GAAGACnn'nnnn_
Cuts at: 1832 1832
Size: 5993
BciVI GTATCCnnnnn_n'
Cuts at: 3891 4652 5993 3891
Size: 761 1341 3891
Fragments arranged by size:
3891 1341 761
BclI T'GATC_A
Cuts at: 2795 3513 2795
Size: 718 5275
BfrBI ATG'CAT
Cuts at: 206 970 206
Size: 764 5229
BglII A'GATC_T
Cuts at: 447 3508 5545 447
Size: 3061 2037 895
Fragments arranged by size:
3061 2037 895
BlpI GC'TnA_GC
Cuts at: 3105 3105
Size: 5993
Bme1580I G_kGCm'C
Cuts at: 1845 3618 3711 4767 1845
Size: 1773 93 1056 3071
Fragments arranged by size:
3071 1773 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 262 311 417 2427 3205 3286 3621 5765 262
Size: 49 106 2010 778 81 335 2144 490
Fragments arranged by size:
2144 2010 778 490 335 106 81 49
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4360 4360
Size: 5993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1127 1720 1792 2343 2962 1127
Size: 593 72 551 619 4158
Fragments arranged by size:
4158 619 593 551 72
Bpu10I CC'TnA_GC
Cuts at: 307 307
Size: 5993
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1486 2257 2861 2890 3169 4048 4540 4838 1486
Size: 771 604 29 279 879 492 298 2641
Fragments arranged by size:
2641 879 771 604 492 298 279 29
BsaI GGTCTCn'nnnn_
Cuts at: 5697 5697
Size: 5993
BsaAI yAC'GTr
Cuts at: 3346 3346
Size: 5993
BsaBI GATnn'nnATC
Cuts at: 6 2797 3507 5544 6
Size: 2791 710 2037 455
Fragments arranged by size:
2791 2037 710 455
BsaHI Gr'CG_yC
Cuts at: 1147 2700 3424 3672 4374 5870 1147
Size: 1553 724 248 702 1496 1270
Fragments arranged by size:
1553 1496 1270 724 702 248
BsaWI w'CCGG_w
Cuts at: 3182 3703 4381 4655 4802 5213 3182
Size: 521 678 274 147 411 3962
Fragments arranged by size:
3962 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1534 1534
Size: 5993
BseYI C'CCAG_C
Cuts at: 3418 4176 4753 5249 3418
Size: 758 577 496 4162
Fragments arranged by size:
4162 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 2141
Size: 5993
BsiEI CG_ry'CG
Cuts at: 104 3535 3581 4789 104
Size: 3431 46 1208 1308
Fragments arranged by size:
3431 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2505 3007 3785 3975 4767 2505
Size: 502 778 190 792 3731
Fragments arranged by size:
3731 792 778 502 190
BsmI GAATG_Cn'
Cuts at: 468 468
Size: 5993
BsmBI CGTCTCn'nnnn_
Cuts at: 1138 1138
Size: 5993
Bsp1286I G_dGCh'C
Cuts at: 1845 2338 2505 2779 3007 3618 3711 3785 3975
Size: 493 167 274 228 611 93 74 190
Cuts at: 3975 4037 4767 1845
Size: 62 730 3071
Fragments arranged by size:
3071 730 611 493 274 228 190 167
93 74 62
BspEI T'CCGG_A
Cuts at: 5213 5213
Size: 5993
BspHI T'CATG_A
Cuts at: 540 2097 5169 540
Size: 1557 3072 1364
Fragments arranged by size:
3072 1557 1364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3043 3257 3559 3940 3043
Size: 214 302 381 5096
Fragments arranged by size:
5096 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2360 4285 4339 5823 5988 50
Size: 2310 1925 54 1484 165 55
Fragments arranged by size:
2310 1925 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 131 420 3905 131
Size: 289 3485 2219
Fragments arranged by size:
3485 2219 289
BsrFI r'CCGG_y
Cuts at: 3991 4172 4381 3991
Size: 181 209 5603
Fragments arranged by size:
5603 209 181
BssSI C'ACGA_G
Cuts at: 1887 4264 4622 1887
Size: 2377 358 3258
Fragments arranged by size:
3258 2377 358
BstAPI GCAn_nnn'nTGC
Cuts at: 3370 3370
Size: 5993
BstBI TT'CG_AA
Cuts at: 4354 4354
Size: 5993
BstEII G'GTnAC_C
Cuts at: 1197 1197
Size: 5993
BstXI CCAn_nnnn'nTGG
Cuts at: 1237 1237
Size: 5993
BstYI r'GATC_y
Cuts at: 1 252 447 1656 1944 3018 3485 3508 3843
Size: 251 195 1209 288 1074 467 23 335
Cuts at: 3843 4089 5101 5187 5199 5545 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1209 1074 1012 467 449 346 335 288
251 246 195 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2075 2075
Size: 5993
BtgI C'CryG_G
Cuts at: 1112 1112
Size: 5993
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3943 4124 4287 3943
Size: 181 163 5649
Fragments arranged by size:
5649 181 163
BtsI GCAGTG_nn'
Cuts at: 553 1150 3236 553
Size: 597 2086 3310
Fragments arranged by size:
3310 2086 597
DraI TTT'AAA
Cuts at: 2480 2992 2480
Size: 512 5481
DrdI GACnn_nn'nnGTC
Cuts at: 349 3699 4557 349
Size: 3350 858 1785
Fragments arranged by size:
3350 1785 858
EaeI y'GGCC_r
Cuts at: 3578 3752 4143 4170 5515 3578
Size: 174 391 27 1345 4056
Fragments arranged by size:
4056 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3578 3578
Size: 5993
EarI CTCTTCn'nnn_
Cuts at: 777 1179 2431 4016 4226 777
Size: 402 1252 1585 210 2544
Fragments arranged by size:
2544 1585 1252 402 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4201 4511 4657 5803 4201
Size: 310 146 1146 4391
Fragments arranged by size:
4391 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3005 3005
Size: 5993
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 457 1039 1127 1720 1792 2272 2343 2641 2962
Size: 582 88 593 72 480 71 298 321
Cuts at: 2962 3818 4250 4997 5265 457
Size: 856 432 747 268 1185
Fragments arranged by size:
1185 856 747 593 582 480 432 321
298 268 88 72 71
EcoO109I rG'GnC_Cy
Cuts at: 1125 1841 1842 3132 1125
Size: 716 1 1290 3986
Fragments arranged by size:
3986 1290 716 1
EcoRI G'AATT_C
Cuts at: 683 1398 2997 683
Size: 715 1599 3679
Fragments arranged by size:
3679 1599 715
EcoRV GAT'ATC
Cuts at: 1053 2259 1053
Size: 1206 4787
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 865 4012 865
Size: 3147 2846
FspI TGC'GCA
Cuts at: 3774 3774
Size: 5993
HaeII r_GCGC'y
Cuts at: 1803 3427 3675 4697 5676 1803
Size: 1624 248 1022 979 2120
Fragments arranged by size:
2120 1624 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 907 939 1618 1650 1923 1955 2320 2352 2589
Size: 32 679 32 273 32 365 32 237
Cuts at: 2589 2621 2761 2793 3835 3865 3867 3897 907
Size: 32 140 32 1042 30 2 30 3003
Fragments arranged by size:
3003 1042 679 365 273 237 140 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2594 3032 2594
Size: 438 5555
KasI G'GCGC_C
Cuts at: 3423 3671 3423
Size: 248 5745
KpnI G_GTAC'C
Cuts at: 1860 3013 1860
Size: 1153 4840
MfeI C'AATT_G
Cuts at: 830 830
Size: 5993
MluI A'CGCG_T
Cuts at: 5426 5426
Size: 5993
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 833 1809 2193 2980 4664 4848 833
Size: 976 384 787 1684 184 1978
Fragments arranged by size:
1978 1684 976 787 384 184
MscI TGG'CCA
Cuts at: 3754 3754
Size: 5993
MslI CAynn'nnrTG
Cuts at: 2791 3982 4109 2791
Size: 1191 127 4675
Fragments arranged by size:
4675 1191 127
MspA1I CmG'CkG
Cuts at: 1426 2046 3104 3418 3778 4791 5036 1426
Size: 620 1058 314 360 1013 245 2383
Fragments arranged by size:
2383 1058 1013 620 360 314 245
NaeI GCC'GGC
Cuts at: 4174 4174
Size: 5993
NarI GG'CG_CC
Cuts at: 3424 3672 3424
Size: 248 5745
NcoI C'CATG_G
Cuts at: 1112 1112
Size: 5993
NdeI CA'TA_TG
Cuts at: 2781 2781
Size: 5993
NgoMIV G'CCGG_C
Cuts at: 4172 4172
Size: 5993
NsiI A_TGCA'T
Cuts at: 208 972 208
Size: 764 5229
NspI r_CATG'y
Cuts at: 816 1680 3046 3546 4453 816
Size: 864 1366 500 907 2356
Fragments arranged by size:
2356 1366 907 864 500
PciI A'CATG_T
Cuts at: 4449 4449
Size: 5993
PflMI CCAn_nnn'nTGG
Cuts at: 1221 2235 2439 1221
Size: 1014 204 4775
Fragments arranged by size:
4775 1014 204
PfoI T'CCnGG_A
Cuts at: 1820 1820
Size: 5993
PmeI GTTT'AAAC
Cuts at: 2992 2992
Size: 5993
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5132 5164 5132
Size: 32 5961
PsiI TTA'TAA
Cuts at: 647 647
Size: 5993
PspOMI G'GGCC_C
Cuts at: 1841 1841
Size: 5993
PstI C_TGCA'G
Cuts at: 1850 3040 3725 1850
Size: 1190 685 4118
Fragments arranged by size:
4118 1190 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5993
PvuII CAG'CTG
Cuts at: 2046 3418 3778 2046
Size: 1372 360 4261
Fragments arranged by size:
4261 1372 360
RsrII CG'GwC_CG
Cuts at: 4188 4188
Size: 5993
SacI G_AGCT'C
Cuts at: 3007 3007
Size: 5993
SalI G'TCGA_C
Cuts at: 3030 3030
Size: 5993
SapI GCTCTTCn'nnn_
Cuts at: 4016 4226 4016
Size: 210 5783
SbfI CC_TGCA'GG
Cuts at: 1850 3040 1850
Size: 1190 4803
ScaI AGT'ACT
Cuts at: 1045 1549 5510 1045
Size: 504 3961 1528
Fragments arranged by size:
3961 1528 504
SexAI A'CCwGG_T
Cuts at: 1244 1244
Size: 5993
SfcI C'TryA_G
Cuts at: 33 1566 1846 3036 3721 4714 4905 33
Size: 1533 280 1190 685 993 191 1121
Fragments arranged by size:
1533 1190 1121 993 685 280 191
SfoI GGC'GCC
Cuts at: 3425 3673 3425
Size: 248 5745
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5993
SmaI CCC'GGG
Cuts at: 3015 3015
Size: 5993
SmlI C'TyrA_G
Cuts at: 1279 1501 2272 2869 2876 3148 4063 4555 4817
Size: 222 771 597 7 272 915 492 262
Cuts at: 4817 1279
Size: 2455
Fragments arranged by size:
2455 915 771 597 492 272 262 222
7
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5993
SphI G_CATG'C
Cuts at: 3046 3546 3046
Size: 500 5493
SspI AAT'ATT
Cuts at: 2250 2250
Size: 5993
StyI C'CwwG_G
Cuts at: 295 1112 2828 3127 3481 295
Size: 817 1716 299 354 2807
Fragments arranged by size:
2807 1716 817 354 299
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1504 1956 2488 3576 3709 4168 4377 1504
Size: 452 532 1088 133 459 209 3120
Fragments arranged by size:
3120 1088 532 459 452 209 133
TatI w'GTAC_w
Cuts at: 886 1043 1547 5508 886
Size: 157 504 3961 1371
Fragments arranged by size:
3961 1371 504 157
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 237 269 2809 2841 5510 5542 237
Size: 32 2540 32 2669 32 688
Fragments arranged by size:
2669 2540 688 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3790 3790
Size: 5993
XbaI T'CTAG_A
Cuts at: 3024 3024
Size: 5993
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 928 3488 928
Size: 2560 3433
XmaI C'CCGG_G
Cuts at: 3013 3013
Size: 5993
XmnI GAAnn'nnTTC
Cuts at: 290 290
Size: 5993
ZraI GAC'GTC
Cuts at: 1148 2701 1148
Size: 1553 4440
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AhdI AleI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BglI BmgBI BmtI BsaXI BsiWI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI