Restriction map for
pF1KB5680
(Circular) (Six-base) MAPSORT of: pF1KB5680.seq Check: 9726 from: 1 to: 4553
pF1KB5680 4553 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1591 1591
Size: 4553
Acc65I G'GTAC_C
Cuts at: 1569 1569
Size: 4553
AclI AA'CG_TT
Cuts at: 4393 4393
Size: 4553
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 586 766 2378 2810 3163 3825 521
Size: 65 180 1612 432 353 662 1249
Fragments arranged by size:
1612 1249 662 432 353 180 65
AflIII A'CryG_T
Cuts at: 1352 1905 3705 3986 1352
Size: 553 1800 281 1919
Fragments arranged by size:
1919 1800 553 281
AhdI GACnn_n'nnGTC
Cuts at: 468 468
Size: 4553
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 353 385 823 855 353
Size: 32 438 32 4051
Fragments arranged by size:
4051 438 32 32
AlwNI CAG_nnn'CTG
Cuts at: 499 1858 3296 499
Size: 1359 1438 1756
Fragments arranged by size:
1756 1438 1359
ApaI G_GGCC'C
Cuts at: 858 858
Size: 4553
ApaLI G'TGCA_C
Cuts at: 3391 3391
Size: 4553
ApoI r'AATT_y
Cuts at: 1557 3906 4162 1557
Size: 2349 256 1948
Fragments arranged by size:
2349 1948 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4553
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
AvaI C'yCGr_G
Cuts at: 827 1060 1228 1573 827
Size: 233 168 345 3807
Fragments arranged by size:
3807 345 233 168
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1033 1066 1033
Size: 33 4520
BamHI G'GATC_C
Cuts at: 1578 1578
Size: 4553
BanI G'GyrC_C
Cuts at: 205 725 1015 1569 1983 2231 2266 205
Size: 520 290 554 414 248 35 2492
Fragments arranged by size:
2492 554 520 414 290 248 35
BanII G_rGCy'C
Cuts at: 478 858 1187 1294 1567 2597 478
Size: 380 329 107 273 1030 2434
Fragments arranged by size:
2434 1030 380 329 273 107
BbeI G_GCGC'C
Cuts at: 1987 2235 1987
Size: 248 4305
BbsI GAAGACnn'nnnn_
Cuts at: 304 1033 304
Size: 729 3824
BciVI GTATCCnnnnn_n'
Cuts at: 2451 3507 4553 2451
Size: 1056 1046 2451
Fragments arranged by size:
2451 1056 1046
BclI T'GATC_A
Cuts at: 1445 2073 1445
Size: 628 3925
BglII A'GATC_T
Cuts at: 2068 4105 2068
Size: 2037 2516
BlpI GC'TnA_GC
Cuts at: 1369 1665 1369
Size: 296 4257
Bme1580I G_kGCm'C
Cuts at: 586 858 968 2178 2271 3395 586
Size: 272 110 1210 93 1124 1744
Fragments arranged by size:
1744 1210 1124 272 110 93
BmgBI CAC'GTC
Cuts at: 117 1397 117
Size: 1280 3273
BmrI ACTGGGnnnn_n'
Cuts at: 476 1512 1765 1846 2181 4325 476
Size: 1036 253 81 335 2144 704
Fragments arranged by size:
2144 1036 704 335 253 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2920 2920
Size: 4553
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 705 958 1090 705
Size: 253 132 4168
Fragments arranged by size:
4168 253 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 421 634 916 1243 1729 2608 3322 3620
Size: 243 213 282 327 486 879 714 298
Cuts at: 3620 178
Size: 1111
Fragments arranged by size:
1111 879 714 486 327 298 282 243
213
BsaI GGTCTCn'nnnn_
Cuts at: 4257 4257
Size: 4553
BsaAI yAC'GTr
Cuts at: 1353 1906 1353
Size: 553 4000
BsaBI GATnn'nnATC
Cuts at: 6 1450 2067 4104 6
Size: 1444 617 2037 455
Fragments arranged by size:
2037 1444 617 455
BsaHI Gr'CG_yC
Cuts at: 1301 1984 2232 2934 4430 1301
Size: 683 248 702 1496 1424
Fragments arranged by size:
1496 1424 702 683 248
BsaWI w'CCGG_w
Cuts at: 467 1742 2263 2941 3352 3499 3773 467
Size: 1275 521 678 411 147 274 1247
Fragments arranged by size:
1275 1247 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 823 853 823
Size: 30 4523
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 181 403 412 463 466 469 1213 1441 181
Size: 222 9 51 3 3 744 228 3293
Fragments arranged by size:
3293 744 228 222 51 9 3 3
BseYI C'CCAG_C
Cuts at: 573 1110 1978 2736 3401 3809 573
Size: 537 868 758 665 408 1317
Fragments arranged by size:
1317 868 758 665 537 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 883 1112 127
Size: 756 229 3568
Fragments arranged by size:
3568 756 229
BsiEI CG_ry'CG
Cuts at: 104 232 472 771 1089 1143 1547 2095 2141
Size: 128 240 299 318 54 404 548 46
Cuts at: 2141 3371 104
Size: 1230 1286
Fragments arranged by size:
1286 1230 548 404 318 299 240 128
54 46
BsiHKAI G_wGCw'C
Cuts at: 1187 1567 2345 2535 3395 1187
Size: 380 778 190 860 2345
Fragments arranged by size:
2345 860 778 380 190
Bsp1286I G_dGCh'C
Cuts at: 478 586 858 968 1187 1294 1567 2178 2271
Size: 108 272 110 219 107 273 611 93
Cuts at: 2271 2345 2535 2597 3395 478
Size: 74 190 62 798 1636
Fragments arranged by size:
1636 798 611 273 272 219 190 110
108 107 93 74 62
BspHI T'CATG_A
Cuts at: 2985 2985
Size: 4553
BspMI ACCTGCnnnn'nnnn_
Cuts at: 905 1603 1817 2119 2500 905
Size: 698 214 302 381 2958
Fragments arranged by size:
2958 698 381 302 214
BsrBI CCG'CTC
Cuts at: 50 228 306 393 2845 2899 4383 4548 50
Size: 178 78 87 2452 54 1484 165 55
Fragments arranged by size:
2452 1484 178 165 87 78 55 54
BsrDI GCAATG_nn'
Cuts at: 2465 2465
Size: 4553
BsrFI r'CCGG_y
Cuts at: 1333 2551 2732 1333
Size: 1218 181 3154
Fragments arranged by size:
3154 1218 181
BssSI C'ACGA_G
Cuts at: 2824 3532 2824
Size: 708 3845
BstAPI GCAn_nnn'nTGC
Cuts at: 1930 1930
Size: 4553
BstBI TT'CG_AA
Cuts at: 2914 2914
Size: 4553
BstYI r'GATC_y
Cuts at: 1 644 1202 1578 2045 2068 2403 2649 2955
Size: 643 558 376 467 23 335 246 306
Cuts at: 2955 2967 3053 4105 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 643 558 467 449 376 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 386 386
Size: 4553
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1306 2503 2684 2847 1306
Size: 1197 181 163 3012
Fragments arranged by size:
3012 1197 181 163
BtsI GCAGTG_nn'
Cuts at: 289 349 1100 1796 289
Size: 60 751 696 3046
Fragments arranged by size:
3046 751 696 60
DraI TTT'AAA
Cuts at: 1552 1552
Size: 4553
DraIII CAC_nnn'GTG
Cuts at: 484 540 484
Size: 56 4497
DrdI GACnn_nn'nnGTC
Cuts at: 2259 3603 2259
Size: 1344 3209
EaeI y'GGCC_r
Cuts at: 175 229 521 668 1086 1532 1544 2138 2312
Size: 54 292 147 418 446 12 594 174
Cuts at: 2312 2703 2730 4075 175
Size: 391 27 1345 653
Fragments arranged by size:
1345 653 594 446 418 391 292 174
147 54 27 12
EagI C'GGCC_G
Cuts at: 229 1086 1544 2138 229
Size: 857 458 594 2644
Fragments arranged by size:
2644 857 594 458
EarI CTCTTCn'nnn_
Cuts at: 497 2576 2786 497
Size: 2079 210 2264
Fragments arranged by size:
2264 2079 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2761 3503 3649 4363 2761
Size: 742 146 714 2951
Fragments arranged by size:
2951 742 714 146
EcoICRI GAG'CTC
Cuts at: 1185 1565 1185
Size: 380 4173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 521 586 705 766 958 1090 2378 2810 3163
Size: 65 119 61 192 132 1288 432 353
Cuts at: 3163 3825 521
Size: 662 1249
Fragments arranged by size:
1288 1249 662 432 353 192 132 119
65 61
EcoNI CCTnn'n_nnAGG
Cuts at: 607 652 1195 607
Size: 45 543 3965
Fragments arranged by size:
3965 543 45
EcoO109I rG'GnC_Cy
Cuts at: 927 1266 1505 1692 927
Size: 339 239 187 3788
Fragments arranged by size:
3788 339 239 187
EcoRI G'AATT_C
Cuts at: 1557 1557
Size: 4553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2572 2572
Size: 4553
FspI TGC'GCA
Cuts at: 2334 2334
Size: 4553
HaeII r_GCGC'y
Cuts at: 872 1987 2235 3465 4236 872
Size: 1115 248 1230 771 1189
Fragments arranged by size:
1230 1189 1115 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 454 486 865 897 1125 1157 1222 1254 2395
Size: 32 379 32 228 32 65 32 1141
Cuts at: 2395 2425 2427 2457 454
Size: 30 2 30 2550
Fragments arranged by size:
2550 1141 379 228 65 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1592 1592
Size: 4553
HindIII A'AGCT_T
Cuts at: 1174 1174
Size: 4553
KasI G'GCGC_C
Cuts at: 1983 2231 1983
Size: 248 4305
KpnI G_GTAC'C
Cuts at: 1573 1573
Size: 4553
MluI A'CGCG_T
Cuts at: 3986 3986
Size: 4553
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1379 3312 3496 1379
Size: 1933 184 2436
Fragments arranged by size:
2436 1933 184
MscI TGG'CCA
Cuts at: 523 670 2314 523
Size: 147 1644 2762
Fragments arranged by size:
2762 1644 147
MslI CAynn'nnrTG
Cuts at: 1192 2542 2669 1192
Size: 1350 127 3076
Fragments arranged by size:
3076 1350 127
MspA1I CmG'CkG
Cuts at: 291 335 1538 1664 1978 2338 3122 3367 291
Size: 44 1203 126 314 360 784 245 1477
Fragments arranged by size:
1477 1203 784 360 314 245 126 44
NaeI GCC'GGC
Cuts at: 2734 2734
Size: 4553
NarI GG'CG_CC
Cuts at: 1984 2232 1984
Size: 248 4305
NgoMIV G'CCGG_C
Cuts at: 2732 2732
Size: 4553
NotI GC'GGCC_GC
Cuts at: 229 229
Size: 4553
NspI r_CATG'y
Cuts at: 1606 2106 3709 1606
Size: 500 1603 2450
Fragments arranged by size:
2450 1603 500
PciI A'CATG_T
Cuts at: 3705 3705
Size: 4553
PflMI CCAn_nnn'nTGG
Cuts at: 423 423
Size: 4553
PmeI GTTT'AAAC
Cuts at: 1552 1552
Size: 4553
PmlI CAC'GTG
Cuts at: 1353 1353
Size: 4553
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 823 855 2999 3031 823
Size: 32 2144 32 2345
Fragments arranged by size:
2345 2144 32 32
PpuMI rG'GwC_Cy
Cuts at: 927 1266 927
Size: 339 4214
PshAI GACnn'nnGTC
Cuts at: 352 352
Size: 4553
PspOMI G'GGCC_C
Cuts at: 854 854
Size: 4553
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 904 936 904
Size: 32 4521
PstI C_TGCA'G
Cuts at: 344 716 1600 2285 344
Size: 372 884 685 2612
Fragments arranged by size:
2612 884 685 372
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4553
PvuII CAG'CTG
Cuts at: 291 1978 2338 291
Size: 1687 360 2506
Fragments arranged by size:
2506 1687 360
RsrII CG'GwC_CG
Cuts at: 2748 2748
Size: 4553
SacI G_AGCT'C
Cuts at: 1187 1567 1187
Size: 380 4173
SalI G'TCGA_C
Cuts at: 1590 1590
Size: 4553
SapI GCTCTTCn'nnn_
Cuts at: 2576 2786 2576
Size: 210 4343
SbfI CC_TGCA'GG
Cuts at: 1600 1600
Size: 4553
ScaI AGT'ACT
Cuts at: 751 4070 751
Size: 3319 1234
SexAI A'CCwGG_T
Cuts at: 1411 1411
Size: 4553
SfcI C'TryA_G
Cuts at: 33 340 712 1596 2281 3249 3440 33
Size: 307 372 884 685 968 191 1146
Fragments arranged by size:
1146 968 884 685 372 307 191
SfoI GGC'GCC
Cuts at: 1985 2233 1985
Size: 248 4305
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4553
SmaI CCC'GGG
Cuts at: 829 1575 829
Size: 746 3807
SmlI C'TyrA_G
Cuts at: 193 436 649 931 1258 1708 2623 3337 3599
Size: 243 213 282 327 450 915 714 262
Cuts at: 3599 193
Size: 1147
Fragments arranged by size:
1147 915 714 450 327 282 262 243
213
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4553
SphI G_CATG'C
Cuts at: 1606 2106 1606
Size: 500 4053
StyI C'CwwG_G
Cuts at: 671 1304 1687 2041 671
Size: 633 383 354 3183
Fragments arranged by size:
3183 633 383 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 495 2136 2269 2728 2937 495
Size: 1641 133 459 209 2111
Fragments arranged by size:
2111 1641 459 209 133
TatI w'GTAC_w
Cuts at: 158 749 4068 158
Size: 591 3319 643
Fragments arranged by size:
3319 643 591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 170 202 1209 1241 4070 4102 170
Size: 32 1007 32 2829 32 621
Fragments arranged by size:
2829 1007 621 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2350 2350
Size: 4553
XbaI T'CTAG_A
Cuts at: 1584 1584
Size: 4553
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 420 678 2048 114
Size: 306 258 1370 2619
Fragments arranged by size:
2619 1370 306 258
XmaI C'CCGG_G
Cuts at: 827 1573 827
Size: 746 3807
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AloI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NotI NspI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AscI
AvrII BbvCI BcgI BfrBI BglI BmtI Bpu10I BsiWI
BsmI BsmBI BspEI BsrGI BssHII BstEII BstXI BstZ17I
BtgI ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NruI NsiI PacI PasI PfoI
PsiI PspXI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI