Restriction map for
pF1KB5682
(Circular) (Six-base) MAPSORT of: pF1KB5682.seq Check: 9864 from: 1 to: 4769
pF1KB5682 4769 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 789 1807 789
Size: 1018 3751
Acc65I G'GTAC_C
Cuts at: 1785 1785
Size: 4769
AclI AA'CG_TT
Cuts at: 4609 4609
Size: 4769
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 337 650 955 2594 3026 3379 4041 268
Size: 69 313 305 1639 432 353 662 996
Fragments arranged by size:
1639 996 662 432 353 313 305 69
AflIII A'CryG_T
Cuts at: 438 2121 3921 4202 438
Size: 1683 1800 281 1005
Fragments arranged by size:
1800 1683 1005 281
AhdI GACnn_n'nnGTC
Cuts at: 117 117
Size: 4769
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 575 1282 575
Size: 707 4062
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1367 1399 1367
Size: 32 4737
AlwNI CAG_nnn'CTG
Cuts at: 1483 1567 2074 3512 1483
Size: 84 507 1438 2740
Fragments arranged by size:
2740 1438 507 84
ApaI G_GGCC'C
Cuts at: 1704 1704
Size: 4769
ApaLI G'TGCA_C
Cuts at: 1367 3607 1367
Size: 2240 2529
ApoI r'AATT_y
Cuts at: 1685 1773 4122 4378 1685
Size: 88 2349 256 2076
Fragments arranged by size:
2349 2076 256 88
AseI AT'TA_AT
Cuts at: 20 20
Size: 4769
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
AvaI C'yCGr_G
Cuts at: 1789 1789
Size: 4769
BamHI G'GATC_C
Cuts at: 1794 1794
Size: 4769
BanI G'GyrC_C
Cuts at: 1307 1785 2199 2447 2482 1307
Size: 478 414 248 35 3594
Fragments arranged by size:
3594 478 414 248 35
BanII G_rGCy'C
Cuts at: 306 1704 1783 2813 306
Size: 1398 79 1030 2262
Fragments arranged by size:
2262 1398 1030 79
BbeI G_GCGC'C
Cuts at: 2203 2451 2203
Size: 248 4521
BbvCI CC'TCA_GC
Cuts at: 839 839
Size: 4769
BciVI GTATCCnnnnn_n'
Cuts at: 1156 2667 3723 4769 1156
Size: 1511 1056 1046 1156
Fragments arranged by size:
1511 1156 1056 1046
BclI T'GATC_A
Cuts at: 2289 2289
Size: 4769
BfrBI ATG'CAT
Cuts at: 1604 1604
Size: 4769
BglII A'GATC_T
Cuts at: 1391 2284 4321 1391
Size: 893 2037 1839
Fragments arranged by size:
2037 1839 893
BlpI GC'TnA_GC
Cuts at: 1881 1881
Size: 4769
Bme1580I G_kGCm'C
Cuts at: 1371 1704 2394 2487 3611 1371
Size: 333 690 93 1124 2529
Fragments arranged by size:
2529 1124 690 333 93
BmrI ACTGGGnnnn_n'
Cuts at: 802 1981 2062 2397 4541 802
Size: 1179 81 335 2144 1030
Fragments arranged by size:
2144 1179 1030 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 578 3136 578
Size: 2558 2211
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 423 1199 1394 1529 1690 423
Size: 776 195 135 161 3502
Fragments arranged by size:
3502 776 195 161 135
Bpu10I CC'TnA_GC
Cuts at: 839 839
Size: 4769
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1945 2824 3538 3836 1945
Size: 879 714 298 2878
Fragments arranged by size:
2878 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4473 4473
Size: 4769
BsaAI yAC'GTr
Cuts at: 1167 2122 1167
Size: 955 3814
BsaBI GATnn'nnATC
Cuts at: 6 2283 4320 6
Size: 2277 2037 455
Fragments arranged by size:
2277 2037 455
BsaHI Gr'CG_yC
Cuts at: 2200 2448 3150 4646 2200
Size: 248 702 1496 2323
Fragments arranged by size:
2323 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1638 1958 2479 3157 3568 3715 3989 1638
Size: 320 521 678 411 147 274 2418
Fragments arranged by size:
2418 678 521 411 320 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1367 1397 1367
Size: 30 4739
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1196 1196
Size: 4769
BseYI C'CCAG_C
Cuts at: 699 1360 1631 2194 2952 3617 4025 699
Size: 661 271 563 758 665 408 1443
Fragments arranged by size:
1443 758 665 661 563 408 271
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1288 1451 911
Size: 377 163 4229
Fragments arranged by size:
4229 377 163
BsiEI CG_ry'CG
Cuts at: 104 2311 2357 3587 104
Size: 2207 46 1230 1286
Fragments arranged by size:
2207 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 306 1371 1783 2561 2751 3611 306
Size: 1065 412 778 190 860 1464
Fragments arranged by size:
1464 1065 860 778 412 190
BsmI GAATG_Cn'
Cuts at: 1033 1033
Size: 4769
BsmBI CGTCTCn'nnnn_
Cuts at: 403 403
Size: 4769
Bsp1286I G_dGCh'C
Cuts at: 306 1371 1704 1783 2394 2487 2561 2751 2813
Size: 1065 333 79 611 93 74 190 62
Cuts at: 2813 3611 306
Size: 798 1464
Fragments arranged by size:
1464 1065 798 611 333 190 93 79
74 62
BspHI T'CATG_A
Cuts at: 3201 3201
Size: 4769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1819 2033 2335 2716 1819
Size: 214 302 381 3872
Fragments arranged by size:
3872 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3061 3115 4599 4764 50
Size: 3011 54 1484 165 55
Fragments arranged by size:
3011 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1082 2681 1082
Size: 1599 3170
BsrFI r'CCGG_y
Cuts at: 2767 2948 2767
Size: 181 4588
BsrGI T'GTAC_A
Cuts at: 1332 1332
Size: 4769
BssSI C'ACGA_G
Cuts at: 3040 3748 3040
Size: 708 4061
BstAPI GCAn_nnn'nTGC
Cuts at: 1474 1483 2146 1474
Size: 9 663 4097
Fragments arranged by size:
4097 663 9
BstBI TT'CG_AA
Cuts at: 3130 3130
Size: 4769
BstXI CCAn_nnnn'nTGG
Cuts at: 792 792
Size: 4769
BstYI r'GATC_y
Cuts at: 1 1391 1794 2261 2284 2619 2865 3171 3183
Size: 1390 403 467 23 335 246 306 12
Cuts at: 3183 3269 4321 1
Size: 86 1052 449
Fragments arranged by size:
1390 1052 467 449 403 335 306 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 790 790
Size: 4769
BtgI C'CryG_G
Cuts at: 107 1499 107
Size: 1392 3377
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2719 2900 3063 2719
Size: 181 163 4425
Fragments arranged by size:
4425 181 163
BtsI GCAGTG_nn'
Cuts at: 547 737 2012 547
Size: 190 1275 3304
Fragments arranged by size:
3304 1275 190
DraI TTT'AAA
Cuts at: 1768 1768
Size: 4769
DrdI GACnn_nn'nnGTC
Cuts at: 1324 2475 3819 1324
Size: 1151 1344 2274
Fragments arranged by size:
2274 1344 1151
EaeI y'GGCC_r
Cuts at: 963 2354 2528 2919 2946 4291 963
Size: 1391 174 391 27 1345 1441
Fragments arranged by size:
1441 1391 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2354 2354
Size: 4769
EarI CTCTTCn'nnn_
Cuts at: 2792 3002 2792
Size: 210 4559
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2977 3719 3865 4579 2977
Size: 742 146 714 3167
Fragments arranged by size:
3167 742 714 146
EcoICRI GAG'CTC
Cuts at: 304 1781 304
Size: 1477 3292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 268 337 423 650 955 1199 1394 1529 1690
Size: 69 86 227 305 244 195 135 161
Cuts at: 1690 2594 3026 3379 4041 268
Size: 904 432 353 662 996
Fragments arranged by size:
996 904 662 432 353 305 244 227
195 161 135 86 69
EcoNI CCTnn'n_nnAGG
Cuts at: 842 1178 1520 842
Size: 336 342 4091
Fragments arranged by size:
4091 342 336
EcoO109I rG'GnC_Cy
Cuts at: 835 1701 1908 835
Size: 866 207 3696
Fragments arranged by size:
3696 866 207
EcoRI G'AATT_C
Cuts at: 1773 1773
Size: 4769
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2788 2788
Size: 4769
FspI TGC'GCA
Cuts at: 2550 2550
Size: 4769
HaeII r_GCGC'y
Cuts at: 2203 2451 3681 4452 2203
Size: 248 1230 771 2520
Fragments arranged by size:
2520 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 1643 1675 2611 2641 2643 2673 103
Size: 32 1508 32 936 30 2 30 2199
Fragments arranged by size:
2199 1508 936 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1204 1808 1204
Size: 604 4165
KasI G'GCGC_C
Cuts at: 2199 2447 2199
Size: 248 4521
KpnI G_GTAC'C
Cuts at: 1789 1789
Size: 4769
MfeI C'AATT_G
Cuts at: 224 224
Size: 4769
MluI A'CGCG_T
Cuts at: 4202 4202
Size: 4769
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 346 1253 3528 3712 346
Size: 907 2275 184 1403
Fragments arranged by size:
2275 1403 907 184
MscI TGG'CCA
Cuts at: 2530 2530
Size: 4769
MslI CAynn'nnrTG
Cuts at: 425 760 2758 2885 425
Size: 335 1998 127 2309
Fragments arranged by size:
2309 1998 335 127
MspA1I CmG'CkG
Cuts at: 1483 1564 1880 2194 2554 3338 3583 1483
Size: 81 316 314 360 784 245 2669
Fragments arranged by size:
2669 784 360 316 314 245 81
NaeI GCC'GGC
Cuts at: 2950 2950
Size: 4769
NarI GG'CG_CC
Cuts at: 2200 2448 2200
Size: 248 4521
NcoI C'CATG_G
Cuts at: 107 107
Size: 4769
NgoMIV G'CCGG_C
Cuts at: 2948 2948
Size: 4769
NsiI A_TGCA'T
Cuts at: 1606 1606
Size: 4769
NspI r_CATG'y
Cuts at: 442 1822 2322 3925 442
Size: 1380 500 1603 1286
Fragments arranged by size:
1603 1380 1286 500
PciI A'CATG_T
Cuts at: 438 3921 438
Size: 3483 1286
PfoI T'CCnGG_A
Cuts at: 999 999
Size: 4769
PmeI GTTT'AAAC
Cuts at: 1768 1768
Size: 4769
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1367 1399 3215 3247 1367
Size: 32 1816 32 2889
Fragments arranged by size:
2889 1816 32 32
PshAI GACnn'nnGTC
Cuts at: 1592 1592
Size: 4769
PspOMI G'GGCC_C
Cuts at: 1700 1700
Size: 4769
PstI C_TGCA'G
Cuts at: 554 1479 1816 2501 554
Size: 925 337 685 2822
Fragments arranged by size:
2822 925 685 337
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4769
PvuII CAG'CTG
Cuts at: 1483 1564 2194 2554 1483
Size: 81 630 360 3698
Fragments arranged by size:
3698 630 360 81
RsrII CG'GwC_CG
Cuts at: 2964 2964
Size: 4769
SacI G_AGCT'C
Cuts at: 306 1783 306
Size: 1477 3292
SalI G'TCGA_C
Cuts at: 1806 1806
Size: 4769
SapI GCTCTTCn'nnn_
Cuts at: 2792 3002 2792
Size: 210 4559
SbfI CC_TGCA'GG
Cuts at: 1816 1816
Size: 4769
ScaI AGT'ACT
Cuts at: 1724 4286 1724
Size: 2562 2207
SfcI C'TryA_G
Cuts at: 33 550 1475 1812 2497 3465 3656 33
Size: 517 925 337 685 968 191 1146
Fragments arranged by size:
1146 968 925 685 517 337 191
SfoI GGC'GCC
Cuts at: 2201 2449 2201
Size: 248 4521
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4769
SmaI CCC'GGG
Cuts at: 1791 1791
Size: 4769
SmlI C'TyrA_G
Cuts at: 1924 2839 3553 3815 1924
Size: 915 714 262 2878
Fragments arranged by size:
2878 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4769
SphI G_CATG'C
Cuts at: 1822 2322 1822
Size: 500 4269
SspI AAT'ATT
Cuts at: 448 448
Size: 4769
StyI C'CwwG_G
Cuts at: 107 1903 2257 107
Size: 1796 354 2619
Fragments arranged by size:
2619 1796 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2352 2485 2944 3153 2352
Size: 133 459 209 3968
Fragments arranged by size:
3968 459 209 133
TatI w'GTAC_w
Cuts at: 1332 1722 4284 1332
Size: 390 2562 1817
Fragments arranged by size:
2562 1817 390
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4286 4318 4286
Size: 32 4737
Tth111I GACn'n_nGTC
Cuts at: 2566 2566
Size: 4769
XbaI T'CTAG_A
Cuts at: 1800 1800
Size: 4769
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 190 759 2264 190
Size: 569 1505 2695
Fragments arranged by size:
2695 1505 569
XmaI C'CCGG_G
Cuts at: 1789 1789
Size: 4769
XmnI GAAnn'nnTTC
Cuts at: 1724 1724
Size: 4769
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PciI PfoI PmeI PpiI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AscI AvrII
BaeI BbsI BcgI BglI BmgBI BmtI BsiWI BspEI
BssHII BstEII Bsu36I ClaI CspCI DraIII EcoRV FseI
FspAI HindIII HpaI NdeI NheI NotI NruI PacI
PasI PflMI PmlI PpuMI PsiI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI