Restriction map for
pF1KB5687
(Circular) (Six-base) MAPSORT of: pF1KB5687.seq Check: 2839 from: 1 to: 3845
pF1KB5687 3845 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AccI GT'mk_AC
Cuts at: 423 883 423
Size: 460 3385
Acc65I G'GTAC_C
Cuts at: 861 861
Size: 3845
AclI AA'CG_TT
Cuts at: 719 3685 719
Size: 2966 879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1670 2102 2849 3117 1670
Size: 432 747 268 2398
Fragments arranged by size:
2398 747 432 268
AflIII A'CryG_T
Cuts at: 1197 2301 3278 1197
Size: 1104 977 1764
Fragments arranged by size:
1764 1104 977
AgeI A'CCGG_T
Cuts at: 2233 2233
Size: 3845
AlwNI CAG_nnn'CTG
Cuts at: 1150 2717 1150
Size: 1567 2278
ApaLI G'TGCA_C
Cuts at: 2615 2615
Size: 3845
ApoI r'AATT_y
Cuts at: 475 849 3198 3454 475
Size: 374 2349 256 866
Fragments arranged by size:
2349 866 374 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3845
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
AvaI C'yCGr_G
Cuts at: 253 865 253
Size: 612 3233
BamHI G'GATC_C
Cuts at: 156 870 156
Size: 714 3131
BanI G'GyrC_C
Cuts at: 861 1275 1523 1558 861
Size: 414 248 35 3148
Fragments arranged by size:
3148 414 248 35
BanII G_rGCy'C
Cuts at: 554 859 1889 554
Size: 305 1030 2510
Fragments arranged by size:
2510 1030 305
BbeI G_GCGC'C
Cuts at: 1279 1527 1279
Size: 248 3597
BbsI GAAGACnn'nnnn_
Cuts at: 225 225
Size: 3845
BciVI GTATCCnnnnn_n'
Cuts at: 1743 2504 3845 1743
Size: 761 1341 1743
Fragments arranged by size:
1743 1341 761
BclI T'GATC_A
Cuts at: 1365 1365
Size: 3845
BglI GCCn_nnn'nGGC
Cuts at: 691 691
Size: 3845
BglII A'GATC_T
Cuts at: 1360 3397 1360
Size: 2037 1808
BlpI GC'TnA_GC
Cuts at: 639 957 639
Size: 318 3527
Bme1580I G_kGCm'C
Cuts at: 1470 1563 2619 1470
Size: 93 1056 2696
Fragments arranged by size:
2696 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1057 1138 1473 3617 1057
Size: 81 335 2144 1285
Fragments arranged by size:
2144 1285 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2212 2212
Size: 3845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1021 1900 2392 2690 1021
Size: 879 492 298 2176
Fragments arranged by size:
2176 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 424 3549 424
Size: 3125 720
BsaAI yAC'GTr
Cuts at: 1198 1198
Size: 3845
BsaBI GATnn'nnATC
Cuts at: 6 161 1359 3396 6
Size: 155 1198 2037 455
Fragments arranged by size:
2037 1198 455 155
BsaHI Gr'CG_yC
Cuts at: 571 1276 1524 2226 3722 571
Size: 705 248 702 1496 694
Fragments arranged by size:
1496 705 702 694 248
BsaWI w'CCGG_w
Cuts at: 1034 1555 2233 2507 2654 3065 1034
Size: 521 678 274 147 411 1814
Fragments arranged by size:
1814 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1270 2028 2605 3101 1270
Size: 758 577 496 2014
Fragments arranged by size:
2014 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 813
Size: 3845
BsiEI CG_ry'CG
Cuts at: 104 1387 1433 2641 104
Size: 1283 46 1208 1308
Fragments arranged by size:
1308 1283 1208 46
BsiHKAI G_wGCw'C
Cuts at: 554 859 1637 1827 2619 554
Size: 305 778 190 792 1780
Fragments arranged by size:
1780 792 778 305 190
BsmI GAATG_Cn'
Cuts at: 640 640
Size: 3845
Bsp1286I G_dGCh'C
Cuts at: 554 859 1470 1563 1637 1827 1889 2619 554
Size: 305 611 93 74 190 62 730 1780
Fragments arranged by size:
1780 730 611 305 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3065 3065
Size: 3845
BspHI T'CATG_A
Cuts at: 3021 3021
Size: 3845
BspMI ACCTGCnnnn'nnnn_
Cuts at: 895 1109 1411 1792 895
Size: 214 302 381 2948
Fragments arranged by size:
2948 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2137 2191 3675 3840 50
Size: 2087 54 1484 165 55
Fragments arranged by size:
2087 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1757 1757
Size: 3845
BsrFI r'CCGG_y
Cuts at: 1843 2024 2233 1843
Size: 181 209 3455
Fragments arranged by size:
3455 209 181
BssSI C'ACGA_G
Cuts at: 2116 2474 2116
Size: 358 3487
BstAPI GCAn_nnn'nTGC
Cuts at: 1222 1222
Size: 3845
BstBI TT'CG_AA
Cuts at: 2206 2206
Size: 3845
BstYI r'GATC_y
Cuts at: 1 156 870 1337 1360 1695 1941 2953 3039
Size: 155 714 467 23 335 246 1012 86
Cuts at: 3039 3051 3397 1
Size: 12 346 449
Fragments arranged by size:
1012 714 467 449 346 335 246 155
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1795 1976 2139 1795
Size: 181 163 3501
Fragments arranged by size:
3501 181 163
BtsI GCAGTG_nn'
Cuts at: 1088 1088
Size: 3845
DraI TTT'AAA
Cuts at: 844 844
Size: 3845
DrdI GACnn_nn'nnGTC
Cuts at: 576 1551 2409 576
Size: 975 858 2012
Fragments arranged by size:
2012 975 858
EaeI y'GGCC_r
Cuts at: 290 602 1430 1604 1995 2022 3367 290
Size: 312 828 174 391 27 1345 768
Fragments arranged by size:
1345 828 768 391 312 174 27
EagI C'GGCC_G
Cuts at: 1430 1430
Size: 3845
EarI CTCTTCn'nnn_
Cuts at: 114 817 1868 2078 114
Size: 703 1051 210 1881
Fragments arranged by size:
1881 1051 703 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 358 2053 2363 2509 3655 358
Size: 1695 310 146 1146 548
Fragments arranged by size:
1695 1146 548 310 146
EcoICRI GAG'CTC
Cuts at: 552 857 552
Size: 305 3540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1670 2102 2849 3117 1670
Size: 432 747 268 2398
Fragments arranged by size:
2398 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 984 984
Size: 3845
EcoRI G'AATT_C
Cuts at: 849 849
Size: 3845
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 264 1864 264
Size: 1600 2245
FspI TGC'GCA
Cuts at: 1626 1626
Size: 3845
HaeII r_GCGC'y
Cuts at: 1279 1527 2549 3528 1279
Size: 248 1022 979 1596
Fragments arranged by size:
1596 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 767 799 1687 1717 1719 1749 767
Size: 32 888 30 2 30 2863
Fragments arranged by size:
2863 888 32 30 30 2
HincII GTy'rAC
Cuts at: 884 884
Size: 3845
KasI G'GCGC_C
Cuts at: 1275 1523 1275
Size: 248 3597
KpnI G_GTAC'C
Cuts at: 865 865
Size: 3845
MluI A'CGCG_T
Cuts at: 3278 3278
Size: 3845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 632 2516 2700 632
Size: 1884 184 1777
Fragments arranged by size:
1884 1777 184
MscI TGG'CCA
Cuts at: 604 1606 604
Size: 1002 2843
MslI CAynn'nnrTG
Cuts at: 835 1834 1961 835
Size: 999 127 2719
Fragments arranged by size:
2719 999 127
MspA1I CmG'CkG
Cuts at: 956 1270 1630 2643 2888 956
Size: 314 360 1013 245 1913
Fragments arranged by size:
1913 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2026 2026
Size: 3845
NarI GG'CG_CC
Cuts at: 1276 1524 1276
Size: 248 3597
NgoMIV G'CCGG_C
Cuts at: 2024 2024
Size: 3845
NspI r_CATG'y
Cuts at: 698 768 898 1398 2305 698
Size: 70 130 500 907 2238
Fragments arranged by size:
2238 907 500 130 70
PciI A'CATG_T
Cuts at: 2301 2301
Size: 3845
PfoI T'CCnGG_A
Cuts at: 470 470
Size: 3845
PmeI GTTT'AAAC
Cuts at: 844 844
Size: 3845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2984 3016 2984
Size: 32 3813
PstI C_TGCA'G
Cuts at: 892 1577 892
Size: 685 3160
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3845
PvuII CAG'CTG
Cuts at: 1270 1630 1270
Size: 360 3485
RsrII CG'GwC_CG
Cuts at: 2040 2040
Size: 3845
SacI G_AGCT'C
Cuts at: 554 859 554
Size: 305 3540
SalI G'TCGA_C
Cuts at: 882 882
Size: 3845
SapI GCTCTTCn'nnn_
Cuts at: 114 817 1868 2078 114
Size: 703 1051 210 1881
Fragments arranged by size:
1881 1051 703 210
SbfI CC_TGCA'GG
Cuts at: 892 892
Size: 3845
ScaI AGT'ACT
Cuts at: 3362 3362
Size: 3845
SfcI C'TryA_G
Cuts at: 33 269 888 1573 2566 2757 33
Size: 236 619 685 993 191 1121
Fragments arranged by size:
1121 993 685 619 236 191
SfoI GGC'GCC
Cuts at: 1277 1525 1277
Size: 248 3597
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3845
SmaI CCC'GGG
Cuts at: 867 867
Size: 3845
SmlI C'TyrA_G
Cuts at: 1000 1915 2407 2669 1000
Size: 915 492 262 2176
Fragments arranged by size:
2176 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3845
SphI G_CATG'C
Cuts at: 898 1398 898
Size: 500 3345
StyI C'CwwG_G
Cuts at: 239 689 979 1333 239
Size: 450 290 354 2751
Fragments arranged by size:
2751 450 354 290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1428 1561 2020 2229 1428
Size: 133 459 209 3044
Fragments arranged by size:
3044 459 209 133
TatI w'GTAC_w
Cuts at: 538 3360 538
Size: 2822 1023
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3362 3394 3362
Size: 32 3813
Tth111I GACn'n_nGTC
Cuts at: 364 442 1642 364
Size: 78 1200 2567
Fragments arranged by size:
2567 1200 78
XbaI T'CTAG_A
Cuts at: 876 876
Size: 3845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 498 1340 498
Size: 842 3003
XmaI C'CCGG_G
Cuts at: 865 865
Size: 3845
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
BmtI BpmI Bpu10I BsaXI BseRI BsiWI BsmBI BsrGI
BssHII BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI XmnI ZraI