Restriction map for
pF1KB5691
(Circular) (Six-base) MAPSORT of: pF1KB5691.seq Check: 2208 from: 1 to: 5693
pF1KB5691 5693 bp
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AccI GT'mk_AC
Cuts at: 678 2731 678
Size: 2053 3640
Acc65I G'GTAC_C
Cuts at: 2709 2709
Size: 5693
AclI AA'CG_TT
Cuts at: 479 5533 479
Size: 5054 639
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 911 1318 1748 1922 2006 2044 2151 3518 3950
Size: 407 430 174 84 38 107 1367 432
Cuts at: 3950 4697 4965 911
Size: 747 268 1639
Fragments arranged by size:
1639 1367 747 432 430 407 268 174
107 84 38
AflIII A'CryG_T
Cuts at: 520 3045 4149 5126 520
Size: 2525 1104 977 1087
Fragments arranged by size:
2525 1104 1087 977
AgeI A'CCGG_T
Cuts at: 2627 4081 2627
Size: 1454 4239
AlwNI CAG_nnn'CTG
Cuts at: 1063 2125 2998 4565 1063
Size: 1062 873 1567 2191
Fragments arranged by size:
2191 1567 1062 873
ApaLI G'TGCA_C
Cuts at: 4463 4463
Size: 5693
ApoI r'AATT_y
Cuts at: 214 2697 5046 5302 214
Size: 2483 2349 256 605
Fragments arranged by size:
2483 2349 605 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5693
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5693
AvaI C'yCGr_G
Cuts at: 113 163 2713 113
Size: 50 2550 3093
Fragments arranged by size:
3093 2550 50
BamHI G'GATC_C
Cuts at: 2718 2718
Size: 5693
BanI G'GyrC_C
Cuts at: 383 2709 3123 3371 3406 383
Size: 2326 414 248 35 2670
Fragments arranged by size:
2670 2326 414 248 35
BanII G_rGCy'C
Cuts at: 1510 1687 2707 3737 1510
Size: 177 1020 1030 3466
Fragments arranged by size:
3466 1030 1020 177
BbeI G_GCGC'C
Cuts at: 3127 3375 3127
Size: 248 5445
BciVI GTATCCnnnnn_n'
Cuts at: 453 2199 3591 4352 5693 453
Size: 1746 1392 761 1341 453
Fragments arranged by size:
1746 1392 1341 761 453
BclI T'GATC_A
Cuts at: 3213 3213
Size: 5693
BglII A'GATC_T
Cuts at: 1210 2465 3208 5245 1210
Size: 1255 743 2037 1658
Fragments arranged by size:
2037 1658 1255 743
BlpI GC'TnA_GC
Cuts at: 1504 2360 2805 1504
Size: 856 445 4392
Fragments arranged by size:
4392 856 445
Bme1580I G_kGCm'C
Cuts at: 386 784 3318 3411 4467 386
Size: 398 2534 93 1056 1612
Fragments arranged by size:
2534 1612 1056 398 93
BmgBI CAC'GTC
Cuts at: 147 682 692 147
Size: 535 10 5148
Fragments arranged by size:
5148 535 10
BmrI ACTGGGnnnn_n'
Cuts at: 1777 2024 2083 2905 2986 3321 5465 1777
Size: 247 59 822 81 335 2144 2005
Fragments arranged by size:
2144 2005 822 335 247 81 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4060 4060
Size: 5693
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 314 473 1301 1409 2247 190
Size: 124 159 828 108 838 3636
Fragments arranged by size:
3636 838 828 159 124 108
Bpu10I CC'TnA_GC
Cuts at: 1364 1988 1364
Size: 624 5069
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2869 3748 4240 4538 2869
Size: 879 492 298 4024
Fragments arranged by size:
4024 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5397 5397
Size: 5693
BsaAI yAC'GTr
Cuts at: 521 3046 521
Size: 2525 3168
BsaBI GATnn'nnATC
Cuts at: 6 3207 5244 6
Size: 3201 2037 455
Fragments arranged by size:
3201 2037 455
BsaHI Gr'CG_yC
Cuts at: 3124 3372 4074 5570 3124
Size: 248 702 1496 3247
Fragments arranged by size:
3247 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2627 2882 3403 4081 4355 4502 4913 2627
Size: 255 521 678 274 147 411 3407
Fragments arranged by size:
3407 678 521 411 274 255 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 163 193 446 476 1368 1398 1707 1737 163
Size: 30 253 30 892 30 309 30 4119
Fragments arranged by size:
4119 892 309 253 30 30 30 30
BseYI C'CCAG_C
Cuts at: 777 3118 3876 4453 4949 777
Size: 2341 758 577 496 1521
Fragments arranged by size:
2341 1521 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 621
Size: 5693
BsiEI CG_ry'CG
Cuts at: 104 3235 3281 4489 104
Size: 3131 46 1208 1308
Fragments arranged by size:
3131 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1687 2707 3485 3675 4467 1687
Size: 1020 778 190 792 2913
Fragments arranged by size:
2913 1020 792 778 190
BsmI GAATG_Cn'
Cuts at: 779 779
Size: 5693
Bsp1286I G_dGCh'C
Cuts at: 386 784 1510 1687 2707 3318 3411 3485 3675
Size: 398 726 177 1020 611 93 74 190
Cuts at: 3675 3737 4467 386
Size: 62 730 1612
Fragments arranged by size:
1612 1020 730 726 611 398 190 177
93 74 62
BspEI T'CCGG_A
Cuts at: 4913 4913
Size: 5693
BspHI T'CATG_A
Cuts at: 2028 4869 2028
Size: 2841 2852
BspMI ACCTGCnnnn'nnnn_
Cuts at: 796 2743 2957 3259 3640 796
Size: 1947 214 302 381 2849
Fragments arranged by size:
2849 1947 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3985 4039 5523 5688 50
Size: 3935 54 1484 165 55
Fragments arranged by size:
3935 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1307 1641 3605 1307
Size: 334 1964 3395
Fragments arranged by size:
3395 1964 334
BsrFI r'CCGG_y
Cuts at: 2627 3691 3872 4081 2627
Size: 1064 181 209 4239
Fragments arranged by size:
4239 1064 209 181
BsrGI T'GTAC_A
Cuts at: 421 421
Size: 5693
BssSI C'ACGA_G
Cuts at: 968 3964 4322 968
Size: 2996 358 2339
Fragments arranged by size:
2996 2339 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2370 3070 2370
Size: 700 4993
BstBI TT'CG_AA
Cuts at: 4054 4054
Size: 5693
BstEII G'GTnAC_C
Cuts at: 2471 2471
Size: 5693
BstXI CCAn_nnnn'nTGG
Cuts at: 1836 1836
Size: 5693
BstYI r'GATC_y
Cuts at: 1 1210 2465 2718 3185 3208 3543 3789 4801
Size: 1209 1255 253 467 23 335 246 1012
Cuts at: 4801 4887 4899 5245 1
Size: 86 12 346 449
Fragments arranged by size:
1255 1209 1012 467 449 346 335 253
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 3643 3824 3987 2239
Size: 1404 181 163 3945
Fragments arranged by size:
3945 1404 181 163
BtsI GCAGTG_nn'
Cuts at: 2354 2936 2354
Size: 582 5111
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 119 154 678 713 119
Size: 35 524 35 5099
Fragments arranged by size:
5099 524 35 35
DraI TTT'AAA
Cuts at: 2692 2692
Size: 5693
DrdI GACnn_nn'nnGTC
Cuts at: 3399 4257 3399
Size: 858 4835
EaeI y'GGCC_r
Cuts at: 2328 3278 3452 3843 3870 5215 2328
Size: 950 174 391 27 1345 2806
Fragments arranged by size:
2806 1345 950 391 174 27
EagI C'GGCC_G
Cuts at: 3278 3278
Size: 5693
EarI CTCTTCn'nnn_
Cuts at: 204 930 989 996 1001 1259 2063 2477 3716
Size: 726 59 7 5 258 804 414 1239
Cuts at: 3716 3926 204
Size: 210 1971
Fragments arranged by size:
1971 1239 804 726 414 258 210 59
7 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3901 4211 4357 5503 3901
Size: 310 146 1146 4091
Fragments arranged by size:
4091 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1685 2705 1685
Size: 1020 4673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 190 314 473 911 1301 1318 1409 1748 1922
Size: 124 159 438 390 17 91 339 174
Cuts at: 1922 2006 2044 2151 2247 3518 3950 4697 4965
Size: 84 38 107 96 1271 432 747 268
Cuts at: 4965 190
Size: 918
Fragments arranged by size:
1271 918 747 438 432 390 339 268
174 159 124 107 96 91 84 38
17
EcoO109I rG'GnC_Cy
Cuts at: 158 174 2122 2832 158
Size: 16 1948 710 3019
Fragments arranged by size:
3019 1948 710 16
EcoRI G'AATT_C
Cuts at: 2697 2697
Size: 5693
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 262 3712 262
Size: 3450 2243
FspI TGC'GCA
Cuts at: 3474 3474
Size: 5693
HaeII r_GCGC'y
Cuts at: 3127 3375 4397 5376 3127
Size: 248 1022 979 3444
Fragments arranged by size:
3444 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 163 195 203 235 1138 1170 1368 1400 1740
Size: 32 8 32 903 32 198 32 340
Cuts at: 1740 1772 1831 1863 2122 2154 3535 3565 3567
Size: 32 59 32 259 32 1381 30 2
Cuts at: 3567 3597 163
Size: 30 2259
Fragments arranged by size:
2259 1381 903 340 259 198 59 32
32 32 32 32 32 32 30 30
8 2
HincII GTy'rAC
Cuts at: 679 2732 679
Size: 2053 3640
KasI G'GCGC_C
Cuts at: 3123 3371 3123
Size: 248 5445
KpnI G_GTAC'C
Cuts at: 2713 2713
Size: 5693
MluI A'CGCG_T
Cuts at: 5126 5126
Size: 5693
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 262 1868 2612 4364 4548 262
Size: 1606 744 1752 184 1407
Fragments arranged by size:
1752 1606 1407 744 184
MscI TGG'CCA
Cuts at: 2330 3454 2330
Size: 1124 4569
MslI CAynn'nnrTG
Cuts at: 882 3682 3809 882
Size: 2800 127 2766
Fragments arranged by size:
2800 2766 127
MspA1I CmG'CkG
Cuts at: 1321 2804 3118 3478 4491 4736 1321
Size: 1483 314 360 1013 245 2278
Fragments arranged by size:
2278 1483 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3874 3874
Size: 5693
NarI GG'CG_CC
Cuts at: 3124 3372 3124
Size: 248 5445
NgoMIV G'CCGG_C
Cuts at: 3872 3872
Size: 5693
NspI r_CATG'y
Cuts at: 2746 3246 4153 2746
Size: 500 907 4286
Fragments arranged by size:
4286 907 500
PacI TTA_AT'TAA
Cuts at: 671 671
Size: 5693
PciI A'CATG_T
Cuts at: 4149 4149
Size: 5693
PflMI CCAn_nnn'nTGG
Cuts at: 2291 2291
Size: 5693
PfoI T'CCnGG_A
Cuts at: 2263 2263
Size: 5693
PmeI GTTT'AAAC
Cuts at: 2692 2692
Size: 5693
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1981 2013 4832 4864 1981
Size: 32 2819 32 2810
Fragments arranged by size:
2819 2810 32 32
PpuMI rG'GwC_Cy
Cuts at: 158 174 2122 158
Size: 16 1948 3729
Fragments arranged by size:
3729 1948 16
PsiI TTA'TAA
Cuts at: 2246 2524 2246
Size: 278 5415
PstI C_TGCA'G
Cuts at: 871 1776 2411 2740 3425 871
Size: 905 635 329 685 3139
Fragments arranged by size:
3139 905 685 635 329
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5693
PvuII CAG'CTG
Cuts at: 1321 3118 3478 1321
Size: 1797 360 3536
Fragments arranged by size:
3536 1797 360
RsrII CG'GwC_CG
Cuts at: 3888 3888
Size: 5693
SacI G_AGCT'C
Cuts at: 1687 2707 1687
Size: 1020 4673
SalI G'TCGA_C
Cuts at: 677 2730 677
Size: 2053 3640
SapI GCTCTTCn'nnn_
Cuts at: 2063 3716 3926 2063
Size: 1653 210 3830
Fragments arranged by size:
3830 1653 210
SbfI CC_TGCA'GG
Cuts at: 2740 2740
Size: 5693
ScaI AGT'ACT
Cuts at: 5210 5210
Size: 5693
SexAI A'CCwGG_T
Cuts at: 2124 2124
Size: 5693
SfcI C'TryA_G
Cuts at: 33 791 867 1548 1772 2407 2736 3421 4414
Size: 758 76 681 224 635 329 685 993
Cuts at: 4414 4605 33
Size: 191 1121
Fragments arranged by size:
1121 993 758 685 681 635 329 224
191 76
SfoI GGC'GCC
Cuts at: 3125 3373 3125
Size: 248 5445
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5693
SmaI CCC'GGG
Cuts at: 2715 2715
Size: 5693
SmlI C'TyrA_G
Cuts at: 2848 3763 4255 4517 2848
Size: 915 492 262 4024
Fragments arranged by size:
4024 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5693
SphI G_CATG'C
Cuts at: 2746 3246 2746
Size: 500 5193
SspI AAT'ATT
Cuts at: 467 467
Size: 5693
StyI C'CwwG_G
Cuts at: 1922 2827 3181 1922
Size: 905 354 4434
Fragments arranged by size:
4434 905 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3276 3409 3868 4077 3276
Size: 133 459 209 4892
Fragments arranged by size:
4892 459 209 133
TatI w'GTAC_w
Cuts at: 421 5208 421
Size: 4787 906
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5210 5242 5210
Size: 32 5661
Tth111I GACn'n_nGTC
Cuts at: 2089 3490 2089
Size: 1401 4292
XbaI T'CTAG_A
Cuts at: 1624 2724 1624
Size: 1100 4593
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 137 3188 137
Size: 3051 2642
XmaI C'CCGG_G
Cuts at: 2713 2713
Size: 5693
XmnI GAAnn'nnTTC
Cuts at: 1328 1328
Size: 5693
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PacI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI
BglI BmtI BseRI BsiWI BsmBI BssHII BstZ17I Bsu36I
BtgI ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI StuI SwaI XhoI ZraI