Restriction map for
pF1KB5696
(Circular) (Six-base) MAPSORT of: pF1KB5696.seq Check: 4697 from: 1 to: 3848
pF1KB5696 3848 bp
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AccI GT'mk_AC
Cuts at: 886 886
Size: 3848
Acc65I G'GTAC_C
Cuts at: 864 864
Size: 3848
AclI AA'CG_TT
Cuts at: 3688 3688
Size: 3848
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1673 2105 2852 3120 839
Size: 834 432 747 268 1567
Fragments arranged by size:
1567 834 747 432 268
AflIII A'CryG_T
Cuts at: 1200 2304 3281 1200
Size: 1104 977 1767
Fragments arranged by size:
1767 1104 977
AgeI A'CCGG_T
Cuts at: 2236 2236
Size: 3848
AlwNI CAG_nnn'CTG
Cuts at: 1153 2720 1153
Size: 1567 2281
ApaLI G'TGCA_C
Cuts at: 2618 2618
Size: 3848
ApoI r'AATT_y
Cuts at: 852 3201 3457 852
Size: 2349 256 1243
Fragments arranged by size:
2349 1243 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3848
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
AvaI C'yCGr_G
Cuts at: 344 868 344
Size: 524 3324
BamHI G'GATC_C
Cuts at: 873 873
Size: 3848
BanI G'GyrC_C
Cuts at: 864 1278 1526 1561 864
Size: 414 248 35 3151
Fragments arranged by size:
3151 414 248 35
BanII G_rGCy'C
Cuts at: 270 862 1892 270
Size: 592 1030 2226
Fragments arranged by size:
2226 1030 592
BbeI G_GCGC'C
Cuts at: 1282 1530 1282
Size: 248 3600
BbsI GAAGACnn'nnnn_
Cuts at: 447 447
Size: 3848
BbvCI CC'TCA_GC
Cuts at: 497 497
Size: 3848
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 325 359 325
Size: 34 3814
BciVI GTATCCnnnnn_n'
Cuts at: 792 1746 2507 3848 792
Size: 954 761 1341 792
Fragments arranged by size:
1341 954 792 761
BclI T'GATC_A
Cuts at: 1368 1368
Size: 3848
BglI GCCn_nnn'nGGC
Cuts at: 142 151 142
Size: 9 3839
BglII A'GATC_T
Cuts at: 1363 3400 1363
Size: 2037 1811
BlpI GC'TnA_GC
Cuts at: 155 960 155
Size: 805 3043
Bme1580I G_kGCm'C
Cuts at: 1473 1566 2622 1473
Size: 93 1056 2699
Fragments arranged by size:
2699 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 619 1060 1141 1476 3620 619
Size: 441 81 335 2144 847
Fragments arranged by size:
2144 847 441 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 3848
Bpu10I CC'TnA_GC
Cuts at: 497 497
Size: 3848
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 1024 1903 2395 2693 279
Size: 745 879 492 298 1434
Fragments arranged by size:
1434 879 745 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3552 3552
Size: 3848
BsaAI yAC'GTr
Cuts at: 1201 1201
Size: 3848
BsaBI GATnn'nnATC
Cuts at: 6 1362 3399 6
Size: 1356 2037 455
Fragments arranged by size:
2037 1356 455
BsaHI Gr'CG_yC
Cuts at: 1279 1527 2229 3725 1279
Size: 248 702 1496 1402
Fragments arranged by size:
1496 1402 702 248
BsaWI w'CCGG_w
Cuts at: 1037 1558 2236 2510 2657 3068 1037
Size: 521 678 274 147 411 1817
Fragments arranged by size:
1817 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 238 238
Size: 3848
BseYI C'CCAG_C
Cuts at: 1273 2031 2608 3104 1273
Size: 758 577 496 2017
Fragments arranged by size:
2017 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1390 1436 2644 104
Size: 1286 46 1208 1308
Fragments arranged by size:
1308 1286 1208 46
BsiHKAI G_wGCw'C
Cuts at: 862 1640 1830 2622 862
Size: 778 190 792 2088
Fragments arranged by size:
2088 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 297 361 803 297
Size: 64 442 3342
Fragments arranged by size:
3342 442 64
Bsp1286I G_dGCh'C
Cuts at: 270 862 1473 1566 1640 1830 1892 2622 270
Size: 592 611 93 74 190 62 730 1496
Fragments arranged by size:
1496 730 611 592 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3068 3068
Size: 3848
BspHI T'CATG_A
Cuts at: 3024 3024
Size: 3848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 898 1112 1414 1795 898
Size: 214 302 381 2951
Fragments arranged by size:
2951 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2140 2194 3678 3843 50
Size: 2090 54 1484 165 55
Fragments arranged by size:
2090 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 396 704 1760 396
Size: 308 1056 2484
Fragments arranged by size:
2484 1056 308
BsrFI r'CCGG_y
Cuts at: 1846 2027 2236 1846
Size: 181 209 3458
Fragments arranged by size:
3458 209 181
BssSI C'ACGA_G
Cuts at: 2119 2477 2119
Size: 358 3490
BstAPI GCAn_nnn'nTGC
Cuts at: 1225 1225
Size: 3848
BstBI TT'CG_AA
Cuts at: 2209 2209
Size: 3848
BstYI r'GATC_y
Cuts at: 1 873 1340 1363 1698 1944 2956 3042 3054
Size: 872 467 23 335 246 1012 86 12
Cuts at: 3054 3400 1
Size: 346 449
Fragments arranged by size:
1012 872 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 3848
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1798 1979 2142 1798
Size: 181 163 3504
Fragments arranged by size:
3504 181 163
BtsI GCAGTG_nn'
Cuts at: 1091 1091
Size: 3848
DraI TTT'AAA
Cuts at: 847 847
Size: 3848
DrdI GACnn_nn'nnGTC
Cuts at: 1554 2412 1554
Size: 858 2990
EaeI y'GGCC_r
Cuts at: 143 1433 1607 1998 2025 3370 143
Size: 1290 174 391 27 1345 621
Fragments arranged by size:
1345 1290 621 391 174 27
EagI C'GGCC_G
Cuts at: 1433 1433
Size: 3848
EarI CTCTTCn'nnn_
Cuts at: 725 1871 2081 725
Size: 1146 210 2492
Fragments arranged by size:
2492 1146 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2056 2366 2512 3658 2056
Size: 310 146 1146 2246
Fragments arranged by size:
2246 1146 310 146
EcoICRI GAG'CTC
Cuts at: 860 860
Size: 3848
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1673 2105 2852 3120 839
Size: 834 432 747 268 1567
Fragments arranged by size:
1567 834 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 987 987
Size: 3848
EcoRI G'AATT_C
Cuts at: 852 852
Size: 3848
EcoRV GAT'ATC
Cuts at: 384 384
Size: 3848
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1867 1867
Size: 3848
FspI TGC'GCA
Cuts at: 1629 1629
Size: 3848
HaeII r_GCGC'y
Cuts at: 1282 1530 2552 3531 1282
Size: 248 1022 979 1599
Fragments arranged by size:
1599 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 519 551 1690 1720 1722 1752 385
Size: 32 102 32 1139 30 2 30 2481
Fragments arranged by size:
2481 1139 102 32 32 30 30 2
HincII GTy'rAC
Cuts at: 887 887
Size: 3848
HindIII A'AGCT_T
Cuts at: 560 560
Size: 3848
KasI G'GCGC_C
Cuts at: 1278 1526 1278
Size: 248 3600
KpnI G_GTAC'C
Cuts at: 868 868
Size: 3848
MluI A'CGCG_T
Cuts at: 3281 3281
Size: 3848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2519 2703 2519
Size: 184 3664
MscI TGG'CCA
Cuts at: 1609 1609
Size: 3848
MslI CAynn'nnrTG
Cuts at: 1837 1964 1837
Size: 127 3721
MspA1I CmG'CkG
Cuts at: 508 959 1273 1633 2646 2891 508
Size: 451 314 360 1013 245 1465
Fragments arranged by size:
1465 1013 451 360 314 245
NaeI GCC'GGC
Cuts at: 2029 2029
Size: 3848
NarI GG'CG_CC
Cuts at: 1279 1527 1279
Size: 248 3600
NcoI C'CATG_G
Cuts at: 107 107
Size: 3848
NgoMIV G'CCGG_C
Cuts at: 2027 2027
Size: 3848
NspI r_CATG'y
Cuts at: 901 1401 2308 901
Size: 500 907 2441
Fragments arranged by size:
2441 907 500
PciI A'CATG_T
Cuts at: 2304 2304
Size: 3848
PmeI GTTT'AAAC
Cuts at: 847 847
Size: 3848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2987 3019 2987
Size: 32 3816
PsiI TTA'TAA
Cuts at: 251 251
Size: 3848
PstI C_TGCA'G
Cuts at: 895 1580 895
Size: 685 3163
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3848
PvuII CAG'CTG
Cuts at: 1273 1633 1273
Size: 360 3488
RsrII CG'GwC_CG
Cuts at: 2043 2043
Size: 3848
SacI G_AGCT'C
Cuts at: 862 862
Size: 3848
SalI G'TCGA_C
Cuts at: 885 885
Size: 3848
SapI GCTCTTCn'nnn_
Cuts at: 1871 2081 1871
Size: 210 3638
SbfI CC_TGCA'GG
Cuts at: 895 895
Size: 3848
ScaI AGT'ACT
Cuts at: 3365 3365
Size: 3848
SfcI C'TryA_G
Cuts at: 33 891 1576 2569 2760 33
Size: 858 685 993 191 1121
Fragments arranged by size:
1121 993 858 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 142 142
Size: 3848
SfoI GGC'GCC
Cuts at: 1280 1528 1280
Size: 248 3600
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3848
SmaI CCC'GGG
Cuts at: 870 870
Size: 3848
SmlI C'TyrA_G
Cuts at: 294 344 1003 1918 2410 2672 294
Size: 50 659 915 492 262 1470
Fragments arranged by size:
1470 915 659 492 262 50
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3848
SphI G_CATG'C
Cuts at: 901 1401 901
Size: 500 3348
StyI C'CwwG_G
Cuts at: 107 804 982 1336 107
Size: 697 178 354 2619
Fragments arranged by size:
2619 697 354 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1431 1564 2023 2232 1431
Size: 133 459 209 3047
Fragments arranged by size:
3047 459 209 133
TatI w'GTAC_w
Cuts at: 396 3363 396
Size: 2967 881
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 3816
Tth111I GACn'n_nGTC
Cuts at: 611 1645 611
Size: 1034 2814
XbaI T'CTAG_A
Cuts at: 879 879
Size: 3848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1343 1343
Size: 3848
XhoI C'TCGA_G
Cuts at: 344 344
Size: 3848
XmaI C'CCGG_G
Cuts at: 868 868
Size: 3848
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PciI PmeI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BfrBI BmgBI BmtI BpmI
BsaXI BsgI BsiWI BsmI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
StuI SwaI XmnI ZraI