Restriction map for
pF1KB5697
(Circular) (Six-base) MAPSORT of: pF1KB5697.seq Check: 3607 from: 1 to: 5093
pF1KB5697 5093 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 1676 2131 1676
Size: 455 4638
Acc65I G'GTAC_C
Cuts at: 1760 2109 1760
Size: 349 4744
AclI AA'CG_TT
Cuts at: 4933 4933
Size: 5093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 986 2041 2062 2918 3350 3703 4365 337
Size: 649 1055 21 856 432 353 662 1065
Fragments arranged by size:
1065 1055 856 662 649 432 353 21
AflIII A'CryG_T
Cuts at: 2445 4245 4526 2445
Size: 1800 281 3012
Fragments arranged by size:
3012 1800 281
AgeI A'CCGG_T
Cuts at: 1577 1577
Size: 5093
AlwNI CAG_nnn'CTG
Cuts at: 703 2398 3836 703
Size: 1695 1438 1960
Fragments arranged by size:
1960 1695 1438
ApaI G_GGCC'C
Cuts at: 1365 1495 1625 1365
Size: 130 130 4833
Fragments arranged by size:
4833 130 130
ApaLI G'TGCA_C
Cuts at: 3931 3931
Size: 5093
ApoI r'AATT_y
Cuts at: 1867 2097 4446 4702 1867
Size: 230 2349 256 2258
Fragments arranged by size:
2349 2258 256 230
AseI AT'TA_AT
Cuts at: 20 20
Size: 5093
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
AvaI C'yCGr_G
Cuts at: 125 2113 125
Size: 1988 3105
BamHI G'GATC_C
Cuts at: 1902 2118 1902
Size: 216 4877
BanI G'GyrC_C
Cuts at: 1060 1144 1371 1468 1760 2008 2109 2523 2771
Size: 84 227 97 292 248 101 414 248
Cuts at: 2771 2806 1060
Size: 35 3347
Fragments arranged by size:
3347 414 292 248 248 227 101 97
84 35
BanII G_rGCy'C
Cuts at: 1365 1439 1495 1625 1655 2107 3137 1365
Size: 74 56 130 30 452 1030 3321
Fragments arranged by size:
3321 1030 452 130 74 56 30
BbeI G_GCGC'C
Cuts at: 2527 2775 2527
Size: 248 4845
BbsI GAAGACnn'nnnn_
Cuts at: 1149 1849 1149
Size: 700 4393
BciVI GTATCCnnnnn_n'
Cuts at: 643 2991 4047 5093 643
Size: 2348 1056 1046 643
Fragments arranged by size:
2348 1056 1046 643
BclI T'GATC_A
Cuts at: 617 2613 617
Size: 1996 3097
BglI GCCn_nnn'nGGC
Cuts at: 112 1369 1620 112
Size: 1257 251 3585
Fragments arranged by size:
3585 1257 251
BglII A'GATC_T
Cuts at: 1508 1559 2608 4645 1508
Size: 51 1049 2037 1956
Fragments arranged by size:
2037 1956 1049 51
BlpI GC'TnA_GC
Cuts at: 178 2205 178
Size: 2027 3066
Bme1580I G_kGCm'C
Cuts at: 1006 1149 1365 1495 1625 2718 2811 3935 1006
Size: 143 216 130 130 1093 93 1124 2164
Fragments arranged by size:
2164 1124 1093 216 143 130 130 93
BmrI ACTGGGnnnn_n'
Cuts at: 2305 2386 2721 4865 2305
Size: 81 335 2144 2533
Fragments arranged by size:
2533 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3460 3460
Size: 5093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 579 1360 1771 1848 1880 1893 2047 579
Size: 781 411 77 32 13 154 3625
Fragments arranged by size:
3625 781 411 154 77 32 13
Bpu10I CC'TnA_GC
Cuts at: 1649 1649
Size: 5093
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3148 3862 4160 2269
Size: 879 714 298 3202
Fragments arranged by size:
3202 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 385 1102 4797 385
Size: 717 3695 681
Fragments arranged by size:
3695 717 681
BsaAI yAC'GTr
Cuts at: 1606 2446 1606
Size: 840 4253
BsaBI GATnn'nnATC
Cuts at: 6 2607 4644 6
Size: 2601 2037 455
Fragments arranged by size:
2601 2037 455
BsaHI Gr'CG_yC
Cuts at: 1600 2524 2772 3474 4970 1600
Size: 924 248 702 1496 1723
Fragments arranged by size:
1723 1496 924 702 248
BsaWI w'CCGG_w
Cuts at: 1577 2282 2803 3481 3892 4039 4313 1577
Size: 705 521 678 411 147 274 2357
Fragments arranged by size:
2357 705 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1744 1774 1744
Size: 30 5063
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1708 1708
Size: 5093
BseYI C'CCAG_C
Cuts at: 632 1488 2518 3276 3941 4349 632
Size: 856 1030 758 665 408 1376
Fragments arranged by size:
1376 1030 856 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 831 1005 1179 831
Size: 174 174 4745
Fragments arranged by size:
4745 174 174
BsiEI CG_ry'CG
Cuts at: 104 861 1080 1209 1260 2635 2681 3911 104
Size: 757 219 129 51 1375 46 1230 1286
Fragments arranged by size:
1375 1286 1230 757 219 129 51 46
BsiHKAI G_wGCw'C
Cuts at: 886 1346 1439 1655 2107 2885 3075 3935 886
Size: 460 93 216 452 778 190 860 2044
Fragments arranged by size:
2044 860 778 460 452 216 190 93
BsmI GAATG_Cn'
Cuts at: 1427 1944 1427
Size: 517 4576
Bsp1286I G_dGCh'C
Cuts at: 886 1006 1149 1346 1365 1439 1495 1625 1655
Size: 120 143 197 19 74 56 130 30
Cuts at: 1655 2107 2718 2811 2885 3075 3137 3935 886
Size: 452 611 93 74 190 62 798 2044
Fragments arranged by size:
2044 798 611 452 197 190 143 130
120 93 74 74 62 56 30 19
BspHI T'CATG_A
Cuts at: 614 3525 614
Size: 2911 2182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1982 2143 2357 2659 3040 1982
Size: 161 214 302 381 4035
Fragments arranged by size:
4035 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 3385 3439 4923 5088 50
Size: 3335 54 1484 165 55
Fragments arranged by size:
3335 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3005 3005
Size: 5093
BsrFI r'CCGG_y
Cuts at: 856 1204 1471 1577 3091 3272 856
Size: 348 267 106 1514 181 2677
Fragments arranged by size:
2677 1514 348 267 181 106
BssHII G'CGCG_C
Cuts at: 131 131
Size: 5093
BssSI C'ACGA_G
Cuts at: 542 1303 1315 3364 4072 542
Size: 761 12 2049 708 1563
Fragments arranged by size:
2049 1563 761 708 12
BstAPI GCAn_nnn'nTGC
Cuts at: 2470 2470
Size: 5093
BstBI TT'CG_AA
Cuts at: 3454 3454
Size: 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 1321 1338 1357 1749 1321
Size: 17 19 392 4665
Fragments arranged by size:
4665 392 19 17
BstYI r'GATC_y
Cuts at: 1 1508 1547 1559 1730 1902 2118 2585 2608
Size: 1507 39 12 171 172 216 467 23
Cuts at: 2608 2943 3189 3495 3507 3593 4645 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1507 1052 467 449 335 306 246 216
172 171 86 39 23 12 12
BstZ17I GTA'TAC
Cuts at: 1677 1677
Size: 5093
Bsu36I CC'TnA_GG
Cuts at: 148 148
Size: 5093
BtgI C'CryG_G
Cuts at: 107 950 1331 1551 1616 107
Size: 843 381 220 65 3584
Fragments arranged by size:
3584 843 381 220 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 239 578 659 881 1524 1908 3043 3224 3387
Size: 339 81 222 643 384 1135 181 163
Cuts at: 3387 239
Size: 1945
Fragments arranged by size:
1945 1135 643 384 339 222 181 163
81
BtsI GCAGTG_nn'
Cuts at: 263 574 1218 2336 263
Size: 311 644 1118 3020
Fragments arranged by size:
3020 1118 644 311
DraI TTT'AAA
Cuts at: 2092 2092
Size: 5093
DrdI GACnn_nn'nnGTC
Cuts at: 1116 2799 4143 1116
Size: 1683 1344 2066
Fragments arranged by size:
2066 1683 1344
EaeI y'GGCC_r
Cuts at: 428 627 858 1206 1323 1634 2678 2852 3243
Size: 199 231 348 117 311 1044 174 391
Cuts at: 3243 3270 4615 428
Size: 27 1345 906
Fragments arranged by size:
1345 1044 906 391 348 311 231 199
174 117 27
EagI C'GGCC_G
Cuts at: 858 1206 2678 858
Size: 348 1472 3273
Fragments arranged by size:
3273 1472 348
EarI CTCTTCn'nnn_
Cuts at: 2038 3116 3326 2038
Size: 1078 210 3805
Fragments arranged by size:
3805 1078 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1605 3301 4043 4189 4903 1605
Size: 1696 742 146 714 1795
Fragments arranged by size:
1795 1696 742 714 146
EcoICRI GAG'CTC
Cuts at: 1437 1653 2105 1437
Size: 216 452 4425
Fragments arranged by size:
4425 452 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 579 986 1360 1771 1848 1880 1893 2041
Size: 242 407 374 411 77 32 13 148
Cuts at: 2041 2047 2062 2918 3350 3703 4365 337
Size: 6 15 856 432 353 662 1065
Fragments arranged by size:
1065 856 662 432 411 407 374 353
242 148 77 32 15 13 6
EcoNI CCTnn'n_nnAGG
Cuts at: 1283 1283
Size: 5093
EcoO109I rG'GnC_Cy
Cuts at: 142 1361 1362 1445 1492 1621 1622 1683 2232
Size: 1219 1 83 47 129 1 61 549
Cuts at: 2232 142
Size: 3003
Fragments arranged by size:
3003 1219 549 129 83 61 47 1
1
EcoRI G'AATT_C
Cuts at: 2097 2097
Size: 5093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 831 939 1113 1287 3112 831
Size: 108 174 174 1825 2812
Fragments arranged by size:
2812 1825 174 174 108
FspI TGC'GCA
Cuts at: 2874 2874
Size: 5093
HaeII r_GCGC'y
Cuts at: 118 157 1205 2527 2775 4005 4776 118
Size: 39 1048 1322 248 1230 771 435
Fragments arranged by size:
1322 1230 1048 771 435 248 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 354 386 2935 2965 2967 2997 354
Size: 32 2549 30 2 30 2450
Fragments arranged by size:
2549 2450 32 30 30 2
HincII GTy'rAC
Cuts at: 2132 2132
Size: 5093
KasI G'GCGC_C
Cuts at: 2523 2771 2523
Size: 248 4845
KpnI G_GTAC'C
Cuts at: 1764 2113 1764
Size: 349 4744
MluI A'CGCG_T
Cuts at: 4526 4526
Size: 5093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2096 3852 4036 2096
Size: 1756 184 3153
Fragments arranged by size:
3153 1756 184
MscI TGG'CCA
Cuts at: 430 1325 1636 2854 430
Size: 895 311 1218 2669
Fragments arranged by size:
2669 1218 895 311
MslI CAynn'nnrTG
Cuts at: 644 728 3082 3209 644
Size: 84 2354 127 2528
Fragments arranged by size:
2528 2354 127 84
MspA1I CmG'CkG
Cuts at: 632 845 1019 1052 2204 2518 2878 3662 3907
Size: 213 174 33 1152 314 360 784 245
Cuts at: 3907 632
Size: 1818
Fragments arranged by size:
1818 1152 784 360 314 245 213 174
33
NaeI GCC'GGC
Cuts at: 1206 3274 1206
Size: 2068 3025
NarI GG'CG_CC
Cuts at: 2524 2772 2524
Size: 248 4845
NcoI C'CATG_G
Cuts at: 107 950 1331 1616 107
Size: 843 381 285 3584
Fragments arranged by size:
3584 843 381 285
NgoMIV G'CCGG_C
Cuts at: 1204 3272 1204
Size: 2068 3025
NspI r_CATG'y
Cuts at: 2146 2646 4249 2146
Size: 500 1603 2990
Fragments arranged by size:
2990 1603 500
PasI CC'CwG_GG
Cuts at: 1357 1357
Size: 5093
PciI A'CATG_T
Cuts at: 4245 4245
Size: 5093
PflMI CCAn_nnn'nTGG
Cuts at: 703 1332 1643 1809 703
Size: 629 311 166 3987
Fragments arranged by size:
3987 629 311 166
PfoI T'CCnGG_A
Cuts at: 1655 1655
Size: 5093
PmeI GTTT'AAAC
Cuts at: 2092 2092
Size: 5093
PmlI CAC'GTG
Cuts at: 1606 1606
Size: 5093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3539 3571 3539
Size: 32 5061
PpuMI rG'GwC_Cy
Cuts at: 142 142
Size: 5093
PspOMI G'GGCC_C
Cuts at: 1361 1491 1621 1361
Size: 130 130 4833
Fragments arranged by size:
4833 130 130
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 793 825 1282 1314 793
Size: 32 457 32 4572
Fragments arranged by size:
4572 457 32 32
PstI C_TGCA'G
Cuts at: 1979 2140 2825 1979
Size: 161 685 4247
Fragments arranged by size:
4247 685 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5093
PvuII CAG'CTG
Cuts at: 845 1019 2518 2878 845
Size: 174 1499 360 3060
Fragments arranged by size:
3060 1499 360 174
RsrII CG'GwC_CG
Cuts at: 3288 3288
Size: 5093
SacI G_AGCT'C
Cuts at: 1439 1655 2107 1439
Size: 216 452 4425
Fragments arranged by size:
4425 452 216
SalI G'TCGA_C
Cuts at: 2130 2130
Size: 5093
SanDI GG'GwC_CC
Cuts at: 142 142
Size: 5093
SapI GCTCTTCn'nnn_
Cuts at: 2038 3116 3326 2038
Size: 1078 210 3805
Fragments arranged by size:
3805 1078 210
SbfI CC_TGCA'GG
Cuts at: 1979 2140 1979
Size: 161 4932
ScaI AGT'ACT
Cuts at: 802 4610 802
Size: 3808 1285
SfcI C'TryA_G
Cuts at: 33 1975 2136 2821 3789 3980 33
Size: 1942 161 685 968 191 1146
Fragments arranged by size:
1942 1146 968 685 191 161
SfoI GGC'GCC
Cuts at: 2525 2773 2525
Size: 248 4845
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5093
SmaI CCC'GGG
Cuts at: 127 2115 127
Size: 1988 3105
SmlI C'TyrA_G
Cuts at: 2248 3163 3877 4139 2248
Size: 915 714 262 3202
Fragments arranged by size:
3202 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5093
SphI G_CATG'C
Cuts at: 2146 2646 2146
Size: 500 4593
StyI C'CwwG_G
Cuts at: 107 290 749 950 1284 1331 1464 1616 2227
Size: 183 459 201 334 47 133 152 611
Cuts at: 2227 2581 107
Size: 354 2619
Fragments arranged by size:
2619 611 459 354 334 201 183 152
133 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1389 2676 2809 3268 3477 1389
Size: 1287 133 459 209 3005
Fragments arranged by size:
3005 1287 459 209 133
TatI w'GTAC_w
Cuts at: 800 836 4608 800
Size: 36 3772 1285
Fragments arranged by size:
3772 1285 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4610 4642 4610
Size: 32 5061
Tth111I GACn'n_nGTC
Cuts at: 2890 2890
Size: 5093
XbaI T'CTAG_A
Cuts at: 2124 2124
Size: 5093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1485 2588 1485
Size: 1103 3990
XmaI C'CCGG_G
Cuts at: 125 2113 125
Size: 1988 3105
XmnI GAAnn'nnTTC
Cuts at: 274 274
Size: 5093
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI
BsiWI BsmBI BspEI BsrGI BstEII ClaI CspCI DraIII
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI