Restriction map for
pF1KB5702
(Circular) (Six-base) MAPSORT of: pF1KB5702.seq Check: 3499 from: 1 to: 5831
pF1KB5702 5831 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 548 1245 1808 2869 548
Size: 697 563 1061 3510
Fragments arranged by size:
3510 1061 697 563
Acc65I G'GTAC_C
Cuts at: 2847 2847
Size: 5831
AclI AA'CG_TT
Cuts at: 758 5671 758
Size: 4913 918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 978 2228 3656 4088 4441 5103 674
Size: 304 1250 1428 432 353 662 1402
Fragments arranged by size:
1428 1402 1250 662 432 353 304
AfeI AGC'GCT
Cuts at: 1006 1006
Size: 5831
AflIII A'CryG_T
Cuts at: 1081 3183 4983 5264 1081
Size: 2102 1800 281 1648
Fragments arranged by size:
2102 1800 1648 281
AleI CACnn'nnGTG
Cuts at: 920 920
Size: 5831
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2421 2421
Size: 5831
AlwNI CAG_nnn'CTG
Cuts at: 1137 1146 1609 2433 3136 4574 1137
Size: 9 463 824 703 1438 2394
Fragments arranged by size:
2394 1438 824 703 463 9
ApaI G_GGCC'C
Cuts at: 248 248
Size: 5831
ApaLI G'TGCA_C
Cuts at: 4669 4669
Size: 5831
ApoI r'AATT_y
Cuts at: 318 2835 5184 5440 318
Size: 2517 2349 256 709
Fragments arranged by size:
2517 2349 709 256
AseI AT'TA_AT
Cuts at: 20 1502 20
Size: 1482 4349
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
AvaI C'yCGr_G
Cuts at: 491 1757 2114 2297 2851 491
Size: 1266 357 183 554 3471
Fragments arranged by size:
3471 1266 554 357 183
BamHI G'GATC_C
Cuts at: 147 2365 2856 147
Size: 2218 491 3122
Fragments arranged by size:
3122 2218 491
BanI G'GyrC_C
Cuts at: 2694 2847 3261 3509 3544 2694
Size: 153 414 248 35 4981
Fragments arranged by size:
4981 414 248 153 35
BanII G_rGCy'C
Cuts at: 248 2845 3875 248
Size: 2597 1030 2204
Fragments arranged by size:
2597 2204 1030
BbeI G_GCGC'C
Cuts at: 3265 3513 3265
Size: 248 5583
BbsI GAAGACnn'nnnn_
Cuts at: 1234 2090 1234
Size: 856 4975
BbvCI CC'TCA_GC
Cuts at: 1900 2357 2797 1900
Size: 457 440 4934
Fragments arranged by size:
4934 457 440
BciVI GTATCCnnnnn_n'
Cuts at: 3729 4785 5831 3729
Size: 1056 1046 3729
Fragments arranged by size:
3729 1056 1046
BclI T'GATC_A
Cuts at: 720 3351 720
Size: 2631 3200
BglI GCCn_nnn'nGGC
Cuts at: 820 1063 1414 820
Size: 243 351 5237
Fragments arranged by size:
5237 351 243
BglII A'GATC_T
Cuts at: 3346 5383 3346
Size: 2037 3794
BlpI GC'TnA_GC
Cuts at: 2129 2943 2129
Size: 814 5017
Bme1580I G_kGCm'C
Cuts at: 248 2115 3456 3549 4673 248
Size: 1867 1341 93 1124 1406
Fragments arranged by size:
1867 1406 1341 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 3043 3124 3459 5603 3043
Size: 81 335 2144 3271
Fragments arranged by size:
3271 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 517 4198 517
Size: 3681 2150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1042
Size: 5831
Bpu10I CC'TnA_GC
Cuts at: 1900 2357 2797 1900
Size: 457 440 4934
Fragments arranged by size:
4934 457 440
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 1008 3007 3886 4600 4898 349
Size: 659 1999 879 714 298 1282
Fragments arranged by size:
1999 1282 879 714 659 298
BsaI GGTCTCn'nnnn_
Cuts at: 5535 5535
Size: 5831
BsaAI yAC'GTr
Cuts at: 1770 2778 3184 1770
Size: 1008 406 4417
Fragments arranged by size:
4417 1008 406
BsaBI GATnn'nnATC
Cuts at: 6 3345 5382 6
Size: 3339 2037 455
Fragments arranged by size:
3339 2037 455
BsaHI Gr'CG_yC
Cuts at: 3262 3510 4212 5708 3262
Size: 248 702 1496 3385
Fragments arranged by size:
3385 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3020 3541 4219 4630 4777 5051 3020
Size: 521 678 411 147 274 3800
Fragments arranged by size:
3800 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 343 373 343
Size: 30 5801
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 385 941 944 947 1015 1039 2029 385
Size: 556 3 3 68 24 990 4187
Fragments arranged by size:
4187 990 556 68 24 3 3
BseYI C'CCAG_C
Cuts at: 1916 3256 4014 4679 5087 1916
Size: 1340 758 665 408 2660
Fragments arranged by size:
2660 1340 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1062 1493 2527 2617 2809 1062
Size: 431 1034 90 192 4084
Fragments arranged by size:
4084 1034 431 192 90
BsiEI CG_ry'CG
Cuts at: 104 3373 3419 4649 104
Size: 3269 46 1230 1286
Fragments arranged by size:
3269 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2845 3623 3813 4673 2845
Size: 778 190 860 4003
Fragments arranged by size:
4003 860 778 190
BsmI GAATG_Cn'
Cuts at: 609 2592 609
Size: 1983 3848
Bsp1286I G_dGCh'C
Cuts at: 248 2115 2845 3456 3549 3623 3813 3875 4673
Size: 1867 730 611 93 74 190 62 798
Cuts at: 4673 248
Size: 1406
Fragments arranged by size:
1867 1406 798 730 611 190 93 74
62
BspHI T'CATG_A
Cuts at: 4263 4263
Size: 5831
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1154 2441 2453 2881 3095 3397 3778 1154
Size: 1287 12 428 214 302 381 3207
Fragments arranged by size:
3207 1287 428 381 302 214 12
BsrBI CCG'CTC
Cuts at: 50 4123 4177 5661 5826 50
Size: 4073 54 1484 165 55
Fragments arranged by size:
4073 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 618 1173 1688 2241 2393 2403 3743 618
Size: 555 515 553 152 10 1340 2706
Fragments arranged by size:
2706 1340 555 553 515 152 10
BsrFI r'CCGG_y
Cuts at: 3829 4010 3829
Size: 181 5650
BssSI C'ACGA_G
Cuts at: 4102 4810 4102
Size: 708 5123
BstAPI GCAn_nnn'nTGC
Cuts at: 1146 2433 3208 1146
Size: 1287 775 3769
Fragments arranged by size:
3769 1287 775
BstBI TT'CG_AA
Cuts at: 399 1458 4192 399
Size: 1059 2734 2038
Fragments arranged by size:
2734 2038 1059
BstYI r'GATC_y
Cuts at: 1 147 1560 2365 2856 3323 3346 3681 3927
Size: 146 1413 805 491 467 23 335 246
Cuts at: 3927 4233 4245 4331 5383 1
Size: 306 12 86 1052 449
Fragments arranged by size:
1413 1052 805 491 467 449 335 306
246 146 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1493 2012 1493
Size: 519 5312
BtgI C'CryG_G
Cuts at: 1889 1922 1889
Size: 33 5798
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2439 3781 3962 4125 2439
Size: 1342 181 163 4145
Fragments arranged by size:
4145 1342 181 163
BtsI GCAGTG_nn'
Cuts at: 890 1997 3074 890
Size: 1107 1077 3647
Fragments arranged by size:
3647 1107 1077
ClaI AT'CG_AT
Cuts at: 1943 1943
Size: 5831
DraI TTT'AAA
Cuts at: 1569 2830 1569
Size: 1261 4570
DrdI GACnn_nn'nnGTC
Cuts at: 3537 4881 3537
Size: 1344 4487
EaeI y'GGCC_r
Cuts at: 711 1064 2103 2171 2252 2385 3416 3590 3981
Size: 353 1039 68 81 133 1031 174 391
Cuts at: 3981 4008 5353 711
Size: 27 1345 1189
Fragments arranged by size:
1345 1189 1039 1031 391 353 174 133
81 68 27
EagI C'GGCC_G
Cuts at: 3416 3416
Size: 5831
EarI CTCTTCn'nnn_
Cuts at: 253 462 2713 3854 4064 253
Size: 209 2251 1141 210 2020
Fragments arranged by size:
2251 2020 1141 210 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1117 4039 4781 4927 5641 1117
Size: 2922 742 146 714 1307
Fragments arranged by size:
2922 1307 742 714 146
EcoICRI GAG'CTC
Cuts at: 2843 2843
Size: 5831
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 674 978 1042 2228 3656 4088 4441 5103 674
Size: 304 64 1186 1428 432 353 662 1402
Fragments arranged by size:
1428 1402 1186 662 432 353 304 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2563 2563
Size: 5831
EcoO109I rG'GnC_Cy
Cuts at: 244 245 831 1134 1143 2006 2430 2793 2970
Size: 1 586 303 9 863 424 363 177
Cuts at: 2970 244
Size: 3105
Fragments arranged by size:
3105 863 586 424 363 303 177 9
1
EcoRI G'AATT_C
Cuts at: 2835 2835
Size: 5831
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3850 3850
Size: 5831
FspI TGC'GCA
Cuts at: 3612 3612
Size: 5831
HaeII r_GCGC'y
Cuts at: 1008 2552 3265 3513 4743 5514 1008
Size: 1544 713 248 1230 771 1325
Fragments arranged by size:
1544 1325 1230 771 713 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 156 188 439 471 892 924 2112 2144 2350
Size: 32 251 32 421 32 1188 32 206
Cuts at: 2350 2382 3673 3703 3705 3735 156
Size: 32 1291 30 2 30 2252
Fragments arranged by size:
2252 1291 1188 421 251 206 32 32
32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2870 2870
Size: 5831
HindIII A'AGCT_T
Cuts at: 1450 2380 1450
Size: 930 4901
KasI G'GCGC_C
Cuts at: 3261 3509 3261
Size: 248 5583
KpnI G_GTAC'C
Cuts at: 2851 2851
Size: 5831
MluI A'CGCG_T
Cuts at: 1081 5264 1081
Size: 4183 1648
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 173 1871 2000 4590 4774 173
Size: 1698 129 2590 184 1230
Fragments arranged by size:
2590 1698 1230 184 129
MscI TGG'CCA
Cuts at: 713 2105 2173 2254 3592 713
Size: 1392 68 81 1338 2952
Fragments arranged by size:
2952 1392 1338 81 68
MslI CAynn'nnrTG
Cuts at: 803 920 1855 2245 2272 3820 3947 803
Size: 117 935 390 27 1548 127 2687
Fragments arranged by size:
2687 1548 935 390 127 117 27
MspA1I CmG'CkG
Cuts at: 567 849 1732 2094 2475 2942 3256 3616 4400
Size: 282 883 362 381 467 314 360 784
Cuts at: 4400 4645 567
Size: 245 1753
Fragments arranged by size:
1753 883 784 467 381 362 360 314
282 245
NaeI GCC'GGC
Cuts at: 4012 4012
Size: 5831
NarI GG'CG_CC
Cuts at: 3262 3510 3262
Size: 248 5583
NcoI C'CATG_G
Cuts at: 1922 1922
Size: 5831
NdeI CA'TA_TG
Cuts at: 945 945
Size: 5831
NgoMIV G'CCGG_C
Cuts at: 4010 4010
Size: 5831
NspI r_CATG'y
Cuts at: 791 1387 2884 3384 4987 791
Size: 596 1497 500 1603 1635
Fragments arranged by size:
1635 1603 1497 596 500
PciI A'CATG_T
Cuts at: 4983 4983
Size: 5831
PflMI CCAn_nnn'nTGG
Cuts at: 288 1923 288
Size: 1635 4196
PfoI T'CCnGG_A
Cuts at: 1289 1973 2180 2368 1289
Size: 684 207 188 4752
Fragments arranged by size:
4752 684 207 188
PmeI GTTT'AAAC
Cuts at: 2830 2830
Size: 5831
PmlI CAC'GTG
Cuts at: 2778 2778
Size: 5831
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 252 284 4277 4309 252
Size: 32 3993 32 1774
Fragments arranged by size:
3993 1774 32 32
PpuMI rG'GwC_Cy
Cuts at: 831 1143 2006 2430 831
Size: 312 863 424 4232
Fragments arranged by size:
4232 863 424 312
PshAI GACnn'nnGTC
Cuts at: 115 2071 115
Size: 1956 3875
PspOMI G'GGCC_C
Cuts at: 244 244
Size: 5831
PspXI vC'TCGA_Gb
Cuts at: 491 491
Size: 5831
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 380 412 1439 1471 380
Size: 32 1027 32 4740
Fragments arranged by size:
4740 1027 32 32
PstI C_TGCA'G
Cuts at: 432 735 752 854 1142 2488 2878 3563 432
Size: 303 17 102 288 1346 390 685 2700
Fragments arranged by size:
2700 1346 685 390 303 288 102 17
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5831
PvuII CAG'CTG
Cuts at: 567 849 1732 2094 2475 3256 3616 567
Size: 282 883 362 381 781 360 2782
Fragments arranged by size:
2782 883 781 381 362 360 282
RsrII CG'GwC_CG
Cuts at: 4026 4026
Size: 5831
SacI G_AGCT'C
Cuts at: 2845 2845
Size: 5831
SalI G'TCGA_C
Cuts at: 2868 2868
Size: 5831
SapI GCTCTTCn'nnn_
Cuts at: 462 3854 4064 462
Size: 3392 210 2229
Fragments arranged by size:
3392 2229 210
SbfI CC_TGCA'GG
Cuts at: 1142 2878 1142
Size: 1736 4095
ScaI AGT'ACT
Cuts at: 5348 5348
Size: 5831
SfcI C'TryA_G
Cuts at: 33 386 428 731 748 793 850 1138 2484
Size: 353 42 303 17 45 57 288 1346
Cuts at: 2484 2874 3559 4527 4718 33
Size: 390 685 968 191 1146
Fragments arranged by size:
1346 1146 968 685 390 353 303 288
191 57 45 42 17
SfoI GGC'GCC
Cuts at: 3263 3511 3263
Size: 248 5583
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5831
SmaI CCC'GGG
Cuts at: 1759 2116 2853 1759
Size: 357 737 4737
Fragments arranged by size:
4737 737 357
SmlI C'TyrA_G
Cuts at: 364 491 987 2986 3901 4615 4877 364
Size: 127 496 1999 915 714 262 1318
Fragments arranged by size:
1999 1318 915 714 496 262 127
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5831
SphI G_CATG'C
Cuts at: 791 2884 3384 791
Size: 2093 500 3238
Fragments arranged by size:
3238 2093 500
SrfI GCCC'GGGC
Cuts at: 2116 2116
Size: 5831
StuI AGG'CCT
Cuts at: 1014 1014
Size: 5831
StyI C'CwwG_G
Cuts at: 1922 2965 3319 1922
Size: 1043 354 4434
Fragments arranged by size:
4434 1043 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3414 3547 4006 4215 3414
Size: 133 459 209 5030
Fragments arranged by size:
5030 459 209 133
TatI w'GTAC_w
Cuts at: 1157 5346 1157
Size: 4189 1642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5348 5380 5348
Size: 32 5799
Tth111I GACn'n_nGTC
Cuts at: 3628 3628
Size: 5831
XbaI T'CTAG_A
Cuts at: 159 2862 159
Size: 2703 3128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3326 3326
Size: 5831
XhoI C'TCGA_G
Cuts at: 491 491
Size: 5831
XmaI C'CCGG_G
Cuts at: 1757 2114 2851 1757
Size: 357 737 4737
Fragments arranged by size:
4737 737 357
XmnI GAAnn'nnTTC
Cuts at: 395 395
Size: 5831
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BspEI
BsrGI BssHII BstEII BstXI BstZ17I CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PsiI SacII SanDI SexAI SfiI SgrAI
SnaBI SspI SwaI ZraI