Restriction map for
pF1KB5705
(Circular) (Six-base) MAPSORT of: pF1KB5705.seq Check: 4677 from: 1 to: 5345
pF1KB5705 5345 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AatII G_ACGT'C
Cuts at: 304 304
Size: 5345
AccI GT'mk_AC
Cuts at: 1856 1883 2383 1856
Size: 27 500 4818
Fragments arranged by size:
4818 500 27
Acc65I G'GTAC_C
Cuts at: 191 2361 191
Size: 2170 3175
AclI AA'CG_TT
Cuts at: 5185 5185
Size: 5345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3602 3955 4617 3170
Size: 432 353 662 3898
Fragments arranged by size:
3898 662 432 353
AfeI AGC'GCT
Cuts at: 1185 1185
Size: 5345
AflIII A'CryG_T
Cuts at: 2697 4497 4778 2697
Size: 1800 281 3264
Fragments arranged by size:
3264 1800 281
AhdI GACnn_n'nnGTC
Cuts at: 1890 1890
Size: 5345
AleI CACnn'nnGTG
Cuts at: 1720 1720
Size: 5345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 571 943 1456 2132 571
Size: 372 513 676 3784
Fragments arranged by size:
3784 676 513 372
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 145 177 145
Size: 32 5313
AlwNI CAG_nnn'CTG
Cuts at: 165 179 270 675 762 860 882 2262 2650
Size: 14 91 405 87 98 22 1380 388
Cuts at: 2650 4088 165
Size: 1438 1422
Fragments arranged by size:
1438 1422 1380 405 388 98 91 87
22 14
ApaI G_GGCC'C
Cuts at: 396 1840 1982 396
Size: 1444 142 3759
Fragments arranged by size:
3759 1444 142
ApaLI G'TGCA_C
Cuts at: 4183 4183
Size: 5345
ApoI r'AATT_y
Cuts at: 1561 2349 4698 4954 1561
Size: 788 2349 256 1952
Fragments arranged by size:
2349 1952 788 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5345
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
AvaI C'yCGr_G
Cuts at: 604 2365 604
Size: 1761 3584
BamHI G'GATC_C
Cuts at: 2370 2370
Size: 5345
BanI G'GyrC_C
Cuts at: 191 381 1233 2361 2775 3023 3058 191
Size: 190 852 1128 414 248 35 2478
Fragments arranged by size:
2478 1128 852 414 248 190 35
BanII G_rGCy'C
Cuts at: 396 895 1523 1840 1982 2359 3389 396
Size: 499 628 317 142 377 1030 2352
Fragments arranged by size:
2352 1030 628 499 377 317 142
BbeI G_GCGC'C
Cuts at: 2779 3027 2779
Size: 248 5097
BbsI GAAGACnn'nnnn_
Cuts at: 1518 1518
Size: 5345
BbvCI CC'TCA_GC
Cuts at: 559 559
Size: 5345
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2255 2289 2255
Size: 34 5311
BciVI GTATCCnnnnn_n'
Cuts at: 3243 4299 5345 3243
Size: 1056 1046 3243
Fragments arranged by size:
3243 1056 1046
BclI T'GATC_A
Cuts at: 2865 2865
Size: 5345
BglI GCCn_nnn'nGGC
Cuts at: 1217 1835 1217
Size: 618 4727
BglII A'GATC_T
Cuts at: 2860 4897 2860
Size: 2037 3308
BlpI GC'TnA_GC
Cuts at: 1187 2457 1187
Size: 1270 4075
Bme1580I G_kGCm'C
Cuts at: 202 396 1362 1840 1982 2970 3063 4187 202
Size: 194 966 478 142 988 93 1124 1360
Fragments arranged by size:
1360 1124 988 966 478 194 142 93
BmrI ACTGGGnnnn_n'
Cuts at: 2178 2557 2638 2973 5117 2178
Size: 379 81 335 2144 2406
Fragments arranged by size:
2406 2144 379 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2089 3712 2089
Size: 1623 3722
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 796 2320 751
Size: 45 1524 3776
Fragments arranged by size:
3776 1524 45
Bpu10I CC'TnA_GC
Cuts at: 559 559
Size: 5345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2521 3400 4114 4412 1027
Size: 1494 879 714 298 1960
Fragments arranged by size:
1960 1494 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 772 5049 772
Size: 4277 1068
BsaAI yAC'GTr
Cuts at: 2698 2698
Size: 5345
BsaBI GATnn'nnATC
Cuts at: 6 2859 4896 6
Size: 2853 2037 455
Fragments arranged by size:
2853 2037 455
BsaHI Gr'CG_yC
Cuts at: 301 323 2776 3024 3726 5222 301
Size: 22 2453 248 702 1496 424
Fragments arranged by size:
2453 1496 702 424 248 22
BsaWI w'CCGG_w
Cuts at: 1230 1859 2534 3055 3733 4144 4291 4565 1230
Size: 629 675 521 678 411 147 274 2010
Fragments arranged by size:
2010 678 675 629 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1838 1868 2120 2150 1838
Size: 30 252 30 5033
Fragments arranged by size:
5033 252 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1294 1294
Size: 5345
BseYI C'CCAG_C
Cuts at: 1465 2770 3528 4193 4601 1465
Size: 1305 758 665 408 2209
Fragments arranged by size:
2209 1305 758 665 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 205 734 1055 1217 205
Size: 529 321 162 4333
Fragments arranged by size:
4333 529 321 162
BsiEI CG_ry'CG
Cuts at: 104 1030 1602 2887 2933 4163 104
Size: 926 572 1285 46 1230 1286
Fragments arranged by size:
1286 1285 1230 926 572 46
BsiHKAI G_wGCw'C
Cuts at: 1037 1523 2359 3137 3327 4187 1037
Size: 486 836 778 190 860 2195
Fragments arranged by size:
2195 860 836 778 486 190
BsmI GAATG_Cn'
Cuts at: 990 990
Size: 5345
BsmBI CGTCTCn'nnnn_
Cuts at: 330 493 330
Size: 163 5182
Bsp1286I G_dGCh'C
Cuts at: 202 396 895 1037 1362 1523 1840 1982 2359
Size: 194 499 142 325 161 317 142 377
Cuts at: 2359 2970 3063 3137 3327 3389 4187 202
Size: 611 93 74 190 62 798 1360
Fragments arranged by size:
1360 798 611 499 377 325 317 194
190 161 142 142 93 74 62
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 5345
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1385 2395 2609 2911 3292 1385
Size: 1010 214 302 381 3438
Fragments arranged by size:
3438 1010 381 302 214
BsrBI CCG'CTC
Cuts at: 50 936 972 3637 3691 5175 5340 50
Size: 886 36 2665 54 1484 165 55
Fragments arranged by size:
2665 1484 886 165 55 54 36
BsrDI GCAATG_nn'
Cuts at: 377 2109 3257 377
Size: 1732 1148 2465
Fragments arranged by size:
2465 1732 1148
BsrFI r'CCGG_y
Cuts at: 293 1207 3343 3524 293
Size: 914 2136 181 2114
Fragments arranged by size:
2136 2114 914 181
BsrGI T'GTAC_A
Cuts at: 1274 1475 2051 1274
Size: 201 576 4568
Fragments arranged by size:
4568 576 201
BssHII G'CGCG_C
Cuts at: 1282 1282
Size: 5345
BssSI C'ACGA_G
Cuts at: 1059 3616 4324 1059
Size: 2557 708 2080
Fragments arranged by size:
2557 2080 708
BstAPI GCAn_nnn'nTGC
Cuts at: 179 646 741 860 882 2312 2722 179
Size: 467 95 119 22 1430 410 2802
Fragments arranged by size:
2802 1430 467 410 119 95 22
BstBI TT'CG_AA
Cuts at: 3706 3706
Size: 5345
BstXI CCAn_nnnn'nTGG
Cuts at: 1255 1722 1848 1921 1255
Size: 467 126 73 4679
Fragments arranged by size:
4679 467 126 73
BstYI r'GATC_y
Cuts at: 1 2370 2837 2860 3195 3441 3747 3759 3845
Size: 2369 467 23 335 246 306 12 86
Cuts at: 3845 4897 1
Size: 1052 449
Fragments arranged by size:
2369 1052 467 449 335 306 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1793 1793
Size: 5345
BtgI C'CryG_G
Cuts at: 107 1088 1950 2091 107
Size: 981 862 141 3361
Fragments arranged by size:
3361 981 862 141
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3295 3476 3639 3295
Size: 181 163 5001
Fragments arranged by size:
5001 181 163
BtsI GCAGTG_nn'
Cuts at: 331 739 826 880 1048 1067 1460 1733 2310
Size: 408 87 54 168 19 393 273 577
Cuts at: 2310 2588 331
Size: 278 3088
Fragments arranged by size:
3088 577 408 393 278 273 168 87
54 19
DraI TTT'AAA
Cuts at: 2319 2344 2319
Size: 25 5320
DrdI GACnn_nn'nnGTC
Cuts at: 3051 4395 3051
Size: 1344 4001
EaeI y'GGCC_r
Cuts at: 435 503 618 973 1085 1599 1824 1850 1935
Size: 68 115 355 112 514 225 26 85
Cuts at: 1935 2280 2930 3104 3495 3522 4867 435
Size: 345 650 174 391 27 1345 913
Fragments arranged by size:
1345 913 650 514 391 355 345 225
174 115 112 85 68 27 26
EagI C'GGCC_G
Cuts at: 1599 2930 1599
Size: 1331 4014
EarI CTCTTCn'nnn_
Cuts at: 2080 3368 3578 2080
Size: 1288 210 3847
Fragments arranged by size:
3847 1288 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3553 4295 4441 5155 3553
Size: 742 146 714 3743
Fragments arranged by size:
3743 742 714 146
EcoICRI GAG'CTC
Cuts at: 1521 2357 1521
Size: 836 4509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 751 796 2320 3170 3602 3955 4617 751
Size: 45 1524 850 432 353 662 1479
Fragments arranged by size:
1524 1479 850 662 432 353 45
EcoNI CCTnn'n_nnAGG
Cuts at: 364 1105 364
Size: 741 4604
EcoO109I rG'GnC_Cy
Cuts at: 392 408 696 726 1098 1374 1837 1978 1979
Size: 16 288 30 372 276 463 141 1
Cuts at: 1979 2259 2484 392
Size: 280 225 3253
Fragments arranged by size:
3253 463 372 288 280 276 225 141
30 16 1
EcoRI G'AATT_C
Cuts at: 1561 2349 1561
Size: 788 4557
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3364 3364
Size: 5345
FspI TGC'GCA
Cuts at: 919 1174 3126 919
Size: 255 1952 3138
Fragments arranged by size:
3138 1952 255
FspAI rTGC'GCAy
Cuts at: 1174 1174
Size: 5345
HaeII r_GCGC'y
Cuts at: 600 1067 1114 1187 1407 1713 2779 3027 4257
Size: 467 47 73 220 306 1066 248 1230
Cuts at: 4257 5028 600
Size: 771 917
Fragments arranged by size:
1230 1066 917 771 467 306 248 220
73 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1019 1051 1876 1908 3187 3217 3219 3249 1019
Size: 32 825 32 1279 30 2 30 3115
Fragments arranged by size:
3115 1279 825 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1884 2384 1884
Size: 500 4845
KasI G'GCGC_C
Cuts at: 2775 3023 2775
Size: 248 5097
KpnI G_GTAC'C
Cuts at: 195 2365 195
Size: 2170 3175
MfeI C'AATT_G
Cuts at: 644 1508 644
Size: 864 4481
MluI A'CGCG_T
Cuts at: 4778 4778
Size: 5345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4104 4288 4104
Size: 184 5161
MscI TGG'CCA
Cuts at: 1087 2282 3106 1087
Size: 1195 824 3326
Fragments arranged by size:
3326 1195 824
MslI CAynn'nnrTG
Cuts at: 983 1253 1720 1750 2158 3334 3461 983
Size: 270 467 30 408 1176 127 2867
Fragments arranged by size:
2867 1176 467 408 270 127 30
MspA1I CmG'CkG
Cuts at: 633 675 819 993 1598 1704 2456 2770 3130
Size: 42 144 174 605 106 752 314 360
Cuts at: 3130 3914 4159 633
Size: 784 245 1819
Fragments arranged by size:
1819 784 752 605 360 314 245 174
144 106 42
NaeI GCC'GGC
Cuts at: 295 3526 295
Size: 3231 2114
NarI GG'CG_CC
Cuts at: 2776 3024 2776
Size: 248 5097
NcoI C'CATG_G
Cuts at: 107 2091 107
Size: 1984 3361
NdeI CA'TA_TG
Cuts at: 1263 1263
Size: 5345
NgoMIV G'CCGG_C
Cuts at: 293 3524 293
Size: 3231 2114
NotI GC'GGCC_GC
Cuts at: 1599 1599
Size: 5345
NspI r_CATG'y
Cuts at: 930 2398 2898 4501 930
Size: 1468 500 1603 1774
Fragments arranged by size:
1774 1603 1468 500
PciI A'CATG_T
Cuts at: 4497 4497
Size: 5345
PflMI CCAn_nnn'nTGG
Cuts at: 150 1989 2248 2289 150
Size: 1839 259 41 3206
Fragments arranged by size:
3206 1839 259 41
PfoI T'CCnGG_A
Cuts at: 965 965
Size: 5345
PmeI GTTT'AAAC
Cuts at: 2344 2344
Size: 5345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1645 1677 1786 1818 3791 3823 1645
Size: 32 109 32 1973 32 3167
Fragments arranged by size:
3167 1973 109 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 408 726 1374 408
Size: 318 648 4379
Fragments arranged by size:
4379 648 318
PspOMI G'GGCC_C
Cuts at: 392 1836 1978 392
Size: 1444 142 3759
Fragments arranged by size:
3759 1444 142
PspXI vC'TCGA_Gb
Cuts at: 604 604
Size: 5345
PstI C_TGCA'G
Cuts at: 338 596 680 746 865 1709 2238 2392 3077
Size: 258 84 66 119 844 529 154 685
Cuts at: 3077 338
Size: 2606
Fragments arranged by size:
2606 844 685 529 258 154 119 84
66
PvuI CG_AT'CG
Cuts at: 104 1030 104
Size: 926 4419
PvuII CAG'CTG
Cuts at: 633 675 819 993 1704 2770 3130 633
Size: 42 144 174 711 1066 360 2848
Fragments arranged by size:
2848 1066 711 360 174 144 42
RsrII CG'GwC_CG
Cuts at: 3540 3540
Size: 5345
SacI G_AGCT'C
Cuts at: 1523 2359 1523
Size: 836 4509
SalI G'TCGA_C
Cuts at: 1882 2382 1882
Size: 500 4845
SapI GCTCTTCn'nnn_
Cuts at: 3368 3578 3368
Size: 210 5135
SbfI CC_TGCA'GG
Cuts at: 2392 2392
Size: 5345
ScaI AGT'ACT
Cuts at: 4862 4862
Size: 5345
SfcI C'TryA_G
Cuts at: 33 334 592 676 742 861 1705 2234 2388
Size: 301 258 84 66 119 844 529 154
Cuts at: 2388 3073 4041 4232 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 844 685 529 301 258 191
154 119 84 66
SfoI GGC'GCC
Cuts at: 2777 3025 2777
Size: 248 5097
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5345
SmaI CCC'GGG
Cuts at: 2367 2367
Size: 5345
SmlI C'TyrA_G
Cuts at: 604 1006 2500 3415 4129 4391 604
Size: 402 1494 915 714 262 1558
Fragments arranged by size:
1558 1494 915 714 402 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5345
SphI G_CATG'C
Cuts at: 2398 2898 2398
Size: 500 4845
StuI AGG'CCT
Cuts at: 493 961 493
Size: 468 4877
StyI C'CwwG_G
Cuts at: 107 203 1841 2091 2479 2833 107
Size: 96 1638 250 388 354 2619
Fragments arranged by size:
2619 1638 388 354 250 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2928 3061 3520 3729 2928
Size: 133 459 209 4544
Fragments arranged by size:
4544 459 209 133
TatI w'GTAC_w
Cuts at: 1274 1475 2051 4860 1274
Size: 201 576 2809 1759
Fragments arranged by size:
2809 1759 576 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 947 979 1212 1244 1316 1348 2120 2152 4862
Size: 32 233 32 72 32 772 32 2710
Cuts at: 4862 4894 947
Size: 32 1398
Fragments arranged by size:
2710 1398 772 233 72 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 3142 3142
Size: 5345
XbaI T'CTAG_A
Cuts at: 2376 2376
Size: 5345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1926 2840 1926
Size: 914 4431
XhoI C'TCGA_G
Cuts at: 604 604
Size: 5345
XmaI C'CCGG_G
Cuts at: 2365 2365
Size: 5345
XmnI GAAnn'nnTTC
Cuts at: 343 343
Size: 5345
ZraI GAC'GTC
Cuts at: 302 302
Size: 5345
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NotI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AgeI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BspEI BstEII BstZ17I ClaI CspCI DraIII
EcoRV FseI HindIII HpaI NheI NruI NsiI PacI
PasI PmlI PshAI PsiI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI