Restriction map for
pF1KB5710
(Circular) (Six-base) MAPSORT of: pF1KB5710.seq Check: 5415 from: 1 to: 5264
pF1KB5710 5264 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1086 1200 1086
Size: 114 5150
AccI GT'mk_AC
Cuts at: 2302 2302
Size: 5264
Acc65I G'GTAC_C
Cuts at: 793 2280 793
Size: 1487 3777
AclI AA'CG_TT
Cuts at: 5104 5104
Size: 5264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 343 436 803 849 1006 1483 1721 3089
Size: 15 93 367 46 157 477 238 1368
Cuts at: 3089 3521 4268 4536 328
Size: 432 747 268 1056
Fragments arranged by size:
1368 1056 747 477 432 367 268 238
157 93 46 15
AfeI AGC'GCT
Cuts at: 685 685
Size: 5264
AflIII A'CryG_T
Cuts at: 2616 3720 4697 2616
Size: 1104 977 3183
Fragments arranged by size:
3183 1104 977
AgeI A'CCGG_T
Cuts at: 3652 3652
Size: 5264
AhdI GACnn_n'nnGTC
Cuts at: 591 624 591
Size: 33 5231
AleI CACnn'nnGTG
Cuts at: 1438 1438
Size: 5264
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 767 1948 2137 767
Size: 1181 189 3894
Fragments arranged by size:
3894 1181 189
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2003 2035 2003
Size: 32 5232
AlwNI CAG_nnn'CTG
Cuts at: 198 738 954 984 1221 2099 2187 2569 4136
Size: 540 216 30 237 878 88 382 1567
Cuts at: 4136 198
Size: 1326
Fragments arranged by size:
1567 1326 878 540 382 237 216 88
30
ApaI G_GGCC'C
Cuts at: 1653 1653
Size: 5264
ApaLI G'TGCA_C
Cuts at: 4034 4034
Size: 5264
ApoI r'AATT_y
Cuts at: 2268 4617 4873 2268
Size: 2349 256 2659
Fragments arranged by size:
2659 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
AvaI C'yCGr_G
Cuts at: 2284 2284
Size: 5264
BamHI G'GATC_C
Cuts at: 1422 2289 1422
Size: 867 4397
BanI G'GyrC_C
Cuts at: 793 1061 1971 2148 2280 2694 2942 2977 793
Size: 268 910 177 132 414 248 35 3080
Fragments arranged by size:
3080 910 414 268 248 177 132 35
BanII G_rGCy'C
Cuts at: 440 566 1177 1478 1653 2278 3308 440
Size: 126 611 301 175 625 1030 2396
Fragments arranged by size:
2396 1030 625 611 301 175 126
BbeI G_GCGC'C
Cuts at: 1065 2152 2698 2946 1065
Size: 1087 546 248 3383
Fragments arranged by size:
3383 1087 546 248
BbsI GAAGACnn'nnnn_
Cuts at: 1007 1007
Size: 5264
BbvCI CC'TCA_GC
Cuts at: 659 1078 659
Size: 419 4845
BciVI GTATCCnnnnn_n'
Cuts at: 3162 3923 5264 3162
Size: 761 1341 3162
Fragments arranged by size:
3162 1341 761
BclI T'GATC_A
Cuts at: 2784 2784
Size: 5264
BglI GCCn_nnn'nGGC
Cuts at: 973 1070 1172 1252 973
Size: 97 102 80 4985
Fragments arranged by size:
4985 102 97 80
BglII A'GATC_T
Cuts at: 1761 2779 4816 1761
Size: 1018 2037 2209
Fragments arranged by size:
2209 2037 1018
BlpI GC'TnA_GC
Cuts at: 125 2376 125
Size: 2251 3013
Bme1580I G_kGCm'C
Cuts at: 1653 1974 2889 2982 4038 1653
Size: 321 915 93 1056 2879
Fragments arranged by size:
2879 1056 915 321 93
BmrI ACTGGGnnnn_n'
Cuts at: 2157 2476 2557 2892 5036 2157
Size: 319 81 335 2144 2385
Fragments arranged by size:
2385 2144 335 319 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1359 3631 1359
Size: 2272 2992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 718 862 892 934 1057 1129 1171 1213
Size: 465 144 30 42 123 72 42 42
Cuts at: 1213 1306 1525 1594 1750 1786 1822 1961 2056
Size: 93 219 69 156 36 36 139 95
Cuts at: 2056 253
Size: 3461
Fragments arranged by size:
3461 465 219 156 144 139 123 95
93 72 69 42 42 42 36 36
30
Bpu10I CC'TnA_GC
Cuts at: 199 659 1078 1477 199
Size: 460 419 399 3986
Fragments arranged by size:
3986 460 419 399
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 3319 3811 4109 2440
Size: 879 492 298 3595
Fragments arranged by size:
3595 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 143 808 4968 143
Size: 665 4160 439
Fragments arranged by size:
4160 665 439
BsaAI yAC'GTr
Cuts at: 1911 2617 1911
Size: 706 4558
BsaBI GATnn'nnATC
Cuts at: 6 2778 4815 6
Size: 2772 2037 455
Fragments arranged by size:
2772 2037 455
BsaHI Gr'CG_yC
Cuts at: 1062 2149 2220 2695 2943 3645 5141 1062
Size: 1087 71 475 248 702 1496 1185
Fragments arranged by size:
1496 1185 1087 702 475 248 71
BsaWI w'CCGG_w
Cuts at: 941 2453 2974 3652 3926 4073 4484 941
Size: 1512 521 678 274 147 411 1721
Fragments arranged by size:
1721 1512 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1279 1309 1312 1342 1279
Size: 30 3 30 5201
Fragments arranged by size:
5201 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 667 676 739 871 1255 1378 1570 1810 1855
Size: 9 63 132 384 123 192 240 45
Cuts at: 1855 1906 2155 667
Size: 51 249 3776
Fragments arranged by size:
3776 384 249 240 192 132 123 63
51 45 9
BseYI C'CCAG_C
Cuts at: 462 836 1027 1187 1379 2134 2689 3447 4024
Size: 374 191 160 192 755 555 758 577
Cuts at: 4024 4520 462
Size: 496 1206
Fragments arranged by size:
1206 758 755 577 555 496 374 192
191 160
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1234 1542 1234
Size: 308 4956
BsiEI CG_ry'CG
Cuts at: 104 1538 1542 2806 2852 4060 104
Size: 1434 4 1264 46 1208 1308
Fragments arranged by size:
1434 1308 1264 1208 46 4
BsiHKAI G_wGCw'C
Cuts at: 440 566 1478 2278 3056 3246 4038 440
Size: 126 912 800 778 190 792 1666
Fragments arranged by size:
1666 912 800 792 778 190 126
BsmI GAATG_Cn'
Cuts at: 756 1279 756
Size: 523 4741
BsmBI CGTCTCn'nnnn_
Cuts at: 179 595 1477 1600 179
Size: 416 882 123 3843
Fragments arranged by size:
3843 882 416 123
Bsp1286I G_dGCh'C
Cuts at: 440 566 1177 1478 1653 1974 2278 2889 2982
Size: 126 611 301 175 321 304 611 93
Cuts at: 2982 3056 3246 3308 4038 440
Size: 74 190 62 730 1666
Fragments arranged by size:
1666 730 611 611 321 304 301 190
175 126 93 74 62
BspEI T'CCGG_A
Cuts at: 4484 4484
Size: 5264
BspHI T'CATG_A
Cuts at: 512 566 1752 4440 512
Size: 54 1186 2688 1336
Fragments arranged by size:
2688 1336 1186 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 623 1086 1200 1673 2314 2528 2830 3211 623
Size: 463 114 473 641 214 302 381 2676
Fragments arranged by size:
2676 641 473 463 381 302 214 114
BsrBI CCG'CTC
Cuts at: 50 702 768 1171 1323 1338 1548 2145 3556
Size: 652 66 403 152 15 210 597 1411
Cuts at: 3556 3610 5094 5259 50
Size: 54 1484 165 55
Fragments arranged by size:
1484 1411 652 597 403 210 165 152
66 55 54 15
BsrDI GCAATG_nn'
Cuts at: 3176 3176
Size: 5264
BsrFI r'CCGG_y
Cuts at: 776 1058 1123 1616 3262 3443 3652 776
Size: 282 65 493 1646 181 209 2388
Fragments arranged by size:
2388 1646 493 282 209 181 65
BssHII G'CGCG_C
Cuts at: 1182 1589 1591 1182
Size: 407 2 4855
Fragments arranged by size:
4855 407 2
BssSI C'ACGA_G
Cuts at: 3535 3893 3535
Size: 358 4906
BstAPI GCAn_nnn'nTGC
Cuts at: 711 1221 1857 2641 711
Size: 510 636 784 3334
Fragments arranged by size:
3334 784 636 510
BstBI TT'CG_AA
Cuts at: 3625 3625
Size: 5264
BstXI CCAn_nnnn'nTGG
Cuts at: 114 1440 114
Size: 1326 3938
BstYI r'GATC_y
Cuts at: 1 285 693 1422 1460 1761 2289 2756 2779
Size: 284 408 729 38 301 528 467 23
Cuts at: 2779 3114 3360 4372 4458 4470 4816 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 729 528 467 449 408 346 335
301 284 246 86 38 23 12
Bsu36I CC'TnA_GG
Cuts at: 191 1993 191
Size: 1802 3462
BtgI C'CryG_G
Cuts at: 1390 1439 1596 1886 1940 2205 1390
Size: 49 157 290 54 265 4449
Fragments arranged by size:
4449 290 265 157 54 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3214 3395 3558 3214
Size: 181 163 4920
Fragments arranged by size:
4920 181 163
BtsI GCAGTG_nn'
Cuts at: 806 2507 806
Size: 1701 3563
DraI TTT'AAA
Cuts at: 2263 2263
Size: 5264
DrdI GACnn_nn'nnGTC
Cuts at: 2970 3828 2970
Size: 858 4406
EaeI y'GGCC_r
Cuts at: 1125 1535 1957 2849 3023 3414 3441 4786 1125
Size: 410 422 892 174 391 27 1345 1603
Fragments arranged by size:
1603 1345 892 422 410 391 174 27
EagI C'GGCC_G
Cuts at: 1535 2849 1535
Size: 1314 3950
EarI CTCTTCn'nnn_
Cuts at: 380 3287 3497 380
Size: 2907 210 2147
Fragments arranged by size:
2907 2147 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1348 1848 3472 3782 3928 5074 1348
Size: 500 1624 310 146 1146 1538
Fragments arranged by size:
1624 1538 1146 500 310 146
EcoICRI GAG'CTC
Cuts at: 438 564 1476 2276 438
Size: 126 912 800 3426
Fragments arranged by size:
3426 912 800 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 328 343 436 718 803 849 862 892
Size: 75 15 93 282 85 46 13 30
Cuts at: 892 934 1006 1057 1129 1171 1213 1306 1483
Size: 42 72 51 72 42 42 93 177
Cuts at: 1483 1525 1594 1721 1750 1786 1822 1961 2056
Size: 42 69 127 29 36 36 139 95
Cuts at: 2056 3089 3521 4268 4536 253
Size: 1033 432 747 268 981
Fragments arranged by size:
1033 981 747 432 282 268 177 139
127 95 93 93 85 75 72 72
69 51 46 42 42 42 42 36
36 30 29 15 13
EcoNI CCTnn'n_nnAGG
Cuts at: 649 721 967 649
Size: 72 246 4946
Fragments arranged by size:
4946 246 72
EcoO109I rG'GnC_Cy
Cuts at: 195 292 981 1662 2403 195
Size: 97 689 681 741 3056
Fragments arranged by size:
3056 741 689 681 97
EcoRI G'AATT_C
Cuts at: 2268 2268
Size: 5264
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 348 3283 348
Size: 2935 2329
FseI GG_CCGG'CC
Cuts at: 780 780
Size: 5264
FspI TGC'GCA
Cuts at: 3045 3045
Size: 5264
HaeII r_GCGC'y
Cuts at: 687 897 955 1065 1569 2152 2698 2946 3968
Size: 210 58 110 504 583 546 248 1022
Cuts at: 3968 4947 687
Size: 979 1004
Fragments arranged by size:
1022 1004 979 583 546 504 248 210
110 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 577 609 610 642 1414 1446 3106 3136 3138
Size: 32 1 32 772 32 1660 30 2
Cuts at: 3138 3168 577
Size: 30 2673
Fragments arranged by size:
2673 1660 772 32 32 32 30 30
2 1
HincII GTy'rAC
Cuts at: 1400 2303 1400
Size: 903 4361
HindIII A'AGCT_T
Cuts at: 1747 1747
Size: 5264
KasI G'GCGC_C
Cuts at: 1061 2148 2694 2942 1061
Size: 1087 546 248 3383
Fragments arranged by size:
3383 1087 546 248
KpnI G_GTAC'C
Cuts at: 797 2284 797
Size: 1487 3777
MfeI C'AATT_G
Cuts at: 847 847
Size: 5264
MluI A'CGCG_T
Cuts at: 4697 4697
Size: 5264
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1583 2052 3935 4119 1583
Size: 469 1883 184 2728
Fragments arranged by size:
2728 1883 469 184
MscI TGG'CCA
Cuts at: 3025 3025
Size: 5264
MslI CAynn'nnrTG
Cuts at: 112 1438 1930 3253 3380 112
Size: 1326 492 1323 127 1996
Fragments arranged by size:
1996 1326 1323 492 127
MspA1I CmG'CkG
Cuts at: 206 456 708 840 1035 1107 1191 1203 1233
Size: 250 252 132 195 72 84 12 30
Cuts at: 1233 1383 1392 1503 1956 1963 2067 2184 2375
Size: 150 9 111 453 7 104 117 191
Cuts at: 2375 2689 3049 4062 4307 206
Size: 314 360 1013 245 1163
Fragments arranged by size:
1163 1013 453 360 314 252 250 245
195 191 150 132 117 111 104 84
72 30 12 9 7
NaeI GCC'GGC
Cuts at: 778 1060 1125 3445 778
Size: 282 65 2320 2597
Fragments arranged by size:
2597 2320 282 65
NarI GG'CG_CC
Cuts at: 1062 2149 2695 2943 1062
Size: 1087 546 248 3383
Fragments arranged by size:
3383 1087 546 248
NcoI C'CATG_G
Cuts at: 1886 1940 1886
Size: 54 5210
NgoMIV G'CCGG_C
Cuts at: 776 1058 1123 3443 776
Size: 282 65 2320 2597
Fragments arranged by size:
2597 2320 282 65
NspI r_CATG'y
Cuts at: 541 2317 2817 3724 541
Size: 1776 500 907 2081
Fragments arranged by size:
2081 1776 907 500
PciI A'CATG_T
Cuts at: 3720 3720
Size: 5264
PflMI CCAn_nnn'nTGG
Cuts at: 1602 1602
Size: 5264
PmeI GTTT'AAAC
Cuts at: 2263 2263
Size: 5264
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4403 4435 4403
Size: 32 5232
PpuMI rG'GwC_Cy
Cuts at: 195 292 1662 195
Size: 97 1370 3797
Fragments arranged by size:
3797 1370 97
PspOMI G'GGCC_C
Cuts at: 1649 1649
Size: 5264
PstI C_TGCA'G
Cuts at: 938 1226 1388 1565 1670 1682 1850 2311 2996
Size: 288 162 177 105 12 168 461 685
Cuts at: 2996 938
Size: 3206
Fragments arranged by size:
3206 685 461 288 177 168 162 105
12
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5264
PvuII CAG'CTG
Cuts at: 456 708 840 1035 1107 1191 1383 1503 1963
Size: 252 132 195 72 84 192 120 460
Cuts at: 1963 2689 3049 456
Size: 726 360 2671
Fragments arranged by size:
2671 726 460 360 252 195 192 132
120 84 72
RsrII CG'GwC_CG
Cuts at: 3459 3459
Size: 5264
SacI G_AGCT'C
Cuts at: 440 566 1478 2278 440
Size: 126 912 800 3426
Fragments arranged by size:
3426 912 800 126
SacII CC_GC'GG
Cuts at: 1393 1393
Size: 5264
SalI G'TCGA_C
Cuts at: 2301 2301
Size: 5264
SapI GCTCTTCn'nnn_
Cuts at: 380 3287 3497 380
Size: 2907 210 2147
Fragments arranged by size:
2907 2147 210
SbfI CC_TGCA'GG
Cuts at: 1682 2311 1682
Size: 629 4635
ScaI AGT'ACT
Cuts at: 352 4781 352
Size: 4429 835
SexAI A'CCwGG_T
Cuts at: 148 148
Size: 5264
SfcI C'TryA_G
Cuts at: 33 934 1222 1384 1561 1666 1678 1846 2307
Size: 901 288 162 177 105 12 168 461
Cuts at: 2307 2992 3985 4176 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 901 685 461 288 191 177
168 162 105 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 1252 1252
Size: 5264
SfoI GGC'GCC
Cuts at: 1063 2150 2696 2944 1063
Size: 1087 546 248 3383
Fragments arranged by size:
3383 1087 546 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5264
SmaI CCC'GGG
Cuts at: 2286 2286
Size: 5264
SmlI C'TyrA_G
Cuts at: 2419 3334 3826 4088 2419
Size: 915 492 262 3595
Fragments arranged by size:
3595 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5264
SphI G_CATG'C
Cuts at: 2317 2817 2317
Size: 500 4764
StyI C'CwwG_G
Cuts at: 1886 1940 2213 2398 2752 1886
Size: 54 273 185 354 4398
Fragments arranged by size:
4398 354 273 185 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1824 2847 2980 3439 3648 1824
Size: 1023 133 459 209 3440
Fragments arranged by size:
3440 1023 459 209 133
TatI w'GTAC_w
Cuts at: 350 1787 4779 350
Size: 1437 2992 835
Fragments arranged by size:
2992 1437 835
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4781 4813 4781
Size: 32 5232
Tth111I GACn'n_nGTC
Cuts at: 3061 3061
Size: 5264
XbaI T'CTAG_A
Cuts at: 2295 2295
Size: 5264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 296 2759 296
Size: 2463 2801
XmaI C'CCGG_G
Cuts at: 2284 2284
Size: 5264
XmnI GAAnn'nnTTC
Cuts at: 530 530
Size: 5264
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PmeI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BcgI BfrBI BmgBI
BmtI BsiWI BsrGI BstEII BstZ17I ClaI CspCI DraIII
EcoRV FspAI HpaI NdeI NheI NotI NruI NsiI
PacI PasI PfoI PmlI PshAI PsiI PspXI PsrI
SanDI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI