Restriction map for
pF1KB5714
(Circular) (Six-base) MAPSORT of: pF1KB5714.seq Check: 9292 from: 1 to: 5276
pF1KB5714 5276 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1745 1745
Size: 5276
AccI GT'mk_AC
Cuts at: 422 2314 422
Size: 1892 3384
Acc65I G'GTAC_C
Cuts at: 2292 2292
Size: 5276
AclI AA'CG_TT
Cuts at: 5116 5116
Size: 5276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3101 3533 4280 4548 3101
Size: 432 747 268 3829
Fragments arranged by size:
3829 747 432 268
AflIII A'CryG_T
Cuts at: 2628 3732 4709 2628
Size: 1104 977 3195
Fragments arranged by size:
3195 1104 977
AgeI A'CCGG_T
Cuts at: 3664 3664
Size: 5276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 281 281
Size: 5276
AlwNI CAG_nnn'CTG
Cuts at: 1116 1149 2581 4148 1116
Size: 33 1432 1567 2244
Fragments arranged by size:
2244 1567 1432 33
ApaLI G'TGCA_C
Cuts at: 4046 4046
Size: 5276
ApoI r'AATT_y
Cuts at: 1370 2084 2280 4629 4885 1370
Size: 714 196 2349 256 1761
Fragments arranged by size:
2349 1761 714 256 196
AseI AT'TA_AT
Cuts at: 20 20
Size: 5276
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5276
AvaI C'yCGr_G
Cuts at: 2296 2296
Size: 5276
BamHI G'GATC_C
Cuts at: 2301 2301
Size: 5276
BanI G'GyrC_C
Cuts at: 2292 2706 2954 2989 2292
Size: 414 248 35 4579
Fragments arranged by size:
4579 414 248 35
BanII G_rGCy'C
Cuts at: 289 2290 3320 289
Size: 2001 1030 2245
Fragments arranged by size:
2245 2001 1030
BbeI G_GCGC'C
Cuts at: 2710 2958 2710
Size: 248 5028
BbsI GAAGACnn'nnnn_
Cuts at: 270 1679 1847 270
Size: 1409 168 3699
Fragments arranged by size:
3699 1409 168
BbvCI CC'TCA_GC
Cuts at: 1991 1991
Size: 5276
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 5242
BciVI GTATCCnnnnn_n'
Cuts at: 3174 3935 5276 3174
Size: 761 1341 3174
Fragments arranged by size:
3174 1341 761
BclI T'GATC_A
Cuts at: 1953 2189 2796 1953
Size: 236 607 4433
Fragments arranged by size:
4433 607 236
BglII A'GATC_T
Cuts at: 2791 4828 2791
Size: 2037 3239
BlpI GC'TnA_GC
Cuts at: 2388 2388
Size: 5276
Bme1580I G_kGCm'C
Cuts at: 1629 2901 2994 4050 1629
Size: 1272 93 1056 2855
Fragments arranged by size:
2855 1272 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 944 1503 2488 2569 2904 5048 944
Size: 559 985 81 335 2144 1172
Fragments arranged by size:
2144 1172 985 559 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 5276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 834 1404 605
Size: 229 570 4477
Fragments arranged by size:
4477 570 229
Bpu10I CC'TnA_GC
Cuts at: 362 1991 362
Size: 1629 3647
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2452 3331 3823 4121 2452
Size: 879 492 298 3607
Fragments arranged by size:
3607 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1282 4980 1282
Size: 3698 1578
BsaAI yAC'GTr
Cuts at: 1392 2629 1392
Size: 1237 4039
BsaBI GATnn'nnATC
Cuts at: 6 1223 1913 2790 4827 6
Size: 1217 690 877 2037 455
Fragments arranged by size:
2037 1217 877 690 455
BsaHI Gr'CG_yC
Cuts at: 2707 2955 3657 5153 2707
Size: 248 702 1496 2830
Fragments arranged by size:
2830 1496 702 248
BsaWI w'CCGG_w
Cuts at: 998 2465 2986 3664 3938 4085 4496 998
Size: 1467 521 678 274 147 411 1778
Fragments arranged by size:
1778 1467 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 578 608 1402 1432 578
Size: 30 794 30 4422
Fragments arranged by size:
4422 794 30 30
BseYI C'CCAG_C
Cuts at: 480 1239 1402 2701 3459 4036 4532 480
Size: 759 163 1299 758 577 496 1224
Fragments arranged by size:
1299 1224 759 758 577 496 163
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1124 1456 1124
Size: 332 4944
BsiEI CG_ry'CG
Cuts at: 104 206 2818 2864 4072 104
Size: 102 2612 46 1208 1308
Fragments arranged by size:
2612 1308 1208 102 46
BsiHKAI G_wGCw'C
Cuts at: 537 2290 3068 3258 4050 537
Size: 1753 778 190 792 1763
Fragments arranged by size:
1763 1753 792 778 190
BsmI GAATG_Cn'
Cuts at: 845 1406 1483 1695 845
Size: 561 77 212 4426
Fragments arranged by size:
4426 561 212 77
Bsp1286I G_dGCh'C
Cuts at: 289 537 1629 2290 2901 2994 3068 3258 3320
Size: 248 1092 661 611 93 74 190 62
Cuts at: 3320 4050 289
Size: 730 1515
Fragments arranged by size:
1515 1092 730 661 611 248 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4496 4496
Size: 5276
BspHI T'CATG_A
Cuts at: 552 4452 552
Size: 3900 1376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1067 1745 2143 2326 2540 2842 3223 1067
Size: 678 398 183 214 302 381 3120
Fragments arranged by size:
3120 678 398 381 302 214 183
BsrBI CCG'CTC
Cuts at: 50 3568 3622 5106 5271 50
Size: 3518 54 1484 165 55
Fragments arranged by size:
3518 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1613 3188 1613
Size: 1575 3701
BsrFI r'CCGG_y
Cuts at: 976 3274 3455 3664 976
Size: 2298 181 209 2588
Fragments arranged by size:
2588 2298 209 181
BssSI C'ACGA_G
Cuts at: 3547 3905 3547
Size: 358 4918
BstAPI GCAn_nnn'nTGC
Cuts at: 2653 2653
Size: 5276
BstBI TT'CG_AA
Cuts at: 2214 3637 2214
Size: 1423 3853
BstYI r'GATC_y
Cuts at: 1 652 1467 2301 2768 2791 3126 3372 4384
Size: 651 815 834 467 23 335 246 1012
Cuts at: 4384 4470 4482 4828 1
Size: 86 12 346 449
Fragments arranged by size:
1012 834 815 651 467 449 346 335
246 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3226 3407 3570 3226
Size: 181 163 4932
Fragments arranged by size:
4932 181 163
BtsI GCAGTG_nn'
Cuts at: 2192 2519 2192
Size: 327 4949
DraI TTT'AAA
Cuts at: 2275 2275
Size: 5276
DrdI GACnn_nn'nnGTC
Cuts at: 2982 3840 2982
Size: 858 4418
EaeI y'GGCC_r
Cuts at: 164 203 1008 1196 1731 2861 3035 3426 3453
Size: 39 805 188 535 1130 174 391 27
Cuts at: 3453 4798 164
Size: 1345 642
Fragments arranged by size:
1345 1130 805 642 535 391 188 174
39 27
EagI C'GGCC_G
Cuts at: 203 2861 203
Size: 2658 2618
EarI CTCTTCn'nnn_
Cuts at: 314 2023 3299 3509 314
Size: 1709 1276 210 2081
Fragments arranged by size:
2081 1709 1276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3484 3794 3940 5086 3484
Size: 310 146 1146 3674
Fragments arranged by size:
3674 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2288 2288
Size: 5276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 605 834 1404 3101 3533 4280 4548 605
Size: 229 570 1697 432 747 268 1333
Fragments arranged by size:
1697 1333 747 570 432 268 229
EcoO109I rG'GnC_Cy
Cuts at: 1113 2415 1113
Size: 1302 3974
EcoRI G'AATT_C
Cuts at: 2280 2280
Size: 5276
EcoRV GAT'ATC
Cuts at: 669 1806 669
Size: 1137 4139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 661 3295 661
Size: 2634 2642
FspI TGC'GCA
Cuts at: 3057 3057
Size: 5276
HaeII r_GCGC'y
Cuts at: 177 2710 2958 3980 4959 177
Size: 2533 248 1022 979 494
Fragments arranged by size:
2533 1022 979 494 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 787 819 3118 3148 3150 3180 787
Size: 32 2299 30 2 30 2883
Fragments arranged by size:
2883 2299 32 30 30 2
HincII GTy'rAC
Cuts at: 1605 2315 1605
Size: 710 4566
HpaI GTT'AAC
Cuts at: 1605 1605
Size: 5276
KasI G'GCGC_C
Cuts at: 2706 2954 2706
Size: 248 5028
KpnI G_GTAC'C
Cuts at: 2296 2296
Size: 5276
MfeI C'AATT_G
Cuts at: 473 528 971 473
Size: 55 443 4778
Fragments arranged by size:
4778 443 55
MluI A'CGCG_T
Cuts at: 4709 4709
Size: 5276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 630 765 1965 2148 3947 4131 630
Size: 135 1200 183 1799 184 1775
Fragments arranged by size:
1799 1775 1200 184 183 135
MscI TGG'CCA
Cuts at: 166 1010 1198 3037 166
Size: 844 188 1839 2405
Fragments arranged by size:
2405 1839 844 188
MslI CAynn'nnrTG
Cuts at: 263 2251 3265 3392 263
Size: 1988 1014 127 2147
Fragments arranged by size:
2147 1988 1014 127
MspA1I CmG'CkG
Cuts at: 335 2387 2701 3061 4074 4319 335
Size: 2052 314 360 1013 245 1292
Fragments arranged by size:
2052 1292 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3457 3457
Size: 5276
NarI GG'CG_CC
Cuts at: 2707 2955 2707
Size: 248 5028
NgoMIV G'CCGG_C
Cuts at: 3455 3455
Size: 5276
NotI GC'GGCC_GC
Cuts at: 203 203
Size: 5276
NspI r_CATG'y
Cuts at: 262 2329 2829 3736 262
Size: 2067 500 907 1802
Fragments arranged by size:
2067 1802 907 500
PciI A'CATG_T
Cuts at: 3732 3732
Size: 5276
PflMI CCAn_nnn'nTGG
Cuts at: 1818 1818
Size: 5276
PmeI GTTT'AAAC
Cuts at: 2275 2275
Size: 5276
PmlI CAC'GTG
Cuts at: 1392 1392
Size: 5276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 4415
Size: 32 5244
PpuMI rG'GwC_Cy
Cuts at: 1113 1113
Size: 5276
PstI C_TGCA'G
Cuts at: 348 2323 3008 348
Size: 1975 685 2616
Fragments arranged by size:
2616 1975 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5276
PvuII CAG'CTG
Cuts at: 335 2701 3061 335
Size: 2366 360 2550
Fragments arranged by size:
2550 2366 360
RsrII CG'GwC_CG
Cuts at: 3471 3471
Size: 5276
SacI G_AGCT'C
Cuts at: 2290 2290
Size: 5276
SalI G'TCGA_C
Cuts at: 2313 2313
Size: 5276
SapI GCTCTTCn'nnn_
Cuts at: 3299 3509 3299
Size: 210 5066
SbfI CC_TGCA'GG
Cuts at: 2323 2323
Size: 5276
ScaI AGT'ACT
Cuts at: 4793 4793
Size: 5276
SexAI A'CCwGG_T
Cuts at: 2183 2183
Size: 5276
SfcI C'TryA_G
Cuts at: 33 344 444 2319 3004 3997 4188 33
Size: 311 100 1875 685 993 191 1121
Fragments arranged by size:
1875 1121 993 685 311 191 100
SfoI GGC'GCC
Cuts at: 2708 2956 2708
Size: 248 5028
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5276
SmaI CCC'GGG
Cuts at: 2298 2298
Size: 5276
SmlI C'TyrA_G
Cuts at: 2431 3346 3838 4100 2431
Size: 915 492 262 3607
Fragments arranged by size:
3607 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5276
SphI G_CATG'C
Cuts at: 2329 2829 2329
Size: 500 4776
StyI C'CwwG_G
Cuts at: 602 1184 2410 2764 602
Size: 582 1226 354 3114
Fragments arranged by size:
3114 1226 582 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 226 944 1522 1639 2859 2992 3451 3660 226
Size: 718 578 117 1220 133 459 209 1842
Fragments arranged by size:
1842 1220 718 578 459 209 133 117
TatI w'GTAC_w
Cuts at: 4791 4791
Size: 5276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1402 1434 4793 4825 1402
Size: 32 3359 32 1853
Fragments arranged by size:
3359 1853 32 32
Tth111I GACn'n_nGTC
Cuts at: 3073 3073
Size: 5276
XbaI T'CTAG_A
Cuts at: 2307 2307
Size: 5276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1191 2771 1191
Size: 1580 3696
XmaI C'CCGG_G
Cuts at: 2296 2296
Size: 5276
XmnI GAAnn'nnTTC
Cuts at: 1483 1844 1483
Size: 361 4915
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NotI NspI PciI PflMI PmeI
PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
AvrII BaeI BfrBI BglI BmgBI BmtI BseRI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI CspCI DraIII EcoNI FseI FspAI HindIII NcoI
NdeI NheI NruI NsiI PacI PasI PfoI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI