Restriction map for
pF1KB5715
(Circular) (Six-base) MAPSORT of: pF1KB5715.seq Check: 560 from: 1 to: 5474
pF1KB5715 5474 bp
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1199 1236 2245 1199
Size: 37 1009 4428
Fragments arranged by size:
4428 1009 37
AccI GT'mk_AC
Cuts at: 494 2512 494
Size: 2018 3456
Acc65I G'GTAC_C
Cuts at: 2490 2490
Size: 5474
AclI AA'CG_TT
Cuts at: 5314 5314
Size: 5474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1491 1857 3299 3731 4478 4746 653
Size: 838 366 1442 432 747 268 1381
Fragments arranged by size:
1442 1381 838 747 432 366 268
AflIII A'CryG_T
Cuts at: 335 2826 3930 4907 335
Size: 2491 1104 977 902
Fragments arranged by size:
2491 1104 977 902
AgeI A'CCGG_T
Cuts at: 3862 3862
Size: 5474
AleI CACnn'nnGTG
Cuts at: 862 2029 862
Size: 1167 4307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 442 442
Size: 5474
AlwNI CAG_nnn'CTG
Cuts at: 480 781 1866 2403 2779 4346 480
Size: 301 1085 537 376 1567 1608
Fragments arranged by size:
1608 1567 1085 537 376 301
ApaI G_GGCC'C
Cuts at: 1904 1965 1904
Size: 61 5413
ApaLI G'TGCA_C
Cuts at: 2360 4244 2360
Size: 1884 3590
ApoI r'AATT_y
Cuts at: 2478 4827 5083 2478
Size: 2349 256 2869
Fragments arranged by size:
2869 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5474
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
AvaI C'yCGr_G
Cuts at: 183 201 557 1167 1408 1644 2494 183
Size: 18 356 610 241 236 850 3163
Fragments arranged by size:
3163 850 610 356 241 236 18
BamHI G'GATC_C
Cuts at: 2343 2499 2343
Size: 156 5318
BanI G'GyrC_C
Cuts at: 970 1003 1147 2163 2257 2490 2904 3152 3187
Size: 33 144 1016 94 233 414 248 35
Cuts at: 3187 970
Size: 3257
Fragments arranged by size:
3257 1016 414 248 233 144 94 35
33
BanII G_rGCy'C
Cuts at: 143 349 1025 1844 1904 1937 1965 2330 2385
Size: 206 676 819 60 33 28 365 55
Cuts at: 2385 2488 3518 143
Size: 103 1030 2099
Fragments arranged by size:
2099 1030 819 676 365 206 103 60
55 33 28
BbeI G_GCGC'C
Cuts at: 2167 2908 3156 2167
Size: 741 248 4485
Fragments arranged by size:
4485 741 248
BbvCI CC'TCA_GC
Cuts at: 1938 2222 1938
Size: 284 5190
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 633 667 633
Size: 34 5440
BciVI GTATCCnnnnn_n'
Cuts at: 3372 4133 5474 3372
Size: 761 1341 3372
Fragments arranged by size:
3372 1341 761
BclI T'GATC_A
Cuts at: 2994 2994
Size: 5474
BglI GCCn_nnn'nGGC
Cuts at: 299 820 1084 2374 2399 299
Size: 521 264 1290 25 3374
Fragments arranged by size:
3374 1290 521 264 25
BglII A'GATC_T
Cuts at: 2087 2989 5026 2087
Size: 902 2037 2535
Fragments arranged by size:
2535 2037 902
BlpI GC'TnA_GC
Cuts at: 289 782 2586 289
Size: 493 1804 3177
Fragments arranged by size:
3177 1804 493
Bme1580I G_kGCm'C
Cuts at: 695 975 1150 1904 1965 2048 2158 2364 3099
Size: 280 175 754 61 83 110 206 735
Cuts at: 3099 3192 4248 695
Size: 93 1056 1921
Fragments arranged by size:
1921 1056 754 735 280 206 175 110
93 83 61
BmgBI CAC'GTC
Cuts at: 735 735
Size: 5474
BmrI ACTGGGnnnn_n'
Cuts at: 951 1775 2195 2324 2351 2686 2767 3102 5246
Size: 824 420 129 27 335 81 335 2144
Cuts at: 5246 951
Size: 1179
Fragments arranged by size:
2144 1179 824 420 335 335 129 81
27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1044 3841 1044
Size: 2797 2677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1761 2024 1761
Size: 263 5211
Bpu10I CC'TnA_GC
Cuts at: 1938 2174 2222 1938
Size: 236 48 5190
Fragments arranged by size:
5190 236 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2650 3529 4021 4319 2650
Size: 879 492 298 3805
Fragments arranged by size:
3805 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1441 2310 5178 1441
Size: 869 2868 1737
Fragments arranged by size:
2868 1737 869
BsaAI yAC'GTr
Cuts at: 2827 2827
Size: 5474
BsaBI GATnn'nnATC
Cuts at: 6 2988 5025 6
Size: 2982 2037 455
Fragments arranged by size:
2982 2037 455
BsaHI Gr'CG_yC
Cuts at: 2164 2905 3153 3855 5351 2164
Size: 741 248 702 1496 2287
Fragments arranged by size:
2287 1496 741 702 248
BsaWI w'CCGG_w
Cuts at: 443 2663 3184 3862 4136 4283 4694 443
Size: 2220 521 678 274 147 411 1223
Fragments arranged by size:
2220 1223 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 261 291 382 412 261
Size: 30 91 30 5323
Fragments arranged by size:
5323 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 267 270 1385 1941 2009 2145 267
Size: 3 1115 556 68 136 3596
Fragments arranged by size:
3596 1115 556 136 68 3
BseYI C'CCAG_C
Cuts at: 123 174 461 532 1964 2269 2899 3657 4234
Size: 51 287 71 1432 305 630 758 577
Cuts at: 4234 4730 123
Size: 496 867
Fragments arranged by size:
1432 867 758 630 577 496 305 287
71 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 597 624 673 822 1016 1776 597
Size: 27 49 149 194 760 4295
Fragments arranged by size:
4295 760 194 149 49 27
BsiEI CG_ry'CG
Cuts at: 104 218 303 3016 3062 4270 104
Size: 114 85 2713 46 1208 1308
Fragments arranged by size:
2713 1308 1208 114 85 46
BsiHKAI G_wGCw'C
Cuts at: 143 431 788 1343 1499 1708 1844 2364 2488
Size: 288 357 555 156 209 136 520 124
Cuts at: 2488 3266 3456 4248 143
Size: 778 190 792 1369
Fragments arranged by size:
1369 792 778 555 520 357 288 209
190 156 136 124
BsmI GAATG_Cn'
Cuts at: 453 453
Size: 5474
Bsp1286I G_dGCh'C
Cuts at: 143 349 431 695 788 975 1025 1150 1343
Size: 206 82 264 93 187 50 125 193
Cuts at: 1343 1499 1708 1844 1904 1937 1965 2048 2158
Size: 156 209 136 60 33 28 83 110
Cuts at: 2158 2330 2364 2385 2488 3099 3192 3266 3456
Size: 172 34 21 103 611 93 74 190
Cuts at: 3456 3518 4248 143
Size: 62 730 1369
Fragments arranged by size:
1369 730 611 264 209 206 193 190
187 172 156 136 125 110 103 93
93 83 82 74 62 60 50 34
33 28 21
BspEI T'CCGG_A
Cuts at: 443 4694 443
Size: 4251 1223
BspHI T'CATG_A
Cuts at: 1376 4650 1376
Size: 3274 2200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1199 1236 1748 2245 2524 2738 3040 3421 1199
Size: 37 512 497 279 214 302 381 3252
Fragments arranged by size:
3252 512 497 381 302 279 214 37
BsrBI CCG'CTC
Cuts at: 50 147 206 588 768 3766 3820 5304 5469
Size: 97 59 382 180 2998 54 1484 165
Cuts at: 5469 50
Size: 55
Fragments arranged by size:
2998 1484 382 180 165 97 59 55
54
BsrDI GCAATG_nn'
Cuts at: 3386 3386
Size: 5474
BsrFI r'CCGG_y
Cuts at: 2364 3472 3653 3862 2364
Size: 1108 181 209 3976
Fragments arranged by size:
3976 1108 209 181
BssSI C'ACGA_G
Cuts at: 424 1837 3745 4103 424
Size: 1413 1908 358 1795
Fragments arranged by size:
1908 1795 1413 358
BstAPI GCAn_nnn'nTGC
Cuts at: 755 1234 2851 755
Size: 479 1617 3378
Fragments arranged by size:
3378 1617 479
BstBI TT'CG_AA
Cuts at: 3835 3835
Size: 5474
BstXI CCAn_nnnn'nTGG
Cuts at: 381 864 1983 2433 381
Size: 483 1119 450 3422
Fragments arranged by size:
3422 1119 483 450
BstYI r'GATC_y
Cuts at: 1 2087 2343 2499 2966 2989 3324 3570 4582
Size: 2086 256 156 467 23 335 246 1012
Cuts at: 4582 4668 4680 5026 1
Size: 86 12 346 449
Fragments arranged by size:
2086 1012 467 449 346 335 256 246
156 86 23 12
BstZ17I GTA'TAC
Cuts at: 495 495
Size: 5474
Bsu36I CC'TnA_GG
Cuts at: 2339 2339
Size: 5474
BtgI C'CryG_G
Cuts at: 107 1904 2372 107
Size: 1797 468 3209
Fragments arranged by size:
3209 1797 468
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 792 1680 3424 3605 3768 792
Size: 888 1744 181 163 2498
Fragments arranged by size:
2498 1744 888 181 163
BtsI GCAGTG_nn'
Cuts at: 294 376 473 2717 294
Size: 82 97 2244 3051
Fragments arranged by size:
3051 2244 97 82
DraI TTT'AAA
Cuts at: 2473 2473
Size: 5474
DraIII CAC_nnn'GTG
Cuts at: 1887 1887
Size: 5474
DrdI GACnn_nn'nnGTC
Cuts at: 3180 4038 3180
Size: 858 4616
EaeI y'GGCC_r
Cuts at: 110 300 383 854 2388 3059 3233 3624 3651
Size: 190 83 471 1534 671 174 391 27
Cuts at: 3651 4996 110
Size: 1345 588
Fragments arranged by size:
1534 1345 671 588 471 391 190 174
83 27
EagI C'GGCC_G
Cuts at: 300 3059 300
Size: 2759 2715
EarI CTCTTCn'nnn_
Cuts at: 775 850 913 1795 2122 2131 3497 3707 775
Size: 75 63 882 327 9 1366 210 2542
Fragments arranged by size:
2542 1366 882 327 210 75 63 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 269 891 3682 3992 4138 5284 269
Size: 622 2791 310 146 1146 459
Fragments arranged by size:
2791 1146 622 459 310 146
EcoICRI GAG'CTC
Cuts at: 141 1842 2486 141
Size: 1701 644 3129
Fragments arranged by size:
3129 1701 644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 653 1491 1761 1857 2024 3299 3731 4478 4746
Size: 838 270 96 167 1275 432 747 268
Cuts at: 4746 653
Size: 1381
Fragments arranged by size:
1381 1275 838 747 432 270 268 167
96
EcoNI CCTnn'n_nnAGG
Cuts at: 547 1755 547
Size: 1208 4266
EcoO109I rG'GnC_Cy
Cuts at: 180 562 2400 2407 2613 180
Size: 382 1838 7 206 3041
Fragments arranged by size:
3041 1838 382 206 7
EcoRI G'AATT_C
Cuts at: 2478 2478
Size: 5474
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 5474
FspI TGC'GCA
Cuts at: 3255 3255
Size: 5474
HaeII r_GCGC'y
Cuts at: 2112 2167 2908 3156 4178 5157 2112
Size: 55 741 248 1022 979 2429
Fragments arranged by size:
2429 1022 979 741 248 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 261 293 382 414 971 1003 1027 1059 1243
Size: 32 89 32 557 32 24 32 184
Cuts at: 1243 1275 1623 1655 1936 1968 1971 2003 2443
Size: 32 348 32 281 32 3 32 440
Cuts at: 2443 2475 3316 3346 3348 3378 261
Size: 32 841 30 2 30 2357
Fragments arranged by size:
2357 841 557 440 348 281 184 89
32 32 32 32 32 32 32 32
32 30 30 24 3 2
HincII GTy'rAC
Cuts at: 1763 2513 1763
Size: 750 4724
KasI G'GCGC_C
Cuts at: 2163 2904 3152 2163
Size: 741 248 4485
Fragments arranged by size:
4485 741 248
KpnI G_GTAC'C
Cuts at: 2494 2494
Size: 5474
MluI A'CGCG_T
Cuts at: 4907 4907
Size: 5474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2333 4145 4329 2333
Size: 1812 184 3478
Fragments arranged by size:
3478 1812 184
MscI TGG'CCA
Cuts at: 856 3235 856
Size: 2379 3095
MslI CAynn'nnrTG
Cuts at: 862 1699 1978 2029 3463 3590 862
Size: 837 279 51 1434 127 2746
Fragments arranged by size:
2746 1434 837 279 127 51
MspA1I CmG'CkG
Cuts at: 115 288 324 458 1511 1942 2160 2226 2585
Size: 173 36 134 1053 431 218 66 359
Cuts at: 2585 2899 3259 4272 4517 115
Size: 314 360 1013 245 1072
Fragments arranged by size:
1072 1053 1013 431 360 359 314 245
218 173 134 66 36
NaeI GCC'GGC
Cuts at: 3655 3655
Size: 5474
NarI GG'CG_CC
Cuts at: 2164 2905 3153 2164
Size: 741 248 4485
Fragments arranged by size:
4485 741 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5474
NgoMIV G'CCGG_C
Cuts at: 3653 3653
Size: 5474
NspI r_CATG'y
Cuts at: 501 798 1881 1972 2527 3027 3934 501
Size: 297 1083 91 555 500 907 2041
Fragments arranged by size:
2041 1083 907 555 500 297 91
PasI CC'CwG_GG
Cuts at: 1143 1143
Size: 5474
PciI A'CATG_T
Cuts at: 3930 3930
Size: 5474
PflMI CCAn_nnn'nTGG
Cuts at: 722 963 2069 722
Size: 241 1106 4127
Fragments arranged by size:
4127 1106 241
PfoI T'CCnGG_A
Cuts at: 1559 1559
Size: 5474
PmeI GTTT'AAAC
Cuts at: 2473 2473
Size: 5474
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4613 4645 4613
Size: 32 5442
PpuMI rG'GwC_Cy
Cuts at: 180 180
Size: 5474
PshAI GACnn'nnGTC
Cuts at: 2302 2302
Size: 5474
PspOMI G'GGCC_C
Cuts at: 1900 1961 1900
Size: 61 5413
PspXI vC'TCGA_Gb
Cuts at: 201 201
Size: 5474
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1285 1317 1285
Size: 32 5442
PstI C_TGCA'G
Cuts at: 1850 2105 2256 2521 3206 1850
Size: 255 151 265 685 4118
Fragments arranged by size:
4118 685 265 255 151
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5474
PvuII CAG'CTG
Cuts at: 1511 1942 2226 2899 3259 1511
Size: 431 284 673 360 3726
Fragments arranged by size:
3726 673 431 360 284
RsrII CG'GwC_CG
Cuts at: 3669 3669
Size: 5474
SacI G_AGCT'C
Cuts at: 143 1844 2488 143
Size: 1701 644 3129
Fragments arranged by size:
3129 1701 644
SalI G'TCGA_C
Cuts at: 2511 2511
Size: 5474
SanDI GG'GwC_CC
Cuts at: 180 180
Size: 5474
SapI GCTCTTCn'nnn_
Cuts at: 775 850 1795 3497 3707 775
Size: 75 945 1702 210 2542
Fragments arranged by size:
2542 1702 945 210 75
SbfI CC_TGCA'GG
Cuts at: 2521 2521
Size: 5474
ScaI AGT'ACT
Cuts at: 4991 4991
Size: 5474
SexAI A'CCwGG_T
Cuts at: 1198 1750 1198
Size: 552 4922
SfcI C'TryA_G
Cuts at: 33 1846 2101 2252 2517 3202 4195 4386 33
Size: 1813 255 151 265 685 993 191 1121
Fragments arranged by size:
1813 1121 993 685 265 255 191 151
SfiI GGCCn_nnn'nGGCC
Cuts at: 820 820
Size: 5474
SfoI GGC'GCC
Cuts at: 2165 2906 3154 2165
Size: 741 248 4485
Fragments arranged by size:
4485 741 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5474
SmaI CCC'GGG
Cuts at: 185 1410 2496 185
Size: 1225 1086 3163
Fragments arranged by size:
3163 1225 1086
SmlI C'TyrA_G
Cuts at: 201 2629 3544 4036 4298 201
Size: 2428 915 492 262 1377
Fragments arranged by size:
2428 1377 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5474
SphI G_CATG'C
Cuts at: 1881 2527 3027 1881
Size: 646 500 4328
Fragments arranged by size:
4328 646 500
StuI AGG'CCT
Cuts at: 1822 1822
Size: 5474
StyI C'CwwG_G
Cuts at: 107 695 1721 1817 2608 2962 107
Size: 588 1026 96 791 354 2619
Fragments arranged by size:
2619 1026 791 588 354 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3057 3190 3649 3858 3057
Size: 133 459 209 4673
Fragments arranged by size:
4673 459 209 133
TatI w'GTAC_w
Cuts at: 1070 4989 1070
Size: 3919 1555
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1873 1905 4991 5023 1873
Size: 32 3086 32 2324
Fragments arranged by size:
3086 2324 32 32
Tth111I GACn'n_nGTC
Cuts at: 1041 3271 1041
Size: 2230 3244
XbaI T'CTAG_A
Cuts at: 2505 2505
Size: 5474
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 964 1458 2070 2969 964
Size: 494 612 899 3469
Fragments arranged by size:
3469 899 612 494
XhoI C'TCGA_G
Cuts at: 201 201
Size: 5474
XmaI C'CCGG_G
Cuts at: 183 1408 2494 183
Size: 1225 1086 3163
Fragments arranged by size:
3163 1225 1086
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PsiI
SacII SgrAI SnaBI SrfI SspI SwaI XmnI ZraI