Restriction map for
pF1KB5716
(Circular) (Six-base) MAPSORT of: pF1KB5716.seq Check: 2531 from: 1 to: 5276
pF1KB5716 5276 bp
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 531 531
Size: 5276
AccI GT'mk_AC
Cuts at: 1956 2314 1956
Size: 358 4918
Acc65I G'GTAC_C
Cuts at: 2292 2292
Size: 5276
AclI AA'CG_TT
Cuts at: 5116 5116
Size: 5276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 385 664 1120 1507 1532 3101 3533 4280 4548
Size: 279 456 387 25 1569 432 747 268
Cuts at: 4548 385
Size: 1113
Fragments arranged by size:
1569 1113 747 456 432 387 279 268
25
AfeI AGC'GCT
Cuts at: 133 133
Size: 5276
AflIII A'CryG_T
Cuts at: 2169 2210 2628 3732 4709 2169
Size: 41 418 1104 977 2736
Fragments arranged by size:
2736 1104 977 418 41
AgeI A'CCGG_T
Cuts at: 1090 3664 1090
Size: 2574 2702
AhdI GACnn_n'nnGTC
Cuts at: 1613 1613
Size: 5276
AleI CACnn'nnGTG
Cuts at: 1250 1250
Size: 5276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1048 1268 1048
Size: 220 5056
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 622 654 622
Size: 32 5244
AlwNI CAG_nnn'CTG
Cuts at: 1166 1242 2581 4148 1166
Size: 76 1339 1567 2294
Fragments arranged by size:
2294 1567 1339 76
ApaLI G'TGCA_C
Cuts at: 823 4046 823
Size: 3223 2053
ApoI r'AATT_y
Cuts at: 2280 4629 4885 2280
Size: 2349 256 2671
Fragments arranged by size:
2671 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5276
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5276
AvaI C'yCGr_G
Cuts at: 1603 1867 2296 1603
Size: 264 429 4583
Fragments arranged by size:
4583 429 264
BamHI G'GATC_C
Cuts at: 2301 2301
Size: 5276
BanI G'GyrC_C
Cuts at: 284 844 1087 1293 2292 2706 2954 2989 284
Size: 560 243 206 999 414 248 35 2571
Fragments arranged by size:
2571 999 560 414 248 243 206 35
BanII G_rGCy'C
Cuts at: 2087 2180 2290 3320 2087
Size: 93 110 1030 4043
Fragments arranged by size:
4043 1030 110 93
BbeI G_GCGC'C
Cuts at: 288 2710 2958 288
Size: 2422 248 2606
Fragments arranged by size:
2606 2422 248
BbsI GAAGACnn'nnnn_
Cuts at: 1093 1946 1093
Size: 853 4423
BbvCI CC'TCA_GC
Cuts at: 274 769 1077 274
Size: 495 308 4473
Fragments arranged by size:
4473 495 308
BciVI GTATCCnnnnn_n'
Cuts at: 1517 3174 3935 5276 1517
Size: 1657 761 1341 1517
Fragments arranged by size:
1657 1517 1341 761
BclI T'GATC_A
Cuts at: 2796 2796
Size: 5276
BglI GCCn_nnn'nGGC
Cuts at: 1157 1157
Size: 5276
BglII A'GATC_T
Cuts at: 1673 2791 4828 1673
Size: 1118 2037 2121
Fragments arranged by size:
2121 2037 1118
BlpI GC'TnA_GC
Cuts at: 656 784 2388 656
Size: 128 1604 3544
Fragments arranged by size:
3544 1604 128
Bme1580I G_kGCm'C
Cuts at: 827 947 1090 2901 2994 4050 827
Size: 120 143 1811 93 1056 2053
Fragments arranged by size:
2053 1811 1056 143 120 93
BmrI ACTGGGnnnn_n'
Cuts at: 489 1592 2488 2569 2904 5048 489
Size: 1103 896 81 335 2144 717
Fragments arranged by size:
2144 1103 896 717 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3643 3643
Size: 5276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 413 1429 1561 1797 2011 2164 323
Size: 90 1016 132 236 214 153 3435
Fragments arranged by size:
3435 1016 236 214 153 132 90
Bpu10I CC'TnA_GC
Cuts at: 274 769 1077 1921 1934 274
Size: 495 308 844 13 3616
Fragments arranged by size:
3616 844 495 308 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 847 2452 3331 3823 4121 847
Size: 1605 879 492 298 2002
Fragments arranged by size:
2002 1605 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 570 694 1523 4980 570
Size: 124 829 3457 866
Fragments arranged by size:
3457 866 829 124
BsaAI yAC'GTr
Cuts at: 828 2629 828
Size: 1801 3475
BsaBI GATnn'nnATC
Cuts at: 6 2068 2790 4827 6
Size: 2062 722 2037 455
Fragments arranged by size:
2062 2037 722 455
BsaHI Gr'CG_yC
Cuts at: 285 1098 2707 2955 3657 5153 285
Size: 813 1609 248 702 1496 408
Fragments arranged by size:
1609 1496 813 702 408 248
BsaWI w'CCGG_w
Cuts at: 1090 2465 2986 3664 3938 4085 4496 1090
Size: 1375 521 678 274 147 411 1870
Fragments arranged by size:
1870 1375 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 658 688 1256 1286 658
Size: 30 568 30 4648
Fragments arranged by size:
4648 568 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 268 312 496 574 1141 2115 268
Size: 44 184 78 567 974 3429
Fragments arranged by size:
3429 974 567 184 78 44
BseYI C'CCAG_C
Cuts at: 1323 1335 1642 2701 3459 4036 4532 1323
Size: 12 307 1059 758 577 496 2067
Fragments arranged by size:
2067 1059 758 577 496 307 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 368 491 1228 1738 1774 368
Size: 123 737 510 36 3870
Fragments arranged by size:
3870 737 510 123 36
BsiEI CG_ry'CG
Cuts at: 104 972 2818 2864 4072 104
Size: 868 1846 46 1208 1308
Fragments arranged by size:
1846 1308 1208 868 46
BsiHKAI G_wGCw'C
Cuts at: 260 827 1257 2290 3068 3258 4050 260
Size: 567 430 1033 778 190 792 1486
Fragments arranged by size:
1486 1033 792 778 567 430 190
Bsp1286I G_dGCh'C
Cuts at: 260 827 947 1090 1257 2087 2180 2290 2901
Size: 567 120 143 167 830 93 110 611
Cuts at: 2901 2994 3068 3258 3320 4050 260
Size: 93 74 190 62 730 1486
Fragments arranged by size:
1486 830 730 611 567 190 167 143
120 110 93 93 74 62
BspEI T'CCGG_A
Cuts at: 4496 4496
Size: 5276
BspHI T'CATG_A
Cuts at: 4452 4452
Size: 5276
BspMI ACCTGCnnnn'nnnn_
Cuts at: 531 552 789 1634 2326 2540 2842 3223 531
Size: 21 237 845 692 214 302 381 2584
Fragments arranged by size:
2584 845 692 381 302 237 214 21
BsrBI CCG'CTC
Cuts at: 50 3568 3622 5106 5271 50
Size: 3518 54 1484 165 55
Fragments arranged by size:
3518 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1943 3188 1943
Size: 1245 4031
BsrFI r'CCGG_y
Cuts at: 819 967 1090 1296 3274 3455 3664 819
Size: 148 123 206 1978 181 209 2431
Fragments arranged by size:
2431 1978 209 206 181 148 123
BsrGI T'GTAC_A
Cuts at: 1743 1743
Size: 5276
BssSI C'ACGA_G
Cuts at: 3547 3905 3547
Size: 358 4918
BstAPI GCAn_nnn'nTGC
Cuts at: 1242 2653 1242
Size: 1411 3865
BstBI TT'CG_AA
Cuts at: 3637 3637
Size: 5276
BstEII G'GTnAC_C
Cuts at: 1062 1062
Size: 5276
BstXI CCAn_nnnn'nTGG
Cuts at: 1464 1464
Size: 5276
BstYI r'GATC_y
Cuts at: 1 1169 1673 2301 2768 2791 3126 3372 4384
Size: 1168 504 628 467 23 335 246 1012
Cuts at: 4384 4470 4482 4828 1
Size: 86 12 346 449
Fragments arranged by size:
1168 1012 628 504 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1895 2048 2246 107
Size: 1788 153 198 3137
Fragments arranged by size:
3137 1788 198 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1050 3226 3407 3570 1050
Size: 2176 181 163 2756
Fragments arranged by size:
2756 2176 181 163
BtsI GCAGTG_nn'
Cuts at: 380 479 601 964 1346 1476 1721 2519 380
Size: 99 122 363 382 130 245 798 3137
Fragments arranged by size:
3137 798 382 363 245 130 122 99
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2153 2188 2153
Size: 35 5241
DraI TTT'AAA
Cuts at: 2275 2275
Size: 5276
DraIII CAC_nnn'GTG
Cuts at: 1190 2141 1190
Size: 951 4325
DrdI GACnn_nn'nnGTC
Cuts at: 2982 3840 2982
Size: 858 4418
EaeI y'GGCC_r
Cuts at: 540 741 969 993 2861 3035 3426 3453 4798
Size: 201 228 24 1868 174 391 27 1345
Cuts at: 4798 540
Size: 1018
Fragments arranged by size:
1868 1345 1018 391 228 201 174 27
24
EagI C'GGCC_G
Cuts at: 969 2861 969
Size: 1892 3384
EarI CTCTTCn'nnn_
Cuts at: 1526 3299 3509 1526
Size: 1773 210 3293
Fragments arranged by size:
3293 1773 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3484 3794 3940 5086 3484
Size: 310 146 1146 3674
Fragments arranged by size:
3674 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2288 2288
Size: 5276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 323 385 413 664 1120 1429 1507 1532 1561
Size: 62 28 251 456 309 78 25 29
Cuts at: 1561 1797 2011 2164 3101 3533 4280 4548 323
Size: 236 214 153 937 432 747 268 1051
Fragments arranged by size:
1051 937 747 456 432 309 268 251
236 214 153 78 62 29 28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 367 1262 367
Size: 895 4381
EcoO109I rG'GnC_Cy
Cuts at: 270 419 876 914 1280 2415 270
Size: 149 457 38 366 1135 3131
Fragments arranged by size:
3131 1135 457 366 149 38
EcoRI G'AATT_C
Cuts at: 2280 2280
Size: 5276
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1537 3295 1537
Size: 1758 3518
FspI TGC'GCA
Cuts at: 3057 3057
Size: 5276
HaeII r_GCGC'y
Cuts at: 135 288 933 2710 2958 3980 4959 135
Size: 153 645 1777 248 1022 979 452
Fragments arranged by size:
1777 1022 979 645 452 248 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 481 513 1148 1180 1256 1288 1599 1631 3118
Size: 32 635 32 76 32 311 32 1487
Cuts at: 3118 3148 3150 3180 481
Size: 30 2 30 2577
Fragments arranged by size:
2577 1487 635 311 76 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1415 2315 1415
Size: 900 4376
KasI G'GCGC_C
Cuts at: 284 2706 2954 284
Size: 2422 248 2606
Fragments arranged by size:
2606 2422 248
KpnI G_GTAC'C
Cuts at: 2296 2296
Size: 5276
MfeI C'AATT_G
Cuts at: 1727 1727
Size: 5276
MluI A'CGCG_T
Cuts at: 4709 4709
Size: 5276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1448 1940 3947 4131 1448
Size: 492 2007 184 2593
Fragments arranged by size:
2593 2007 492 184
MscI TGG'CCA
Cuts at: 542 743 995 3037 542
Size: 201 252 2042 2781
Fragments arranged by size:
2781 2042 252 201
MslI CAynn'nnrTG
Cuts at: 511 1250 1894 1975 3265 3392 511
Size: 739 644 81 1290 127 2395
Fragments arranged by size:
2395 1290 739 644 127 81
MspA1I CmG'CkG
Cuts at: 709 1478 1722 2269 2387 2701 3061 4074 4319
Size: 769 244 547 118 314 360 1013 245
Cuts at: 4319 709
Size: 1666
Fragments arranged by size:
1666 1013 769 547 360 314 245 244
118
NaeI GCC'GGC
Cuts at: 3457 3457
Size: 5276
NarI GG'CG_CC
Cuts at: 285 2707 2955 285
Size: 2422 248 2606
Fragments arranged by size:
2606 2422 248
NcoI C'CATG_G
Cuts at: 107 1895 2048 107
Size: 1788 153 3335
Fragments arranged by size:
3335 1788 153
NgoMIV G'CCGG_C
Cuts at: 3455 3455
Size: 5276
NspI r_CATG'y
Cuts at: 2173 2214 2329 2829 3736 2173
Size: 41 115 500 907 3713
Fragments arranged by size:
3713 907 500 115 41
PasI CC'CwG_GG
Cuts at: 210 1218 210
Size: 1008 4268
PciI A'CATG_T
Cuts at: 2169 2210 3732 2169
Size: 41 1522 3713
Fragments arranged by size:
3713 1522 41
PflMI CCAn_nnn'nTGG
Cuts at: 1775 1775
Size: 5276
PfoI T'CCnGG_A
Cuts at: 499 499
Size: 5276
PmeI GTTT'AAAC
Cuts at: 2275 2275
Size: 5276
PmlI CAC'GTG
Cuts at: 828 828
Size: 5276
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4415 4447 4415
Size: 32 5244
PpuMI rG'GwC_Cy
Cuts at: 419 876 1280 419
Size: 457 404 4415
Fragments arranged by size:
4415 457 404
PstI C_TGCA'G
Cuts at: 563 2323 3008 563
Size: 1760 685 2831
Fragments arranged by size:
2831 1760 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5276
PvuII CAG'CTG
Cuts at: 709 1722 2269 2701 3061 709
Size: 1013 547 432 360 2924
Fragments arranged by size:
2924 1013 547 432 360
RsrII CG'GwC_CG
Cuts at: 3471 3471
Size: 5276
SacI G_AGCT'C
Cuts at: 2290 2290
Size: 5276
SalI G'TCGA_C
Cuts at: 2313 2313
Size: 5276
SapI GCTCTTCn'nnn_
Cuts at: 3299 3509 3299
Size: 210 5066
SbfI CC_TGCA'GG
Cuts at: 2323 2323
Size: 5276
ScaI AGT'ACT
Cuts at: 670 4793 670
Size: 4123 1153
SfcI C'TryA_G
Cuts at: 33 559 2106 2319 3004 3997 4188 33
Size: 526 1547 213 685 993 191 1121
Fragments arranged by size:
1547 1121 993 685 526 213 191
SfoI GGC'GCC
Cuts at: 286 2708 2956 286
Size: 2422 248 2606
Fragments arranged by size:
2606 2422 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5276
SgrAI Cr'CCGG_yG
Cuts at: 1090 1090
Size: 5276
SmaI CCC'GGG
Cuts at: 2298 2298
Size: 5276
SmlI C'TyrA_G
Cuts at: 862 1603 2431 3346 3838 4100 862
Size: 741 828 915 492 262 2038
Fragments arranged by size:
2038 915 828 741 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5276
SphI G_CATG'C
Cuts at: 2329 2829 2329
Size: 500 4776
StuI AGG'CCT
Cuts at: 1000 1000
Size: 5276
StyI C'CwwG_G
Cuts at: 107 266 1457 1895 2048 2410 2764 107
Size: 159 1191 438 153 362 354 2619
Fragments arranged by size:
2619 1191 438 362 354 159 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 470 832 919 993 1262 1656 2859 2992 3451
Size: 362 87 74 269 394 1203 133 459
Cuts at: 3451 3660 470
Size: 209 2086
Fragments arranged by size:
2086 1203 459 394 362 269 209 133
87 74
TatI w'GTAC_w
Cuts at: 326 668 1743 4791 326
Size: 342 1075 3048 811
Fragments arranged by size:
3048 1075 811 342
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 445 477 1377 1409 1897 1929 4793 4825 445
Size: 32 900 32 488 32 2864 32 896
Fragments arranged by size:
2864 900 896 488 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 532 3073 532
Size: 2541 2735
XbaI T'CTAG_A
Cuts at: 2307 2307
Size: 5276
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1225 2771 1225
Size: 1546 3730
XhoI C'TCGA_G
Cuts at: 1603 1603
Size: 5276
XmaI C'CCGG_G
Cuts at: 2296 2296
Size: 5276
XmnI GAAnn'nnTTC
Cuts at: 1876 1876
Size: 5276
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AflII ApaI AscI AvrII BaeI BcgI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BssHII BstZ17I Bsu36I
ClaI EcoRV FseI FspAI HindIII HpaI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SnaBI SrfI SspI
SwaI ZraI