Restriction map for
pF1KB5718
(Circular) (Six-base) MAPSORT of: pF1KB5718.seq Check: 9819 from: 1 to: 5285
pF1KB5718 5285 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 2323 2323
Size: 5285
Acc65I G'GTAC_C
Cuts at: 2301 2301
Size: 5285
AclI AA'CG_TT
Cuts at: 5125 5125
Size: 5285
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 848 962 1581 3110 3542 4289 4557 848
Size: 114 619 1529 432 747 268 1576
Fragments arranged by size:
1576 1529 747 619 432 268 114
AflIII A'CryG_T
Cuts at: 1183 2637 3741 4718 1183
Size: 1454 1104 977 1750
Fragments arranged by size:
1750 1454 1104 977
AgeI A'CCGG_T
Cuts at: 3673 3673
Size: 5285
AleI CACnn'nnGTG
Cuts at: 1949 1949
Size: 5285
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1556 1588 1556
Size: 32 5253
AlwNI CAG_nnn'CTG
Cuts at: 1213 1636 2590 4157 1213
Size: 423 954 1567 2341
Fragments arranged by size:
2341 1567 954 423
ApaLI G'TGCA_C
Cuts at: 4055 4055
Size: 5285
ApoI r'AATT_y
Cuts at: 294 1706 2289 4638 4894 294
Size: 1412 583 2349 256 685
Fragments arranged by size:
2349 1412 685 583 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5285
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
AvaI C'yCGr_G
Cuts at: 1871 2216 2305 1871
Size: 345 89 4851
Fragments arranged by size:
4851 345 89
BamHI G'GATC_C
Cuts at: 642 2310 642
Size: 1668 3617
BanI G'GyrC_C
Cuts at: 2301 2715 2963 2998 2301
Size: 414 248 35 4588
Fragments arranged by size:
4588 414 248 35
BanII G_rGCy'C
Cuts at: 2299 3329 2299
Size: 1030 4255
BbeI G_GCGC'C
Cuts at: 2719 2967 2719
Size: 248 5037
BbsI GAAGACnn'nnnn_
Cuts at: 2000 2200 2000
Size: 200 5085
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1229 1263 1229
Size: 34 5251
BciVI GTATCCnnnnn_n'
Cuts at: 1201 1240 3183 3944 5285 1201
Size: 39 1943 761 1341 1201
Fragments arranged by size:
1943 1341 1201 761 39
BclI T'GATC_A
Cuts at: 258 2045 2805 258
Size: 1787 760 2738
Fragments arranged by size:
2738 1787 760
BfrBI ATG'CAT
Cuts at: 181 181
Size: 5285
BglII A'GATC_T
Cuts at: 1354 1846 2082 2800 4837 1354
Size: 492 236 718 2037 1802
Fragments arranged by size:
2037 1802 718 492 236
BlpI GC'TnA_GC
Cuts at: 2397 2397
Size: 5285
Bme1580I G_kGCm'C
Cuts at: 855 2910 3003 4059 855
Size: 2055 93 1056 2081
Fragments arranged by size:
2081 2055 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1834 2497 2578 2913 5057 1834
Size: 663 81 335 2144 2062
Fragments arranged by size:
2144 2062 663 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1886 3652 1886
Size: 1766 3519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 858 1520 245
Size: 613 662 4010
Fragments arranged by size:
4010 662 613
Bpu10I CC'TnA_GC
Cuts at: 2139 2139
Size: 5285
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 632 1298 1660 2461 3340 3832 4130 632
Size: 666 362 801 879 492 298 1787
Fragments arranged by size:
1787 879 801 666 492 362 298
BsaI GGTCTCn'nnnn_
Cuts at: 4989 4989
Size: 5285
BsaAI yAC'GTr
Cuts at: 850 1258 2119 2638 850
Size: 408 861 519 3497
Fragments arranged by size:
3497 861 519 408
BsaBI GATnn'nnATC
Cuts at: 6 2799 4836 6
Size: 2793 2037 455
Fragments arranged by size:
2793 2037 455
BsaHI Gr'CG_yC
Cuts at: 2716 2964 3666 5162 2716
Size: 248 702 1496 2839
Fragments arranged by size:
2839 1496 702 248
BsaWI w'CCGG_w
Cuts at: 133 380 2474 2995 3673 3947 4094 4505 133
Size: 247 2094 521 678 274 147 411 913
Fragments arranged by size:
2094 913 678 521 411 274 247 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 151 181 567 597 151
Size: 30 386 30 4839
Fragments arranged by size:
4839 386 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 204 424 427 575 1568 204
Size: 220 3 148 993 3921
Fragments arranged by size:
3921 993 220 148 3
BseYI C'CCAG_C
Cuts at: 2710 3468 4045 4541 2710
Size: 758 577 496 3454
Fragments arranged by size:
3454 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2827 2873 4081 104
Size: 2723 46 1208 1308
Fragments arranged by size:
2723 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2299 3077 3267 4059 2299
Size: 778 190 792 3525
Fragments arranged by size:
3525 792 778 190
BsmI GAATG_Cn'
Cuts at: 632 1571 632
Size: 939 4346
Bsp1286I G_dGCh'C
Cuts at: 855 2299 2910 3003 3077 3267 3329 4059 855
Size: 1444 611 93 74 190 62 730 2081
Fragments arranged by size:
2081 1444 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 133 4505 133
Size: 4372 913
BspHI T'CATG_A
Cuts at: 1161 4461 1161
Size: 3300 1985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 896 2335 2549 2851 3232 896
Size: 1439 214 302 381 2949
Fragments arranged by size:
2949 1439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 113 3577 3631 5115 5280 50
Size: 63 3464 54 1484 165 55
Fragments arranged by size:
3464 1484 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 753 3197 753
Size: 2444 2841
BsrFI r'CCGG_y
Cuts at: 3283 3464 3673 3283
Size: 181 209 4895
Fragments arranged by size:
4895 209 181
BssSI C'ACGA_G
Cuts at: 1334 3556 3914 1334
Size: 2222 358 2705
Fragments arranged by size:
2705 2222 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2662 2662
Size: 5285
BstBI TT'CG_AA
Cuts at: 3646 3646
Size: 5285
BstYI r'GATC_y
Cuts at: 1 228 474 642 1354 1846 2082 2310 2777
Size: 227 246 168 712 492 236 228 467
Cuts at: 2777 2800 3135 3381 4393 4479 4491 4837 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 712 492 467 449 346 335 246
246 236 228 227 168 86 23 12
BtgI C'CryG_G
Cuts at: 1661 1725 1661
Size: 64 5221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3235 3416 3579 3235
Size: 181 163 4941
Fragments arranged by size:
4941 181 163
BtsI GCAGTG_nn'
Cuts at: 216 1941 2139 2154 2528 216
Size: 1725 198 15 374 2973
Fragments arranged by size:
2973 1725 374 198 15
DraI TTT'AAA
Cuts at: 293 1286 1389 2284 293
Size: 993 103 895 3294
Fragments arranged by size:
3294 993 895 103
DrdI GACnn_nn'nnGTC
Cuts at: 2991 3849 2991
Size: 858 4427
EaeI y'GGCC_r
Cuts at: 213 1138 2870 3044 3435 3462 4807 213
Size: 925 1732 174 391 27 1345 691
Fragments arranged by size:
1732 1345 925 691 391 174 27
EagI C'GGCC_G
Cuts at: 2870 2870
Size: 5285
EarI CTCTTCn'nnn_
Cuts at: 181 735 1884 1949 2263 3308 3518 181
Size: 554 1149 65 314 1045 210 1948
Fragments arranged by size:
1948 1149 1045 554 314 210 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3493 3803 3949 5095 3493
Size: 310 146 1146 3683
Fragments arranged by size:
3683 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2297 2297
Size: 5285
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 848 858 962 1520 1581 3110 3542 4289
Size: 603 10 104 558 61 1529 432 747
Cuts at: 4289 4557 245
Size: 268 973
Fragments arranged by size:
1529 973 747 603 558 432 268 104
61 10
EcoNI CCTnn'n_nnAGG
Cuts at: 1238 1238
Size: 5285
EcoO109I rG'GnC_Cy
Cuts at: 2424 2424
Size: 5285
EcoRI G'AATT_C
Cuts at: 2289 2289
Size: 5285
EcoRV GAT'ATC
Cuts at: 366 366
Size: 5285
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3304 3304
Size: 5285
FspI TGC'GCA
Cuts at: 3066 3066
Size: 5285
HaeII r_GCGC'y
Cuts at: 1795 2719 2967 3989 4968 1795
Size: 924 248 1022 979 2112
Fragments arranged by size:
2112 1022 979 924 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 553 585 1540 1572 1838 1870 2187 2219 3127
Size: 32 955 32 266 32 317 32 908
Cuts at: 3127 3157 3159 3189 553
Size: 30 2 30 2649
Fragments arranged by size:
2649 955 908 317 266 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 304 870 1406 2184 2324 304
Size: 566 536 778 140 3265
Fragments arranged by size:
3265 778 566 536 140
KasI G'GCGC_C
Cuts at: 2715 2963 2715
Size: 248 5037
KpnI G_GTAC'C
Cuts at: 2305 2305
Size: 5285
MluI A'CGCG_T
Cuts at: 4718 4718
Size: 5285
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 487 1063 3956 4140 487
Size: 576 2893 184 1632
Fragments arranged by size:
2893 1632 576 184
MscI TGG'CCA
Cuts at: 3046 3046
Size: 5285
MslI CAynn'nnrTG
Cuts at: 1949 3274 3401 1949
Size: 1325 127 3833
Fragments arranged by size:
3833 1325 127
MspA1I CmG'CkG
Cuts at: 514 2396 2710 3070 4083 4328 514
Size: 1882 314 360 1013 245 1471
Fragments arranged by size:
1882 1471 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3466 3466
Size: 5285
NarI GG'CG_CC
Cuts at: 2716 2964 2716
Size: 248 5037
NcoI C'CATG_G
Cuts at: 1725 1725
Size: 5285
NdeI CA'TA_TG
Cuts at: 1589 1589
Size: 5285
NgoMIV G'CCGG_C
Cuts at: 3464 3464
Size: 5285
NsiI A_TGCA'T
Cuts at: 183 183
Size: 5285
NspI r_CATG'y
Cuts at: 709 1015 2338 2838 3745 709
Size: 306 1323 500 907 2249
Fragments arranged by size:
2249 1323 907 500 306
PciI A'CATG_T
Cuts at: 3741 3741
Size: 5285
PflMI CCAn_nnn'nTGG
Cuts at: 1045 1045
Size: 5285
PfoI T'CCnGG_A
Cuts at: 1536 1536
Size: 5285
PmeI GTTT'AAAC
Cuts at: 2284 2284
Size: 5285
PmlI CAC'GTG
Cuts at: 850 850
Size: 5285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4424 4456 4424
Size: 32 5253
PsiI TTA'TAA
Cuts at: 884 884
Size: 5285
PstI C_TGCA'G
Cuts at: 2332 3017 2332
Size: 685 4600
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5285
PvuII CAG'CTG
Cuts at: 514 2710 3070 514
Size: 2196 360 2729
Fragments arranged by size:
2729 2196 360
RsrII CG'GwC_CG
Cuts at: 3480 3480
Size: 5285
SacI G_AGCT'C
Cuts at: 2299 2299
Size: 5285
SalI G'TCGA_C
Cuts at: 2322 2322
Size: 5285
SapI GCTCTTCn'nnn_
Cuts at: 3308 3518 3308
Size: 210 5075
SbfI CC_TGCA'GG
Cuts at: 2332 2332
Size: 5285
ScaI AGT'ACT
Cuts at: 939 4802 939
Size: 3863 1422
SexAI A'CCwGG_T
Cuts at: 1044 1044
Size: 5285
SfcI C'TryA_G
Cuts at: 33 1235 2328 3013 4006 4197 33
Size: 1202 1093 685 993 191 1121
Fragments arranged by size:
1202 1121 1093 993 685 191
SfoI GGC'GCC
Cuts at: 2717 2965 2717
Size: 248 5037
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5285
SmaI CCC'GGG
Cuts at: 2218 2307 2218
Size: 89 5196
SmlI C'TyrA_G
Cuts at: 647 1313 1675 1871 2440 3355 3847 4109 647
Size: 666 362 196 569 915 492 262 1823
Fragments arranged by size:
1823 915 666 569 492 362 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5285
SphI G_CATG'C
Cuts at: 709 2338 2838 709
Size: 1629 500 3156
Fragments arranged by size:
3156 1629 500
SspI AAT'ATT
Cuts at: 247 247
Size: 5285
StyI C'CwwG_G
Cuts at: 1088 1725 2198 2419 2773 1088
Size: 637 473 221 354 3600
Fragments arranged by size:
3600 637 473 354 221
SwaI ATTT'AAAT
Cuts at: 293 293
Size: 5285
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 568 1181 2868 3001 3460 3669 568
Size: 613 1687 133 459 209 2184
Fragments arranged by size:
2184 1687 613 459 209 133
TatI w'GTAC_w
Cuts at: 937 4800 937
Size: 3863 1422
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 975 1007 4802 4834 975
Size: 32 3795 32 1426
Fragments arranged by size:
3795 1426 32 32
Tth111I GACn'n_nGTC
Cuts at: 3082 3082
Size: 5285
XbaI T'CTAG_A
Cuts at: 2316 2316
Size: 5285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 793 1275 2780 793
Size: 482 1505 3298
Fragments arranged by size:
3298 1505 482
XhoI C'TCGA_G
Cuts at: 1871 1871
Size: 5285
XmaI C'CCGG_G
Cuts at: 2216 2305 2216
Size: 89 5196
XmnI GAAnn'nnTTC
Cuts at: 1419 1505 1743 1419
Size: 86 238 4961
Fragments arranged by size:
4961 238 86
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PfoI PmeI PmlI PpiI PsiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII FseI FspAI HindIII HpaI MfeI NheI
NotI NruI PacI PasI PpuMI PshAI PspOMI PspXI
PsrI SacII SanDI SfiI SgrAI SnaBI SrfI StuI
ZraI