Restriction map for
pF1KB5733
(Circular) (Six-base) MAPSORT of: pF1KB5733.seq Check: 6736 from: 1 to: 5495
pF1KB5733 5495 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 393 2252 393
Size: 1859 3636
AccI GT'mk_AC
Cuts at: 2533 2533
Size: 5495
Acc65I G'GTAC_C
Cuts at: 473 2511 473
Size: 2038 3457
AclI AA'CG_TT
Cuts at: 5335 5335
Size: 5495
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2420 3320 3752 4499 4767 2420
Size: 900 432 747 268 3148
Fragments arranged by size:
3148 900 747 432 268
AflIII A'CryG_T
Cuts at: 203 431 2847 3951 4928 203
Size: 228 2416 1104 977 770
Fragments arranged by size:
2416 1104 977 770 228
AgeI A'CCGG_T
Cuts at: 3883 3883
Size: 5495
AlwNI CAG_nnn'CTG
Cuts at: 393 1216 1865 2027 2800 4367 393
Size: 823 649 162 773 1567 1521
Fragments arranged by size:
1567 1521 823 773 649 162
ApaI G_GGCC'C
Cuts at: 874 874
Size: 5495
ApaLI G'TGCA_C
Cuts at: 1185 4265 1185
Size: 3080 2415
ApoI r'AATT_y
Cuts at: 2499 4848 5104 2499
Size: 2349 256 2890
Fragments arranged by size:
2890 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5495
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
AvaI C'yCGr_G
Cuts at: 874 984 1501 2515 874
Size: 110 517 1014 3854
Fragments arranged by size:
3854 1014 517 110
BamHI G'GATC_C
Cuts at: 1731 2520 1731
Size: 789 4706
BanI G'GyrC_C
Cuts at: 473 1204 1402 1636 1822 2511 2925 3173 3208
Size: 731 198 234 186 689 414 248 35
Cuts at: 3208 473
Size: 2760
Fragments arranged by size:
2760 731 689 414 248 234 198 186
35
BanII G_rGCy'C
Cuts at: 446 874 967 1481 1541 2509 3539 446
Size: 428 93 514 60 968 1030 2402
Fragments arranged by size:
2402 1030 968 514 428 93 60
BbeI G_GCGC'C
Cuts at: 1406 2929 3177 1406
Size: 1523 248 3724
Fragments arranged by size:
3724 1523 248
BbsI GAAGACnn'nnnn_
Cuts at: 186 1519 2283 2295 186
Size: 1333 764 12 3386
Fragments arranged by size:
3386 1333 764 12
BbvCI CC'TCA_GC
Cuts at: 115 464 1209 2086 2266 115
Size: 349 745 877 180 3344
Fragments arranged by size:
3344 877 745 349 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1155 1189 2164 2198 1155
Size: 34 975 34 4452
Fragments arranged by size:
4452 975 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3393 4154 5495 3393
Size: 761 1341 3393
Fragments arranged by size:
3393 1341 761
BclI T'GATC_A
Cuts at: 3015 3015
Size: 5495
BglI GCCn_nnn'nGGC
Cuts at: 922 1411 1634 1955 2184 922
Size: 489 223 321 229 4233
Fragments arranged by size:
4233 489 321 229 223
BglII A'GATC_T
Cuts at: 284 3010 5047 284
Size: 2726 2037 732
Fragments arranged by size:
2726 2037 732
BlpI GC'TnA_GC
Cuts at: 2607 2607
Size: 5495
Bme1580I G_kGCm'C
Cuts at: 542 874 1189 1207 1639 2422 3120 3213 4269
Size: 332 315 18 432 783 698 93 1056
Cuts at: 4269 542
Size: 1768
Fragments arranged by size:
1768 1056 783 698 432 332 315 93
18
BmgBI CAC'GTC
Cuts at: 432 1880 2004 432
Size: 1448 124 3923
Fragments arranged by size:
3923 1448 124
BmrI ACTGGGnnnn_n'
Cuts at: 1347 1707 2707 2788 3123 5267 1347
Size: 360 1000 81 335 2144 1575
Fragments arranged by size:
2144 1575 1000 360 335 81
BmtI G_CTAG'C
Cuts at: 169 169
Size: 5495
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 395 837 1122 1752 3862 395
Size: 442 285 630 2110 2028
Fragments arranged by size:
2110 2028 630 442 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 1273 1579 1835 1937 245
Size: 1028 306 256 102 3803
Fragments arranged by size:
3803 1028 306 256 102
Bpu10I CC'TnA_GC
Cuts at: 115 179 464 1209 1224 1568 2086 2266 115
Size: 64 285 745 15 344 518 180 3344
Fragments arranged by size:
3344 745 518 344 285 180 64 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 365 2671 3550 4042 4340 365
Size: 2306 879 492 298 1520
Fragments arranged by size:
2306 1520 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 558 2002 5199 558
Size: 1444 3197 854
Fragments arranged by size:
3197 1444 854
BsaAI yAC'GTr
Cuts at: 206 2848 206
Size: 2642 2853
BsaBI GATnn'nnATC
Cuts at: 6 1730 3009 5046 6
Size: 1724 1279 2037 455
Fragments arranged by size:
2037 1724 1279 455
BsaHI Gr'CG_yC
Cuts at: 1403 2926 3174 3876 5372 1403
Size: 1523 248 702 1496 1526
Fragments arranged by size:
1526 1523 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2684 3205 3883 4157 4304 4715 2684
Size: 521 678 274 147 411 3464
Fragments arranged by size:
3464 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2407 2437 2407
Size: 30 5465
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 332 934 2428 332
Size: 602 1494 3399
Fragments arranged by size:
3399 1494 602
BseYI C'CCAG_C
Cuts at: 231 653 825 866 960 1445 2095 2920 3678
Size: 422 172 41 94 485 650 825 758
Cuts at: 3678 4255 4751 231
Size: 577 496 975
Fragments arranged by size:
975 825 758 650 577 496 485 422
172 94 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1906 1906
Size: 5495
BsiEI CG_ry'CG
Cuts at: 104 3037 3083 4291 104
Size: 2933 46 1208 1308
Fragments arranged by size:
2933 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1565 2509 3287 3477 4269 1189
Size: 376 944 778 190 792 2415
Fragments arranged by size:
2415 944 792 778 376 190
Bsp1286I G_dGCh'C
Cuts at: 446 542 874 967 1189 1207 1481 1541 1565
Size: 96 332 93 222 18 274 60 24
Cuts at: 1565 1639 2422 2509 3120 3213 3287 3477 3539
Size: 74 783 87 611 93 74 190 62
Cuts at: 3539 4269 446
Size: 730 1672
Fragments arranged by size:
1672 783 730 611 332 274 222 190
96 93 93 87 74 74 62 60
24 18
BspEI T'CCGG_A
Cuts at: 4715 4715
Size: 5495
BspHI T'CATG_A
Cuts at: 1706 4671 1706
Size: 2965 2530
BspMI ACCTGCnnnn'nnnn_
Cuts at: 393 2252 2545 2759 3061 3442 393
Size: 1859 293 214 302 381 2446
Fragments arranged by size:
2446 1859 381 302 293 214
BsrBI CCG'CTC
Cuts at: 50 1643 3787 3841 5325 5490 50
Size: 1593 2144 54 1484 165 55
Fragments arranged by size:
2144 1593 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1238 3407 1238
Size: 2169 3326
BsrFI r'CCGG_y
Cuts at: 362 1231 2067 2260 3493 3674 3883 362
Size: 869 836 193 1233 181 209 1974
Fragments arranged by size:
1974 1233 869 836 209 193 181
BsrGI T'GTAC_A
Cuts at: 2354 2354
Size: 5495
BssSI C'ACGA_G
Cuts at: 3766 4124 3766
Size: 358 5137
BstAPI GCAn_nnn'nTGC
Cuts at: 175 1865 2217 2872 175
Size: 1690 352 655 2798
Fragments arranged by size:
2798 1690 655 352
BstBI TT'CG_AA
Cuts at: 3856 3856
Size: 5495
BstXI CCAn_nnnn'nTGG
Cuts at: 249 1353 1378 1620 1653 249
Size: 1104 25 242 33 4091
Fragments arranged by size:
4091 1104 242 33 25
BstYI r'GATC_y
Cuts at: 1 284 1731 2520 2987 3010 3345 3591 4603
Size: 283 1447 789 467 23 335 246 1012
Cuts at: 4603 4689 4701 5047 1
Size: 86 12 346 449
Fragments arranged by size:
1447 1012 789 467 449 346 335 283
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1832 2249 1832
Size: 417 5078
BtgI C'CryG_G
Cuts at: 107 352 933 1087 1406 1599 1631 2360 2367
Size: 245 581 154 319 193 32 729 7
Cuts at: 2367 107
Size: 3235
Fragments arranged by size:
3235 729 581 319 245 193 154 32
7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3445 3626 3789 3445
Size: 181 163 5151
Fragments arranged by size:
5151 181 163
BtsI GCAGTG_nn'
Cuts at: 475 815 1140 1866 1944 2738 475
Size: 340 325 726 78 794 3232
Fragments arranged by size:
3232 794 726 340 325 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 370 405 1056 1091 370
Size: 35 651 35 4774
Fragments arranged by size:
4774 651 35 35
DraI TTT'AAA
Cuts at: 2494 2494
Size: 5495
DraIII CAC_nnn'GTG
Cuts at: 698 700 1296 698
Size: 2 596 4897
Fragments arranged by size:
4897 596 2
DrdI GACnn_nn'nnGTC
Cuts at: 330 1878 3201 4059 330
Size: 1548 1323 858 1766
Fragments arranged by size:
1766 1548 1323 858
EaeI y'GGCC_r
Cuts at: 239 722 1118 1610 2258 3080 3254 3645 3672
Size: 483 396 492 648 822 174 391 27
Cuts at: 3672 5017 239
Size: 1345 717
Fragments arranged by size:
1345 822 717 648 492 483 396 391
174 27
EagI C'GGCC_G
Cuts at: 3080 3080
Size: 5495
EarI CTCTTCn'nnn_
Cuts at: 1924 2429 3518 3728 1924
Size: 505 1089 210 3691
Fragments arranged by size:
3691 1089 505 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3703 4013 4159 5305 3703
Size: 310 146 1146 3893
Fragments arranged by size:
3893 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2507 2507
Size: 5495
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 245 1273 1579 1835 1937 2420 3320 3752 4499
Size: 1028 306 256 102 483 900 432 747
Cuts at: 4499 4767 245
Size: 268 973
Fragments arranged by size:
1028 973 900 747 483 432 306 268
256 102
EcoNI CCTnn'n_nnAGG
Cuts at: 916 2248 916
Size: 1332 4163
EcoO109I rG'GnC_Cy
Cuts at: 119 498 642 870 871 1749 2634 119
Size: 379 144 228 1 878 885 2980
Fragments arranged by size:
2980 885 878 379 228 144 1
EcoRI G'AATT_C
Cuts at: 2499 2499
Size: 5495
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3514 3514
Size: 5495
FseI GG_CCGG'CC
Cuts at: 2071 2264 2071
Size: 193 5302
FspI TGC'GCA
Cuts at: 217 781 3276 217
Size: 564 2495 2436
Fragments arranged by size:
2495 2436 564
FspAI rTGC'GCAy
Cuts at: 217 781 217
Size: 564 4931
HaeII r_GCGC'y
Cuts at: 1067 1406 2486 2929 3177 4199 5178 1067
Size: 339 1080 443 248 1022 979 1384
Fragments arranged by size:
1384 1080 1022 979 443 339 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2407 2439 3337 3367 3369 3399 2407
Size: 32 898 30 2 30 4503
Fragments arranged by size:
4503 898 32 30 30 2
HincII GTy'rAC
Cuts at: 2534 2534
Size: 5495
KasI G'GCGC_C
Cuts at: 1402 2925 3173 1402
Size: 1523 248 3724
Fragments arranged by size:
3724 1523 248
KpnI G_GTAC'C
Cuts at: 477 2515 477
Size: 2038 3457
MluI A'CGCG_T
Cuts at: 4928 4928
Size: 5495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4166 4350 4166
Size: 184 5311
MscI TGG'CCA
Cuts at: 241 724 1120 1612 3256 241
Size: 483 396 492 1644 2480
Fragments arranged by size:
2480 1644 492 483 396
MslI CAynn'nnrTG
Cuts at: 670 1325 1376 3484 3611 670
Size: 655 51 2108 127 2554
Fragments arranged by size:
2554 2108 655 127 51
MspA1I CmG'CkG
Cuts at: 172 235 354 495 866 1013 1449 2090 2270
Size: 63 119 141 371 147 436 641 180
Cuts at: 2270 2319 2606 2920 3280 4293 4538 172
Size: 49 287 314 360 1013 245 1129
Fragments arranged by size:
1129 1013 641 436 371 360 314 287
245 180 147 141 119 63 49
NaeI GCC'GGC
Cuts at: 364 1233 2069 2262 3676 364
Size: 869 836 193 1414 2183
Fragments arranged by size:
2183 1414 869 836 193
NarI GG'CG_CC
Cuts at: 1403 2926 3174 1403
Size: 1523 248 3724
Fragments arranged by size:
3724 1523 248
NcoI C'CATG_G
Cuts at: 107 1406 1631 2360 107
Size: 1299 225 729 3242
Fragments arranged by size:
3242 1299 729 225
NdeI CA'TA_TG
Cuts at: 1377 1377
Size: 5495
NgoMIV G'CCGG_C
Cuts at: 362 1231 2067 2260 3674 362
Size: 869 836 193 1414 2183
Fragments arranged by size:
2183 1414 869 836 193
NheI G'CTAG_C
Cuts at: 165 165
Size: 5495
NspI r_CATG'y
Cuts at: 786 1287 2060 2548 3048 3955 786
Size: 501 773 488 500 907 2326
Fragments arranged by size:
2326 907 773 501 500 488
PciI A'CATG_T
Cuts at: 3951 3951
Size: 5495
PflMI CCAn_nnn'nTGG
Cuts at: 206 1337 206
Size: 1131 4364
PmeI GTTT'AAAC
Cuts at: 2494 2494
Size: 5495
PmlI CAC'GTG
Cuts at: 206 206
Size: 5495
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4634 4666 4634
Size: 32 5463
PpuMI rG'GwC_Cy
Cuts at: 498 642 1749 498
Size: 144 1107 4244
Fragments arranged by size:
4244 1107 144
PspOMI G'GGCC_C
Cuts at: 870 870
Size: 5495
PspXI vC'TCGA_Gb
Cuts at: 1501 1501
Size: 5495
PstI C_TGCA'G
Cuts at: 371 2318 2542 3227 371
Size: 1947 224 685 2639
Fragments arranged by size:
2639 1947 685 224
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5495
PvuII CAG'CTG
Cuts at: 172 866 1013 2090 2270 2319 2920 3280 172
Size: 694 147 1077 180 49 601 360 2387
Fragments arranged by size:
2387 1077 694 601 360 180 147 49
RsrII CG'GwC_CG
Cuts at: 3690 3690
Size: 5495
SacI G_AGCT'C
Cuts at: 2509 2509
Size: 5495
SacII CC_GC'GG
Cuts at: 355 355
Size: 5495
SalI G'TCGA_C
Cuts at: 2532 2532
Size: 5495
SapI GCTCTTCn'nnn_
Cuts at: 1924 3518 3728 1924
Size: 1594 210 3691
Fragments arranged by size:
3691 1594 210
SbfI CC_TGCA'GG
Cuts at: 2542 2542
Size: 5495
ScaI AGT'ACT
Cuts at: 5012 5012
Size: 5495
SexAI A'CCwGG_T
Cuts at: 1292 1619 1292
Size: 327 5168
SfcI C'TryA_G
Cuts at: 33 367 855 1128 1986 2314 2538 3223 4216
Size: 334 488 273 858 328 224 685 993
Cuts at: 4216 4407 33
Size: 191 1121
Fragments arranged by size:
1121 993 858 685 488 334 328 273
224 191
SfoI GGC'GCC
Cuts at: 1404 2927 3175 1404
Size: 1523 248 3724
Fragments arranged by size:
3724 1523 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5495
SmaI CCC'GGG
Cuts at: 2517 2517
Size: 5495
SmlI C'TyrA_G
Cuts at: 380 1501 2650 3565 4057 4319 380
Size: 1121 1149 915 492 262 1556
Fragments arranged by size:
1556 1149 1121 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5495
SphI G_CATG'C
Cuts at: 786 2548 3048 786
Size: 1762 500 3233
Fragments arranged by size:
3233 1762 500
StuI AGG'CCT
Cuts at: 925 1102 1891 925
Size: 177 789 4529
Fragments arranged by size:
4529 789 177
StyI C'CwwG_G
Cuts at: 107 155 1406 1631 2108 2360 2629 2983 107
Size: 48 1251 225 477 252 269 354 2619
Fragments arranged by size:
2619 1251 477 354 269 252 225 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 123 3078 3211 3670 3879 123
Size: 2955 133 459 209 1739
Fragments arranged by size:
2955 1739 459 209 133
TatI w'GTAC_w
Cuts at: 384 2354 5010 384
Size: 1970 2656 869
Fragments arranged by size:
2656 1970 869
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5012 5044 5012
Size: 32 5463
Tth111I GACn'n_nGTC
Cuts at: 3292 3292
Size: 5495
XbaI T'CTAG_A
Cuts at: 2526 2526
Size: 5495
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1350 1413 2990 1350
Size: 63 1577 3855
Fragments arranged by size:
3855 1577 63
XhoI C'TCGA_G
Cuts at: 1501 1501
Size: 5495
XmaI C'CCGG_G
Cuts at: 2515 2515
Size: 5495
XmnI GAAnn'nnTTC
Cuts at: 193 193
Size: 5495
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BfrBI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I ClaI EcoRV HindIII HpaI MfeI NotI NruI
NsiI PacI PasI PfoI PshAI PsiI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI