Restriction map for
pF1KB5734
(Circular) (Six-base) MAPSORT of: pF1KB5734.seq Check: 9273 from: 1 to: 5318
pF1KB5734 5318 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AatII G_ACGT'C
Cuts at: 2139 2139
Size: 5318
AccI GT'mk_AC
Cuts at: 758 2356 758
Size: 1598 3720
Acc65I G'GTAC_C
Cuts at: 2334 2334
Size: 5318
AclI AA'CG_TT
Cuts at: 5158 5158
Size: 5318
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 727 1655 3143 3575 4322 4590 571
Size: 156 928 1488 432 747 268 1299
Fragments arranged by size:
1488 1299 928 747 432 268 156
AflIII A'CryG_T
Cuts at: 725 915 2670 3774 4751 725
Size: 190 1755 1104 977 1292
Fragments arranged by size:
1755 1292 1104 977 190
AgeI A'CCGG_T
Cuts at: 3706 3706
Size: 5318
AleI CACnn'nnGTG
Cuts at: 667 918 667
Size: 251 5067
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1918 1918
Size: 5318
AlwNI CAG_nnn'CTG
Cuts at: 804 1551 2623 4190 804
Size: 747 1072 1567 1932
Fragments arranged by size:
1932 1567 1072 747
ApaI G_GGCC'C
Cuts at: 2275 2275
Size: 5318
ApaLI G'TGCA_C
Cuts at: 4088 4088
Size: 5318
ApoI r'AATT_y
Cuts at: 454 1182 1786 2322 4671 4927 454
Size: 728 604 536 2349 256 845
Fragments arranged by size:
2349 845 728 604 536 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5318
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5318
AvaI C'yCGr_G
Cuts at: 2338 2338
Size: 5318
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2027 2060 2027
Size: 33 5285
BamHI G'GATC_C
Cuts at: 491 2343 491
Size: 1852 3466
BanI G'GyrC_C
Cuts at: 1013 2334 2748 2996 3031 1013
Size: 1321 414 248 35 3300
Fragments arranged by size:
3300 1321 414 248 35
BanII G_rGCy'C
Cuts at: 1361 2275 2332 3362 1361
Size: 914 57 1030 3317
Fragments arranged by size:
3317 1030 914 57
BbeI G_GCGC'C
Cuts at: 2752 3000 2752
Size: 248 5070
BbsI GAAGACnn'nnnn_
Cuts at: 449 1171 449
Size: 722 4596
BbvCI CC'TCA_GC
Cuts at: 574 574
Size: 5318
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 826 860 826
Size: 34 5284
BciVI GTATCCnnnnn_n'
Cuts at: 3216 3977 5318 3216
Size: 761 1341 3216
Fragments arranged by size:
3216 1341 761
BclI T'GATC_A
Cuts at: 2838 2838
Size: 5318
BfrBI ATG'CAT
Cuts at: 1923 1923
Size: 5318
BglII A'GATC_T
Cuts at: 1850 2833 4870 1850
Size: 983 2037 2298
Fragments arranged by size:
2298 2037 983
BlpI GC'TnA_GC
Cuts at: 2430 2430
Size: 5318
Bme1580I G_kGCm'C
Cuts at: 2275 2943 3036 4092 2275
Size: 668 93 1056 3501
Fragments arranged by size:
3501 1056 668 93
BmrI ACTGGGnnnn_n'
Cuts at: 131 1109 1645 2530 2611 2946 5090 131
Size: 978 536 885 81 335 2144 359
Fragments arranged by size:
2144 978 885 536 359 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3685 3685
Size: 5318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 715 1849 2104 445
Size: 270 1134 255 3659
Fragments arranged by size:
3659 1134 270 255
Bpu10I CC'TnA_GC
Cuts at: 316 574 316
Size: 258 5060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 620 1549 2107 2328 2494 3373 3865 4163 620
Size: 929 558 221 166 879 492 298 1775
Fragments arranged by size:
1775 929 879 558 492 298 221 166
BsaI GGTCTCn'nnnn_
Cuts at: 1429 1950 5022 1429
Size: 521 3072 1725
Fragments arranged by size:
3072 1725 521
BsaAI yAC'GTr
Cuts at: 2671 2671
Size: 5318
BsaBI GATnn'nnATC
Cuts at: 6 976 2832 4869 6
Size: 970 1856 2037 455
Fragments arranged by size:
2037 1856 970 455
BsaHI Gr'CG_yC
Cuts at: 2136 2221 2749 2997 3699 5195 2136
Size: 85 528 248 702 1496 2259
Fragments arranged by size:
2259 1496 702 528 248 85
BsaWI w'CCGG_w
Cuts at: 974 1134 1740 2507 3028 3706 3980 4127 4538
Size: 160 606 767 521 678 274 147 411
Cuts at: 4538 974
Size: 1754
Fragments arranged by size:
1754 767 678 606 521 411 274 160
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 944 1544 1846 944
Size: 600 302 4416
Fragments arranged by size:
4416 600 302
BseYI C'CCAG_C
Cuts at: 149 655 687 2743 3501 4078 4574 149
Size: 506 32 2056 758 577 496 893
Fragments arranged by size:
2056 893 758 577 506 496 32
BsiEI CG_ry'CG
Cuts at: 104 957 2860 2906 4114 104
Size: 853 1903 46 1208 1308
Fragments arranged by size:
1903 1308 1208 853 46
BsiHKAI G_wGCw'C
Cuts at: 1192 1289 2155 2332 3110 3300 4092 1192
Size: 97 866 177 778 190 792 2418
Fragments arranged by size:
2418 866 792 778 190 177 97
BsmI GAATG_Cn'
Cuts at: 2133 2133
Size: 5318
BsmBI CGTCTCn'nnnn_
Cuts at: 1403 1403
Size: 5318
Bsp1286I G_dGCh'C
Cuts at: 1192 1289 1361 2155 2275 2332 2943 3036 3110
Size: 97 72 794 120 57 611 93 74
Cuts at: 3110 3300 3362 4092 1192
Size: 190 62 730 2418
Fragments arranged by size:
2418 794 730 611 190 120 97 93
74 72 62 57
BspEI T'CCGG_A
Cuts at: 974 1740 4538 974
Size: 766 2798 1754
Fragments arranged by size:
2798 1754 766
BspHI T'CATG_A
Cuts at: 4494 4494
Size: 5318
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2368 2582 2884 3265 2368
Size: 214 302 381 4421
Fragments arranged by size:
4421 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2285 3610 3664 5148 5313 50
Size: 2235 1325 54 1484 165 55
Fragments arranged by size:
2235 1484 1325 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3230 3230
Size: 5318
BsrFI r'CCGG_y
Cuts at: 3316 3497 3706 3316
Size: 181 209 4928
Fragments arranged by size:
4928 209 181
BsrGI T'GTAC_A
Cuts at: 2227 2227
Size: 5318
BssSI C'ACGA_G
Cuts at: 3589 3947 3589
Size: 358 4960
BstAPI GCAn_nnn'nTGC
Cuts at: 770 1284 2695 770
Size: 514 1411 3393
Fragments arranged by size:
3393 1411 514
BstBI TT'CG_AA
Cuts at: 1784 3679 1784
Size: 1895 3423
BstXI CCAn_nnnn'nTGG
Cuts at: 1122 2268 1122
Size: 1146 4172
BstYI r'GATC_y
Cuts at: 1 491 971 977 1025 1559 1850 1869 2343
Size: 490 480 6 48 534 291 19 474
Cuts at: 2343 2810 2833 3168 3414 4426 4512 4524 4870
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4870 1
Size: 449
Fragments arranged by size:
1012 534 490 480 474 467 449 346
335 291 246 86 48 23 19 12
6
Bsu36I CC'TnA_GG
Cuts at: 1992 1992
Size: 5318
BtgI C'CryG_G
Cuts at: 107 1169 1962 107
Size: 1062 793 3463
Fragments arranged by size:
3463 1062 793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1881 3268 3449 3612 1881
Size: 1387 181 163 3587
Fragments arranged by size:
3587 1387 181 163
BtsI GCAGTG_nn'
Cuts at: 351 627 673 1907 2561 351
Size: 276 46 1234 654 3108
Fragments arranged by size:
3108 1234 654 276 46
ClaI AT'CG_AT
Cuts at: 1602 1713 1602
Size: 111 5207
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 365 400 625 660 365
Size: 35 225 35 5023
Fragments arranged by size:
5023 225 35 35
DraI TTT'AAA
Cuts at: 1181 2317 1181
Size: 1136 4182
DrdI GACnn_nn'nnGTC
Cuts at: 904 3024 3882 904
Size: 2120 858 2340
Fragments arranged by size:
2340 2120 858
EaeI y'GGCC_r
Cuts at: 110 436 2144 2903 3077 3468 3495 4840 110
Size: 326 1708 759 174 391 27 1345 588
Fragments arranged by size:
1708 1345 759 588 391 326 174 27
EagI C'GGCC_G
Cuts at: 2903 2903
Size: 5318
EarI CTCTTCn'nnn_
Cuts at: 211 3341 3551 211
Size: 3130 210 1978
Fragments arranged by size:
3130 1978 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3526 3836 3982 5128 3526
Size: 310 146 1146 3716
Fragments arranged by size:
3716 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2330 2330
Size: 5318
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 571 715 727 1655 1849 2104 3143 3575
Size: 126 144 12 928 194 255 1039 432
Cuts at: 3575 4322 4590 445
Size: 747 268 1173
Fragments arranged by size:
1173 1039 928 747 432 268 255 194
144 126 12
EcoNI CCTnn'n_nnAGG
Cuts at: 424 1336 1352 424
Size: 912 16 4390
Fragments arranged by size:
4390 912 16
EcoO109I rG'GnC_Cy
Cuts at: 123 2457 123
Size: 2334 2984
EcoRI G'AATT_C
Cuts at: 454 2322 454
Size: 1868 3450
EcoRV GAT'ATC
Cuts at: 1971 2028 1971
Size: 57 5261
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3337 3337
Size: 5318
FspI TGC'GCA
Cuts at: 3099 3099
Size: 5318
HaeII r_GCGC'y
Cuts at: 951 2752 3000 4022 5001 951
Size: 1801 248 1022 979 1268
Fragments arranged by size:
1801 1268 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1591 1623 1706 1738 3160 3190 3192 3222 1591
Size: 32 83 32 1422 30 2 30 3687
Fragments arranged by size:
3687 1422 83 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2357 2357
Size: 5318
KasI G'GCGC_C
Cuts at: 2748 2996 2748
Size: 248 5070
KpnI G_GTAC'C
Cuts at: 2338 2338
Size: 5318
MfeI C'AATT_G
Cuts at: 2093 2093
Size: 5318
MluI A'CGCG_T
Cuts at: 4751 4751
Size: 5318
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1958 3989 4173 1958
Size: 2031 184 3103
Fragments arranged by size:
3103 2031 184
MscI TGG'CCA
Cuts at: 112 2146 3079 112
Size: 2034 933 2351
Fragments arranged by size:
2351 2034 933
MslI CAynn'nnrTG
Cuts at: 667 918 1061 1900 2266 3307 3434 667
Size: 251 143 839 366 1041 127 2551
Fragments arranged by size:
2551 1041 839 366 251 143 127
MspA1I CmG'CkG
Cuts at: 153 959 1866 2429 2743 3103 4116 4361 153
Size: 806 907 563 314 360 1013 245 1110
Fragments arranged by size:
1110 1013 907 806 563 360 314 245
NaeI GCC'GGC
Cuts at: 3499 3499
Size: 5318
NarI GG'CG_CC
Cuts at: 2749 2997 2749
Size: 248 5070
NcoI C'CATG_G
Cuts at: 107 1169 1962 107
Size: 1062 793 3463
Fragments arranged by size:
3463 1062 793
NdeI CA'TA_TG
Cuts at: 1595 1595
Size: 5318
NgoMIV G'CCGG_C
Cuts at: 3497 3497
Size: 5318
NsiI A_TGCA'T
Cuts at: 1925 1925
Size: 5318
NspI r_CATG'y
Cuts at: 729 919 1820 1923 2371 2871 3778 729
Size: 190 901 103 448 500 907 2269
Fragments arranged by size:
2269 907 901 500 448 190 103
PasI CC'CwG_GG
Cuts at: 1488 1488
Size: 5318
PciI A'CATG_T
Cuts at: 725 915 3774 725
Size: 190 2859 2269
Fragments arranged by size:
2859 2269 190
PflMI CCAn_nnn'nTGG
Cuts at: 1940 1940
Size: 5318
PfoI T'CCnGG_A
Cuts at: 494 1079 494
Size: 585 4733
PmeI GTTT'AAAC
Cuts at: 2317 2317
Size: 5318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 4457
Size: 32 5286
PshAI GACnn'nnGTC
Cuts at: 959 959
Size: 5318
PspOMI G'GGCC_C
Cuts at: 2271 2271
Size: 5318
PstI C_TGCA'G
Cuts at: 2365 3050 2365
Size: 685 4633
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5318
PvuII CAG'CTG
Cuts at: 153 2743 3103 153
Size: 2590 360 2368
Fragments arranged by size:
2590 2368 360
RsrII CG'GwC_CG
Cuts at: 3513 3513
Size: 5318
SacI G_AGCT'C
Cuts at: 2332 2332
Size: 5318
SalI G'TCGA_C
Cuts at: 2355 2355
Size: 5318
SapI GCTCTTCn'nnn_
Cuts at: 211 3341 3551 211
Size: 3130 210 1978
Fragments arranged by size:
3130 1978 210
SbfI CC_TGCA'GG
Cuts at: 2365 2365
Size: 5318
ScaI AGT'ACT
Cuts at: 4835 4835
Size: 5318
SfcI C'TryA_G
Cuts at: 33 526 2000 2361 3046 4039 4230 33
Size: 493 1474 361 685 993 191 1121
Fragments arranged by size:
1474 1121 993 685 493 361 191
SfoI GGC'GCC
Cuts at: 2750 2998 2750
Size: 248 5070
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5318
SmaI CCC'GGG
Cuts at: 2340 2340
Size: 5318
SmlI C'TyrA_G
Cuts at: 635 1564 2122 2307 2473 3388 3880 4142 635
Size: 929 558 185 166 915 492 262 1811
Fragments arranged by size:
1811 929 915 558 492 262 185 166
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5318
SphI G_CATG'C
Cuts at: 1923 2371 2871 1923
Size: 448 500 4370
Fragments arranged by size:
4370 500 448
StuI AGG'CCT
Cuts at: 1990 1990
Size: 5318
StyI C'CwwG_G
Cuts at: 107 113 237 1169 1962 2452 2806 107
Size: 6 124 932 793 490 354 2619
Fragments arranged by size:
2619 932 793 490 354 124 6
SwaI ATTT'AAAT
Cuts at: 1181 1181
Size: 5318
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 912 1243 1247 2257 2901 3034 3493 3702 912
Size: 331 4 1010 644 133 459 209 2528
Fragments arranged by size:
2528 1010 644 459 331 209 133 4
TatI w'GTAC_w
Cuts at: 824 2227 4833 824
Size: 1403 2606 1309
Fragments arranged by size:
2606 1403 1309
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4835 4867 4835
Size: 32 5286
Tth111I GACn'n_nGTC
Cuts at: 1651 3115 1651
Size: 1464 3854
XbaI T'CTAG_A
Cuts at: 1431 2349 1431
Size: 918 4400
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2813 2813
Size: 5318
XmaI C'CCGG_G
Cuts at: 2338 2338
Size: 5318
ZraI GAC'GTC
Cuts at: 2137 2137
Size: 5318
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AloI AscI AvrII BglI
BmgBI BmtI BsaXI BsgI BsiWI BssHII BstEII BstZ17I
DraIII FseI FspAI HindIII HpaI NheI NotI NruI
PacI PmlI PpuMI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI XhoI XmnI