Restriction map for
pF1KB5736
(Circular) (Six-base) MAPSORT of: pF1KB5736.seq Check: 6871 from: 1 to: 5321
pF1KB5736 5321 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1053 1053
Size: 5321
AatII G_ACGT'C
Cuts at: 859 859
Size: 5321
AccI GT'mk_AC
Cuts at: 1888 2359 1888
Size: 471 4850
Acc65I G'GTAC_C
Cuts at: 2337 2337
Size: 5321
AclI AA'CG_TT
Cuts at: 5161 5161
Size: 5321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 849 1682 3146 3578 4325 4593 849
Size: 833 1464 432 747 268 1577
Fragments arranged by size:
1577 1464 833 747 432 268
AflII C'TTAA_G
Cuts at: 805 805
Size: 5321
AflIII A'CryG_T
Cuts at: 2673 3777 4754 2673
Size: 1104 977 3240
Fragments arranged by size:
3240 1104 977
AgeI A'CCGG_T
Cuts at: 3709 3709
Size: 5321
AhdI GACnn_n'nnGTC
Cuts at: 1420 1579 1420
Size: 159 5162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 806 806
Size: 5321
AlwNI CAG_nnn'CTG
Cuts at: 1411 2626 4193 1411
Size: 1215 1567 2539
Fragments arranged by size:
2539 1567 1215
ApaI G_GGCC'C
Cuts at: 1941 2041 2211 1941
Size: 100 170 5051
Fragments arranged by size:
5051 170 100
ApaLI G'TGCA_C
Cuts at: 1129 4091 1129
Size: 2962 2359
ApoI r'AATT_y
Cuts at: 317 2325 4674 4930 317
Size: 2008 2349 256 708
Fragments arranged by size:
2349 2008 708 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5321
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
AvaI C'yCGr_G
Cuts at: 397 425 472 1927 1942 2341 397
Size: 28 47 1455 15 399 3377
Fragments arranged by size:
3377 1455 399 47 28 15
BamHI G'GATC_C
Cuts at: 1173 2346 1173
Size: 1173 4148
BanI G'GyrC_C
Cuts at: 262 438 1270 2068 2337 2751 2999 3034 262
Size: 176 832 798 269 414 248 35 2549
Fragments arranged by size:
2549 832 798 414 269 248 176 35
BanII G_rGCy'C
Cuts at: 691 1217 1474 1941 1979 2041 2088 2099 2211
Size: 526 257 467 38 62 47 11 112
Cuts at: 2211 2233 2335 3365 691
Size: 22 102 1030 2647
Fragments arranged by size:
2647 1030 526 467 257 112 102 62
47 38 22 11
BbeI G_GCGC'C
Cuts at: 266 442 2755 3003 266
Size: 176 2313 248 2584
Fragments arranged by size:
2584 2313 248 176
BbsI GAAGACnn'nnnn_
Cuts at: 1611 1611
Size: 5321
BbvCI CC'TCA_GC
Cuts at: 1117 1117
Size: 5321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 207 241 207
Size: 34 5287
BciVI GTATCCnnnnn_n'
Cuts at: 375 673 1701 3219 3980 5321 375
Size: 298 1028 1518 761 1341 375
Fragments arranged by size:
1518 1341 1028 761 375 298
BclI T'GATC_A
Cuts at: 2841 2841
Size: 5321
BglI GCCn_nnn'nGGC
Cuts at: 1991 1991
Size: 5321
BglII A'GATC_T
Cuts at: 2836 4873 2836
Size: 2037 3284
BlpI GC'TnA_GC
Cuts at: 2433 2433
Size: 5321
Bme1580I G_kGCm'C
Cuts at: 1133 1941 2041 2073 2211 2240 2946 3039 4095
Size: 808 100 32 138 29 706 93 1056
Cuts at: 4095 1133
Size: 2359
Fragments arranged by size:
2359 1056 808 706 138 100 93 32
29
BmrI ACTGGGnnnn_n'
Cuts at: 134 2072 2533 2614 2949 5093 134
Size: 1938 461 81 335 2144 362
Fragments arranged by size:
2144 1938 461 362 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 708 3688 708
Size: 2980 2341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 428 568 682 736 868 1388 1671 1965
Size: 33 140 114 54 132 520 283 294
Cuts at: 1965 2162 2171 2198 395
Size: 197 9 27 3518
Fragments arranged by size:
3518 520 294 283 197 140 132 114
54 33 27 9
Bpu10I CC'TnA_GC
Cuts at: 1117 1517 1117
Size: 400 4921
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 483 639 1426 2497 3376 3868 4166 483
Size: 156 787 1071 879 492 298 1638
Fragments arranged by size:
1638 1071 879 787 492 298 156
BsaI GGTCTCn'nnnn_
Cuts at: 1360 1619 2216 5025 1360
Size: 259 597 2809 1656
Fragments arranged by size:
2809 1656 597 259
BsaAI yAC'GTr
Cuts at: 1503 2674 1503
Size: 1171 4150
BsaBI GATnn'nnATC
Cuts at: 6 1450 2835 4872 6
Size: 1444 1385 2037 455
Fragments arranged by size:
2037 1444 1385 455
BsaHI Gr'CG_yC
Cuts at: 263 439 856 2752 3000 3702 5198 263
Size: 176 417 1896 248 702 1496 386
Fragments arranged by size:
1896 1496 702 417 386 248 176
BsaWI w'CCGG_w
Cuts at: 2510 3031 3709 3983 4130 4541 2510
Size: 521 678 274 147 411 3290
Fragments arranged by size:
3290 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 673 703 841 871 982 1012 1669 1699 1701
Size: 30 138 30 111 30 657 30 2
Cuts at: 1701 1731 673
Size: 30 4263
Fragments arranged by size:
4263 657 138 111 30 30 30 30
30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 681 760 1172 1287 1709 1945 2033 681
Size: 79 412 115 422 236 88 3969
Fragments arranged by size:
3969 422 412 236 115 88 79
BseYI C'CCAG_C
Cuts at: 628 1169 1278 2290 2746 3504 4081 4577 628
Size: 541 109 1012 456 758 577 496 1372
Fragments arranged by size:
1372 1012 758 577 541 496 456 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2215 2215
Size: 5321
BsiEI CG_ry'CG
Cuts at: 104 1028 2863 2909 4117 104
Size: 924 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 924 46
BsiHKAI G_wGCw'C
Cuts at: 691 1133 1309 2335 3113 3303 4095 691
Size: 442 176 1026 778 190 792 1917
Fragments arranged by size:
1917 1026 792 778 442 190 176
BsmBI CGTCTCn'nnnn_
Cuts at: 139 971 139
Size: 832 4489
Bsp1286I G_dGCh'C
Cuts at: 691 1133 1217 1309 1474 1941 1979 2041 2073
Size: 442 84 92 165 467 38 62 32
Cuts at: 2073 2088 2099 2211 2233 2240 2335 2946 3039
Size: 15 11 112 22 7 95 611 93
Cuts at: 3039 3113 3303 3365 4095 691
Size: 74 190 62 730 1917
Fragments arranged by size:
1917 730 611 467 442 190 165 112
95 93 92 84 74 62 62 38
32 22 15 11 7
BspEI T'CCGG_A
Cuts at: 4541 4541
Size: 5321
BspHI T'CATG_A
Cuts at: 701 1004 2156 4497 701
Size: 303 1152 2341 1525
Fragments arranged by size:
2341 1525 1152 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 752 1053 2371 2585 2887 3268 752
Size: 301 1318 214 302 381 2805
Fragments arranged by size:
2805 1318 381 302 301 214
BsrBI CCG'CTC
Cuts at: 50 594 972 1521 1935 3613 3667 5151 5316
Size: 544 378 549 414 1678 54 1484 165
Cuts at: 5316 50
Size: 55
Fragments arranged by size:
1678 1484 549 544 414 378 165 55
54
BsrDI GCAATG_nn'
Cuts at: 972 3233 972
Size: 2261 3060
BsrFI r'CCGG_y
Cuts at: 815 3319 3500 3709 815
Size: 2504 181 209 2427
Fragments arranged by size:
2504 2427 209 181
BssSI C'ACGA_G
Cuts at: 3592 3950 3592
Size: 358 4963
BstAPI GCAn_nnn'nTGC
Cuts at: 2698 2698
Size: 5321
BstBI TT'CG_AA
Cuts at: 3682 3682
Size: 5321
BstEII G'GTnAC_C
Cuts at: 1383 1383
Size: 5321
BstXI CCAn_nnnn'nTGG
Cuts at: 643 714 1395 2107 2151 643
Size: 71 681 712 44 3813
Fragments arranged by size:
3813 712 681 71 44
BstYI r'GATC_y
Cuts at: 1 197 1173 2346 2813 2836 3171 3417 4429
Size: 196 976 1173 467 23 335 246 1012
Cuts at: 4429 4515 4527 4873 1
Size: 86 12 346 449
Fragments arranged by size:
1173 1012 976 467 449 346 335 246
196 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1184 1184
Size: 5321
BtgI C'CryG_G
Cuts at: 107 257 776 1256 1394 2279 107
Size: 150 519 480 138 885 3149
Fragments arranged by size:
3149 885 519 480 150 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2045 3271 3452 3615 2045
Size: 1226 181 163 3751
Fragments arranged by size:
3751 1226 181 163
BtsI GCAGTG_nn'
Cuts at: 621 795 1612 1851 1887 1965 1971 2198 2564
Size: 174 817 239 36 78 6 227 366
Cuts at: 2564 621
Size: 3378
Fragments arranged by size:
3378 817 366 239 227 174 78 36
6
DraI TTT'AAA
Cuts at: 2320 2320
Size: 5321
DraIII CAC_nnn'GTG
Cuts at: 1897 1897
Size: 5321
DrdI GACnn_nn'nnGTC
Cuts at: 3027 3885 3027
Size: 858 4463
EaeI y'GGCC_r
Cuts at: 674 957 1025 1160 2276 2906 3080 3471 3498
Size: 283 68 135 1116 630 174 391 27
Cuts at: 3498 4843 674
Size: 1345 1152
Fragments arranged by size:
1345 1152 1116 630 391 283 174 135
68 27
EagI C'GGCC_G
Cuts at: 1025 2906 1025
Size: 1881 3440
EarI CTCTTCn'nnn_
Cuts at: 170 3344 3554 170
Size: 3174 210 1937
Fragments arranged by size:
3174 1937 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3529 3839 3985 5131 3529
Size: 310 146 1146 3719
Fragments arranged by size:
3719 1146 310 146
EcoICRI GAG'CTC
Cuts at: 689 2333 689
Size: 1644 3677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 428 568 682 736 849 868 1388 1671
Size: 33 140 114 54 113 19 520 283
Cuts at: 1671 1682 1965 2162 2171 2198 3146 3578 4325
Size: 11 283 197 9 27 948 432 747
Cuts at: 4325 4593 395
Size: 268 1123
Fragments arranged by size:
1123 948 747 520 432 283 283 268
197 140 114 113 54 33 27 19
11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 169 169
Size: 5321
EcoO109I rG'GnC_Cy
Cuts at: 1938 2037 2038 2207 2208 2460 1938
Size: 99 1 169 1 252 4799
Fragments arranged by size:
4799 252 169 99 1 1
EcoRI G'AATT_C
Cuts at: 2325 2325
Size: 5321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 951 1611 3340 951
Size: 660 1729 2932
Fragments arranged by size:
2932 1729 660
FspI TGC'GCA
Cuts at: 3102 3102
Size: 5321
HaeII r_GCGC'y
Cuts at: 266 442 629 669 813 2755 3003 4025 5004
Size: 176 187 40 144 1942 248 1022 979
Cuts at: 5004 266
Size: 583
Fragments arranged by size:
1942 1022 979 583 248 187 176 144
40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 393 425 711 743 841 873 1363 1395 1406
Size: 32 286 32 98 32 490 32 11
Cuts at: 1406 1438 1565 1597 1807 1839 1936 1968 2215
Size: 32 127 32 210 32 97 32 247
Cuts at: 2215 2247 3163 3193 3195 3225 393
Size: 32 916 30 2 30 2489
Fragments arranged by size:
2489 916 490 286 247 210 127 98
97 32 32 32 32 32 32 32
32 32 30 30 11 2
HincII GTy'rAC
Cuts at: 1485 1584 1659 1889 2360 1485
Size: 99 75 230 471 4446
Fragments arranged by size:
4446 471 230 99 75
HindIII A'AGCT_T
Cuts at: 2265 2265
Size: 5321
KasI G'GCGC_C
Cuts at: 262 438 2751 2999 262
Size: 176 2313 248 2584
Fragments arranged by size:
2584 2313 248 176
KpnI G_GTAC'C
Cuts at: 2341 2341
Size: 5321
MfeI C'AATT_G
Cuts at: 1226 1226
Size: 5321
MluI A'CGCG_T
Cuts at: 4754 4754
Size: 5321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1596 3992 4176 1596
Size: 2396 184 2741
Fragments arranged by size:
2741 2396 184
MscI TGG'CCA
Cuts at: 959 1162 2278 3082 959
Size: 203 1116 804 3198
Fragments arranged by size:
3198 1116 804 203
MslI CAynn'nnrTG
Cuts at: 167 521 2105 2149 2278 3310 3437 167
Size: 354 1584 44 129 1032 127 2051
Fragments arranged by size:
2051 1584 1032 354 129 127 44
MspA1I CmG'CkG
Cuts at: 252 259 485 1258 1547 2432 2746 3106 4119
Size: 7 226 773 289 885 314 360 1013
Cuts at: 4119 4364 252
Size: 245 1209
Fragments arranged by size:
1209 1013 885 773 360 314 289 245
226 7
NaeI GCC'GGC
Cuts at: 3502 3502
Size: 5321
NarI GG'CG_CC
Cuts at: 263 439 2752 3000 263
Size: 176 2313 248 2584
Fragments arranged by size:
2584 2313 248 176
NcoI C'CATG_G
Cuts at: 107 1394 2279 107
Size: 1287 885 3149
Fragments arranged by size:
3149 1287 885
NgoMIV G'CCGG_C
Cuts at: 3500 3500
Size: 5321
NspI r_CATG'y
Cuts at: 1056 1437 2374 2874 3781 1056
Size: 381 937 500 907 2596
Fragments arranged by size:
2596 937 907 500 381
PasI CC'CwG_GG
Cuts at: 2092 2092
Size: 5321
PciI A'CATG_T
Cuts at: 3777 3777
Size: 5321
PflMI CCAn_nnn'nTGG
Cuts at: 1169 1411 1503 1739 1169
Size: 242 92 236 4751
Fragments arranged by size:
4751 242 236 92
PfoI T'CCnGG_A
Cuts at: 1424 1424
Size: 5321
PmeI GTTT'AAAC
Cuts at: 2320 2320
Size: 5321
PmlI CAC'GTG
Cuts at: 1503 1503
Size: 5321
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 4460
Size: 32 5289
PspOMI G'GGCC_C
Cuts at: 1937 2037 2207 1937
Size: 100 170 5051
Fragments arranged by size:
5051 170 100
PstI C_TGCA'G
Cuts at: 251 2368 3053 251
Size: 2117 685 2519
Fragments arranged by size:
2519 2117 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5321
PvuII CAG'CTG
Cuts at: 2746 3106 2746
Size: 360 4961
RsrII CG'GwC_CG
Cuts at: 3516 3516
Size: 5321
SacI G_AGCT'C
Cuts at: 691 2335 691
Size: 1644 3677
SacII CC_GC'GG
Cuts at: 260 1259 260
Size: 999 4322
SalI G'TCGA_C
Cuts at: 1887 2358 1887
Size: 471 4850
SapI GCTCTTCn'nnn_
Cuts at: 3344 3554 3344
Size: 210 5111
SbfI CC_TGCA'GG
Cuts at: 2368 2368
Size: 5321
ScaI AGT'ACT
Cuts at: 271 4838 271
Size: 4567 754
SexAI A'CCwGG_T
Cuts at: 188 188
Size: 5321
SfcI C'TryA_G
Cuts at: 33 247 1788 2364 3049 4042 4233 33
Size: 214 1541 576 685 993 191 1121
Fragments arranged by size:
1541 1121 993 685 576 214 191
SfoI GGC'GCC
Cuts at: 264 440 2753 3001 264
Size: 176 2313 248 2584
Fragments arranged by size:
2584 2313 248 176
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5321
SgrAI Cr'CCGG_yG
Cuts at: 815 815
Size: 5321
SmaI CCC'GGG
Cuts at: 2343 2343
Size: 5321
SmlI C'TyrA_G
Cuts at: 498 654 805 1441 2476 3391 3883 4145 498
Size: 156 151 636 1035 915 492 262 1674
Fragments arranged by size:
1674 1035 915 636 492 262 156 151
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5321
SphI G_CATG'C
Cuts at: 2374 2874 2374
Size: 500 4821
StuI AGG'CCT
Cuts at: 504 1189 1565 504
Size: 685 376 4260
Fragments arranged by size:
4260 685 376
StyI C'CwwG_G
Cuts at: 107 1388 1394 2279 2455 2809 107
Size: 1281 6 885 176 354 2619
Fragments arranged by size:
2619 1281 885 354 176 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1233 2105 2904 3037 3496 3705 1233
Size: 872 799 133 459 209 2849
Fragments arranged by size:
2849 872 799 459 209 133
TatI w'GTAC_w
Cuts at: 269 929 4836 269
Size: 660 3907 754
Fragments arranged by size:
3907 754 660
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1420 1452 2165 2197 4838 4870 1420
Size: 32 713 32 2641 32 1871
Fragments arranged by size:
2641 1871 713 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3118 3118
Size: 5321
XbaI T'CTAG_A
Cuts at: 2352 2352
Size: 5321
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 643 2816 643
Size: 2173 3148
XmaI C'CCGG_G
Cuts at: 2341 2341
Size: 5321
ZraI GAC'GTC
Cuts at: 857 857
Size: 5321
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AleI AloI AscI AvrII BaeI BfrBI BmgBI
BmtI BsiWI BsmI BsrGI BssHII BstZ17I ClaI CspCI
EcoRV FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PpuMI PshAI PsiI PspXI PsrI SanDI
SfiI SnaBI SrfI SspI SwaI XhoI XmnI