Restriction map for
pF1KB5737
(Circular) (Six-base) MAPSORT of: pF1KB5737.seq Check: 3890 from: 1 to: 5498
pF1KB5737 5498 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 689 1322 2536 689
Size: 633 1214 3651
Fragments arranged by size:
3651 1214 633
Acc65I G'GTAC_C
Cuts at: 1438 2514 1438
Size: 1076 4422
AclI AA'CG_TT
Cuts at: 5338 5338
Size: 5498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 491 815 1111 1606 1615 2163 3323 3755 4502
Size: 324 296 495 9 548 1160 432 747
Cuts at: 4502 4770 491
Size: 268 1219
Fragments arranged by size:
1219 1160 747 548 495 432 324 296
268 9
AfeI AGC'GCT
Cuts at: 1357 1357
Size: 5498
AflIII A'CryG_T
Cuts at: 2850 3954 4931 2850
Size: 1104 977 3417
Fragments arranged by size:
3417 1104 977
AgeI A'CCGG_T
Cuts at: 3886 3886
Size: 5498
AleI CACnn'nnGTG
Cuts at: 997 1723 997
Size: 726 4772
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1094 1126 1094
Size: 32 5466
AlwNI CAG_nnn'CTG
Cuts at: 380 1416 1584 1632 1774 2289 2803 4370 380
Size: 1036 168 48 142 515 514 1567 1508
Fragments arranged by size:
1567 1508 1036 515 514 168 142 48
ApaI G_GGCC'C
Cuts at: 181 1311 2126 181
Size: 1130 815 3553
Fragments arranged by size:
3553 1130 815
ApaLI G'TGCA_C
Cuts at: 1726 4268 1726
Size: 2542 2956
ApoI r'AATT_y
Cuts at: 2502 4851 5107 2502
Size: 2349 256 2893
Fragments arranged by size:
2893 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5498
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
AvaI C'yCGr_G
Cuts at: 156 217 1298 1304 2518 156
Size: 61 1081 6 1214 3136
Fragments arranged by size:
3136 1214 1081 61 6
BamHI G'GATC_C
Cuts at: 827 2523 827
Size: 1696 3802
BanI G'GyrC_C
Cuts at: 465 663 1120 1209 1438 2109 2171 2514 2928
Size: 198 457 89 229 671 62 343 414
Cuts at: 2928 3176 3211 465
Size: 248 35 2752
Fragments arranged by size:
2752 671 457 414 343 248 229 198
89 62 35
BanII G_rGCy'C
Cuts at: 181 285 1099 1305 1311 2126 2512 3542 181
Size: 104 814 206 6 815 386 1030 2137
Fragments arranged by size:
2137 1030 815 814 386 206 104 6
BbeI G_GCGC'C
Cuts at: 469 1124 2175 2932 3180 469
Size: 655 1051 757 248 2787
Fragments arranged by size:
2787 1051 757 655 248
BbsI GAAGACnn'nnnn_
Cuts at: 1177 1524 1177
Size: 347 5151
BciVI GTATCCnnnnn_n'
Cuts at: 3396 4157 5498 3396
Size: 761 1341 3396
Fragments arranged by size:
3396 1341 761
BclI T'GATC_A
Cuts at: 3018 3018
Size: 5498
BglI GCCn_nnn'nGGC
Cuts at: 1160 1201 2159 2364 2484 1160
Size: 41 958 205 120 4174
Fragments arranged by size:
4174 958 205 120 41
BglII A'GATC_T
Cuts at: 3013 5050 3013
Size: 2037 3461
BlpI GC'TnA_GC
Cuts at: 2610 2610
Size: 5498
Bme1580I G_kGCm'C
Cuts at: 138 181 1214 1311 1730 2049 2126 3123 3216
Size: 43 1033 97 419 319 77 997 93
Cuts at: 3216 4272 138
Size: 1056 1364
Fragments arranged by size:
1364 1056 1033 997 419 319 97 93
77 43
BmgBI CAC'GTC
Cuts at: 678 1038 1530 678
Size: 360 492 4646
Fragments arranged by size:
4646 492 360
BmrI ACTGGGnnnn_n'
Cuts at: 826 1599 2710 2791 3126 5270 826
Size: 773 1111 81 335 2144 1054
Fragments arranged by size:
2144 1111 1054 773 335 81
BmtI G_CTAG'C
Cuts at: 245 733 245
Size: 488 5010
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3865 3865
Size: 5498
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 293 722 1096 1882 213
Size: 80 429 374 786 3829
Fragments arranged by size:
3829 786 429 374 80
Bpu10I CC'TnA_GC
Cuts at: 1205 1205
Size: 5498
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 402 2674 3553 4045 4343 402
Size: 2272 879 492 298 1557
Fragments arranged by size:
2272 1557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1893 1937 2431 2440 5202 1893
Size: 44 494 9 2762 2189
Fragments arranged by size:
2762 2189 494 44 9
BsaAI yAC'GTr
Cuts at: 2851 2851
Size: 5498
BsaBI GATnn'nnATC
Cuts at: 6 3012 5049 6
Size: 3006 2037 455
Fragments arranged by size:
3006 2037 455
BsaHI Gr'CG_yC
Cuts at: 466 495 1121 1465 2172 2402 2929 3177 3879
Size: 29 626 344 707 230 527 248 702
Cuts at: 3879 5375 466
Size: 1496 589
Fragments arranged by size:
1496 707 702 626 589 527 344 248
230 29
BsaWI w'CCGG_w
Cuts at: 2687 3208 3886 4160 4307 4718 2687
Size: 521 678 274 147 411 3467
Fragments arranged by size:
3467 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 483 513 695 725 1094 1124 1957 1987 483
Size: 30 182 30 369 30 833 30 3994
Fragments arranged by size:
3994 833 369 182 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 504 1405 1408 2446 2449 499
Size: 5 901 3 1038 3 3548
Fragments arranged by size:
3548 1038 901 5 3 3
BseYI C'CCAG_C
Cuts at: 1625 2450 2923 3681 4258 4754 1625
Size: 825 473 758 577 496 2369
Fragments arranged by size:
2369 825 758 577 496 473
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 679 1681 667
Size: 12 1002 4484
Fragments arranged by size:
4484 1002 12
BsiEI CG_ry'CG
Cuts at: 104 169 232 3040 3086 4294 104
Size: 65 63 2808 46 1208 1308
Fragments arranged by size:
2808 1308 1208 65 63 46
BsiHKAI G_wGCw'C
Cuts at: 285 1500 1730 1868 2512 3290 3480 4272 285
Size: 1215 230 138 644 778 190 792 1511
Fragments arranged by size:
1511 1215 792 778 644 230 190 138
BsmI GAATG_Cn'
Cuts at: 579 752 1052 579
Size: 173 300 5025
Fragments arranged by size:
5025 300 173
BsmBI CGTCTCn'nnnn_
Cuts at: 486 486
Size: 5498
Bsp1286I G_dGCh'C
Cuts at: 138 181 285 1099 1214 1305 1311 1500 1730
Size: 43 104 814 115 91 6 189 230
Cuts at: 1730 1868 2049 2126 2512 3123 3216 3290 3480
Size: 138 181 77 386 611 93 74 190
Cuts at: 3480 3542 4272 138
Size: 62 730 1364
Fragments arranged by size:
1364 814 730 611 386 230 190 189
181 138 115 104 93 91 77 74
62 43 6
BspEI T'CCGG_A
Cuts at: 4718 4718
Size: 5498
BspHI T'CATG_A
Cuts at: 560 1827 4674 560
Size: 1267 2847 1384
Fragments arranged by size:
2847 1384 1267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 701 741 2548 2762 3064 3445 701
Size: 40 1807 214 302 381 2754
Fragments arranged by size:
2754 1807 381 302 214 40
BsrBI CCG'CTC
Cuts at: 50 504 528 1980 3790 3844 5328 5493 50
Size: 454 24 1452 1810 54 1484 165 55
Fragments arranged by size:
1810 1484 1452 454 165 55 54 24
BsrDI GCAATG_nn'
Cuts at: 1689 3410 1689
Size: 1721 3777
BsrFI r'CCGG_y
Cuts at: 1228 1523 3496 3677 3886 1228
Size: 295 1973 181 209 2840
Fragments arranged by size:
2840 1973 295 209 181
BssSI C'ACGA_G
Cuts at: 622 3769 4127 622
Size: 3147 358 1993
Fragments arranged by size:
3147 1993 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1774 2875 1774
Size: 1101 4397
BstBI TT'CG_AA
Cuts at: 3859 3859
Size: 5498
BstEII G'GTnAC_C
Cuts at: 2067 2067
Size: 5498
BstXI CCAn_nnnn'nTGG
Cuts at: 1191 1191
Size: 5498
BstYI r'GATC_y
Cuts at: 1 827 1790 2523 2990 3013 3348 3594 4606
Size: 826 963 733 467 23 335 246 1012
Cuts at: 4606 4692 4704 5050 1
Size: 86 12 346 449
Fragments arranged by size:
1012 963 826 733 467 449 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2059 2138 2059
Size: 79 5419
BtgI C'CryG_G
Cuts at: 107 707 1196 1751 2087 2361 107
Size: 600 489 555 336 274 3244
Fragments arranged by size:
3244 600 555 489 336 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 534 1237 1653 2061 3448 3629 3792 534
Size: 703 416 408 1387 181 163 2240
Fragments arranged by size:
2240 1387 703 416 408 181 163
BtsI GCAGTG_nn'
Cuts at: 899 1141 2741 899
Size: 242 1600 3656
Fragments arranged by size:
3656 1600 242
ClaI AT'CG_AT
Cuts at: 1328 1328
Size: 5498
DraI TTT'AAA
Cuts at: 2497 2497
Size: 5498
DrdI GACnn_nn'nnGTC
Cuts at: 1528 3204 4062 1528
Size: 1676 858 2964
Fragments arranged by size:
2964 1676 858
EaeI y'GGCC_r
Cuts at: 110 166 229 632 902 929 1181 1193 1226
Size: 56 63 403 270 27 252 12 33
Cuts at: 1226 2228 3083 3257 3648 3675 5020 110
Size: 1002 855 174 391 27 1345 588
Fragments arranged by size:
1345 1002 855 588 403 391 270 252
174 63 56 33 27 27 12
EagI C'GGCC_G
Cuts at: 166 229 3083 166
Size: 63 2854 2581
Fragments arranged by size:
2854 2581 63
EarI CTCTTCn'nnn_
Cuts at: 467 476 770 1087 1582 1960 2438 2468 3521
Size: 9 294 317 495 378 478 30 1053
Cuts at: 3521 3731 467
Size: 210 2234
Fragments arranged by size:
2234 1053 495 478 378 317 294 210
30 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 3706 4016 4162 5308 130
Size: 3576 310 146 1146 320
Fragments arranged by size:
3576 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 283 2510 283
Size: 2227 3271
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 293 491 722 815 1096 1111 1606 1615
Size: 80 198 231 93 281 15 495 9
Cuts at: 1615 1882 2163 3323 3755 4502 4770 213
Size: 267 281 1160 432 747 268 941
Fragments arranged by size:
1160 941 747 495 432 281 281 268
267 231 198 93 80 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 265 1288 1798 2062 265
Size: 1023 510 264 3701
Fragments arranged by size:
3701 1023 510 264
EcoO109I rG'GnC_Cy
Cuts at: 160 177 258 1128 1308 2151 2637 160
Size: 17 81 870 180 843 486 3021
Fragments arranged by size:
3021 870 843 486 180 81 17
EcoRI G'AATT_C
Cuts at: 2502 2502
Size: 5498
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 799 3517 799
Size: 2718 2780
FseI GG_CCGG'CC
Cuts at: 1232 1232
Size: 5498
FspI TGC'GCA
Cuts at: 535 3279 535
Size: 2744 2754
HaeII r_GCGC'y
Cuts at: 469 1124 1268 1359 2175 2290 2932 3180 4202
Size: 655 144 91 816 115 642 248 1022
Cuts at: 4202 5181 469
Size: 979 786
Fragments arranged by size:
1022 979 816 786 655 642 248 144
115 91
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3340 3370 3372 3402 3340
Size: 30 2 30 5436
Fragments arranged by size:
5436 30 30 2
HincII GTy'rAC
Cuts at: 2537 2537
Size: 5498
HindIII A'AGCT_T
Cuts at: 781 781
Size: 5498
KasI G'GCGC_C
Cuts at: 465 1120 2171 2928 3176 465
Size: 655 1051 757 248 2787
Fragments arranged by size:
2787 1051 757 655 248
KpnI G_GTAC'C
Cuts at: 1442 2518 1442
Size: 1076 4422
MfeI C'AATT_G
Cuts at: 298 298
Size: 5498
MluI A'CGCG_T
Cuts at: 4931 4931
Size: 5498
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1406 4169 4353 1406
Size: 2763 184 2551
Fragments arranged by size:
2763 2551 184
MscI TGG'CCA
Cuts at: 634 904 1183 1195 2230 3259 634
Size: 270 279 12 1035 1029 2873
Fragments arranged by size:
2873 1035 1029 279 270 12
MslI CAynn'nnrTG
Cuts at: 112 997 1366 1723 3487 3614 112
Size: 885 369 357 1764 127 1996
Fragments arranged by size:
1996 1764 885 369 357 127
MspA1I CmG'CkG
Cuts at: 171 590 793 1971 2609 2923 3283 4296 4541
Size: 419 203 1178 638 314 360 1013 245
Cuts at: 4541 171
Size: 1128
Fragments arranged by size:
1178 1128 1013 638 419 360 314 245
203
NaeI GCC'GGC
Cuts at: 1230 3679 1230
Size: 2449 3049
NarI GG'CG_CC
Cuts at: 466 1121 2172 2929 3177 466
Size: 655 1051 757 248 2787
Fragments arranged by size:
2787 1051 757 655 248
NcoI C'CATG_G
Cuts at: 107 2087 2361 107
Size: 1980 274 3244
Fragments arranged by size:
3244 1980 274
NgoMIV G'CCGG_C
Cuts at: 1228 3677 1228
Size: 2449 3049
NheI G'CTAG_C
Cuts at: 241 729 241
Size: 488 5010
NspI r_CATG'y
Cuts at: 2551 3051 3958 2551
Size: 500 907 4091
Fragments arranged by size:
4091 907 500
PasI CC'CwG_GG
Cuts at: 1917 1995 1917
Size: 78 5420
PciI A'CATG_T
Cuts at: 3954 3954
Size: 5498
PflMI CCAn_nnn'nTGG
Cuts at: 911 1416 1632 1845 911
Size: 505 216 213 4564
Fragments arranged by size:
4564 505 216 213
PfoI T'CCnGG_A
Cuts at: 604 604
Size: 5498
PmeI GTTT'AAAC
Cuts at: 2497 2497
Size: 5498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1094 1126 4637 4669 1094
Size: 32 3511 32 1923
Fragments arranged by size:
3511 1923 32 32
PpuMI rG'GwC_Cy
Cuts at: 160 258 1128 160
Size: 98 870 4530
Fragments arranged by size:
4530 870 98
PspOMI G'GGCC_C
Cuts at: 177 1307 2122 177
Size: 1130 815 3553
Fragments arranged by size:
3553 1130 815
PstI C_TGCA'G
Cuts at: 290 1556 2195 2264 2545 3230 290
Size: 1266 639 69 281 685 2558
Fragments arranged by size:
2558 1266 685 639 281 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5498
PvuII CAG'CTG
Cuts at: 793 1971 2923 3283 793
Size: 1178 952 360 3008
Fragments arranged by size:
3008 1178 952 360
RsrII CG'GwC_CG
Cuts at: 3693 3693
Size: 5498
SacI G_AGCT'C
Cuts at: 285 2512 285
Size: 2227 3271
SalI G'TCGA_C
Cuts at: 2535 2535
Size: 5498
SapI GCTCTTCn'nnn_
Cuts at: 1087 1582 2468 3521 3731 1087
Size: 495 886 1053 210 2854
Fragments arranged by size:
2854 1053 886 495 210
SbfI CC_TGCA'GG
Cuts at: 2545 2545
Size: 5498
ScaI AGT'ACT
Cuts at: 5015 5015
Size: 5498
SfcI C'TryA_G
Cuts at: 33 286 1552 2191 2238 2260 2541 3226 4219
Size: 253 1266 639 47 22 281 685 993
Cuts at: 4219 4410 33
Size: 191 1121
Fragments arranged by size:
1266 1121 993 685 639 281 253 191
47 22
SfoI GGC'GCC
Cuts at: 467 1122 2173 2930 3178 467
Size: 655 1051 757 248 2787
Fragments arranged by size:
2787 1051 757 655 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5498
SmaI CCC'GGG
Cuts at: 158 219 1306 2520 158
Size: 61 1087 1214 3136
Fragments arranged by size:
3136 1214 1087 61
SmlI C'TyrA_G
Cuts at: 417 2653 3568 4060 4322 417
Size: 2236 915 492 262 1593
Fragments arranged by size:
2236 1593 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5498
SphI G_CATG'C
Cuts at: 2551 3051 2551
Size: 500 4998
SrfI GCCC'GGGC
Cuts at: 1306 1306
Size: 5498
SspI AAT'ATT
Cuts at: 762 762
Size: 5498
StuI AGG'CCT
Cuts at: 249 1259 2189 2236 249
Size: 1010 930 47 3511
Fragments arranged by size:
3511 1010 930 47
StyI C'CwwG_G
Cuts at: 107 635 818 1124 1184 1799 1844 1961 2087
Size: 528 183 306 60 615 45 117 126
Cuts at: 2087 2184 2231 2361 2489 2632 2986 107
Size: 97 47 130 128 143 354 2619
Fragments arranged by size:
2619 615 528 354 306 183 143 130
128 126 117 97 60 47 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2455 3081 3214 3673 3882 2455
Size: 626 133 459 209 4071
Fragments arranged by size:
4071 626 459 209 133
TatI w'GTAC_w
Cuts at: 872 5013 872
Size: 4141 1357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 483 515 695 727 5015 5047 483
Size: 32 180 32 4288 32 934
Fragments arranged by size:
4288 934 180 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 616 1450 1605 3295 616
Size: 834 155 1690 2819
Fragments arranged by size:
2819 1690 834 155
XbaI T'CTAG_A
Cuts at: 2529 2529
Size: 5498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 188 377 2993 188
Size: 189 2616 2693
Fragments arranged by size:
2693 2616 189
XmaI C'CCGG_G
Cuts at: 156 217 1304 2518 156
Size: 61 1087 1214 3136
Fragments arranged by size:
3136 1214 1087 61
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BsiWI BsrGI BssHII BstZ17I CspCI
DraIII EcoRV FspAI HpaI NdeI NotI NruI NsiI
PacI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SwaI XhoI XmnI ZraI