Restriction map for
pF1KB5738
(Circular) (Six-base) MAPSORT of: pF1KB5738.seq Check: 7782 from: 1 to: 5333
pF1KB5738 5333 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 556 2371 556
Size: 1815 3518
Acc65I G'GTAC_C
Cuts at: 1988 2142 2349 1988
Size: 154 207 4972
Fragments arranged by size:
4972 207 154
AclI AA'CG_TT
Cuts at: 5173 5173
Size: 5333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 1294 1586 3158 3590 4337 4605 347
Size: 947 292 1572 432 747 268 1075
Fragments arranged by size:
1572 1075 947 747 432 292 268
AflIII A'CryG_T
Cuts at: 711 2685 3789 4766 711
Size: 1974 1104 977 1278
Fragments arranged by size:
1974 1278 1104 977
AgeI A'CCGG_T
Cuts at: 3721 3721
Size: 5333
AhdI GACnn_n'nnGTC
Cuts at: 1167 1167
Size: 5333
AlwNI CAG_nnn'CTG
Cuts at: 1426 1472 2638 4205 1426
Size: 46 1166 1567 2554
Fragments arranged by size:
2554 1567 1166 46
ApaLI G'TGCA_C
Cuts at: 4103 4103
Size: 5333
ApoI r'AATT_y
Cuts at: 661 823 844 2155 2337 4686 4942 661
Size: 162 21 1311 182 2349 256 1052
Fragments arranged by size:
2349 1311 1052 256 182 162 21
AseI AT'TA_AT
Cuts at: 20 490 20
Size: 470 4863
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
AvaI C'yCGr_G
Cuts at: 992 1094 2353 992
Size: 102 1259 3972
Fragments arranged by size:
3972 1259 102
AvrII C'CTAG_G
Cuts at: 579 579
Size: 5333
BamHI G'GATC_C
Cuts at: 2358 2358
Size: 5333
BanI G'GyrC_C
Cuts at: 888 1988 2142 2232 2349 2763 3011 3046 888
Size: 1100 154 90 117 414 248 35 3175
Fragments arranged by size:
3175 1100 414 248 154 117 90 35
BanII G_rGCy'C
Cuts at: 2347 3377 2347
Size: 1030 4303
BbeI G_GCGC'C
Cuts at: 2767 3015 2767
Size: 248 5085
BbsI GAAGACnn'nnnn_
Cuts at: 1158 1415 1732 1158
Size: 257 317 4759
Fragments arranged by size:
4759 317 257
BciVI GTATCCnnnnn_n'
Cuts at: 528 3231 3992 5333 528
Size: 2703 761 1341 528
Fragments arranged by size:
2703 1341 761 528
BclI T'GATC_A
Cuts at: 2853 2853
Size: 5333
BfrBI ATG'CAT
Cuts at: 672 1377 672
Size: 705 4628
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5333
BglII A'GATC_T
Cuts at: 738 2848 4885 738
Size: 2110 2037 1186
Fragments arranged by size:
2110 2037 1186
BlpI GC'TnA_GC
Cuts at: 2445 2445
Size: 5333
Bme1580I G_kGCm'C
Cuts at: 2958 3051 4107 2958
Size: 93 1056 4184
Fragments arranged by size:
4184 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1441 2545 2626 2961 5105 1441
Size: 1104 81 335 2144 1669
Fragments arranged by size:
2144 1669 1104 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3700 3700
Size: 5333
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1178 1223 2020 2509 3388 3880 4178 1178
Size: 45 797 489 879 492 298 2333
Fragments arranged by size:
2333 879 797 492 489 298 45
BsaI GGTCTCn'nnnn_
Cuts at: 5037 5037
Size: 5333
BsaAI yAC'GTr
Cuts at: 1905 2686 1905
Size: 781 4552
BsaBI GATnn'nnATC
Cuts at: 6 2847 4884 6
Size: 2841 2037 455
Fragments arranged by size:
2841 2037 455
BsaHI Gr'CG_yC
Cuts at: 2764 3012 3714 5210 2764
Size: 248 702 1496 2887
Fragments arranged by size:
2887 1496 702 248
BsaWI w'CCGG_w
Cuts at: 891 2522 3043 3721 3995 4142 4553 891
Size: 1631 521 678 274 147 411 1671
Fragments arranged by size:
1671 1631 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 215 245 215
Size: 30 5303
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 236 1712 166
Size: 70 1476 3787
Fragments arranged by size:
3787 1476 70
BseYI C'CCAG_C
Cuts at: 2758 3516 4093 4589 2758
Size: 758 577 496 3502
Fragments arranged by size:
3502 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2875 2921 4129 104
Size: 2771 46 1208 1308
Fragments arranged by size:
2771 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2347 3125 3315 4107 2347
Size: 778 190 792 3573
Fragments arranged by size:
3573 792 778 190
BsmI GAATG_Cn'
Cuts at: 816 1338 816
Size: 522 4811
Bsp1286I G_dGCh'C
Cuts at: 2347 2958 3051 3125 3315 3377 4107 2347
Size: 611 93 74 190 62 730 3573
Fragments arranged by size:
3573 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4553 4553
Size: 5333
BspHI T'CATG_A
Cuts at: 525 1761 4509 525
Size: 1236 2748 1349
Fragments arranged by size:
2748 1349 1236
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2383 2597 2899 3280 2383
Size: 214 302 381 4436
Fragments arranged by size:
4436 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3625 3679 5163 5328 50
Size: 3575 54 1484 165 55
Fragments arranged by size:
3575 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3245 3245
Size: 5333
BsrFI r'CCGG_y
Cuts at: 3331 3512 3721 3331
Size: 181 209 4943
Fragments arranged by size:
4943 209 181
BsrGI T'GTAC_A
Cuts at: 1428 1512 1428
Size: 84 5249
BssSI C'ACGA_G
Cuts at: 3604 3962 3604
Size: 358 4975
BstAPI GCAn_nnn'nTGC
Cuts at: 2710 2710
Size: 5333
BstBI TT'CG_AA
Cuts at: 3694 3694
Size: 5333
BstEII G'GTnAC_C
Cuts at: 1287 1287
Size: 5333
BstXI CCAn_nnnn'nTGG
Cuts at: 1559 1559
Size: 5333
BstYI r'GATC_y
Cuts at: 1 403 738 2358 2825 2848 3183 3429 4441
Size: 402 335 1620 467 23 335 246 1012
Cuts at: 4441 4527 4539 4885 1
Size: 86 12 346 449
Fragments arranged by size:
1620 1012 467 449 402 346 335 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1283 1283
Size: 5333
BtgI C'CryG_G
Cuts at: 107 1851 2007 107
Size: 1744 156 3433
Fragments arranged by size:
3433 1744 156
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3283 3464 3627 3283
Size: 181 163 4989
Fragments arranged by size:
4989 181 163
BtsI GCAGTG_nn'
Cuts at: 2576 2576
Size: 5333
DraI TTT'AAA
Cuts at: 2332 2332
Size: 5333
DrdI GACnn_nn'nnGTC
Cuts at: 3039 3897 3039
Size: 858 4475
EaeI y'GGCC_r
Cuts at: 2918 3092 3483 3510 4855 2918
Size: 174 391 27 1345 3396
Fragments arranged by size:
3396 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2918 2918
Size: 5333
EarI CTCTTCn'nnn_
Cuts at: 155 833 1923 3356 3566 155
Size: 678 1090 1433 210 1922
Fragments arranged by size:
1922 1433 1090 678 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3541 3851 3997 5143 3541
Size: 310 146 1146 3731
Fragments arranged by size:
3731 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2345 2345
Size: 5333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 347 1294 1586 3158 3590 4337 4605 347
Size: 947 292 1572 432 747 268 1075
Fragments arranged by size:
1572 1075 947 747 432 292 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2263 2263
Size: 5333
EcoO109I rG'GnC_Cy
Cuts at: 1702 2472 1702
Size: 770 4563
EcoRI G'AATT_C
Cuts at: 661 2337 661
Size: 1676 3657
EcoRV GAT'ATC
Cuts at: 189 1848 189
Size: 1659 3674
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1203 1794 3352 1203
Size: 591 1558 3184
Fragments arranged by size:
3184 1558 591
FspI TGC'GCA
Cuts at: 3114 3114
Size: 5333
HaeII r_GCGC'y
Cuts at: 2767 3015 4037 5016 2767
Size: 248 1022 979 3084
Fragments arranged by size:
3084 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 388 420 1090 1122 1153 1185 1193 1225 1257
Size: 32 670 32 31 32 8 32 32
Cuts at: 1257 1289 1546 1578 3175 3205 3207 3237 388
Size: 32 257 32 1597 30 2 30 2484
Fragments arranged by size:
2484 1597 670 257 32 32 32 32
32 32 32 31 30 30 8 2
HincII GTy'rAC
Cuts at: 1172 1395 2372 1172
Size: 223 977 4133
Fragments arranged by size:
4133 977 223
KasI G'GCGC_C
Cuts at: 2763 3011 2763
Size: 248 5085
KpnI G_GTAC'C
Cuts at: 1992 2146 2353 1992
Size: 154 207 4972
Fragments arranged by size:
4972 207 154
MfeI C'AATT_G
Cuts at: 416 416
Size: 5333
MluI A'CGCG_T
Cuts at: 4766 4766
Size: 5333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4004 4188 4004
Size: 184 5149
MscI TGG'CCA
Cuts at: 3094 3094
Size: 5333
MslI CAynn'nnrTG
Cuts at: 509 3322 3449 509
Size: 2813 127 2393
Fragments arranged by size:
2813 2393 127
MspA1I CmG'CkG
Cuts at: 2313 2444 2758 3118 4131 4376 2313
Size: 131 314 360 1013 245 3270
Fragments arranged by size:
3270 1013 360 314 245 131
NaeI GCC'GGC
Cuts at: 3514 3514
Size: 5333
NarI GG'CG_CC
Cuts at: 2764 3012 2764
Size: 248 5085
NcoI C'CATG_G
Cuts at: 107 107
Size: 5333
NdeI CA'TA_TG
Cuts at: 393 770 2059 393
Size: 377 1289 3667
Fragments arranged by size:
3667 1289 377
NgoMIV G'CCGG_C
Cuts at: 3512 3512
Size: 5333
NsiI A_TGCA'T
Cuts at: 674 1379 674
Size: 705 4628
NspI r_CATG'y
Cuts at: 715 1519 2386 2886 3793 715
Size: 804 867 500 907 2255
Fragments arranged by size:
2255 907 867 804 500
PciI A'CATG_T
Cuts at: 711 3789 711
Size: 3078 2255
PflMI CCAn_nnn'nTGG
Cuts at: 865 865
Size: 5333
PfoI T'CCnGG_A
Cuts at: 1146 1211 1146
Size: 65 5268
PmeI GTTT'AAAC
Cuts at: 2332 2332
Size: 5333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 5301
PpuMI rG'GwC_Cy
Cuts at: 1702 1702
Size: 5333
PsiI TTA'TAA
Cuts at: 1258 1870 2024 1258
Size: 612 154 4567
Fragments arranged by size:
4567 612 154
PstI C_TGCA'G
Cuts at: 246 2380 3065 246
Size: 2134 685 2514
Fragments arranged by size:
2514 2134 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5333
PvuII CAG'CTG
Cuts at: 2313 2758 3118 2313
Size: 445 360 4528
Fragments arranged by size:
4528 445 360
RsrII CG'GwC_CG
Cuts at: 3528 3528
Size: 5333
SacI G_AGCT'C
Cuts at: 2347 2347
Size: 5333
SalI G'TCGA_C
Cuts at: 2370 2370
Size: 5333
SapI GCTCTTCn'nnn_
Cuts at: 1923 3356 3566 1923
Size: 1433 210 3690
Fragments arranged by size:
3690 1433 210
SbfI CC_TGCA'GG
Cuts at: 2380 2380
Size: 5333
ScaI AGT'ACT
Cuts at: 1681 4850 1681
Size: 3169 2164
SexAI A'CCwGG_T
Cuts at: 1536 1536
Size: 5333
SfcI C'TryA_G
Cuts at: 33 242 1644 2376 3061 4054 4245 33
Size: 209 1402 732 685 993 191 1121
Fragments arranged by size:
1402 1121 993 732 685 209 191
SfoI GGC'GCC
Cuts at: 2765 3013 2765
Size: 248 5085
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
SmaI CCC'GGG
Cuts at: 994 1096 2355 994
Size: 102 1259 3972
Fragments arranged by size:
3972 1259 102
SmlI C'TyrA_G
Cuts at: 1193 1238 2035 2488 3403 3895 4157 1193
Size: 45 797 453 915 492 262 2369
Fragments arranged by size:
2369 915 797 492 453 262 45
SnaBI TAC'GTA
Cuts at: 1905 1905
Size: 5333
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5333
SphI G_CATG'C
Cuts at: 2386 2886 2386
Size: 500 4833
StuI AGG'CCT
Cuts at: 568 568
Size: 5333
StyI C'CwwG_G
Cuts at: 107 531 579 884 2467 2821 107
Size: 424 48 305 1583 354 2619
Fragments arranged by size:
2619 1583 424 354 305 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 118 458 2916 3049 3508 3717 118
Size: 340 2458 133 459 209 1734
Fragments arranged by size:
2458 1734 459 340 209 133
TatI w'GTAC_w
Cuts at: 231 1428 1512 1679 4848 231
Size: 1197 84 167 3169 716
Fragments arranged by size:
3169 1197 716 167 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4850 4882 4850
Size: 32 5301
Tth111I GACn'n_nGTC
Cuts at: 3130 3130
Size: 5333
XbaI T'CTAG_A
Cuts at: 2364 2364
Size: 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2828 2828
Size: 5333
XmaI C'CCGG_G
Cuts at: 992 1094 2353 992
Size: 102 1259 3972
Fragments arranged by size:
3972 1259 102
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SnaBI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI BaeI BbvCI BcgI BmgBI BmtI BpmI Bpu10I
BsgI BsiWI BsmBI BssHII BstZ17I ClaI CspCI DraIII
FseI FspAI HindIII HpaI NheI NotI NruI PacI
PasI PmlI PshAI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SrfI SspI SwaI XhoI XmnI ZraI