Restriction map for
pF1KB5740
(Circular) (Six-base) MAPSORT of: pF1KB5740.seq Check: 3607 from: 1 to: 5333
pF1KB5740 5333 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 402 1778 402
Size: 1376 3957
AatII G_ACGT'C
Cuts at: 1742 1742
Size: 5333
AccI GT'mk_AC
Cuts at: 2371 2371
Size: 5333
Acc65I G'GTAC_C
Cuts at: 1264 1934 2349 1264
Size: 670 415 4248
Fragments arranged by size:
4248 670 415
AclI AA'CG_TT
Cuts at: 5173 5173
Size: 5333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3158 3590 4337 4605 3158
Size: 432 747 268 3886
Fragments arranged by size:
3886 747 432 268
AfeI AGC'GCT
Cuts at: 2254 2254
Size: 5333
AflIII A'CryG_T
Cuts at: 1886 2685 3789 4766 1886
Size: 799 1104 977 2453
Fragments arranged by size:
2453 1104 977 799
AgeI A'CCGG_T
Cuts at: 3721 3721
Size: 5333
AleI CACnn'nnGTG
Cuts at: 1907 2203 1907
Size: 296 5037
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1394 1786 2221 2238 1394
Size: 392 435 17 4489
Fragments arranged by size:
4489 435 392 17
AlwNI CAG_nnn'CTG
Cuts at: 417 879 2241 2638 4205 417
Size: 462 1362 397 1567 1545
Fragments arranged by size:
1567 1545 1362 462 397
ApaI G_GGCC'C
Cuts at: 211 460 536 1561 211
Size: 249 76 1025 3983
Fragments arranged by size:
3983 1025 249 76
ApaLI G'TGCA_C
Cuts at: 525 4103 525
Size: 3578 1755
ApoI r'AATT_y
Cuts at: 2337 4686 4942 2337
Size: 2349 256 2728
Fragments arranged by size:
2728 2349 256
AscI GG'CGCG_CC
Cuts at: 707 707
Size: 5333
AseI AT'TA_AT
Cuts at: 20 20
Size: 5333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
AvaI C'yCGr_G
Cuts at: 459 596 1216 1495 1641 2353 459
Size: 137 620 279 146 712 3439
Fragments arranged by size:
3439 712 620 279 146 137
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1256 1289 1256
Size: 33 5300
BamHI G'GATC_C
Cuts at: 1138 2358 1138
Size: 1220 4113
BanI G'GyrC_C
Cuts at: 1264 1808 1814 1934 2038 2349 2763 3011 3046
Size: 544 6 120 104 311 414 248 35
Cuts at: 3046 1264
Size: 3551
Fragments arranged by size:
3551 544 414 311 248 120 104 35
6
BanII G_rGCy'C
Cuts at: 211 392 460 536 666 1348 1561 2116 2315
Size: 181 68 76 130 682 213 555 199
Cuts at: 2315 2347 3377 211
Size: 32 1030 2167
Fragments arranged by size:
2167 1030 682 555 213 199 181 130
76 68 32
BbeI G_GCGC'C
Cuts at: 2767 3015 2767
Size: 248 5085
BbsI GAAGACnn'nnnn_
Cuts at: 1841 2090 1841
Size: 249 5084
BbvCI CC'TCA_GC
Cuts at: 493 1598 1825 493
Size: 1105 227 4001
Fragments arranged by size:
4001 1105 227
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1197 1231 90
Size: 34 1073 34 4192
Fragments arranged by size:
4192 1073 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3231 3992 5333 3231
Size: 761 1341 3231
Fragments arranged by size:
3231 1341 761
BclI T'GATC_A
Cuts at: 2853 2853
Size: 5333
BglI GCCn_nnn'nGGC
Cuts at: 317 474 685 2074 317
Size: 157 211 1389 3576
Fragments arranged by size:
3576 1389 211 157
BglII A'GATC_T
Cuts at: 2848 4885 2848
Size: 2037 3296
BlpI GC'TnA_GC
Cuts at: 541 2445 541
Size: 1904 3429
Bme1580I G_kGCm'C
Cuts at: 211 460 529 536 1561 1819 1914 2041 2958
Size: 249 69 7 1025 258 95 127 917
Cuts at: 2958 3051 4107 211
Size: 93 1056 1437
Fragments arranged by size:
1437 1056 1025 917 258 249 127 95
93 69 7
BmgBI CAC'GTC
Cuts at: 1889 1889
Size: 5333
BmrI ACTGGGnnnn_n'
Cuts at: 2545 2626 2961 5105 2545
Size: 81 335 2144 2773
Fragments arranged by size:
2773 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3700 3700
Size: 5333
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 421 716 859 1419 2127 2299 325
Size: 96 295 143 560 708 172 3359
Fragments arranged by size:
3359 708 560 295 172 143 96
Bpu10I CC'TnA_GC
Cuts at: 376 493 1099 1598 1825 376
Size: 117 606 499 227 3884
Fragments arranged by size:
3884 606 499 227 117
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 996 1528 2103 2509 3388 3880 4178 996
Size: 532 575 406 879 492 298 2151
Fragments arranged by size:
2151 879 575 532 492 406 298
BsaI GGTCTCn'nnnn_
Cuts at: 776 1304 1870 5037 776
Size: 528 566 3167 1072
Fragments arranged by size:
3167 1072 566 528
BsaAI yAC'GTr
Cuts at: 2686 2686
Size: 5333
BsaBI GATnn'nnATC
Cuts at: 6 1754 2847 4884 6
Size: 1748 1093 2037 455
Fragments arranged by size:
2037 1748 1093 455
BsaHI Gr'CG_yC
Cuts at: 350 1739 2764 3012 3714 5210 350
Size: 1389 1025 248 702 1496 473
Fragments arranged by size:
1496 1389 1025 702 473 248
BsaWI w'CCGG_w
Cuts at: 2522 3043 3721 3995 4142 4553 2522
Size: 521 678 274 147 411 3302
Fragments arranged by size:
3302 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1417 1447 1417
Size: 30 5303
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 619 1063 1496 1708 1711 1729 2124 619
Size: 444 433 212 3 18 395 3828
Fragments arranged by size:
3828 444 433 395 212 18 3
BseYI C'CCAG_C
Cuts at: 151 155 452 651 897 1175 1279 1472 1961
Size: 4 297 199 246 278 104 193 489
Cuts at: 1961 2140 2241 2307 2758 3516 4093 4589 151
Size: 179 101 66 451 758 577 496 895
Fragments arranged by size:
895 758 577 496 489 451 297 278
246 199 193 179 104 101 66 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 627 463
Size: 164 5169
BsiEI CG_ry'CG
Cuts at: 104 2875 2921 4129 104
Size: 2771 46 1208 1308
Fragments arranged by size:
2771 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 382 392 529 2347 3125 3315 4107 382
Size: 10 137 1818 778 190 792 1608
Fragments arranged by size:
1818 1608 792 778 190 137 10
Bsp1286I G_dGCh'C
Cuts at: 211 382 392 460 529 536 666 1348 1561
Size: 171 10 68 69 7 130 682 213
Cuts at: 1561 1819 1914 2041 2116 2315 2347 2958 3051
Size: 258 95 127 75 199 32 611 93
Cuts at: 3051 3125 3315 3377 4107 211
Size: 74 190 62 730 1437
Fragments arranged by size:
1437 730 682 611 258 213 199 190
171 130 127 95 93 75 74 69
68 62 32 10 7
BspEI T'CCGG_A
Cuts at: 4553 4553
Size: 5333
BspHI T'CATG_A
Cuts at: 4509 4509
Size: 5333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 371 402 794 878 1411 1778 2383 2597 2899
Size: 31 392 84 533 367 605 214 302
Cuts at: 2899 3280 371
Size: 381 2424
Fragments arranged by size:
2424 605 533 392 381 367 302 214
84 31
BsrBI CCG'CTC
Cuts at: 50 186 514 1247 3625 3679 5163 5328 50
Size: 136 328 733 2378 54 1484 165 55
Fragments arranged by size:
2378 1484 733 328 165 136 55 54
BsrDI GCAATG_nn'
Cuts at: 3245 3245
Size: 5333
BsrFI r'CCGG_y
Cuts at: 468 1811 3331 3512 3721 468
Size: 1343 1520 181 209 2080
Fragments arranged by size:
2080 1520 1343 209 181
BssHII G'CGCG_C
Cuts at: 369 707 1124 369
Size: 338 417 4578
Fragments arranged by size:
4578 417 338
BssSI C'ACGA_G
Cuts at: 1572 3604 3962 1572
Size: 2032 358 2943
Fragments arranged by size:
2943 2032 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2710 2710
Size: 5333
BstBI TT'CG_AA
Cuts at: 3694 3694
Size: 5333
BstXI CCAn_nnnn'nTGG
Cuts at: 1539 2202 1539
Size: 663 4670
BstYI r'GATC_y
Cuts at: 1 735 1138 1940 2358 2825 2848 3183 3429
Size: 734 403 802 418 467 23 335 246
Cuts at: 3429 4441 4527 4539 4885 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 802 734 467 449 418 403 346
335 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 811 811
Size: 5333
BtgI C'CryG_G
Cuts at: 266 1880 2000 2092 2201 266
Size: 1614 120 92 109 3398
Fragments arranged by size:
3398 1614 120 109 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 423 660 1391 3283 3464 3627 423
Size: 237 731 1892 181 163 2129
Fragments arranged by size:
2129 1892 731 237 181 163
BtsI GCAGTG_nn'
Cuts at: 228 334 2576 228
Size: 106 2242 2985
Fragments arranged by size:
2985 2242 106
ClaI AT'CG_AT
Cuts at: 1021 1021
Size: 5333
DraI TTT'AAA
Cuts at: 2332 2332
Size: 5333
DraIII CAC_nnn'GTG
Cuts at: 1121 2271 1121
Size: 1150 4183
DrdI GACnn_nn'nnGTC
Cuts at: 3039 3897 3039
Size: 858 4475
EaeI y'GGCC_r
Cuts at: 393 509 1366 1541 1605 2066 2918 3092 3483
Size: 116 857 175 64 461 852 174 391
Cuts at: 3483 3510 4855 393
Size: 27 1345 871
Fragments arranged by size:
1345 871 857 852 461 391 175 174
116 64 27
EagI C'GGCC_G
Cuts at: 2918 2918
Size: 5333
EarI CTCTTCn'nnn_
Cuts at: 193 3356 3566 193
Size: 3163 210 1960
Fragments arranged by size:
3163 1960 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3541 3851 3997 5143 3541
Size: 310 146 1146 3731
Fragments arranged by size:
3731 1146 310 146
EcoICRI GAG'CTC
Cuts at: 390 2345 390
Size: 1955 3378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 421 716 859 1419 2127 2299 3158 3590
Size: 96 295 143 560 708 172 859 432
Cuts at: 3590 4337 4605 325
Size: 747 268 1053
Fragments arranged by size:
1053 859 747 708 560 432 295 268
172 143 96
EcoO109I rG'GnC_Cy
Cuts at: 180 207 208 456 951 1014 1332 1469 1558
Size: 27 1 248 495 63 318 137 89
Cuts at: 1558 2472 180
Size: 914 3041
Fragments arranged by size:
3041 914 495 318 248 137 89 63
27 1
EcoRI G'AATT_C
Cuts at: 2337 2337
Size: 5333
EcoRV GAT'ATC
Cuts at: 1518 1518
Size: 5333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2160 3352 2160
Size: 1192 4141
FspI TGC'GCA
Cuts at: 340 3114 340
Size: 2774 2559
HaeII r_GCGC'y
Cuts at: 312 565 589 1897 2256 2767 3015 4037 5016
Size: 253 24 1308 359 511 248 1022 979
Cuts at: 5016 312
Size: 629
Fragments arranged by size:
1308 1022 979 629 511 359 253 248
24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 3175 3205 3207 3237 3175
Size: 30 2 30 5271
Fragments arranged by size:
5271 30 30 2
HincII GTy'rAC
Cuts at: 2372 2372
Size: 5333
KasI G'GCGC_C
Cuts at: 2763 3011 2763
Size: 248 5085
KpnI G_GTAC'C
Cuts at: 1268 1938 2353 1268
Size: 670 415 4248
Fragments arranged by size:
4248 670 415
MluI A'CGCG_T
Cuts at: 4766 4766
Size: 5333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1223 4004 4188 1223
Size: 2781 184 2368
Fragments arranged by size:
2781 2368 184
MscI TGG'CCA
Cuts at: 1543 3094 1543
Size: 1551 3782
MslI CAynn'nnrTG
Cuts at: 1376 1907 2203 3322 3449 1376
Size: 531 296 1119 127 3260
Fragments arranged by size:
3260 1119 531 296 127
MspA1I CmG'CkG
Cuts at: 126 155 268 408 507 579 743 879 1132
Size: 29 113 140 99 72 164 136 253
Cuts at: 1132 1145 1179 1525 1829 2180 2307 2444 2758
Size: 13 34 346 304 351 127 137 314
Cuts at: 2758 3118 4131 4376 126
Size: 360 1013 245 1083
Fragments arranged by size:
1083 1013 360 351 346 314 304 253
245 164 140 137 136 127 113 99
72 34 29 13
NaeI GCC'GGC
Cuts at: 470 3514 470
Size: 3044 2289
NarI GG'CG_CC
Cuts at: 2764 3012 2764
Size: 248 5085
NgoMIV G'CCGG_C
Cuts at: 468 3512 468
Size: 3044 2289
NspI r_CATG'y
Cuts at: 1492 2219 2386 2886 3793 1492
Size: 727 167 500 907 3032
Fragments arranged by size:
3032 907 727 500 167
PasI CC'CwG_GG
Cuts at: 212 666 1448 212
Size: 454 782 4097
Fragments arranged by size:
4097 782 454
PciI A'CATG_T
Cuts at: 3789 3789
Size: 5333
PflMI CCAn_nnn'nTGG
Cuts at: 1980 2241 1980
Size: 261 5072
PfoI T'CCnGG_A
Cuts at: 731 731
Size: 5333
PmeI GTTT'AAAC
Cuts at: 2332 2332
Size: 5333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 673 705 4472 4504 673
Size: 32 3767 32 1502
Fragments arranged by size:
3767 1502 32 32
PpuMI rG'GwC_Cy
Cuts at: 180 951 1014 1469 180
Size: 771 63 455 4044
Fragments arranged by size:
4044 771 455 63
PspOMI G'GGCC_C
Cuts at: 207 456 532 1557 207
Size: 249 76 1025 3983
Fragments arranged by size:
3983 1025 249 76
PstI C_TGCA'G
Cuts at: 413 593 875 1775 1796 1865 2227 2252 2380
Size: 180 282 900 21 69 362 25 128
Cuts at: 2380 3065 413
Size: 685 2681
Fragments arranged by size:
2681 900 685 362 282 180 128 69
25 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5333
PvuII CAG'CTG
Cuts at: 155 408 507 879 1132 1179 1525 2758 3118
Size: 253 99 372 253 47 346 1233 360
Cuts at: 3118 155
Size: 2370
Fragments arranged by size:
2370 1233 372 360 346 253 253 99
47
RsrII CG'GwC_CG
Cuts at: 3528 3528
Size: 5333
SacI G_AGCT'C
Cuts at: 392 2347 392
Size: 1955 3378
SacII CC_GC'GG
Cuts at: 269 269
Size: 5333
SalI G'TCGA_C
Cuts at: 2370 2370
Size: 5333
SapI GCTCTTCn'nnn_
Cuts at: 193 3356 3566 193
Size: 3163 210 1960
Fragments arranged by size:
3163 1960 210
SbfI CC_TGCA'GG
Cuts at: 2380 2380
Size: 5333
ScaI AGT'ACT
Cuts at: 4850 4850
Size: 5333
SexAI A'CCwGG_T
Cuts at: 2267 2267
Size: 5333
SfcI C'TryA_G
Cuts at: 33 409 589 871 946 1415 1686 1771 1792
Size: 376 180 282 75 469 271 85 21
Cuts at: 1792 1861 2223 2248 2376 3061 4054 4245 33
Size: 69 362 25 128 685 993 191 1121
Fragments arranged by size:
1121 993 685 469 376 362 282 271
191 180 128 85 75 69 25 21
SfoI GGC'GCC
Cuts at: 2765 3013 2765
Size: 248 5085
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
SmaI CCC'GGG
Cuts at: 461 598 2355 461
Size: 137 1757 3439
Fragments arranged by size:
3439 1757 137
SmlI C'TyrA_G
Cuts at: 975 1507 2082 2488 3403 3895 4157 975
Size: 532 575 406 915 492 262 2151
Fragments arranged by size:
2151 915 575 532 492 406 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5333
SphI G_CATG'C
Cuts at: 2386 2886 2386
Size: 500 4833
StyI C'CwwG_G
Cuts at: 991 1328 2467 2821 991
Size: 337 1139 354 3503
Fragments arranged by size:
3503 1139 354 337
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1244 2916 3049 3508 3717 1244
Size: 1672 133 459 209 2860
Fragments arranged by size:
2860 1672 459 209 133
TatI w'GTAC_w
Cuts at: 4848 4848
Size: 5333
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1417 1449 4850 4882 1417
Size: 32 3401 32 1868
Fragments arranged by size:
3401 1868 32 32
Tth111I GACn'n_nGTC
Cuts at: 3130 3130
Size: 5333
XbaI T'CTAG_A
Cuts at: 2364 2364
Size: 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1551 2828 1551
Size: 1277 4056
XmaI C'CCGG_G
Cuts at: 459 596 2353 459
Size: 137 1757 3439
Fragments arranged by size:
3439 1757 137
ZraI GAC'GTC
Cuts at: 1740 1740
Size: 5333
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AloI AvrII BfrBI BmtI BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I CspCI EcoNI FseI FspAI
HindIII HpaI MfeI NcoI NdeI NheI NotI NruI
NsiI PacI PmlI PshAI PsiI PspXI PsrI SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI