Restriction map for
pF1KB5748
(Circular) (Six-base) MAPSORT of: pF1KB5748.seq Check: 5779 from: 1 to: 5351
pF1KB5748 5351 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1006 1006
Size: 5351
AccI GT'mk_AC
Cuts at: 455 461 2389 455
Size: 6 1928 3417
Fragments arranged by size:
3417 1928 6
Acc65I G'GTAC_C
Cuts at: 917 1880 2367 917
Size: 963 487 3901
Fragments arranged by size:
3901 963 487
AclI AA'CG_TT
Cuts at: 5191 5191
Size: 5351
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1125 1235 1343 2053 3176 3608 4355 4623 1125
Size: 110 108 710 1123 432 747 268 1853
Fragments arranged by size:
1853 1123 747 710 432 268 110 108
AflIII A'CryG_T
Cuts at: 2703 3807 4784 2703
Size: 1104 977 3270
Fragments arranged by size:
3270 1104 977
AgeI A'CCGG_T
Cuts at: 3739 3739
Size: 5351
AhdI GACnn_n'nnGTC
Cuts at: 603 603
Size: 5351
AleI CACnn'nnGTG
Cuts at: 1705 1705
Size: 5351
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1939 1971 1939
Size: 32 5319
AlwNI CAG_nnn'CTG
Cuts at: 1975 2656 4223 1975
Size: 681 1567 3103
Fragments arranged by size:
3103 1567 681
ApaLI G'TGCA_C
Cuts at: 340 619 4121 340
Size: 279 3502 1570
Fragments arranged by size:
3502 1570 279
ApoI r'AATT_y
Cuts at: 1543 2355 4704 4960 1543
Size: 812 2349 256 1934
Fragments arranged by size:
2349 1934 812 256
AseI AT'TA_AT
Cuts at: 20 1223 20
Size: 1203 4148
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
AvaI C'yCGr_G
Cuts at: 2168 2371 2168
Size: 203 5148
BamHI G'GATC_C
Cuts at: 2376 2376
Size: 5351
BanI G'GyrC_C
Cuts at: 917 1880 2367 2781 3029 3064 917
Size: 963 487 414 248 35 3204
Fragments arranged by size:
3204 963 487 414 248 35
BanII G_rGCy'C
Cuts at: 529 2365 3395 529
Size: 1836 1030 2485
Fragments arranged by size:
2485 1836 1030
BbeI G_GCGC'C
Cuts at: 2785 3033 2785
Size: 248 5103
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 2198 2232 90
Size: 34 2074 34 3209
Fragments arranged by size:
3209 2074 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 576 1921 3249 4010 5351 576
Size: 1345 1328 761 1341 576
Fragments arranged by size:
1345 1341 1328 761 576
BclI T'GATC_A
Cuts at: 2871 2871
Size: 5351
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5351
BglII A'GATC_T
Cuts at: 2866 4903 2866
Size: 2037 3314
BlpI GC'TnA_GC
Cuts at: 1186 1792 2463 1186
Size: 606 671 4074
Fragments arranged by size:
4074 671 606
Bme1580I G_kGCm'C
Cuts at: 344 623 1641 2976 3069 4125 344
Size: 279 1018 1335 93 1056 1570
Fragments arranged by size:
1570 1335 1056 1018 279 93
BmgBI CAC'GTC
Cuts at: 726 963 726
Size: 237 5114
BmrI ACTGGGnnnn_n'
Cuts at: 248 1623 2563 2644 2979 5123 248
Size: 1375 940 81 335 2144 476
Fragments arranged by size:
2144 1375 940 476 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 5351
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 513 1171 1475 360
Size: 153 658 304 4236
Fragments arranged by size:
4236 658 304 153
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2527 3406 3898 4196 2527
Size: 879 492 298 3682
Fragments arranged by size:
3682 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 590 1584 2310 5055 590
Size: 994 726 2745 886
Fragments arranged by size:
2745 994 886 726
BsaAI yAC'GTr
Cuts at: 459 1426 2704 459
Size: 967 1278 3106
Fragments arranged by size:
3106 1278 967
BsaBI GATnn'nnATC
Cuts at: 6 1932 2865 4902 6
Size: 1926 933 2037 455
Fragments arranged by size:
2037 1926 933 455
BsaHI Gr'CG_yC
Cuts at: 1784 2782 3030 3732 5228 1784
Size: 998 248 702 1496 1907
Fragments arranged by size:
1907 1496 998 702 248
BsaWI w'CCGG_w
Cuts at: 258 2540 3061 3739 4013 4160 4571 258
Size: 2282 521 678 274 147 411 1038
Fragments arranged by size:
2282 1038 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 838 861 868 891 1939 1969 1993 2023 838
Size: 23 7 23 1048 30 24 30 4166
Fragments arranged by size:
4166 1048 30 30 24 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 216 869 979 216
Size: 653 110 4588
Fragments arranged by size:
4588 653 110
BseYI C'CCAG_C
Cuts at: 550 1589 2204 2776 3534 4111 4607 550
Size: 1039 615 572 758 577 496 1294
Fragments arranged by size:
1294 1039 758 615 577 572 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 661 1075 661
Size: 414 4937
BsiEI CG_ry'CG
Cuts at: 104 2893 2939 4147 104
Size: 2789 46 1208 1308
Fragments arranged by size:
2789 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 344 623 989 1793 2365 3143 3333 4125 344
Size: 279 366 804 572 778 190 792 1570
Fragments arranged by size:
1570 804 792 778 572 366 279 190
BsmI GAATG_Cn'
Cuts at: 954 954
Size: 5351
Bsp1286I G_dGCh'C
Cuts at: 344 529 623 989 1641 1793 2365 2976 3069
Size: 185 94 366 652 152 572 611 93
Cuts at: 3069 3143 3333 3395 4125 344
Size: 74 190 62 730 1570
Fragments arranged by size:
1570 730 652 611 572 366 190 185
152 94 93 74 62
BspEI T'CCGG_A
Cuts at: 258 4571 258
Size: 4313 1038
BspHI T'CATG_A
Cuts at: 2075 4527 2075
Size: 2452 2899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1006 2027 2401 2615 2917 3298 1006
Size: 1021 374 214 302 381 3059
Fragments arranged by size:
3059 1021 381 374 302 214
BsrBI CCG'CTC
Cuts at: 50 1920 3643 3697 5181 5346 50
Size: 1870 1723 54 1484 165 55
Fragments arranged by size:
1870 1723 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1197 3263 1197
Size: 2066 3285
BsrFI r'CCGG_y
Cuts at: 3349 3530 3739 3349
Size: 181 209 4961
Fragments arranged by size:
4961 209 181
BssHII G'CGCG_C
Cuts at: 1888 1888
Size: 5351
BssSI C'ACGA_G
Cuts at: 1118 1609 3622 3980 1118
Size: 491 2013 358 2489
Fragments arranged by size:
2489 2013 491 358
BstAPI GCAn_nnn'nTGC
Cuts at: 398 2728 398
Size: 2330 3021
BstBI TT'CG_AA
Cuts at: 3712 3712
Size: 5351
BstEII G'GTnAC_C
Cuts at: 1566 1566
Size: 5351
BstYI r'GATC_y
Cuts at: 1 631 2376 2843 2866 3201 3447 4459 4545
Size: 630 1745 467 23 335 246 1012 86
Cuts at: 4545 4557 4903 1
Size: 12 346 449
Fragments arranged by size:
1745 1012 630 467 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 462 462
Size: 5351
BtgI C'CryG_G
Cuts at: 1958 1958
Size: 5351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 763 1265 3301 3482 3645 763
Size: 502 2036 181 163 2469
Fragments arranged by size:
2469 2036 502 181 163
BtsI GCAGTG_nn'
Cuts at: 1547 1700 2594 1547
Size: 153 894 4304
Fragments arranged by size:
4304 894 153
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1321 1356 1811 1846 1321
Size: 35 455 35 4826
Fragments arranged by size:
4826 455 35 35
DraI TTT'AAA
Cuts at: 1529 2295 2350 1529
Size: 766 55 4530
Fragments arranged by size:
4530 766 55
DraIII CAC_nnn'GTG
Cuts at: 1850 1850
Size: 5351
DrdI GACnn_nn'nnGTC
Cuts at: 3057 3915 3057
Size: 858 4493
EaeI y'GGCC_r
Cuts at: 515 741 755 2054 2936 3110 3501 3528 4873
Size: 226 14 1299 882 174 391 27 1345
Cuts at: 4873 515
Size: 993
Fragments arranged by size:
1345 1299 993 882 391 226 174 27
14
EagI C'GGCC_G
Cuts at: 2936 2936
Size: 5351
EarI CTCTTCn'nnn_
Cuts at: 907 3374 3584 907
Size: 2467 210 2674
Fragments arranged by size:
2674 2467 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3559 3869 4015 5161 3559
Size: 310 146 1146 3749
Fragments arranged by size:
3749 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2363 2363
Size: 5351
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 360 513 1125 1171 1235 1343 1475 2053 3176
Size: 153 612 46 64 108 132 578 1123
Cuts at: 3176 3608 4355 4623 360
Size: 432 747 268 1088
Fragments arranged by size:
1123 1088 747 612 578 432 268 153
132 108 64 46
EcoNI CCTnn'n_nnAGG
Cuts at: 1801 1801
Size: 5351
EcoO109I rG'GnC_Cy
Cuts at: 1136 2490 1136
Size: 1354 3997
EcoRI G'AATT_C
Cuts at: 2355 2355
Size: 5351
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3370 3370
Size: 5351
FspI TGC'GCA
Cuts at: 3132 3132
Size: 5351
HaeII r_GCGC'y
Cuts at: 2785 3033 4055 5034 2785
Size: 248 1022 979 3102
Fragments arranged by size:
3102 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 358 390 589 621 2250 2282 2290 2322 3193
Size: 32 199 32 1629 32 8 32 871
Cuts at: 3193 3223 3225 3255 358
Size: 30 2 30 2454
Fragments arranged by size:
2454 1629 871 199 32 32 32 32
30 30 8 2
HincII GTy'rAC
Cuts at: 1967 2390 1967
Size: 423 4928
HindIII A'AGCT_T
Cuts at: 1603 1603
Size: 5351
KasI G'GCGC_C
Cuts at: 2781 3029 2781
Size: 248 5103
KpnI G_GTAC'C
Cuts at: 921 1884 2371 921
Size: 963 487 3901
Fragments arranged by size:
3901 963 487
MfeI C'AATT_G
Cuts at: 316 2050 2058 316
Size: 1734 8 3609
Fragments arranged by size:
3609 1734 8
MluI A'CGCG_T
Cuts at: 4784 4784
Size: 5351
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 810 4022 4206 810
Size: 3212 184 1955
Fragments arranged by size:
3212 1955 184
MscI TGG'CCA
Cuts at: 757 2056 3112 757
Size: 1299 1056 2996
Fragments arranged by size:
2996 1299 1056
MslI CAynn'nnrTG
Cuts at: 1705 1938 2074 3340 3467 1705
Size: 233 136 1266 127 3589
Fragments arranged by size:
3589 1266 233 136 127
MspA1I CmG'CkG
Cuts at: 145 230 1691 2237 2256 2462 2776 3136 4149
Size: 85 1461 546 19 206 314 360 1013
Cuts at: 4149 4394 145
Size: 245 1102
Fragments arranged by size:
1461 1102 1013 546 360 314 245 206
85 19
NaeI GCC'GGC
Cuts at: 3532 3532
Size: 5351
NarI GG'CG_CC
Cuts at: 2782 3030 2782
Size: 248 5103
NcoI C'CATG_G
Cuts at: 1958 1958
Size: 5351
NgoMIV G'CCGG_C
Cuts at: 3530 3530
Size: 5351
NspI r_CATG'y
Cuts at: 897 2253 2404 2904 3811 897
Size: 1356 151 500 907 2437
Fragments arranged by size:
2437 1356 907 500 151
PciI A'CATG_T
Cuts at: 3807 3807
Size: 5351
PfoI T'CCnGG_A
Cuts at: 281 1148 1452 281
Size: 867 304 4180
Fragments arranged by size:
4180 867 304
PmeI GTTT'AAAC
Cuts at: 2350 2350
Size: 5351
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1939 1971 4490 4522 1939
Size: 32 2519 32 2768
Fragments arranged by size:
2768 2519 32 32
PpuMI rG'GwC_Cy
Cuts at: 1136 1136
Size: 5351
PstI C_TGCA'G
Cuts at: 1554 1620 2398 3083 1554
Size: 66 778 685 3822
Fragments arranged by size:
3822 778 685 66
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5351
PvuII CAG'CTG
Cuts at: 145 2237 2776 3136 145
Size: 2092 539 360 2360
Fragments arranged by size:
2360 2092 539 360
RsrII CG'GwC_CG
Cuts at: 3546 3546
Size: 5351
SacI G_AGCT'C
Cuts at: 2365 2365
Size: 5351
SalI G'TCGA_C
Cuts at: 2388 2388
Size: 5351
SapI GCTCTTCn'nnn_
Cuts at: 3374 3584 3374
Size: 210 5141
SbfI CC_TGCA'GG
Cuts at: 2398 2398
Size: 5351
ScaI AGT'ACT
Cuts at: 774 1633 4868 774
Size: 859 3235 1257
Fragments arranged by size:
3235 1257 859
SexAI A'CCwGG_T
Cuts at: 2300 2300
Size: 5351
SfcI C'TryA_G
Cuts at: 33 1550 1616 2394 3079 4072 4263 33
Size: 1517 66 778 685 993 191 1121
Fragments arranged by size:
1517 1121 993 778 685 191 66
SfoI GGC'GCC
Cuts at: 2783 3031 2783
Size: 248 5103
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5351
SmaI CCC'GGG
Cuts at: 2373 2373
Size: 5351
SmlI C'TyrA_G
Cuts at: 2506 3421 3913 4175 2506
Size: 915 492 262 3682
Fragments arranged by size:
3682 915 492 262
SnaBI TAC'GTA
Cuts at: 459 459
Size: 5351
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5351
SphI G_CATG'C
Cuts at: 2404 2904 2404
Size: 500 4851
SspI AAT'ATT
Cuts at: 1409 1993 1409
Size: 584 4767
StyI C'CwwG_G
Cuts at: 307 1958 2485 2839 307
Size: 1651 527 354 2819
Fragments arranged by size:
2819 1651 527 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2934 3067 3526 3735 2934
Size: 133 459 209 4550
Fragments arranged by size:
4550 459 209 133
TatI w'GTAC_w
Cuts at: 772 890 1631 4866 772
Size: 118 741 3235 1257
Fragments arranged by size:
3235 1257 741 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 861 893 4868 4900 861
Size: 32 3975 32 1312
Fragments arranged by size:
3975 1312 32 32
Tth111I GACn'n_nGTC
Cuts at: 2284 2302 3148 2284
Size: 18 846 4487
Fragments arranged by size:
4487 846 18
XbaI T'CTAG_A
Cuts at: 2382 2382
Size: 5351
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1107 2846 1107
Size: 1739 3612
XmaI C'CCGG_G
Cuts at: 2371 2371
Size: 5351
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmtI Bpu10I BsiWI BsmBI BsrGI
BstXI Bsu36I ClaI EcoRV FseI FspAI HpaI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SrfI StuI SwaI XhoI XmnI ZraI