Restriction map for
pF1KB5751
(Circular) (Six-base) MAPSORT of: pF1KB5751.seq Check: 5101 from: 1 to: 5537
pF1KB5751 5537 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 538 1341 2016 538
Size: 803 675 4059
Fragments arranged by size:
4059 803 675
AccI GT'mk_AC
Cuts at: 2195 2575 2195
Size: 380 5157
Acc65I G'GTAC_C
Cuts at: 211 1211 1331 2553 211
Size: 1000 120 1222 3195
Fragments arranged by size:
3195 1222 1000 120
AclI AA'CG_TT
Cuts at: 5377 5377
Size: 5537
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 3362 3794 4541 4809 207
Size: 3155 432 747 268 935
Fragments arranged by size:
3155 935 747 432 268
AflIII A'CryG_T
Cuts at: 1160 2889 3993 4970 1160
Size: 1729 1104 977 1727
Fragments arranged by size:
1729 1727 1104 977
AgeI A'CCGG_T
Cuts at: 3925 3925
Size: 5537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2048 2531 2048
Size: 483 5054
AlwNI CAG_nnn'CTG
Cuts at: 1341 1857 1930 1947 1974 2131 2340 2842 4409
Size: 516 73 17 27 157 209 502 1567
Cuts at: 4409 1341
Size: 2469
Fragments arranged by size:
2469 1567 516 502 209 157 73 27
17
ApaI G_GGCC'C
Cuts at: 2151 2151
Size: 5537
ApaLI G'TGCA_C
Cuts at: 1054 4307 1054
Size: 3253 2284
ApoI r'AATT_y
Cuts at: 2541 4890 5146 2541
Size: 2349 256 2932
Fragments arranged by size:
2932 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5537
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
AvaI C'yCGr_G
Cuts at: 652 757 1145 2249 2557 652
Size: 105 388 1104 308 3632
Fragments arranged by size:
3632 1104 388 308 105
BamHI G'GATC_C
Cuts at: 2562 2562
Size: 5537
BanI G'GyrC_C
Cuts at: 211 320 429 550 771 1211 1331 2553 2967
Size: 109 109 121 221 440 120 1222 414
Cuts at: 2967 3215 3250 211
Size: 248 35 2498
Fragments arranged by size:
2498 1222 440 414 248 221 121 120
109 109 35
BanII G_rGCy'C
Cuts at: 165 651 832 1708 2151 2551 3581 165
Size: 486 181 876 443 400 1030 2121
Fragments arranged by size:
2121 1030 876 486 443 400 181
BbeI G_GCGC'C
Cuts at: 2971 3219 2971
Size: 248 5289
BbsI GAAGACnn'nnnn_
Cuts at: 2062 2062
Size: 5537
BbvCI CC'TCA_GC
Cuts at: 2395 2395
Size: 5537
BciVI GTATCCnnnnn_n'
Cuts at: 3435 4196 5537 3435
Size: 761 1341 3435
Fragments arranged by size:
3435 1341 761
BclI T'GATC_A
Cuts at: 1283 3057 1283
Size: 1774 3763
BglI GCCn_nnn'nGGC
Cuts at: 1393 1393
Size: 5537
BglII A'GATC_T
Cuts at: 3052 5089 3052
Size: 2037 3500
BlpI GC'TnA_GC
Cuts at: 113 945 2649 113
Size: 832 1704 3001
Fragments arranged by size:
3001 1704 832
Bme1580I G_kGCm'C
Cuts at: 434 555 776 1058 2151 2304 3162 3255 4311
Size: 121 221 282 1093 153 858 93 1056
Cuts at: 4311 434
Size: 1660
Fragments arranged by size:
1660 1093 1056 858 282 221 153 121
93
BmgBI CAC'GTC
Cuts at: 1163 1163
Size: 5537
BmrI ACTGGGnnnn_n'
Cuts at: 393 604 693 1751 2505 2749 2830 3165 5309
Size: 211 89 1058 754 244 81 335 2144
Cuts at: 5309 393
Size: 621
Fragments arranged by size:
2144 1058 754 621 335 244 211 89
81
BmtI G_CTAG'C
Cuts at: 296 296
Size: 5537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 361 654 1196 2379 3904 361
Size: 293 542 1183 1525 1994
Fragments arranged by size:
1994 1525 1183 542 293
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 2510 1030
Size: 1480 4057
Bpu10I CC'TnA_GC
Cuts at: 472 2354 2395 472
Size: 1882 41 3614
Fragments arranged by size:
3614 1882 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2713 3592 4084 4382 2713
Size: 879 492 298 3868
Fragments arranged by size:
3868 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5241 5241
Size: 5537
BsaAI yAC'GTr
Cuts at: 1053 2890 1053
Size: 1837 3700
BsaBI GATnn'nnATC
Cuts at: 6 3051 5088 6
Size: 3045 2037 455
Fragments arranged by size:
3045 2037 455
BsaHI Gr'CG_yC
Cuts at: 275 2968 3216 3918 5414 275
Size: 2693 248 702 1496 398
Fragments arranged by size:
2693 1496 702 398 248
BsaWI w'CCGG_w
Cuts at: 395 2261 2726 3247 3925 4199 4346 4757 395
Size: 1866 465 521 678 274 147 411 1175
Fragments arranged by size:
1866 1175 678 521 465 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1093 1123 1093
Size: 30 5507
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 867 1450 1698 867
Size: 583 248 4706
Fragments arranged by size:
4706 583 248
BseYI C'CCAG_C
Cuts at: 183 354 479 495 2203 2387 2962 3720 4297
Size: 171 125 16 1708 184 575 758 577
Cuts at: 4297 4793 183
Size: 496 927
Fragments arranged by size:
1708 927 758 577 575 496 184 171
125 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 664 1022 1369 2002 2527 664
Size: 358 347 633 525 3674
Fragments arranged by size:
3674 633 525 358 347
BsiEI CG_ry'CG
Cuts at: 104 261 3079 3125 4333 104
Size: 157 2818 46 1208 1308
Fragments arranged by size:
2818 1308 1208 157 46
BsiHKAI G_wGCw'C
Cuts at: 491 1058 1924 2009 2551 3329 3519 4311 491
Size: 567 866 85 542 778 190 792 1717
Fragments arranged by size:
1717 866 792 778 567 542 190 85
BsmBI CGTCTCn'nnnn_
Cuts at: 829 1096 829
Size: 267 5270
Bsp1286I G_dGCh'C
Cuts at: 165 434 491 555 651 776 832 1058 1708
Size: 269 57 64 96 125 56 226 650
Cuts at: 1708 1924 2009 2151 2304 2551 3162 3255 3329
Size: 216 85 142 153 247 611 93 74
Cuts at: 3329 3519 3581 4311 165
Size: 190 62 730 1391
Fragments arranged by size:
1391 730 650 611 269 247 226 216
190 153 142 125 96 93 85 74
64 62 57 56
BspEI T'CCGG_A
Cuts at: 395 2261 4757 395
Size: 1866 2496 1175
Fragments arranged by size:
2496 1866 1175
BspHI T'CATG_A
Cuts at: 4713 4713
Size: 5537
BspMI ACCTGCnnnn'nnnn_
Cuts at: 538 1341 2016 2024 2587 2801 3103 3484 538
Size: 803 675 8 563 214 302 381 2591
Fragments arranged by size:
2591 803 675 563 381 302 214 8
BsrBI CCG'CTC
Cuts at: 50 310 2358 2369 3829 3883 5367 5532 50
Size: 260 2048 11 1460 54 1484 165 55
Fragments arranged by size:
2048 1484 1460 260 165 55 54 11
BsrDI GCAATG_nn'
Cuts at: 3449 3449
Size: 5537
BsrFI r'CCGG_y
Cuts at: 3535 3716 3925 3535
Size: 181 209 5147
Fragments arranged by size:
5147 209 181
BssSI C'ACGA_G
Cuts at: 3808 4166 3808
Size: 358 5179
BstAPI GCAn_nnn'nTGC
Cuts at: 2914 2914
Size: 5537
BstBI TT'CG_AA
Cuts at: 1419 3898 1419
Size: 2479 3058
BstEII G'GTnAC_C
Cuts at: 1275 1587 1275
Size: 312 5225
BstXI CCAn_nnnn'nTGG
Cuts at: 1320 1383 2304 1320
Size: 63 921 4553
Fragments arranged by size:
4553 921 63
BstYI r'GATC_y
Cuts at: 1 1028 2562 3029 3052 3387 3633 4645 4731
Size: 1027 1534 467 23 335 246 1012 86
Cuts at: 4731 4743 5089 1
Size: 12 346 449
Fragments arranged by size:
1534 1027 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 514 2174 514
Size: 1660 3877
BtgI C'CryG_G
Cuts at: 107 315 659 1043 1271 2501 107
Size: 208 344 384 228 1230 3143
Fragments arranged by size:
3143 1230 384 344 228 208
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3487 3668 3831 3487
Size: 181 163 5193
Fragments arranged by size:
5193 181 163
BtsI GCAGTG_nn'
Cuts at: 445 727 1010 1614 2042 2243 2780 445
Size: 282 283 604 428 201 537 3202
Fragments arranged by size:
3202 604 537 428 283 282 201
DraI TTT'AAA
Cuts at: 2536 2536
Size: 5537
DraIII CAC_nnn'GTG
Cuts at: 1759 1759
Size: 5537
DrdI GACnn_nn'nnGTC
Cuts at: 3243 4101 3243
Size: 858 4679
EaeI y'GGCC_r
Cuts at: 258 893 1385 1403 3122 3296 3687 3714 5059
Size: 635 492 18 1719 174 391 27 1345
Cuts at: 5059 258
Size: 736
Fragments arranged by size:
1719 1345 736 635 492 391 174 27
18
EagI C'GGCC_G
Cuts at: 258 3122 258
Size: 2864 2673
EarI CTCTTCn'nnn_
Cuts at: 1912 3560 3770 1912
Size: 1648 210 3679
Fragments arranged by size:
3679 1648 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3745 4055 4201 5347 3745
Size: 310 146 1146 3935
Fragments arranged by size:
3935 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2549 2549
Size: 5537
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 207 1030 2510 3362 3794 4541 4809 207
Size: 823 1480 852 432 747 268 935
Fragments arranged by size:
1480 935 852 823 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1096 1096
Size: 5537
EcoO109I rG'GnC_Cy
Cuts at: 227 500 510 1788 1900 2147 2676 227
Size: 273 10 1278 112 247 529 3088
Fragments arranged by size:
3088 1278 529 273 247 112 10
EcoRI G'AATT_C
Cuts at: 2541 2541
Size: 5537
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3556 3556
Size: 5537
FspI TGC'GCA
Cuts at: 3318 3318
Size: 5537
HaeII r_GCGC'y
Cuts at: 1894 2444 2971 3219 4241 5220 1894
Size: 550 527 248 1022 979 2211
Fragments arranged by size:
2211 1022 979 550 527 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 620 652 1147 1176 1179 1208 1384 1416 2243
Size: 32 495 29 3 29 176 32 827
Cuts at: 2243 2275 2429 2461 3379 3409 3411 3441 620
Size: 32 154 32 918 30 2 30 2716
Fragments arranged by size:
2716 918 827 495 176 154 32 32
32 32 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 1518 1769 2576 1518
Size: 251 807 4479
Fragments arranged by size:
4479 807 251
HindIII A'AGCT_T
Cuts at: 1422 1422
Size: 5537
KasI G'GCGC_C
Cuts at: 2967 3215 2967
Size: 248 5289
KpnI G_GTAC'C
Cuts at: 215 1215 1335 2557 215
Size: 1000 120 1222 3195
Fragments arranged by size:
3195 1222 1000 120
MluI A'CGCG_T
Cuts at: 4970 4970
Size: 5537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1140 1958 4208 4392 1140
Size: 818 2250 184 2285
Fragments arranged by size:
2285 2250 818 184
MscI TGG'CCA
Cuts at: 895 1387 3298 895
Size: 492 1911 3134
Fragments arranged by size:
3134 1911 492
MslI CAynn'nnrTG
Cuts at: 3526 3653 3526
Size: 127 5410
MspA1I CmG'CkG
Cuts at: 317 326 479 661 724 1584 1971 2128 2214
Size: 9 153 182 63 860 387 157 86
Cuts at: 2214 2648 2962 3322 4335 4580 317
Size: 434 314 360 1013 245 1274
Fragments arranged by size:
1274 1013 860 434 387 360 314 245
182 157 153 86 63 9
NaeI GCC'GGC
Cuts at: 3718 3718
Size: 5537
NarI GG'CG_CC
Cuts at: 2968 3216 2968
Size: 248 5289
NcoI C'CATG_G
Cuts at: 107 1043 1271 2501 107
Size: 936 228 1230 3143
Fragments arranged by size:
3143 1230 936 228
NgoMIV G'CCGG_C
Cuts at: 3716 3716
Size: 5537
NheI G'CTAG_C
Cuts at: 292 292
Size: 5537
NspI r_CATG'y
Cuts at: 2590 3090 3997 2590
Size: 500 907 4130
Fragments arranged by size:
4130 907 500
PasI CC'CwG_GG
Cuts at: 555 555
Size: 5537
PciI A'CATG_T
Cuts at: 3993 3993
Size: 5537
PflMI CCAn_nnn'nTGG
Cuts at: 1341 1659 1759 1341
Size: 318 100 5119
Fragments arranged by size:
5119 318 100
PfoI T'CCnGG_A
Cuts at: 1522 2282 2447 1522
Size: 760 165 4612
Fragments arranged by size:
4612 760 165
PmeI GTTT'AAAC
Cuts at: 2536 2536
Size: 5537
PmlI CAC'GTG
Cuts at: 1053 1053
Size: 5537
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4676 4708 4676
Size: 32 5505
PpuMI rG'GwC_Cy
Cuts at: 510 1900 510
Size: 1390 4147
PspOMI G'GGCC_C
Cuts at: 2147 2147
Size: 5537
PstI C_TGCA'G
Cuts at: 1943 2037 2136 2520 2584 3269 1943
Size: 94 99 384 64 685 4211
Fragments arranged by size:
4211 685 384 99 94 64
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5537
PvuII CAG'CTG
Cuts at: 1584 1971 2128 2214 2962 3322 1584
Size: 387 157 86 748 360 3799
Fragments arranged by size:
3799 748 387 360 157 86
RsrII CG'GwC_CG
Cuts at: 398 3732 398
Size: 3334 2203
SacI G_AGCT'C
Cuts at: 2551 2551
Size: 5537
SacII CC_GC'GG
Cuts at: 318 662 318
Size: 344 5193
SalI G'TCGA_C
Cuts at: 2574 2574
Size: 5537
SanDI GG'GwC_CC
Cuts at: 1900 1900
Size: 5537
SapI GCTCTTCn'nnn_
Cuts at: 1912 3560 3770 1912
Size: 1648 210 3679
Fragments arranged by size:
3679 1648 210
SbfI CC_TGCA'GG
Cuts at: 2584 2584
Size: 5537
ScaI AGT'ACT
Cuts at: 365 5054 365
Size: 4689 848
SexAI A'CCwGG_T
Cuts at: 1214 1214
Size: 5537
SfcI C'TryA_G
Cuts at: 33 1412 1849 1939 2033 2132 2516 2580 3265
Size: 1379 437 90 94 99 384 64 685
Cuts at: 3265 4258 4449 33
Size: 993 191 1121
Fragments arranged by size:
1379 1121 993 685 437 384 191 99
94 90 64
SfoI GGC'GCC
Cuts at: 2969 3217 2969
Size: 248 5289
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5537
SmaI CCC'GGG
Cuts at: 654 2559 654
Size: 1905 3632
SmlI C'TyrA_G
Cuts at: 2692 3607 4099 4361 2692
Size: 915 492 262 3868
Fragments arranged by size:
3868 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1531 67
Size: 1464 4073
SphI G_CATG'C
Cuts at: 2590 3090 2590
Size: 500 5037
SspI AAT'ATT
Cuts at: 1721 2082 1721
Size: 361 5176
StuI AGG'CCT
Cuts at: 1226 1226
Size: 5537
StyI C'CwwG_G
Cuts at: 107 1043 1271 2501 2671 3025 107
Size: 936 228 1230 170 354 2619
Fragments arranged by size:
2619 1230 936 354 228 170
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 375 759 1009 1666 1770 2401 3120 3253 3712
Size: 384 250 657 104 631 719 133 459
Cuts at: 3712 3921 375
Size: 209 1991
Fragments arranged by size:
1991 719 657 631 459 384 250 209
133 104
TatI w'GTAC_w
Cuts at: 363 1689 2274 5052 363
Size: 1326 585 2778 848
Fragments arranged by size:
2778 1326 848 585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 788 820 2256 2288 5054 5086 788
Size: 32 1436 32 2766 32 1239
Fragments arranged by size:
2766 1436 1239 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 274 3334 274
Size: 3060 2477
XbaI T'CTAG_A
Cuts at: 2568 2568
Size: 5537
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1005 2301 3032 1005
Size: 1296 731 3510
Fragments arranged by size:
3510 1296 731
XmaI C'CCGG_G
Cuts at: 652 2557 652
Size: 1905 3632
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BsiWI BsmI BsrGI BssHII BstZ17I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SfiI SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI