Restriction map for
pF1KB5752
(Circular) (Six-base) MAPSORT of: pF1KB5752.seq Check: 4824 from: 1 to: 5354
pF1KB5752 5354 bp
With 182 enzymes: SgfI *
June 12, 2017 09:50 ..
AatII G_ACGT'C
Cuts at: 2102 2102
Size: 5354
AccI GT'mk_AC
Cuts at: 962 2392 962
Size: 1430 3924
Acc65I G'GTAC_C
Cuts at: 530 2370 530
Size: 1840 3514
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 909 1739 1934 2111 3179 3611 4358 4626
Size: 791 830 195 177 1068 432 747 268
Cuts at: 4626 118
Size: 846
Fragments arranged by size:
1068 846 830 791 747 432 268 195
177
AflIII A'CryG_T
Cuts at: 2706 3810 4787 2706
Size: 1104 977 3273
Fragments arranged by size:
3273 1104 977
AgeI A'CCGG_T
Cuts at: 3742 3742
Size: 5354
AhdI GACnn_n'nnGTC
Cuts at: 927 927
Size: 5354
AlwNI CAG_nnn'CTG
Cuts at: 305 1957 2034 2089 2659 4226 305
Size: 1652 77 55 570 1567 1433
Fragments arranged by size:
1652 1567 1433 570 77 55
ApaI G_GGCC'C
Cuts at: 436 436
Size: 5354
ApaLI G'TGCA_C
Cuts at: 4124 4124
Size: 5354
ApoI r'AATT_y
Cuts at: 1090 1356 2197 2358 4707 4963 1090
Size: 266 841 161 2349 256 1481
Fragments arranged by size:
2349 1481 841 266 256 161
AseI AT'TA_AT
Cuts at: 20 782 827 1495 1499 1565 2288 20
Size: 762 45 668 4 66 723 3086
Fragments arranged by size:
3086 762 723 668 66 45 4
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 238 249 309 440 2374 238
Size: 11 60 131 1934 3218
Fragments arranged by size:
3218 1934 131 60 11
AvrII C'CTAG_G
Cuts at: 1294 1294
Size: 5354
BamHI G'GATC_C
Cuts at: 2379 2379
Size: 5354
BanI G'GyrC_C
Cuts at: 285 530 1298 2370 2784 3032 3067 285
Size: 245 768 1072 414 248 35 2572
Fragments arranged by size:
2572 1072 768 414 248 245 35
BanII G_rGCy'C
Cuts at: 250 260 436 2368 3398 250
Size: 10 176 1932 1030 2206
Fragments arranged by size:
2206 1932 1030 176 10
BbeI G_GCGC'C
Cuts at: 2788 3036 2788
Size: 248 5106
BbsI GAAGACnn'nnnn_
Cuts at: 482 1421 1721 482
Size: 939 300 4115
Fragments arranged by size:
4115 939 300
BbvCI CC'TCA_GC
Cuts at: 2072 2072
Size: 5354
BciVI GTATCCnnnnn_n'
Cuts at: 3252 4013 5354 3252
Size: 761 1341 3252
Fragments arranged by size:
3252 1341 761
BclI T'GATC_A
Cuts at: 2274 2874 2274
Size: 600 4754
BglI GCCn_nnn'nGGC
Cuts at: 431 431
Size: 5354
BglII A'GATC_T
Cuts at: 972 2869 4906 972
Size: 1897 2037 1420
Fragments arranged by size:
2037 1897 1420
BlpI GC'TnA_GC
Cuts at: 2466 2466
Size: 5354
Bme1580I G_kGCm'C
Cuts at: 436 1303 2979 3072 4128 436
Size: 867 1676 93 1056 1662
Fragments arranged by size:
1676 1662 1056 867 93
BmrI ACTGGGnnnn_n'
Cuts at: 1937 2566 2647 2982 5126 1937
Size: 629 81 335 2144 2165
Fragments arranged by size:
2165 2144 629 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3721 3721
Size: 5354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 908 2264 908
Size: 1356 3998
Bpu10I CC'TnA_GC
Cuts at: 2072 2072
Size: 5354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 971 1704 2530 3409 3901 4199 971
Size: 733 826 879 492 298 2126
Fragments arranged by size:
2126 879 826 733 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5058 5058
Size: 5354
BsaAI yAC'GTr
Cuts at: 2707 2707
Size: 5354
BsaBI GATnn'nnATC
Cuts at: 6 2024 2868 4905 6
Size: 2018 844 2037 455
Fragments arranged by size:
2037 2018 844 455
BsaHI Gr'CG_yC
Cuts at: 2099 2785 3033 3735 5231 2099
Size: 686 248 702 1496 2222
Fragments arranged by size:
2222 1496 702 686 248
BsaWI w'CCGG_w
Cuts at: 1899 2543 3064 3742 4016 4163 4574 1899
Size: 644 521 678 274 147 411 2679
Fragments arranged by size:
2679 678 644 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 166 196 213 243 723 753 881 911 166
Size: 30 17 30 480 30 128 30 4609
Fragments arranged by size:
4609 480 128 30 30 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 511 514 613 1274 1402 2261 511
Size: 3 99 661 128 859 3604
Fragments arranged by size:
3604 859 661 128 99 3
BseYI C'CCAG_C
Cuts at: 400 892 1302 2779 3537 4114 4610 400
Size: 492 410 1477 758 577 496 1144
Fragments arranged by size:
1477 1144 758 577 496 492 410
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4150 104
Size: 2792 46 1208 1308
Fragments arranged by size:
2792 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2368 3146 3336 4128 2368
Size: 778 190 792 3594
Fragments arranged by size:
3594 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 250 260 436 1303 2368 2979 3072 3146 3336
Size: 10 176 867 1065 611 93 74 190
Cuts at: 3336 3398 4128 250
Size: 62 730 1476
Fragments arranged by size:
1476 1065 867 730 611 190 176 93
74 62 10
BspEI T'CCGG_A
Cuts at: 1899 4574 1899
Size: 2675 2679
BspHI T'CATG_A
Cuts at: 1325 4530 1325
Size: 3205 2149
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2404 2618 2920 3301 2404
Size: 214 302 381 4457
Fragments arranged by size:
4457 381 302 214
BsrBI CCG'CTC
Cuts at: 50 204 243 3646 3700 5184 5349 50
Size: 154 39 3403 54 1484 165 55
Fragments arranged by size:
3403 1484 165 154 55 54 39
BsrDI GCAATG_nn'
Cuts at: 3266 3266
Size: 5354
BsrFI r'CCGG_y
Cuts at: 288 3352 3533 3742 288
Size: 3064 181 209 1900
Fragments arranged by size:
3064 1900 209 181
BssSI C'ACGA_G
Cuts at: 3625 3983 3625
Size: 358 4996
BstAPI GCAn_nnn'nTGC
Cuts at: 2731 2731
Size: 5354
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstYI r'GATC_y
Cuts at: 1 696 972 981 1020 2379 2846 2869 3204
Size: 695 276 9 39 1359 467 23 335
Cuts at: 3204 3450 4462 4548 4560 4906 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1359 1012 695 467 449 346 335 276
246 86 39 23 12 9
BtgI C'CryG_G
Cuts at: 107 217 646 2064 2171 107
Size: 110 429 1418 107 3290
Fragments arranged by size:
3290 1418 429 110 107
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1006 3304 3485 3648 1006
Size: 2298 181 163 2712
Fragments arranged by size:
2712 2298 181 163
BtsI GCAGTG_nn'
Cuts at: 2597 2597
Size: 5354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 731 766 731
Size: 35 5319
DraI TTT'AAA
Cuts at: 2353 2353
Size: 5354
DrdI GACnn_nn'nnGTC
Cuts at: 1555 3060 3918 1555
Size: 1505 858 2991
Fragments arranged by size:
2991 1505 858
EaeI y'GGCC_r
Cuts at: 296 455 2939 3113 3504 3531 4876 296
Size: 159 2484 174 391 27 1345 774
Fragments arranged by size:
2484 1345 774 391 174 159 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 500 1706 3377 3587 500
Size: 1206 1671 210 2267
Fragments arranged by size:
2267 1671 1206 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 358 3562 3872 4018 5164 127
Size: 231 3204 310 146 1146 317
Fragments arranged by size:
3204 1146 317 310 231 146
EcoICRI GAG'CTC
Cuts at: 2366 2366
Size: 5354
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 908 909 1739 1934 2111 2264 3179 3611
Size: 790 1 830 195 177 153 915 432
Cuts at: 3611 4358 4626 118
Size: 747 268 846
Fragments arranged by size:
915 846 830 790 747 432 268 195
177 153 1
EcoO109I rG'GnC_Cy
Cuts at: 432 2493 432
Size: 2061 3293
EcoRI G'AATT_C
Cuts at: 2197 2358 2197
Size: 161 5193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5354
FspI TGC'GCA
Cuts at: 3135 3135
Size: 5354
HaeII r_GCGC'y
Cuts at: 331 2072 2788 3036 4058 5037 331
Size: 1741 716 248 1022 979 648
Fragments arranged by size:
1741 1022 979 716 648 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 166 198 213 245 595 627 913 945 1881
Size: 32 15 32 350 32 286 32 936
Cuts at: 1881 1913 2089 2121 3196 3226 3228 3258 166
Size: 32 176 32 1075 30 2 30 2262
Fragments arranged by size:
2262 1075 936 350 286 176 32 32
32 32 32 32 30 30 15 2
HincII GTy'rAC
Cuts at: 1242 1407 2393 1242
Size: 165 986 4203
Fragments arranged by size:
4203 986 165
HindIII A'AGCT_T
Cuts at: 1624 1624
Size: 5354
KasI G'GCGC_C
Cuts at: 2784 3032 2784
Size: 248 5106
KpnI G_GTAC'C
Cuts at: 534 2374 534
Size: 1840 3514
MfeI C'AATT_G
Cuts at: 833 833
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 731 4025 4209 731
Size: 3294 184 1876
Fragments arranged by size:
3294 1876 184
MscI TGG'CCA
Cuts at: 3115 3115
Size: 5354
MslI CAynn'nnrTG
Cuts at: 584 1529 3343 3470 584
Size: 945 1814 127 2468
Fragments arranged by size:
2468 1814 945 127
MspA1I CmG'CkG
Cuts at: 219 453 1306 2465 2779 3139 4152 4397 219
Size: 234 853 1159 314 360 1013 245 1176
Fragments arranged by size:
1176 1159 1013 853 360 314 245 234
NaeI GCC'GGC
Cuts at: 290 3535 290
Size: 3245 2109
NarI GG'CG_CC
Cuts at: 2785 3033 2785
Size: 248 5106
NcoI C'CATG_G
Cuts at: 107 2064 2171 107
Size: 1957 107 3290
Fragments arranged by size:
3290 1957 107
NgoMIV G'CCGG_C
Cuts at: 288 3533 288
Size: 3245 2109
NspI r_CATG'y
Cuts at: 619 2147 2407 2907 3814 619
Size: 1528 260 500 907 2159
Fragments arranged by size:
2159 1528 907 500 260
PacI TTA_AT'TAA
Cuts at: 1499 1499
Size: 5354
PciI A'CATG_T
Cuts at: 3810 3810
Size: 5354
PfoI T'CCnGG_A
Cuts at: 1059 1059
Size: 5354
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4493 4525 4493
Size: 32 5322
PspOMI G'GGCC_C
Cuts at: 432 432
Size: 5354
PspXI vC'TCGA_Gb
Cuts at: 238 238
Size: 5354
PstI C_TGCA'G
Cuts at: 431 2401 3086 431
Size: 1970 685 2699
Fragments arranged by size:
2699 1970 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 1306 2779 3139 1306
Size: 1473 360 3521
Fragments arranged by size:
3521 1473 360
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 2368 2368
Size: 5354
SacII CC_GC'GG
Cuts at: 220 220
Size: 5354
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 3377 3587 3377
Size: 210 5144
SbfI CC_TGCA'GG
Cuts at: 431 2401 431
Size: 1970 3384
ScaI AGT'ACT
Cuts at: 1926 4871 1926
Size: 2945 2409
SfcI C'TryA_G
Cuts at: 33 427 959 2397 3082 4075 4266 33
Size: 394 532 1438 685 993 191 1121
Fragments arranged by size:
1438 1121 993 685 532 394 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 431 431
Size: 5354
SfoI GGC'GCC
Cuts at: 2786 3034 2786
Size: 248 5106
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 251 311 2376 251
Size: 60 2065 3229
Fragments arranged by size:
3229 2065 60
SmlI C'TyrA_G
Cuts at: 238 986 1719 2509 3424 3916 4178 238
Size: 748 733 790 915 492 262 1414
Fragments arranged by size:
1414 915 790 748 733 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 2407 2907 2407
Size: 500 4854
SrfI GCCC'GGGC
Cuts at: 251 251
Size: 5354
SspI AAT'ATT
Cuts at: 1009 1009
Size: 5354
StyI C'CwwG_G
Cuts at: 107 640 1294 2064 2171 2488 2842 107
Size: 533 654 770 107 317 354 2619
Fragments arranged by size:
2619 770 654 533 354 317 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1707 2937 3070 3529 3738 1707
Size: 1230 133 459 209 3323
Fragments arranged by size:
3323 1230 459 209 133
TatI w'GTAC_w
Cuts at: 1851 1924 4869 1851
Size: 73 2945 2336
Fragments arranged by size:
2945 2336 73
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 881 913 4871 4903 881
Size: 32 3958 32 1332
Fragments arranged by size:
3958 1332 32 32
Tth111I GACn'n_nGTC
Cuts at: 3151 3151
Size: 5354
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2849
Size: 5354
XhoI C'TCGA_G
Cuts at: 238 238
Size: 5354
XmaI C'CCGG_G
Cuts at: 249 309 2374 249
Size: 60 2065 3229
Fragments arranged by size:
3229 2065 60
XmnI GAAnn'nnTTC
Cuts at: 1370 1370
Size: 5354
ZraI GAC'GTC
Cuts at: 2100 2100
Size: 5354
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PacI
PciI PfoI PmeI PpiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
SrfI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AleI AlfI AloI AscI BaeI
BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI DraIII
EcoNI EcoRV FseI FspAI HpaI NdeI NheI NotI
NruI NsiI PasI PflMI PmlI PpuMI PshAI PsiI
PsrI SanDI SexAI SgrAI SnaBI StuI SwaI