Restriction map for
pF1KB5758
(Circular) (Six-base) MAPSORT of: pF1KB5758.seq Check: 6881 from: 1 to: 5330
pF1KB5758 5330 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 374 380 464 692 1864 2048 2368 374
Size: 6 84 228 1172 184 320 3336
Fragments arranged by size:
3336 1172 320 228 184 84 6
Acc65I G'GTAC_C
Cuts at: 2346 2346
Size: 5330
AclI AA'CG_TT
Cuts at: 5170 5170
Size: 5330
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 1760 1905 3155 3587 4334 4602 511
Size: 1249 145 1250 432 747 268 1239
Fragments arranged by size:
1250 1249 1239 747 432 268 145
AflIII A'CryG_T
Cuts at: 2682 3786 4763 2682
Size: 1104 977 3249
Fragments arranged by size:
3249 1104 977
AgeI A'CCGG_T
Cuts at: 3718 3718
Size: 5330
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 884 884
Size: 5330
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1868 1900 1868
Size: 32 5298
AlwNI CAG_nnn'CTG
Cuts at: 394 633 1006 2635 4202 394
Size: 239 373 1629 1567 1522
Fragments arranged by size:
1629 1567 1522 373 239
ApaLI G'TGCA_C
Cuts at: 4100 4100
Size: 5330
ApoI r'AATT_y
Cuts at: 697 1026 1089 1141 1723 2334 4683 4939 697
Size: 329 63 52 582 611 2349 256 1088
Fragments arranged by size:
2349 1088 611 582 329 256 63 52
AseI AT'TA_AT
Cuts at: 20 1310 20
Size: 1290 4040
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
AvaI C'yCGr_G
Cuts at: 2350 2350
Size: 5330
BamHI G'GATC_C
Cuts at: 2355 2355
Size: 5330
BanI G'GyrC_C
Cuts at: 300 2346 2760 3008 3043 300
Size: 2046 414 248 35 2587
Fragments arranged by size:
2587 2046 414 248 35
BanII G_rGCy'C
Cuts at: 401 2344 3374 401
Size: 1943 1030 2357
Fragments arranged by size:
2357 1943 1030
BbeI G_GCGC'C
Cuts at: 2764 3012 2764
Size: 248 5082
BbvCI CC'TCA_GC
Cuts at: 214 287 214
Size: 73 5257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 225 259 90
Size: 34 101 34 5161
Fragments arranged by size:
5161 101 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2285 3228 3989 5330 2285
Size: 943 761 1341 2285
Fragments arranged by size:
2285 1341 943 761
BclI T'GATC_A
Cuts at: 510 1127 1772 2850 510
Size: 617 645 1078 2990
Fragments arranged by size:
2990 1078 645 617
BglI GCCn_nnn'nGGC
Cuts at: 174 174
Size: 5330
BglII A'GATC_T
Cuts at: 333 1106 2201 2845 4882 333
Size: 773 1095 644 2037 781
Fragments arranged by size:
2037 1095 781 773 644
BlpI GC'TnA_GC
Cuts at: 395 2442 395
Size: 2047 3283
Bme1580I G_kGCm'C
Cuts at: 2955 3048 4104 2955
Size: 93 1056 4181
Fragments arranged by size:
4181 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1172 1598 2542 2623 2958 5102 1172
Size: 426 944 81 335 2144 1400
Fragments arranged by size:
2144 1400 944 426 335 81
BmtI G_CTAG'C
Cuts at: 316 316
Size: 5330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3697 3697
Size: 5330
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 621 655 914 992 1361 621
Size: 34 259 78 369 4590
Fragments arranged by size:
4590 369 259 78 34
Bpu10I CC'TnA_GC
Cuts at: 214 287 662 214
Size: 73 375 4882
Fragments arranged by size:
4882 375 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1014 2506 3385 3877 4175 1014
Size: 1492 879 492 298 2169
Fragments arranged by size:
2169 1492 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 990 5034 990
Size: 4044 1286
BsaAI yAC'GTr
Cuts at: 2683 2683
Size: 5330
BsaBI GATnn'nnATC
Cuts at: 6 2844 4881 6
Size: 2838 2037 455
Fragments arranged by size:
2838 2037 455
BsaHI Gr'CG_yC
Cuts at: 2761 3009 3711 5207 2761
Size: 248 702 1496 2884
Fragments arranged by size:
2884 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2519 3040 3718 3992 4139 4550 2519
Size: 521 678 274 147 411 3299
Fragments arranged by size:
3299 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 179 209 323 353 424 454 1277 1307 2257
Size: 30 114 30 71 30 823 30 950
Cuts at: 2257 2287 179
Size: 30 3222
Fragments arranged by size:
3222 950 823 114 71 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 304 510 304
Size: 206 5124
BseYI C'CCAG_C
Cuts at: 839 2215 2755 3513 4090 4586 839
Size: 1376 540 758 577 496 1583
Fragments arranged by size:
1583 1376 758 577 540 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 992
Size: 5330
BsiEI CG_ry'CG
Cuts at: 104 2872 2918 4126 104
Size: 2768 46 1208 1308
Fragments arranged by size:
2768 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 144 401 1166 2344 3122 3312 4104 144
Size: 257 765 1178 778 190 792 1370
Fragments arranged by size:
1370 1178 792 778 765 257 190
Bsp1286I G_dGCh'C
Cuts at: 144 401 1166 2344 2955 3048 3122 3312 3374
Size: 257 765 1178 611 93 74 190 62
Cuts at: 3374 4104 144
Size: 730 1370
Fragments arranged by size:
1370 1178 765 730 611 257 190 93
74 62
BspEI T'CCGG_A
Cuts at: 4550 4550
Size: 5330
BspHI T'CATG_A
Cuts at: 4506 4506
Size: 5330
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2380 2594 2896 3277 2380
Size: 214 302 381 4433
Fragments arranged by size:
4433 381 302 214
BsrBI CCG'CTC
Cuts at: 50 151 1267 3622 3676 5160 5325 50
Size: 101 1116 2355 54 1484 165 55
Fragments arranged by size:
2355 1484 1116 165 101 55 54
BsrDI GCAATG_nn'
Cuts at: 3242 3242
Size: 5330
BsrFI r'CCGG_y
Cuts at: 206 3328 3509 3718 206
Size: 3122 181 209 1818
Fragments arranged by size:
3122 1818 209 181
BssSI C'ACGA_G
Cuts at: 1526 3601 3959 1526
Size: 2075 358 2897
Fragments arranged by size:
2897 2075 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2707 2707
Size: 5330
BstBI TT'CG_AA
Cuts at: 3691 3691
Size: 5330
BstYI r'GATC_y
Cuts at: 1 333 629 873 1106 1173 2201 2355 2822
Size: 332 296 244 233 67 1028 154 467
Cuts at: 2822 2845 3180 3426 4438 4524 4536 4882 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1028 1012 467 449 346 335 332 296
246 244 233 154 86 67 23 12
BstZ17I GTA'TAC
Cuts at: 1865 1865
Size: 5330
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3280 3461 3624 3280
Size: 181 163 4986
Fragments arranged by size:
4986 181 163
BtsI GCAGTG_nn'
Cuts at: 323 395 2153 2573 323
Size: 72 1758 420 3080
Fragments arranged by size:
3080 1758 420 72
ClaI AT'CG_AT
Cuts at: 641 641
Size: 5330
DraI TTT'AAA
Cuts at: 674 2329 674
Size: 1655 3675
DrdI GACnn_nn'nnGTC
Cuts at: 3036 3894 3036
Size: 858 4472
EaeI y'GGCC_r
Cuts at: 684 1338 2915 3089 3480 3507 4852 684
Size: 654 1577 174 391 27 1345 1162
Fragments arranged by size:
1577 1345 1162 654 391 174 27
EagI C'GGCC_G
Cuts at: 2915 2915
Size: 5330
EarI CTCTTCn'nnn_
Cuts at: 487 1777 3353 3563 487
Size: 1290 1576 210 2254
Fragments arranged by size:
2254 1576 1290 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 193 3538 3848 3994 5140 193
Size: 3345 310 146 1146 383
Fragments arranged by size:
3345 1146 383 310 146
EcoICRI GAG'CTC
Cuts at: 399 2342 399
Size: 1943 3387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 511 621 655 914 992 1361 1760 1905 3155
Size: 110 34 259 78 369 399 145 1250
Cuts at: 3155 3587 4334 4602 511
Size: 432 747 268 1239
Fragments arranged by size:
1250 1239 747 432 399 369 268 259
145 110 78 34
EcoNI CCTnn'n_nnAGG
Cuts at: 1360 1360
Size: 5330
EcoO109I rG'GnC_Cy
Cuts at: 2469 2469
Size: 5330
EcoRI G'AATT_C
Cuts at: 1141 1723 2334 1141
Size: 582 611 4137
Fragments arranged by size:
4137 611 582
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1561 3349 1561
Size: 1788 3542
FspI TGC'GCA
Cuts at: 3111 3111
Size: 5330
HaeII r_GCGC'y
Cuts at: 2079 2764 3012 4034 5013 2079
Size: 685 248 1022 979 2396
Fragments arranged by size:
2396 1022 979 685 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 179 211 1098 1130 1435 1467 1644 1676 2041
Size: 32 887 32 305 32 177 32 365
Cuts at: 2041 2073 2161 2186 2193 2218 3172 3202 3204
Size: 32 88 25 7 25 954 30 2
Cuts at: 3204 3234 179
Size: 30 2275
Fragments arranged by size:
2275 954 887 365 305 177 88 32
32 32 32 32 30 30 25 25
7 2
HincII GTy'rAC
Cuts at: 1760 2369 1760
Size: 609 4721
HindIII A'AGCT_T
Cuts at: 515 515
Size: 5330
HpaI GTT'AAC
Cuts at: 1760 1760
Size: 5330
KasI G'GCGC_C
Cuts at: 2760 3008 2760
Size: 248 5082
KpnI G_GTAC'C
Cuts at: 2350 2350
Size: 5330
MfeI C'AATT_G
Cuts at: 1616 1730 1616
Size: 114 5216
MluI A'CGCG_T
Cuts at: 4763 4763
Size: 5330
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1347 1450 1464 4001 4185 1347
Size: 103 14 2537 184 2492
Fragments arranged by size:
2537 2492 184 103 14
MscI TGG'CCA
Cuts at: 686 3091 686
Size: 2405 2925
MslI CAynn'nnrTG
Cuts at: 1950 3319 3446 1950
Size: 1369 127 3834
Fragments arranged by size:
3834 1369 127
MspA1I CmG'CkG
Cuts at: 1256 2441 2755 3115 4128 4373 1256
Size: 1185 314 360 1013 245 2213
Fragments arranged by size:
2213 1185 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3511 3511
Size: 5330
NarI GG'CG_CC
Cuts at: 2761 3009 2761
Size: 248 5082
NgoMIV G'CCGG_C
Cuts at: 3509 3509
Size: 5330
NheI G'CTAG_C
Cuts at: 312 312
Size: 5330
NspI r_CATG'y
Cuts at: 984 2383 2883 3790 984
Size: 1399 500 907 2524
Fragments arranged by size:
2524 1399 907 500
PciI A'CATG_T
Cuts at: 3786 3786
Size: 5330
PflMI CCAn_nnn'nTGG
Cuts at: 1209 1209
Size: 5330
PfoI T'CCnGG_A
Cuts at: 259 632 259
Size: 373 4957
PmeI GTTT'AAAC
Cuts at: 2329 2329
Size: 5330
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 4469
Size: 32 5298
PstI C_TGCA'G
Cuts at: 2377 3062 2377
Size: 685 4645
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5330
PvuII CAG'CTG
Cuts at: 2755 3115 2755
Size: 360 4970
RsrII CG'GwC_CG
Cuts at: 3525 3525
Size: 5330
SacI G_AGCT'C
Cuts at: 401 2344 401
Size: 1943 3387
SalI G'TCGA_C
Cuts at: 2367 2367
Size: 5330
SapI GCTCTTCn'nnn_
Cuts at: 3353 3563 3353
Size: 210 5120
SbfI CC_TGCA'GG
Cuts at: 2377 2377
Size: 5330
ScaI AGT'ACT
Cuts at: 4847 4847
Size: 5330
SfcI C'TryA_G
Cuts at: 33 1934 2373 3058 4051 4242 33
Size: 1901 439 685 993 191 1121
Fragments arranged by size:
1901 1121 993 685 439 191
SfoI GGC'GCC
Cuts at: 2762 3010 2762
Size: 248 5082
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5330
SmaI CCC'GGG
Cuts at: 2352 2352
Size: 5330
SmlI C'TyrA_G
Cuts at: 993 2485 3400 3892 4154 993
Size: 1492 915 492 262 2169
Fragments arranged by size:
2169 1492 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5330
SphI G_CATG'C
Cuts at: 2383 2883 2383
Size: 500 4830
SspI AAT'ATT
Cuts at: 588 588
Size: 5330
StyI C'CwwG_G
Cuts at: 1053 2464 2818 1053
Size: 1411 354 3565
Fragments arranged by size:
3565 1411 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 482 2913 3046 3505 3714 482
Size: 2431 133 459 209 2098
Fragments arranged by size:
2431 2098 459 209 133
TatI w'GTAC_w
Cuts at: 4845 4845
Size: 5330
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 323 355 4847 4879 323
Size: 32 4492 32 774
Fragments arranged by size:
4492 774 32 32
Tth111I GACn'n_nGTC
Cuts at: 3127 3127
Size: 5330
XbaI T'CTAG_A
Cuts at: 2361 2361
Size: 5330
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2825 2825
Size: 5330
XmaI C'CCGG_G
Cuts at: 2350 2350
Size: 5330
XmnI GAAnn'nnTTC
Cuts at: 740 1786 1817 740
Size: 1046 31 4253
Fragments arranged by size:
4253 1046 31
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NspI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI ApaI AscI
AvrII BaeI BbsI BfrBI BmgBI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI Bsu36I BtgI CspCI DraIII
EcoRV FseI FspAI NcoI NdeI NotI NruI NsiI
PacI PasI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI