Restriction map for
pF1KB5763
(Circular) (Six-base) MAPSORT of: pF1KB5763.seq Check: 3653 from: 1 to: 5561
pF1KB5763 5561 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 336 336
Size: 5561
AccI GT'mk_AC
Cuts at: 506 823 2599 506
Size: 317 1776 3468
Fragments arranged by size:
3468 1776 317
Acc65I G'GTAC_C
Cuts at: 785 943 2577 785
Size: 158 1634 3769
Fragments arranged by size:
3769 1634 158
AclI AA'CG_TT
Cuts at: 5401 5401
Size: 5561
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 436 637 676 3386 3818 4565 4833 142
Size: 294 201 39 2710 432 747 268 870
Fragments arranged by size:
2710 870 747 432 294 268 201 39
AfeI AGC'GCT
Cuts at: 1107 1107
Size: 5561
AflIII A'CryG_T
Cuts at: 579 2913 4017 4994 579
Size: 2334 1104 977 1146
Fragments arranged by size:
2334 1146 1104 977
AgeI A'CCGG_T
Cuts at: 3949 3949
Size: 5561
AhdI GACnn_n'nnGTC
Cuts at: 843 843
Size: 5561
AleI CACnn'nnGTG
Cuts at: 1861 1861
Size: 5561
AlwNI CAG_nnn'CTG
Cuts at: 1123 1126 1373 2866 4433 1123
Size: 3 247 1493 1567 2251
Fragments arranged by size:
2251 1567 1493 247 3
ApaI G_GGCC'C
Cuts at: 167 1759 2051 167
Size: 1592 292 3677
Fragments arranged by size:
3677 1592 292
ApaLI G'TGCA_C
Cuts at: 729 4331 729
Size: 3602 1959
ApoI r'AATT_y
Cuts at: 493 2353 2565 4914 5170 493
Size: 1860 212 2349 256 884
Fragments arranged by size:
2349 1860 884 256 212
AseI AT'TA_AT
Cuts at: 20 20
Size: 5561
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5561
AvaI C'yCGr_G
Cuts at: 160 796 1226 2581 160
Size: 636 430 1355 3140
Fragments arranged by size:
3140 1355 636 430
BamHI G'GATC_C
Cuts at: 2586 2586
Size: 5561
BanI G'GyrC_C
Cuts at: 785 881 943 1051 1513 2577 2991 3239 3274
Size: 96 62 108 462 1064 414 248 35
Cuts at: 3274 785
Size: 3072
Fragments arranged by size:
3072 1064 462 414 248 108 96 62
35
BanII G_rGCy'C
Cuts at: 158 167 695 803 1103 1380 1759 1975 2051
Size: 9 528 108 300 277 379 216 76
Cuts at: 2051 2205 2575 3605 158
Size: 154 370 1030 2114
Fragments arranged by size:
2114 1030 528 379 370 300 277 216
154 108 76 9
BbeI G_GCGC'C
Cuts at: 885 2995 3243 885
Size: 2110 248 3203
Fragments arranged by size:
3203 2110 248
BbsI GAAGACnn'nnnn_
Cuts at: 137 2204 137
Size: 2067 3494
BciVI GTATCCnnnnn_n'
Cuts at: 3459 4220 5561 3459
Size: 761 1341 3459
Fragments arranged by size:
3459 1341 761
BclI T'GATC_A
Cuts at: 3081 3081
Size: 5561
BglI GCCn_nnn'nGGC
Cuts at: 162 338 1252 162
Size: 176 914 4471
Fragments arranged by size:
4471 914 176
BglII A'GATC_T
Cuts at: 3076 5113 3076
Size: 2037 3524
BlpI GC'TnA_GC
Cuts at: 2673 2673
Size: 5561
Bme1580I G_kGCm'C
Cuts at: 167 485 733 1031 1188 1759 2051 3186 3279
Size: 318 248 298 157 571 292 1135 93
Cuts at: 3279 4335 167
Size: 1056 1393
Fragments arranged by size:
1393 1135 1056 571 318 298 292 248
157 93
BmgBI CAC'GTC
Cuts at: 258 258
Size: 5561
BmrI ACTGGGnnnn_n'
Cuts at: 1361 1376 1897 2100 2773 2854 3189 5333 1361
Size: 15 521 203 673 81 335 2144 1589
Fragments arranged by size:
2144 1589 673 521 335 203 81 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 162 3928 162
Size: 3766 1795
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1624 2124 2368 2530 1624
Size: 500 244 162 4655
Fragments arranged by size:
4655 500 244 162
Bpu10I CC'TnA_GC
Cuts at: 635 1613 635
Size: 978 4583
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 409 679 1543 2737 3616 4108 4406 409
Size: 270 864 1194 879 492 298 1564
Fragments arranged by size:
1564 1194 879 864 492 298 270
BsaI GGTCTCn'nnnn_
Cuts at: 277 5265 277
Size: 4988 573
BsaAI yAC'GTr
Cuts at: 510 582 2914 510
Size: 72 2332 3157
Fragments arranged by size:
3157 2332 72
BsaBI GATnn'nnATC
Cuts at: 6 3075 5112 6
Size: 3069 2037 455
Fragments arranged by size:
3069 2037 455
BsaHI Gr'CG_yC
Cuts at: 882 2192 2992 3240 3942 5438 882
Size: 1310 800 248 702 1496 1005
Fragments arranged by size:
1496 1310 1005 800 702 248
BsaWI w'CCGG_w
Cuts at: 2750 3271 3949 4223 4370 4781 2750
Size: 521 678 274 147 411 3530
Fragments arranged by size:
3530 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1027 1057 1905 1935 2025 2055 1027
Size: 30 848 30 90 30 4533
Fragments arranged by size:
4533 848 90 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 546 1584 1675 1913 546
Size: 1038 91 238 4194
Fragments arranged by size:
4194 1038 238 91
BseYI C'CCAG_C
Cuts at: 939 1077 1102 1591 1741 1848 1878 1940 1994
Size: 138 25 489 150 107 30 62 54
Cuts at: 1994 2249 2986 3744 4321 4817 939
Size: 255 737 758 577 496 1683
Fragments arranged by size:
1683 758 737 577 496 489 255 150
138 107 62 54 30 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 523 1424 2006 523
Size: 901 582 4078
Fragments arranged by size:
4078 901 582
BsiEI CG_ry'CG
Cuts at: 104 1059 3103 3149 4357 104
Size: 955 2044 46 1208 1308
Fragments arranged by size:
2044 1308 1208 955 46
BsiHKAI G_wGCw'C
Cuts at: 695 733 803 1790 2575 3353 3543 4335 695
Size: 38 70 987 785 778 190 792 1921
Fragments arranged by size:
1921 987 792 785 778 190 70 38
Bsp1286I G_dGCh'C
Cuts at: 158 167 485 695 733 803 1031 1103 1188
Size: 9 318 210 38 70 228 72 85
Cuts at: 1188 1380 1759 1790 1975 2051 2205 2575 3186
Size: 192 379 31 185 76 154 370 611
Cuts at: 3186 3279 3353 3543 3605 4335 158
Size: 93 74 190 62 730 1384
Fragments arranged by size:
1384 730 611 379 370 318 228 210
192 190 185 154 93 85 76 74
72 70 62 38 31 9
BspEI T'CCGG_A
Cuts at: 4781 4781
Size: 5561
BspHI T'CATG_A
Cuts at: 758 929 4737 758
Size: 171 3808 1582
Fragments arranged by size:
3808 1582 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 545 2543 2611 2825 3127 3508 336
Size: 209 1998 68 214 302 381 2389
Fragments arranged by size:
2389 1998 381 302 214 209 68
BsrBI CCG'CTC
Cuts at: 50 236 1656 1698 1719 3853 3907 5391 5556
Size: 186 1420 42 21 2134 54 1484 165
Cuts at: 5556 50
Size: 55
Fragments arranged by size:
2134 1484 1420 186 165 55 54 42
21
BsrDI GCAATG_nn'
Cuts at: 3473 3473
Size: 5561
BsrFI r'CCGG_y
Cuts at: 337 1574 1626 1866 3559 3740 3949 337
Size: 1237 52 240 1693 181 209 1949
Fragments arranged by size:
1949 1693 1237 240 209 181 52
BsrGI T'GTAC_A
Cuts at: 909 909
Size: 5561
BssSI C'ACGA_G
Cuts at: 719 3832 4190 719
Size: 3113 358 2090
Fragments arranged by size:
3113 2090 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1126 2938 1126
Size: 1812 3749
BstBI TT'CG_AA
Cuts at: 3922 3922
Size: 5561
BstEII G'GTnAC_C
Cuts at: 384 384
Size: 5561
BstXI CCAn_nnnn'nTGG
Cuts at: 216 672 1948 2035 2097 216
Size: 456 1276 87 62 3680
Fragments arranged by size:
3680 1276 456 87 62
BstYI r'GATC_y
Cuts at: 1 362 419 1301 2501 2586 3053 3076 3411
Size: 361 57 882 1200 85 467 23 335
Cuts at: 3411 3657 4669 4755 4767 5113 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1200 1012 882 467 449 361 346 335
246 86 85 57 23 12
BstZ17I GTA'TAC
Cuts at: 824 824
Size: 5561
Bsu36I CC'TnA_GG
Cuts at: 406 469 406
Size: 63 5498
BtgI C'CryG_G
Cuts at: 107 1397 2460 107
Size: 1290 1063 3208
Fragments arranged by size:
3208 1290 1063
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 435 3511 3692 3855 435
Size: 3076 181 163 2141
Fragments arranged by size:
3076 2141 181 163
BtsI GCAGTG_nn'
Cuts at: 1072 2322 2804 1072
Size: 1250 482 3829
Fragments arranged by size:
3829 1250 482
DraI TTT'AAA
Cuts at: 435 2560 435
Size: 2125 3436
DrdI GACnn_nn'nnGTC
Cuts at: 3267 4125 3267
Size: 858 4703
EaeI y'GGCC_r
Cuts at: 110 206 339 350 773 1148 2403 3146 3320
Size: 96 133 11 423 375 1255 743 174
Cuts at: 3320 3711 3738 5083 110
Size: 391 27 1345 588
Fragments arranged by size:
1345 1255 743 588 423 391 375 174
133 96 27 11
EagI C'GGCC_G
Cuts at: 3146 3146
Size: 5561
EarI CTCTTCn'nnn_
Cuts at: 895 3584 3794 895
Size: 2689 210 2662
Fragments arranged by size:
2689 2662 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 309 1282 1686 3769 4079 4225 5371 309
Size: 973 404 2083 310 146 1146 499
Fragments arranged by size:
2083 1146 973 499 404 310 146
EcoICRI GAG'CTC
Cuts at: 693 801 2573 693
Size: 108 1772 3681
Fragments arranged by size:
3681 1772 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 142 436 637 676 1624 2124 2368 2530 3386
Size: 294 201 39 948 500 244 162 856
Cuts at: 3386 3818 4565 4833 142
Size: 432 747 268 870
Fragments arranged by size:
948 870 856 747 500 432 294 268
244 201 162 39
EcoO109I rG'GnC_Cy
Cuts at: 299 612 1065 1745 1755 1756 2277 2550 2700
Size: 313 453 680 10 1 521 273 150
Cuts at: 2700 299
Size: 3160
Fragments arranged by size:
3160 680 521 453 313 273 150 10
1
EcoRI G'AATT_C
Cuts at: 493 2353 2565 493
Size: 1860 212 3489
Fragments arranged by size:
3489 1860 212
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 5561
FspI TGC'GCA
Cuts at: 3342 3342
Size: 5561
HaeII r_GCGC'y
Cuts at: 885 1109 1647 1885 2995 3243 4265 5244 885
Size: 224 538 238 1110 248 1022 979 1202
Fragments arranged by size:
1202 1110 1022 979 538 248 238 224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 829 861 1772 1804 2404 2436 2470 2502 3403
Size: 32 911 32 600 32 34 32 901
Cuts at: 3403 3433 3435 3465 829
Size: 30 2 30 2925
Fragments arranged by size:
2925 911 901 600 34 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2238 2600 2238
Size: 362 5199
KasI G'GCGC_C
Cuts at: 881 2991 3239 881
Size: 2110 248 3203
Fragments arranged by size:
3203 2110 248
KpnI G_GTAC'C
Cuts at: 789 947 2581 789
Size: 158 1634 3769
Fragments arranged by size:
3769 1634 158
MluI A'CGCG_T
Cuts at: 4994 4994
Size: 5561
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 689 1793 1932 4232 4416 689
Size: 1104 139 2300 184 1834
Fragments arranged by size:
2300 1834 1104 184 139
MscI TGG'CCA
Cuts at: 352 775 1150 2405 3322 352
Size: 423 375 1255 917 2591
Fragments arranged by size:
2591 1255 917 423 375
MslI CAynn'nnrTG
Cuts at: 931 934 1861 2380 3550 3677 931
Size: 3 927 519 1170 127 2815
Fragments arranged by size:
2815 1170 927 519 127 3
MspA1I CmG'CkG
Cuts at: 193 742 1123 1650 2672 2986 3346 4359 4604
Size: 549 381 527 1022 314 360 1013 245
Cuts at: 4604 193
Size: 1150
Fragments arranged by size:
1150 1022 1013 549 527 381 360 314
245
NaeI GCC'GGC
Cuts at: 1576 1868 3742 1576
Size: 292 1874 3395
Fragments arranged by size:
3395 1874 292
NarI GG'CG_CC
Cuts at: 882 2992 3240 882
Size: 2110 248 3203
Fragments arranged by size:
3203 2110 248
NcoI C'CATG_G
Cuts at: 107 1397 2460 107
Size: 1290 1063 3208
Fragments arranged by size:
3208 1290 1063
NgoMIV G'CCGG_C
Cuts at: 1574 1866 3740 1574
Size: 292 1874 3395
Fragments arranged by size:
3395 1874 292
NspI r_CATG'y
Cuts at: 627 1089 2614 3114 4021 627
Size: 462 1525 500 907 2167
Fragments arranged by size:
2167 1525 907 500 462
PasI CC'CwG_GG
Cuts at: 1031 1373 2125 1031
Size: 342 752 4467
Fragments arranged by size:
4467 752 342
PciI A'CATG_T
Cuts at: 4017 4017
Size: 5561
PflMI CCAn_nnn'nTGG
Cuts at: 348 782 1373 2014 2125 2401 348
Size: 434 591 641 111 276 3508
Fragments arranged by size:
3508 641 591 434 276 111
PfoI T'CCnGG_A
Cuts at: 515 2546 515
Size: 2031 3530
PmeI GTTT'AAAC
Cuts at: 2560 2560
Size: 5561
PmlI CAC'GTG
Cuts at: 582 582
Size: 5561
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4700 4732 4700
Size: 32 5529
PpuMI rG'GwC_Cy
Cuts at: 2277 2550 2277
Size: 273 5288
PspOMI G'GGCC_C
Cuts at: 163 1755 2047 163
Size: 1592 292 3677
Fragments arranged by size:
3677 1592 292
PstI C_TGCA'G
Cuts at: 1469 2495 2608 3293 1469
Size: 1026 113 685 3737
Fragments arranged by size:
3737 1026 685 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5561
PvuII CAG'CTG
Cuts at: 1123 2986 3346 1123
Size: 1863 360 3338
Fragments arranged by size:
3338 1863 360
RsrII CG'GwC_CG
Cuts at: 3756 3756
Size: 5561
SacI G_AGCT'C
Cuts at: 695 803 2575 695
Size: 108 1772 3681
Fragments arranged by size:
3681 1772 108
SalI G'TCGA_C
Cuts at: 2598 2598
Size: 5561
SapI GCTCTTCn'nnn_
Cuts at: 895 3584 3794 895
Size: 2689 210 2662
Fragments arranged by size:
2689 2662 210
SbfI CC_TGCA'GG
Cuts at: 2608 2608
Size: 5561
ScaI AGT'ACT
Cuts at: 754 5078 754
Size: 4324 1237
SfcI C'TryA_G
Cuts at: 33 200 919 1321 1465 1934 2491 2604 3289
Size: 167 719 402 144 469 557 113 685
Cuts at: 3289 4282 4473 33
Size: 993 191 1121
Fragments arranged by size:
1121 993 719 685 557 469 402 191
167 144 113
SfoI GGC'GCC
Cuts at: 883 2993 3241 883
Size: 2110 248 3203
Fragments arranged by size:
3203 2110 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5561
SmaI CCC'GGG
Cuts at: 162 2583 162
Size: 2421 3140
SmlI C'TyrA_G
Cuts at: 424 694 1558 2716 3631 4123 4385 424
Size: 270 864 1158 915 492 262 1600
Fragments arranged by size:
1600 1158 915 864 492 270 262
SpeI A'CTAG_T
Cuts at: 67 1782 67
Size: 1715 3846
SphI G_CATG'C
Cuts at: 1089 2614 3114 1089
Size: 1525 500 3536
Fragments arranged by size:
3536 1525 500
StyI C'CwwG_G
Cuts at: 107 1397 1691 1772 2460 2695 3049 107
Size: 1290 294 81 688 235 354 2619
Fragments arranged by size:
2619 1290 688 354 294 235 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3144 3277 3736 3945 3144
Size: 133 459 209 4760
Fragments arranged by size:
4760 459 209 133
TatI w'GTAC_w
Cuts at: 620 752 909 5076 620
Size: 132 157 4167 1105
Fragments arranged by size:
4167 1105 157 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2025 2057 5078 5110 2025
Size: 32 3021 32 2476
Fragments arranged by size:
3021 2476 32 32
Tth111I GACn'n_nGTC
Cuts at: 3358 3358
Size: 5561
XbaI T'CTAG_A
Cuts at: 2592 2592
Size: 5561
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1599 1770 2094 3056 1599
Size: 171 324 962 4104
Fragments arranged by size:
4104 962 324 171
XmaI C'CCGG_G
Cuts at: 160 2581 160
Size: 2421 3140
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AlfI AloI AscI AvrII BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmI BsmBI BssHII ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PshAI
PsiI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI