Restriction map for
pF1KB5764
(Circular) (Six-base) MAPSORT of: pF1KB5764.seq Check: 4566 from: 1 to: 5381
pF1KB5764 5381 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 2191 2419 2191
Size: 228 5153
Acc65I G'GTAC_C
Cuts at: 2397 2397
Size: 5381
AclI AA'CG_TT
Cuts at: 5221 5221
Size: 5381
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1442 1457 1972 3206 3638 4385 4653 295
Size: 1147 15 515 1234 432 747 268 1023
Fragments arranged by size:
1234 1147 1023 747 515 432 268 15
AflIII A'CryG_T
Cuts at: 2733 3837 4814 2733
Size: 1104 977 3300
Fragments arranged by size:
3300 1104 977
AgeI A'CCGG_T
Cuts at: 3769 3769
Size: 5381
AleI CACnn'nnGTG
Cuts at: 2095 2095
Size: 5381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1649 1681 1649
Size: 32 5349
AlwNI CAG_nnn'CTG
Cuts at: 712 1305 1417 2686 4253 712
Size: 593 112 1269 1567 1840
Fragments arranged by size:
1840 1567 1269 593 112
ApaLI G'TGCA_C
Cuts at: 1027 4151 1027
Size: 3124 2257
ApoI r'AATT_y
Cuts at: 632 2385 4734 4990 632
Size: 1753 2349 256 1023
Fragments arranged by size:
2349 1753 1023 256
AseI AT'TA_AT
Cuts at: 20 1646 20
Size: 1626 3755
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
AvaI C'yCGr_G
Cuts at: 1271 2401 1271
Size: 1130 4251
BamHI G'GATC_C
Cuts at: 2406 2406
Size: 5381
BanI G'GyrC_C
Cuts at: 301 2397 2811 3059 3094 301
Size: 2096 414 248 35 2588
Fragments arranged by size:
2588 2096 414 248 35
BanII G_rGCy'C
Cuts at: 1117 2395 3425 1117
Size: 1278 1030 3073
Fragments arranged by size:
3073 1278 1030
BbeI G_GCGC'C
Cuts at: 2815 3063 2815
Size: 248 5133
BbsI GAAGACnn'nnnn_
Cuts at: 1205 1205
Size: 5381
BciVI GTATCCnnnnn_n'
Cuts at: 1449 3279 4040 5381 1449
Size: 1830 761 1341 1449
Fragments arranged by size:
1830 1449 1341 761
BclI T'GATC_A
Cuts at: 2901 2901
Size: 5381
BfrBI ATG'CAT
Cuts at: 1291 1291
Size: 5381
BglII A'GATC_T
Cuts at: 321 2896 4933 321
Size: 2575 2037 769
Fragments arranged by size:
2575 2037 769
BlpI GC'TnA_GC
Cuts at: 2493 2493
Size: 5381
Bme1580I G_kGCm'C
Cuts at: 1031 2051 3006 3099 4155 1031
Size: 1020 955 93 1056 2257
Fragments arranged by size:
2257 1056 1020 955 93
BmrI ACTGGGnnnn_n'
Cuts at: 2593 2674 3009 5153 2593
Size: 81 335 2144 2821
Fragments arranged by size:
2821 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3748 3748
Size: 5381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1096 1110 1618 1096
Size: 14 508 4859
Fragments arranged by size:
4859 508 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 942 2557 3436 3928 4226 942
Size: 1615 879 492 298 2097
Fragments arranged by size:
2097 1615 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 5085 5085
Size: 5381
BsaAI yAC'GTr
Cuts at: 2734 2734
Size: 5381
BsaBI GATnn'nnATC
Cuts at: 6 286 2895 4932 6
Size: 280 2609 2037 455
Fragments arranged by size:
2609 2037 455 280
BsaHI Gr'CG_yC
Cuts at: 2812 3060 3762 5258 2812
Size: 248 702 1496 2935
Fragments arranged by size:
2935 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2165 2570 3091 3769 4043 4190 4601 2165
Size: 405 521 678 274 147 411 2945
Fragments arranged by size:
2945 678 521 411 405 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 692 722 692
Size: 30 5351
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 606 1078 1408 2200 606
Size: 472 330 792 3787
Fragments arranged by size:
3787 792 472 330
BseYI C'CCAG_C
Cuts at: 1410 2806 3564 4141 4637 1410
Size: 1396 758 577 496 2154
Fragments arranged by size:
2154 1396 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1042 1105 1042
Size: 63 5318
BsiEI CG_ry'CG
Cuts at: 104 2923 2969 4177 104
Size: 2819 46 1208 1308
Fragments arranged by size:
2819 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1031 2395 3173 3363 4155 1031
Size: 1364 778 190 792 2257
Fragments arranged by size:
2257 1364 792 778 190
BsmI GAATG_Cn'
Cuts at: 2149 2149
Size: 5381
Bsp1286I G_dGCh'C
Cuts at: 1031 1117 2051 2395 3006 3099 3173 3363 3425
Size: 86 934 344 611 93 74 190 62
Cuts at: 3425 4155 1031
Size: 730 2257
Fragments arranged by size:
2257 934 730 611 344 190 93 86
74 62
BspEI T'CCGG_A
Cuts at: 2165 4601 2165
Size: 2436 2945
BspHI T'CATG_A
Cuts at: 4557 4557
Size: 5381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 641 2351 2431 2645 2947 3328 641
Size: 1710 80 214 302 381 2694
Fragments arranged by size:
2694 1710 381 302 214 80
BsrBI CCG'CTC
Cuts at: 50 1080 1851 3673 3727 5211 5376 50
Size: 1030 771 1822 54 1484 165 55
Fragments arranged by size:
1822 1484 1030 771 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3293 3293
Size: 5381
BsrFI r'CCGG_y
Cuts at: 3379 3560 3769 3379
Size: 181 209 4991
Fragments arranged by size:
4991 209 181
BssSI C'ACGA_G
Cuts at: 588 1060 3652 4010 588
Size: 472 2592 358 1959
Fragments arranged by size:
2592 1959 472 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2758 2758
Size: 5381
BstBI TT'CG_AA
Cuts at: 3742 3742
Size: 5381
BstXI CCAn_nnnn'nTGG
Cuts at: 298 2240 298
Size: 1942 3439
BstYI r'GATC_y
Cuts at: 1 287 321 2406 2873 2896 3231 3477 4489
Size: 286 34 2085 467 23 335 246 1012
Cuts at: 4489 4575 4587 4933 1
Size: 86 12 346 449
Fragments arranged by size:
2085 1012 467 449 346 335 286 246
86 34 23 12
BtgI C'CryG_G
Cuts at: 743 743
Size: 5381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 147 3331 3512 3675 147
Size: 3184 181 163 1853
Fragments arranged by size:
3184 1853 181 163
BtsI GCAGTG_nn'
Cuts at: 2624 2624
Size: 5381
DraI TTT'AAA
Cuts at: 605 2380 605
Size: 1775 3606
DrdI GACnn_nn'nnGTC
Cuts at: 3087 3945 3087
Size: 858 4523
EaeI y'GGCC_r
Cuts at: 187 419 2966 3140 3531 3558 4903 187
Size: 232 2547 174 391 27 1345 665
Fragments arranged by size:
2547 1345 665 391 232 174 27
EagI C'GGCC_G
Cuts at: 2966 2966
Size: 5381
EarI CTCTTCn'nnn_
Cuts at: 892 1483 3404 3614 892
Size: 591 1921 210 2659
Fragments arranged by size:
2659 1921 591 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3589 3899 4045 5191 3589
Size: 310 146 1146 3779
Fragments arranged by size:
3779 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2393 2393
Size: 5381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 1096 1110 1442 1457 1618 1972 3206 3638
Size: 801 14 332 15 161 354 1234 432
Cuts at: 3638 4385 4653 295
Size: 747 268 1023
Fragments arranged by size:
1234 1023 801 747 432 354 332 268
161 15 14
EcoNI CCTnn'n_nnAGG
Cuts at: 1060 1060
Size: 5381
EcoO109I rG'GnC_Cy
Cuts at: 548 567 1495 2520 548
Size: 19 928 1025 3409
Fragments arranged by size:
3409 1025 928 19
EcoRI G'AATT_C
Cuts at: 2385 2385
Size: 5381
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 894 1942 3400 894
Size: 1048 1458 2875
Fragments arranged by size:
2875 1458 1048
FspI TGC'GCA
Cuts at: 3162 3162
Size: 5381
HaeII r_GCGC'y
Cuts at: 2815 3063 4085 5064 2815
Size: 248 1022 979 3132
Fragments arranged by size:
3132 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 220 252 1969 2001 2054 2086 3223 3253 3255
Size: 32 1717 32 53 32 1137 30 2
Cuts at: 3255 3285 220
Size: 30 2316
Fragments arranged by size:
2316 1717 1137 53 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2192 2420 2192
Size: 228 5153
HindIII A'AGCT_T
Cuts at: 599 1282 1768 599
Size: 683 486 4212
Fragments arranged by size:
4212 683 486
KasI G'GCGC_C
Cuts at: 2811 3059 2811
Size: 248 5133
KpnI G_GTAC'C
Cuts at: 2401 2401
Size: 5381
MluI A'CGCG_T
Cuts at: 4814 4814
Size: 5381
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4052 4236 4052
Size: 184 5197
MscI TGG'CCA
Cuts at: 421 3142 421
Size: 2721 2660
MslI CAynn'nnrTG
Cuts at: 2095 3370 3497 2095
Size: 1275 127 3979
Fragments arranged by size:
3979 1275 127
MspA1I CmG'CkG
Cuts at: 186 466 2492 2806 3166 4179 4424 186
Size: 280 2026 314 360 1013 245 1143
Fragments arranged by size:
2026 1143 1013 360 314 280 245
NaeI GCC'GGC
Cuts at: 3562 3562
Size: 5381
NarI GG'CG_CC
Cuts at: 2812 3060 2812
Size: 248 5133
NgoMIV G'CCGG_C
Cuts at: 3560 3560
Size: 5381
NsiI A_TGCA'T
Cuts at: 1293 1293
Size: 5381
NspI r_CATG'y
Cuts at: 2434 2934 3841 2434
Size: 500 907 3974
Fragments arranged by size:
3974 907 500
PciI A'CATG_T
Cuts at: 3837 3837
Size: 5381
PflMI CCAn_nnn'nTGG
Cuts at: 535 903 1014 1254 1891 535
Size: 368 111 240 637 4025
Fragments arranged by size:
4025 637 368 240 111
PfoI T'CCnGG_A
Cuts at: 1073 1126 1634 1670 2239 1073
Size: 53 508 36 569 4215
Fragments arranged by size:
4215 569 508 53 36
PmeI GTTT'AAAC
Cuts at: 2380 2380
Size: 5381
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4520 4552 4520
Size: 32 5349
PpuMI rG'GwC_Cy
Cuts at: 548 567 1495 548
Size: 19 928 4434
Fragments arranged by size:
4434 928 19
PstI C_TGCA'G
Cuts at: 365 1301 1584 2333 2428 3113 365
Size: 936 283 749 95 685 2633
Fragments arranged by size:
2633 936 749 685 283 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5381
PvuII CAG'CTG
Cuts at: 466 2806 3166 466
Size: 2340 360 2681
Fragments arranged by size:
2681 2340 360
RsrII CG'GwC_CG
Cuts at: 3576 3576
Size: 5381
SacI G_AGCT'C
Cuts at: 2395 2395
Size: 5381
SalI G'TCGA_C
Cuts at: 2190 2418 2190
Size: 228 5153
SanDI GG'GwC_CC
Cuts at: 548 548
Size: 5381
SapI GCTCTTCn'nnn_
Cuts at: 3404 3614 3404
Size: 210 5171
SbfI CC_TGCA'GG
Cuts at: 2428 2428
Size: 5381
ScaI AGT'ACT
Cuts at: 4898 4898
Size: 5381
SfcI C'TryA_G
Cuts at: 33 361 1297 1580 2329 2424 3109 4102 4293
Size: 328 936 283 749 95 685 993 191
Cuts at: 4293 33
Size: 1121
Fragments arranged by size:
1121 993 936 749 685 328 283 191
95
SfoI GGC'GCC
Cuts at: 2813 3061 2813
Size: 248 5133
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5381
SmaI CCC'GGG
Cuts at: 1273 2403 1273
Size: 1130 4251
SmlI C'TyrA_G
Cuts at: 921 2536 3451 3943 4205 921
Size: 1615 915 492 262 2097
Fragments arranged by size:
2097 1615 915 492 262
SpeI A'CTAG_T
Cuts at: 67 352 1618 67
Size: 285 1266 3830
Fragments arranged by size:
3830 1266 285
SphI G_CATG'C
Cuts at: 2434 2934 2434
Size: 500 4881
SspI AAT'ATT
Cuts at: 753 753
Size: 5381
StuI AGG'CCT
Cuts at: 272 272
Size: 5381
StyI C'CwwG_G
Cuts at: 1724 2515 2869 1724
Size: 791 354 4236
Fragments arranged by size:
4236 791 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 989 1156 2964 3097 3556 3765 989
Size: 167 1808 133 459 209 2605
Fragments arranged by size:
2605 1808 459 209 167 133
TatI w'GTAC_w
Cuts at: 660 1133 2174 4896 660
Size: 473 1041 2722 1145
Fragments arranged by size:
2722 1145 1041 473
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 962 994 4898 4930 962
Size: 32 3904 32 1413
Fragments arranged by size:
3904 1413 32 32
Tth111I GACn'n_nGTC
Cuts at: 1209 3178 1209
Size: 1969 3412
XbaI T'CTAG_A
Cuts at: 2412 2412
Size: 5381
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1892 2876 114
Size: 1778 984 2619
Fragments arranged by size:
2619 1778 984
XmaI C'CCGG_G
Cuts at: 1271 2401 1271
Size: 1130 4251
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NsiI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SwaI XhoI XmnI ZraI