Restriction map for
pF1KB5769
(Circular) (Six-base) MAPSORT of: pF1KB5769.seq Check: 4528 from: 1 to: 5363
pF1KB5769 5363 bp
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 222 222
Size: 5363
AccI GT'mk_AC
Cuts at: 2401 2401
Size: 5363
Acc65I G'GTAC_C
Cuts at: 2165 2379 2165
Size: 214 5149
AclI AA'CG_TT
Cuts at: 5203 5203
Size: 5363
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 586 916 921 2029 3188 3620 4367 4635
Size: 320 330 5 1108 1159 432 747 268
Cuts at: 4635 266
Size: 994
Fragments arranged by size:
1159 1108 994 747 432 330 320 268
5
AflIII A'CryG_T
Cuts at: 2715 3819 4796 2715
Size: 1104 977 3282
Fragments arranged by size:
3282 1104 977
AgeI A'CCGG_T
Cuts at: 3751 3751
Size: 5363
AhdI GACnn_n'nnGTC
Cuts at: 618 618
Size: 5363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1330 1330
Size: 5363
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 856 888 856
Size: 32 5331
AlwNI CAG_nnn'CTG
Cuts at: 730 753 774 894 1056 2040 2668 4235 730
Size: 23 21 120 162 984 628 1567 1858
Fragments arranged by size:
1858 1567 984 628 162 120 23 21
ApaI G_GGCC'C
Cuts at: 160 1147 1619 160
Size: 987 472 3904
Fragments arranged by size:
3904 987 472
ApaLI G'TGCA_C
Cuts at: 4133 4133
Size: 5363
ApoI r'AATT_y
Cuts at: 807 2367 4716 4972 807
Size: 1560 2349 256 1198
Fragments arranged by size:
2349 1560 1198 256
AseI AT'TA_AT
Cuts at: 20 2125 20
Size: 2105 3258
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5363
AvaI C'yCGr_G
Cuts at: 173 2383 173
Size: 2210 3153
AvrII C'CTAG_G
Cuts at: 147 147
Size: 5363
BamHI G'GATC_C
Cuts at: 143 178 2388 143
Size: 35 2210 3118
Fragments arranged by size:
3118 2210 35
BanI G'GyrC_C
Cuts at: 1385 1624 1770 2043 2165 2379 2793 3041 3076
Size: 239 146 273 122 214 414 248 35
Cuts at: 3076 1385
Size: 3672
Fragments arranged by size:
3672 414 273 248 239 214 146 122
35
BanII G_rGCy'C
Cuts at: 160 483 556 671 1147 1513 1606 1619 2007
Size: 323 73 115 476 366 93 13 388
Cuts at: 2007 2377 3407 160
Size: 370 1030 2116
Fragments arranged by size:
2116 1030 476 388 370 366 323 115
93 73 13
BbeI G_GCGC'C
Cuts at: 2797 3045 2797
Size: 248 5115
BbsI GAAGACnn'nnnn_
Cuts at: 617 617
Size: 5363
BbvCI CC'TCA_GC
Cuts at: 991 1588 991
Size: 597 4766
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1018 1052 90
Size: 34 894 34 4401
Fragments arranged by size:
4401 894 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3261 4022 5363 3261
Size: 761 1341 3261
Fragments arranged by size:
3261 1341 761
BclI T'GATC_A
Cuts at: 2883 2883
Size: 5363
BglI GCCn_nnn'nGGC
Cuts at: 1030 1030
Size: 5363
BglII A'GATC_T
Cuts at: 940 2878 4915 940
Size: 1938 2037 1388
Fragments arranged by size:
2037 1938 1388
BlpI GC'TnA_GC
Cuts at: 1528 2475 1528
Size: 947 4416
Bme1580I G_kGCm'C
Cuts at: 160 195 1147 1619 1775 2988 3081 4137 160
Size: 35 952 472 156 1213 93 1056 1386
Fragments arranged by size:
1386 1213 1056 952 472 156 93 35
BmrI ACTGGGnnnn_n'
Cuts at: 224 1149 2575 2656 2991 5135 224
Size: 925 1426 81 335 2144 452
Fragments arranged by size:
2144 1426 925 452 335 81
BmtI G_CTAG'C
Cuts at: 491 491
Size: 5363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1463 3730 1463
Size: 2267 3096
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 1004 1513 2194 469
Size: 535 509 681 3638
Fragments arranged by size:
3638 681 535 509
Bpu10I CC'TnA_GC
Cuts at: 482 991 1129 1588 482
Size: 509 138 459 4257
Fragments arranged by size:
4257 509 459 138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2539 3418 3910 4208 2539
Size: 879 492 298 3694
Fragments arranged by size:
3694 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1668 1709 5067 1668
Size: 41 3358 1964
Fragments arranged by size:
3358 1964 41
BsaAI yAC'GTr
Cuts at: 2716 2716
Size: 5363
BsaBI GATnn'nnATC
Cuts at: 6 2210 2877 4914 6
Size: 2204 667 2037 455
Fragments arranged by size:
2204 2037 667 455
BsaHI Gr'CG_yC
Cuts at: 303 2794 3042 3744 5240 303
Size: 2491 248 702 1496 426
Fragments arranged by size:
2491 1496 702 426 248
BsaWI w'CCGG_w
Cuts at: 1999 2552 3073 3751 4025 4172 4583 1999
Size: 553 521 678 274 147 411 2779
Fragments arranged by size:
2779 678 553 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 442 472 856 886 1552 1582 1754 1784 2153
Size: 30 384 30 666 30 172 30 369
Cuts at: 2153 2183 442
Size: 30 3622
Fragments arranged by size:
3622 666 384 369 172 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 161 466 494 1203 1567 161
Size: 305 28 709 364 3957
Fragments arranged by size:
3957 709 364 305 28
BseYI C'CCAG_C
Cuts at: 153 182 342 556 650 1220 1774 2033 2286
Size: 29 160 214 94 570 554 259 253
Cuts at: 2286 2788 3546 4123 4619 153
Size: 502 758 577 496 897
Fragments arranged by size:
897 758 577 570 554 502 496 259
253 214 160 94 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 1300
Size: 5363
BsiEI CG_ry'CG
Cuts at: 104 1287 1454 1458 2905 2951 4159 104
Size: 1183 167 4 1447 46 1208 1308
Fragments arranged by size:
1447 1308 1208 1183 167 46 4
BsiHKAI G_wGCw'C
Cuts at: 1606 2377 3155 3345 4137 1606
Size: 771 778 190 792 2832
Fragments arranged by size:
2832 792 778 771 190
BsmBI CGTCTCn'nnnn_
Cuts at: 853 853
Size: 5363
Bsp1286I G_dGCh'C
Cuts at: 160 195 483 556 671 1147 1513 1606 1619
Size: 35 288 73 115 476 366 93 13
Cuts at: 1619 1775 2007 2377 2988 3081 3155 3345 3407
Size: 156 232 370 611 93 74 190 62
Cuts at: 3407 4137 160
Size: 730 1386
Fragments arranged by size:
1386 730 611 476 370 366 288 232
190 156 115 93 93 74 73 62
35 13
BspEI T'CCGG_A
Cuts at: 4583 4583
Size: 5363
BspHI T'CATG_A
Cuts at: 4539 4539
Size: 5363
BspMI ACCTGCnnnn'nnnn_
Cuts at: 222 1268 2413 2627 2929 3310 222
Size: 1046 1145 214 302 381 2275
Fragments arranged by size:
2275 1145 1046 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1413 1701 1847 3655 3709 5193 5358 50
Size: 1363 288 146 1808 54 1484 165 55
Fragments arranged by size:
1808 1484 1363 288 165 146 55 54
BsrDI GCAATG_nn'
Cuts at: 1793 3275 1793
Size: 1482 3881
BsrFI r'CCGG_y
Cuts at: 119 1238 1453 3361 3542 3751 119
Size: 1119 215 1908 181 209 1731
Fragments arranged by size:
1908 1731 1119 215 209 181
BssHII G'CGCG_C
Cuts at: 1391 1391
Size: 5363
BssSI C'ACGA_G
Cuts at: 1339 1363 3634 3992 1339
Size: 24 2271 358 2710
Fragments arranged by size:
2710 2271 358 24
BstAPI GCAn_nnn'nTGC
Cuts at: 2296 2740 2296
Size: 444 4919
BstBI TT'CG_AA
Cuts at: 270 3724 270
Size: 3454 1909
BstEII G'GTnAC_C
Cuts at: 1902 1995 1902
Size: 93 5270
BstXI CCAn_nnnn'nTGG
Cuts at: 137 1471 137
Size: 1334 4029
BstYI r'GATC_y
Cuts at: 1 143 178 595 940 2388 2855 2878 3213
Size: 142 35 417 345 1448 467 23 335
Cuts at: 3213 3459 4471 4557 4569 4915 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1448 1012 467 449 417 346 345 335
246 142 86 35 23 12
Bsu36I CC'TnA_GG
Cuts at: 923 1215 923
Size: 292 5071
BtgI C'CryG_G
Cuts at: 875 1520 875
Size: 645 4718
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1650 3313 3494 3657 1650
Size: 1663 181 163 3356
Fragments arranged by size:
3356 1663 181 163
BtsI GCAGTG_nn'
Cuts at: 1865 2050 2107 2606 1865
Size: 185 57 499 4622
Fragments arranged by size:
4622 499 185 57
DraI TTT'AAA
Cuts at: 2362 2362
Size: 5363
DrdI GACnn_nn'nnGTC
Cuts at: 1949 3069 3927 1949
Size: 1120 858 3385
Fragments arranged by size:
3385 1120 858
EaeI y'GGCC_r
Cuts at: 127 225 402 1284 1335 1455 2263 2948 3122
Size: 98 177 882 51 120 808 685 174
Cuts at: 3122 3513 3540 4885 127
Size: 391 27 1345 605
Fragments arranged by size:
1345 882 808 685 605 391 177 174
120 98 51 27
EagI C'GGCC_G
Cuts at: 1284 1455 2948 1284
Size: 171 1493 3699
Fragments arranged by size:
3699 1493 171
EarI CTCTTCn'nnn_
Cuts at: 892 1544 1666 2356 3386 3596 892
Size: 652 122 690 1030 210 2659
Fragments arranged by size:
2659 1030 690 652 210 122
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3571 3881 4027 5173 3571
Size: 310 146 1146 3761
Fragments arranged by size:
3761 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1604 2375 1604
Size: 771 4592
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 266 469 586 916 921 1004 1513 2029 2194
Size: 203 117 330 5 83 509 516 165
Cuts at: 2194 3188 3620 4367 4635 266
Size: 994 432 747 268 994
Fragments arranged by size:
994 994 747 516 509 432 330 268
203 165 117 83 5
EcoNI CCTnn'n_nnAGG
Cuts at: 604 604
Size: 5363
EcoO109I rG'GnC_Cy
Cuts at: 157 465 646 727 953 1032 1143 1615 2502
Size: 308 181 81 226 79 111 472 887
Cuts at: 2502 157
Size: 3018
Fragments arranged by size:
3018 887 472 308 226 181 111 81
79
EcoRI G'AATT_C
Cuts at: 2367 2367
Size: 5363
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1893 3382 1893
Size: 1489 3874
FspI TGC'GCA
Cuts at: 1645 3144 1645
Size: 1499 3864
HaeII r_GCGC'y
Cuts at: 1210 1535 2797 3045 4067 5046 1210
Size: 325 1262 248 1022 979 1527
Fragments arranged by size:
1527 1262 1022 979 325 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 170 202 442 474 604 636 769 801 1461
Size: 32 240 32 130 32 133 32 660
Cuts at: 1461 1493 1552 1584 3205 3235 3237 3267 170
Size: 32 59 32 1621 30 2 30 2266
Fragments arranged by size:
2266 1621 660 240 133 130 59 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 2402 1163
Size: 1239 4124
HindIII A'AGCT_T
Cuts at: 1870 2011 1870
Size: 141 5222
HpaI GTT'AAC
Cuts at: 1163 1163
Size: 5363
KasI G'GCGC_C
Cuts at: 2793 3041 2793
Size: 248 5115
KpnI G_GTAC'C
Cuts at: 2169 2383 2169
Size: 214 5149
MfeI C'AATT_G
Cuts at: 406 406
Size: 5363
MluI A'CGCG_T
Cuts at: 4796 4796
Size: 5363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 308 409 1337 1474 1538 1718 4034 4218 308
Size: 101 928 137 64 180 2316 184 1453
Fragments arranged by size:
2316 1453 928 184 180 137 101 64
MscI TGG'CCA
Cuts at: 129 227 1337 3124 129
Size: 98 1110 1787 2368
Fragments arranged by size:
2368 1787 1110 98
MslI CAynn'nnrTG
Cuts at: 1990 2069 3352 3479 1990
Size: 79 1283 127 3874
Fragments arranged by size:
3874 1283 127 79
MspA1I CmG'CkG
Cuts at: 704 1231 1522 1578 1755 2316 2325 2474 2788
Size: 527 291 56 177 561 9 149 314
Cuts at: 2788 3148 4161 4406 704
Size: 360 1013 245 1661
Fragments arranged by size:
1661 1013 561 527 360 314 291 245
177 149 56 9
NaeI GCC'GGC
Cuts at: 1240 3544 1240
Size: 2304 3059
NarI GG'CG_CC
Cuts at: 2794 3042 2794
Size: 248 5115
NcoI C'CATG_G
Cuts at: 875 875
Size: 5363
NgoMIV G'CCGG_C
Cuts at: 1238 3542 1238
Size: 2304 3059
NheI G'CTAG_C
Cuts at: 487 487
Size: 5363
NruI TCG'CGA
Cuts at: 1669 1669
Size: 5363
NspI r_CATG'y
Cuts at: 737 2416 2916 3823 737
Size: 1679 500 907 2277
Fragments arranged by size:
2277 1679 907 500
PasI CC'CwG_GG
Cuts at: 2308 2308
Size: 5363
PciI A'CATG_T
Cuts at: 3819 3819
Size: 5363
PflMI CCAn_nnn'nTGG
Cuts at: 122 1074 2040 2232 122
Size: 952 966 192 3253
Fragments arranged by size:
3253 966 952 192
PfoI T'CCnGG_A
Cuts at: 575 829 1055 2021 2171 575
Size: 254 226 966 150 3767
Fragments arranged by size:
3767 966 254 226 150
PmeI GTTT'AAAC
Cuts at: 2362 2362
Size: 5363
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 856 888 4502 4534 856
Size: 32 3614 32 1685
Fragments arranged by size:
3614 1685 32 32
PpuMI rG'GwC_Cy
Cuts at: 953 953
Size: 5363
PspOMI G'GGCC_C
Cuts at: 156 1143 1615 156
Size: 987 472 3904
Fragments arranged by size:
3904 987 472
PstI C_TGCA'G
Cuts at: 749 1355 1697 2410 3095 749
Size: 606 342 713 685 3017
Fragments arranged by size:
3017 713 685 606 342
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5363
PvuII CAG'CTG
Cuts at: 2316 2325 2788 3148 2316
Size: 9 463 360 4531
Fragments arranged by size:
4531 463 360 9
RsrII CG'GwC_CG
Cuts at: 3558 3558
Size: 5363
SacI G_AGCT'C
Cuts at: 1606 2377 1606
Size: 771 4592
SacII CC_GC'GG
Cuts at: 1523 1523
Size: 5363
SalI G'TCGA_C
Cuts at: 2400 2400
Size: 5363
SanDI GG'GwC_CC
Cuts at: 953 953
Size: 5363
SapI GCTCTTCn'nnn_
Cuts at: 3386 3596 3386
Size: 210 5153
SbfI CC_TGCA'GG
Cuts at: 1697 2410 1697
Size: 713 4650
ScaI AGT'ACT
Cuts at: 1971 4880 1971
Size: 2909 2454
SexAI A'CCwGG_T
Cuts at: 686 1718 686
Size: 1032 4331
SfcI C'TryA_G
Cuts at: 33 745 1351 1693 2406 3091 4084 4275 33
Size: 712 606 342 713 685 993 191 1121
Fragments arranged by size:
1121 993 713 712 685 606 342 191
SfoI GGC'GCC
Cuts at: 2795 3043 2795
Size: 248 5115
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5363
SmaI CCC'GGG
Cuts at: 2385 2385
Size: 5363
SmlI C'TyrA_G
Cuts at: 2518 3433 3925 4187 2518
Size: 915 492 262 3694
Fragments arranged by size:
3694 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5363
SphI G_CATG'C
Cuts at: 737 2416 2916 737
Size: 1679 500 3184
Fragments arranged by size:
3184 1679 500
StyI C'CwwG_G
Cuts at: 147 875 1113 1464 2497 2851 147
Size: 728 238 351 1033 354 2659
Fragments arranged by size:
2659 1033 728 354 351 238
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 701 1143 1416 2946 3079 3538 3747 701
Size: 442 273 1530 133 459 209 2317
Fragments arranged by size:
2317 1530 459 442 273 209 133
TatI w'GTAC_w
Cuts at: 677 1969 4878 677
Size: 1292 2909 1162
Fragments arranged by size:
2909 1292 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1754 1786 4880 4912 1754
Size: 32 3094 32 2205
Fragments arranged by size:
3094 2205 32 32
Tth111I GACn'n_nGTC
Cuts at: 3160 3160
Size: 5363
XbaI T'CTAG_A
Cuts at: 1818 2394 1818
Size: 576 4787
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2858 2858
Size: 5363
XmaI C'CCGG_G
Cuts at: 2383 2383
Size: 5363
XmnI GAAnn'nnTTC
Cuts at: 571 571
Size: 5363
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NruI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI BaeI BfrBI BmgBI
BsiWI BsmI BsrGI BstZ17I ClaI CspCI DraIII EcoRV
FseI FspAI NdeI NotI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI