Restriction map for
pF1KB5787
(Circular) (Six-base) MAPSORT of: pF1KB5787.seq Check: 9913 from: 1 to: 5600
pF1KB5787 5600 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 804 804
Size: 5600
AccI GT'mk_AC
Cuts at: 1490 2638 1490
Size: 1148 4452
Acc65I G'GTAC_C
Cuts at: 2616 2616
Size: 5600
AclI AA'CG_TT
Cuts at: 5440 5440
Size: 5600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 183 230 388 1381 1945 1957 2413 3425 3857
Size: 47 158 993 564 12 456 1012 432
Cuts at: 3857 4604 4872 183
Size: 747 268 911
Fragments arranged by size:
1012 993 911 747 564 456 432 268
158 47 12
AflIII A'CryG_T
Cuts at: 2570 2952 4056 5033 2570
Size: 382 1104 977 3137
Fragments arranged by size:
3137 1104 977 382
AgeI A'CCGG_T
Cuts at: 3988 3988
Size: 5600
AhdI GACnn_n'nnGTC
Cuts at: 2475 2475
Size: 5600
AleI CACnn'nnGTG
Cuts at: 1042 1188 1387 1597 1042
Size: 146 199 210 5045
Fragments arranged by size:
5045 210 199 146
AlwNI CAG_nnn'CTG
Cuts at: 258 489 1152 1750 1800 2905 4472 258
Size: 231 663 598 50 1105 1567 1386
Fragments arranged by size:
1567 1386 1105 663 598 231 50
ApaLI G'TGCA_C
Cuts at: 1957 4370 1957
Size: 2413 3187
ApoI r'AATT_y
Cuts at: 2604 4953 5209 2604
Size: 2349 256 2995
Fragments arranged by size:
2995 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5600
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5600
AvaI C'yCGr_G
Cuts at: 2620 2620
Size: 5600
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 791 824 2320 2353 791
Size: 33 1496 33 4038
Fragments arranged by size:
4038 1496 33 33
BamHI G'GATC_C
Cuts at: 2625 2625
Size: 5600
BanI G'GyrC_C
Cuts at: 682 937 1416 1603 2310 2616 3030 3278 3313
Size: 255 479 187 707 306 414 248 35
Cuts at: 3313 682
Size: 2969
Fragments arranged by size:
2969 707 479 414 306 255 248 187
35
BanII G_rGCy'C
Cuts at: 116 248 656 1757 2165 2488 2614 3644 116
Size: 132 408 1101 408 323 126 1030 2072
Fragments arranged by size:
2072 1101 1030 408 408 323 132 126
BbeI G_GCGC'C
Cuts at: 3034 3282 3034
Size: 248 5352
BbsI GAAGACnn'nnnn_
Cuts at: 189 423 570 1508 2193 189
Size: 234 147 938 685 3596
Fragments arranged by size:
3596 938 685 234 147
BciVI GTATCCnnnnn_n'
Cuts at: 1938 3498 4259 5600 1938
Size: 1560 761 1341 1938
Fragments arranged by size:
1938 1560 1341 761
BclI T'GATC_A
Cuts at: 2003 3120 2003
Size: 1117 4483
BglII A'GATC_T
Cuts at: 389 3115 5152 389
Size: 2726 2037 837
Fragments arranged by size:
2726 2037 837
BlpI GC'TnA_GC
Cuts at: 2712 2712
Size: 5600
Bme1580I G_kGCm'C
Cuts at: 1606 1961 3225 3318 4374 1606
Size: 355 1264 93 1056 2832
Fragments arranged by size:
2832 1264 1056 355 93
BmgBI CAC'GTC
Cuts at: 1956 2307 1956
Size: 351 5249
BmrI ACTGGGnnnn_n'
Cuts at: 1481 1738 2812 2893 3228 5372 1481
Size: 257 1074 81 335 2144 1709
Fragments arranged by size:
2144 1709 1074 335 257 81
BmtI G_CTAG'C
Cuts at: 1567 1567
Size: 5600
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3967 3967
Size: 5600
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 939 2293 133
Size: 806 1354 3440
Fragments arranged by size:
3440 1354 806
Bpu10I CC'TnA_GC
Cuts at: 1751 2482 1751
Size: 731 4869
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 2776 3655 4147 4445 484
Size: 2292 879 492 298 1639
Fragments arranged by size:
2292 1639 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1541 5304 1541
Size: 3763 1837
BsaAI yAC'GTr
Cuts at: 1398 2953 1398
Size: 1555 4045
BsaBI GATnn'nnATC
Cuts at: 6 1768 3114 5151 6
Size: 1762 1346 2037 455
Fragments arranged by size:
2037 1762 1346 455
BsaHI Gr'CG_yC
Cuts at: 1172 2471 3031 3279 3981 5477 1172
Size: 1299 560 248 702 1496 1295
Fragments arranged by size:
1496 1299 1295 702 560 248
BsaWI w'CCGG_w
Cuts at: 926 2789 3310 3988 4262 4409 4820 926
Size: 1863 521 678 274 147 411 1706
Fragments arranged by size:
1863 1706 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1468 1498 1468
Size: 30 5570
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 130 649 2487 130
Size: 519 1838 3243
Fragments arranged by size:
3243 1838 519
BseYI C'CCAG_C
Cuts at: 258 1551 1850 2085 2255 3025 3783 4360 4856
Size: 1293 299 235 170 770 758 577 496
Cuts at: 4856 258
Size: 1002
Fragments arranged by size:
1293 1002 770 758 577 496 299 235
170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1165 1165
Size: 5600
BsiEI CG_ry'CG
Cuts at: 104 1614 3142 3188 4396 104
Size: 1510 1528 46 1208 1308
Fragments arranged by size:
1528 1510 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 656 934 1961 2488 2614 3392 3582 4374 656
Size: 278 1027 527 126 778 190 792 1882
Fragments arranged by size:
1882 1027 792 778 527 278 190 126
BsmI GAATG_Cn'
Cuts at: 1131 1131
Size: 5600
BsmBI CGTCTCn'nnnn_
Cuts at: 140 140
Size: 5600
Bsp1286I G_dGCh'C
Cuts at: 116 248 656 934 1606 1757 1961 2165 2488
Size: 132 408 278 672 151 204 204 323
Cuts at: 2488 2614 3225 3318 3392 3582 3644 4374 116
Size: 126 611 93 74 190 62 730 1342
Fragments arranged by size:
1342 730 672 611 408 323 278 204
204 190 151 132 126 93 74 62
BspEI T'CCGG_A
Cuts at: 4820 4820
Size: 5600
BspHI T'CATG_A
Cuts at: 4776 4776
Size: 5600
BspMI ACCTGCnnnn'nnnn_
Cuts at: 804 1578 2053 2650 2864 3166 3547 804
Size: 774 475 597 214 302 381 2857
Fragments arranged by size:
2857 774 597 475 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3892 3946 5430 5595 50
Size: 3842 54 1484 165 55
Fragments arranged by size:
3842 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2307 3512 2307
Size: 1205 4395
BsrFI r'CCGG_y
Cuts at: 3598 3779 3988 3598
Size: 181 209 5210
Fragments arranged by size:
5210 209 181
BssSI C'ACGA_G
Cuts at: 119 3871 4229 119
Size: 3752 358 1490
Fragments arranged by size:
3752 1490 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2977 2977
Size: 5600
BstBI TT'CG_AA
Cuts at: 3961 3961
Size: 5600
BstXI CCAn_nnnn'nTGG
Cuts at: 963 1014 1044 1389 1559 963
Size: 51 30 345 170 5004
Fragments arranged by size:
5004 345 170 51 30
BstYI r'GATC_y
Cuts at: 1 389 1058 2625 3092 3115 3450 3696 4708
Size: 388 669 1567 467 23 335 246 1012
Cuts at: 4708 4794 4806 5152 1
Size: 86 12 346 449
Fragments arranged by size:
1567 1012 669 467 449 388 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 569 569
Size: 5600
BtgI C'CryG_G
Cuts at: 107 824 1037 1568 1667 1737 1977 2028 107
Size: 717 213 531 99 70 240 51 3679
Fragments arranged by size:
3679 717 531 240 213 99 70 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3550 3731 3894 3550
Size: 181 163 5256
Fragments arranged by size:
5256 181 163
BtsI GCAGTG_nn'
Cuts at: 172 1049 1136 1493 2843 172
Size: 877 87 357 1350 2929
Fragments arranged by size:
2929 1350 877 357 87
DraI TTT'AAA
Cuts at: 2599 2599
Size: 5600
DraIII CAC_nnn'GTG
Cuts at: 669 2018 669
Size: 1349 4251
DrdI GACnn_nn'nnGTC
Cuts at: 1912 3306 4164 1912
Size: 1394 858 3348
Fragments arranged by size:
3348 1394 858
EaeI y'GGCC_r
Cuts at: 249 660 1664 2031 2347 3185 3359 3750 3777
Size: 411 1004 367 316 838 174 391 27
Cuts at: 3777 5122 249
Size: 1345 727
Fragments arranged by size:
1345 1004 838 727 411 391 367 316
174 27
EagI C'GGCC_G
Cuts at: 3185 3185
Size: 5600
EarI CTCTTCn'nnn_
Cuts at: 2210 2225 3623 3833 2210
Size: 15 1398 210 3977
Fragments arranged by size:
3977 1398 210 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2490 3808 4118 4264 5410 2490
Size: 1318 310 146 1146 2680
Fragments arranged by size:
2680 1318 1146 310 146
EcoICRI GAG'CTC
Cuts at: 654 2486 2612 654
Size: 1832 126 3642
Fragments arranged by size:
3642 1832 126
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 183 230 388 939 1381 1945 1957 2293
Size: 50 47 158 551 442 564 12 336
Cuts at: 2293 2413 3425 3857 4604 4872 133
Size: 120 1012 432 747 268 861
Fragments arranged by size:
1012 861 747 564 551 442 432 336
268 158 120 50 47 12
EcoNI CCTnn'n_nnAGG
Cuts at: 148 1939 148
Size: 1791 3809
EcoO109I rG'GnC_Cy
Cuts at: 472 696 897 1347 1818 2739 472
Size: 224 201 450 471 921 3333
Fragments arranged by size:
3333 921 471 450 224 201
EcoRI G'AATT_C
Cuts at: 2604 2604
Size: 5600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 492 3619 492
Size: 3127 2473
FspI TGC'GCA
Cuts at: 3381 3381
Size: 5600
HaeII r_GCGC'y
Cuts at: 259 3034 3282 4304 5283 259
Size: 2775 248 1022 979 576
Fragments arranged by size:
2775 1022 979 576 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 477 509 761 793 2057 2089 2461 2493 3442
Size: 32 252 32 1264 32 372 32 949
Cuts at: 3442 3472 3474 3504 477
Size: 30 2 30 2573
Fragments arranged by size:
2573 1264 949 372 252 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1893 2639 1893
Size: 746 4854
KasI G'GCGC_C
Cuts at: 3030 3278 3030
Size: 248 5352
KpnI G_GTAC'C
Cuts at: 2620 2620
Size: 5600
MluI A'CGCG_T
Cuts at: 5033 5033
Size: 5600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2531 4271 4455 2492
Size: 39 1740 184 3637
Fragments arranged by size:
3637 1740 184 39
MscI TGG'CCA
Cuts at: 662 1666 2033 3361 662
Size: 1004 367 1328 2901
Fragments arranged by size:
2901 1328 1004 367
MslI CAynn'nnrTG
Cuts at: 718 1012 1042 1188 1387 1597 2033 3589 3716
Size: 294 30 146 199 210 436 1556 127
Cuts at: 3716 718
Size: 2602
Fragments arranged by size:
2602 1556 436 294 210 199 146 127
30
MspA1I CmG'CkG
Cuts at: 1597 1995 2462 2711 3025 3385 4398 4643 1597
Size: 398 467 249 314 360 1013 245 2554
Fragments arranged by size:
2554 1013 467 398 360 314 249 245
NaeI GCC'GGC
Cuts at: 3781 3781
Size: 5600
NarI GG'CG_CC
Cuts at: 3031 3279 3031
Size: 248 5352
NcoI C'CATG_G
Cuts at: 107 1568 1667 1737 1977 2028 107
Size: 1461 99 70 240 51 3679
Fragments arranged by size:
3679 1461 240 99 70 51
NdeI CA'TA_TG
Cuts at: 1950 1950
Size: 5600
NgoMIV G'CCGG_C
Cuts at: 3779 3779
Size: 5600
NheI G'CTAG_C
Cuts at: 1563 1563
Size: 5600
NspI r_CATG'y
Cuts at: 2574 2653 3153 4060 2574
Size: 79 500 907 4114
Fragments arranged by size:
4114 907 500 79
PasI CC'CwG_GG
Cuts at: 283 283
Size: 5600
PciI A'CATG_T
Cuts at: 2570 4056 2570
Size: 1486 4114
PflMI CCAn_nnn'nTGG
Cuts at: 258 1582 258
Size: 1324 4276
PfoI T'CCnGG_A
Cuts at: 350 1454 350
Size: 1104 4496
PmeI GTTT'AAAC
Cuts at: 2599 2599
Size: 5600
PmlI CAC'GTG
Cuts at: 1398 1398
Size: 5600
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 4739
Size: 32 5568
PpuMI rG'GwC_Cy
Cuts at: 472 696 897 1818 472
Size: 224 201 921 4254
Fragments arranged by size:
4254 921 224 201
PsiI TTA'TAA
Cuts at: 2380 2380
Size: 5600
PstI C_TGCA'G
Cuts at: 167 1500 2647 3332 167
Size: 1333 1147 685 2435
Fragments arranged by size:
2435 1333 1147 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5600
PvuII CAG'CTG
Cuts at: 1597 1995 3025 3385 1597
Size: 398 1030 360 3812
Fragments arranged by size:
3812 1030 398 360
RsrII CG'GwC_CG
Cuts at: 3795 3795
Size: 5600
SacI G_AGCT'C
Cuts at: 656 2488 2614 656
Size: 1832 126 3642
Fragments arranged by size:
3642 1832 126
SalI G'TCGA_C
Cuts at: 2637 2637
Size: 5600
SanDI GG'GwC_CC
Cuts at: 472 472
Size: 5600
SapI GCTCTTCn'nnn_
Cuts at: 3623 3833 3623
Size: 210 5390
SbfI CC_TGCA'GG
Cuts at: 2647 2647
Size: 5600
ScaI AGT'ACT
Cuts at: 5117 5117
Size: 5600
SexAI A'CCwGG_T
Cuts at: 795 795
Size: 5600
SfcI C'TryA_G
Cuts at: 33 163 534 584 1496 2643 3328 4321 4512
Size: 130 371 50 912 1147 685 993 191
Cuts at: 4512 33
Size: 1121
Fragments arranged by size:
1147 1121 993 912 685 371 191 130
50
SfoI GGC'GCC
Cuts at: 3032 3280 3032
Size: 248 5352
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5600
SmaI CCC'GGG
Cuts at: 2622 2622
Size: 5600
SmlI C'TyrA_G
Cuts at: 499 2755 3670 4162 4424 499
Size: 2256 915 492 262 1675
Fragments arranged by size:
2256 1675 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5600
SphI G_CATG'C
Cuts at: 2653 3153 2653
Size: 500 5100
StyI C'CwwG_G
Cuts at: 107 218 1568 1667 1737 1977 2028 2734 3088
Size: 111 1350 99 70 240 51 706 354
Cuts at: 3088 107
Size: 2619
Fragments arranged by size:
2619 1350 706 354 240 111 99 70
51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 423 739 1264 2269 2346 3183 3316 3775 3984
Size: 316 525 1005 77 837 133 459 209
Cuts at: 3984 423
Size: 2039
Fragments arranged by size:
2039 1005 837 525 459 316 209 133
77
TatI w'GTAC_w
Cuts at: 986 5115 986
Size: 4129 1471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 983 1015 1468 1500 5117 5149 983
Size: 32 453 32 3617 32 1434
Fragments arranged by size:
3617 1434 453 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2197 3397 2197
Size: 1200 4400
XbaI T'CTAG_A
Cuts at: 2631 2631
Size: 5600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1041 1290 2286 3095 1041
Size: 249 996 809 3546
Fragments arranged by size:
3546 996 809 249
XmaI C'CCGG_G
Cuts at: 2620 2620
Size: 5600
XmnI GAAnn'nnTTC
Cuts at: 268 391 1930 268
Size: 123 1539 3938
Fragments arranged by size:
3938 1539 123
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI AloI ApaI AscI AvrII
BbvCI BcgI BfrBI BglI BsiWI BsrGI BssHII BstEII
BstZ17I ClaI CspCI EcoRV FseI FspAI HindIII HpaI
MfeI NotI NruI NsiI PacI PshAI PspOMI PspXI
PsrI SacII SfiI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI