Restriction map for
pF1KB5789
(Circular) (Six-base) MAPSORT of: pF1KB5789.seq Check: 7726 from: 1 to: 5600
pF1KB5789 5600 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 2638 2638
Size: 5600
Acc65I G'GTAC_C
Cuts at: 520 2616 520
Size: 2096 3504
AclI AA'CG_TT
Cuts at: 5440 5440
Size: 5600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 542 1187 1280 1377 2602 3425 3857 4604
Size: 261 645 93 97 1225 823 432 747
Cuts at: 4604 4872 281
Size: 268 1009
Fragments arranged by size:
1225 1009 823 747 645 432 268 261
97 93
AfeI AGC'GCT
Cuts at: 2049 2049
Size: 5600
AflIII A'CryG_T
Cuts at: 417 641 1402 2952 4056 5033 417
Size: 224 761 1550 1104 977 984
Fragments arranged by size:
1550 1104 984 977 761 224
AgeI A'CCGG_T
Cuts at: 3988 3988
Size: 5600
AleI CACnn'nnGTG
Cuts at: 820 896 820
Size: 76 5524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2315 2347 2315
Size: 32 5568
AlwNI CAG_nnn'CTG
Cuts at: 331 1165 1239 1627 1649 1741 1974 2373 2905
Size: 834 74 388 22 92 233 399 532
Cuts at: 2905 4472 331
Size: 1567 1459
Fragments arranged by size:
1567 1459 834 532 399 388 233 92
74 22
ApaI G_GGCC'C
Cuts at: 310 357 310
Size: 47 5553
ApaLI G'TGCA_C
Cuts at: 448 910 1957 4370 448
Size: 462 1047 2413 1678
Fragments arranged by size:
2413 1678 1047 462
ApoI r'AATT_y
Cuts at: 213 1035 1942 2604 4953 5209 213
Size: 822 907 662 2349 256 604
Fragments arranged by size:
2349 907 822 662 604 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5600
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5600
AvaI C'yCGr_G
Cuts at: 936 1501 1543 2620 936
Size: 565 42 1077 3916
Fragments arranged by size:
3916 1077 565 42
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1154 1187 1154
Size: 33 5567
BamHI G'GATC_C
Cuts at: 321 2625 321
Size: 2304 3296
BanI G'GyrC_C
Cuts at: 474 520 1981 2255 2482 2616 3030 3278 3313
Size: 46 1461 274 227 134 414 248 35
Cuts at: 3313 474
Size: 2761
Fragments arranged by size:
2761 1461 414 274 248 227 134 46
35
BanII G_rGCy'C
Cuts at: 310 357 507 672 1032 1151 1246 1502 1550
Size: 47 150 165 360 119 95 256 48
Cuts at: 1550 2614 3644 310
Size: 1064 1030 2266
Fragments arranged by size:
2266 1064 1030 360 256 165 150 119
95 48 47
BbeI G_GCGC'C
Cuts at: 1985 2259 3034 3282 1985
Size: 274 775 248 4303
Fragments arranged by size:
4303 775 274 248
BbvCI CC'TCA_GC
Cuts at: 679 679
Size: 5600
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1036 1070 1036
Size: 34 5566
BciVI GTATCCnnnnn_n'
Cuts at: 2019 3498 4259 5600 2019
Size: 1479 761 1341 2019
Fragments arranged by size:
2019 1479 1341 761
BclI T'GATC_A
Cuts at: 1437 3120 1437
Size: 1683 3917
BglII A'GATC_T
Cuts at: 3115 5152 3115
Size: 2037 3563
BlpI GC'TnA_GC
Cuts at: 511 2712 511
Size: 2201 3399
Bme1580I G_kGCm'C
Cuts at: 310 357 452 479 914 1839 1961 2120 2204
Size: 47 95 27 435 925 122 159 84
Cuts at: 2204 2579 3225 3318 4374 310
Size: 375 646 93 1056 1536
Fragments arranged by size:
1536 1056 925 646 435 375 159 122
95 93 84 47 27
BmrI ACTGGGnnnn_n'
Cuts at: 1962 2812 2893 3228 5372 1962
Size: 850 81 335 2144 2190
Fragments arranged by size:
2190 2144 850 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 635 1641 1962 3967 635
Size: 1006 321 2005 2268
Fragments arranged by size:
2268 2005 1006 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 605 673 736 915 943 1003 1087 1763
Size: 184 68 63 179 28 60 84 676
Cuts at: 1763 2245 421
Size: 482 3776
Fragments arranged by size:
3776 676 482 184 179 84 68 63
60 28
Bpu10I CC'TnA_GC
Cuts at: 610 679 1145 1240 1247 610
Size: 69 466 95 7 4963
Fragments arranged by size:
4963 466 95 69 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 473 571 2776 3655 4147 4445 473
Size: 98 2205 879 492 298 1628
Fragments arranged by size:
2205 1628 879 492 298 98
BsaI GGTCTCn'nnnn_
Cuts at: 5304 5304
Size: 5600
BsaAI yAC'GTr
Cuts at: 453 975 1077 1403 2320 2953 453
Size: 522 102 326 917 633 3100
Fragments arranged by size:
3100 917 633 522 326 102
BsaBI GATnn'nnATC
Cuts at: 6 3114 5151 6
Size: 3108 2037 455
Fragments arranged by size:
3108 2037 455
BsaHI Gr'CG_yC
Cuts at: 1982 2256 3031 3279 3981 5477 1982
Size: 274 775 248 702 1496 2105
Fragments arranged by size:
2105 1496 775 702 274 248
BsaWI w'CCGG_w
Cuts at: 2789 3310 3988 4262 4409 4820 2789
Size: 521 678 274 147 411 3569
Fragments arranged by size:
3569 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 888 918 2398 2428 888
Size: 30 1480 30 4060
Fragments arranged by size:
4060 1480 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 382
Size: 5600
BseYI C'CCAG_C
Cuts at: 167 626 663 1311 1839 1985 2094 2173 3025
Size: 459 37 648 528 146 109 79 852
Cuts at: 3025 3783 4360 4856 167
Size: 758 577 496 911
Fragments arranged by size:
911 852 758 648 577 528 496 459
146 109 79 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1057 1723 2039 2118 1057
Size: 666 316 79 4539
Fragments arranged by size:
4539 666 316 79
BsiEI CG_ry'CG
Cuts at: 104 3142 3188 4396 104
Size: 3038 46 1208 1308
Fragments arranged by size:
3038 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 452 507 697 817 914 1151 1322 1961 2614
Size: 55 190 120 97 237 171 639 653
Cuts at: 2614 3392 3582 4374 452
Size: 778 190 792 1678
Fragments arranged by size:
1678 792 778 653 639 237 190 190
171 120 97 55
BsmI GAATG_Cn'
Cuts at: 960 960
Size: 5600
Bsp1286I G_dGCh'C
Cuts at: 310 357 452 479 507 672 697 817 914
Size: 47 95 27 28 165 25 120 97
Cuts at: 914 1032 1151 1246 1322 1502 1550 1839 1961
Size: 118 119 95 76 180 48 289 122
Cuts at: 1961 2120 2204 2579 2614 3225 3318 3392 3582
Size: 159 84 375 35 611 93 74 190
Cuts at: 3582 3644 4374 310
Size: 62 730 1536
Fragments arranged by size:
1536 730 611 375 289 190 180 165
159 122 120 119 118 97 95 95
93 84 76 74 62 48 47 35
28 27 25
BspEI T'CCGG_A
Cuts at: 4820 4820
Size: 5600
BspHI T'CATG_A
Cuts at: 1683 4776 1683
Size: 3093 2507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1022 2650 2864 3166 3547 1022
Size: 1628 214 302 381 3075
Fragments arranged by size:
3075 1628 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3892 3946 5430 5595 50
Size: 3842 54 1484 165 55
Fragments arranged by size:
3842 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3512 3512
Size: 5600
BsrFI r'CCGG_y
Cuts at: 1843 3598 3779 3988 1843
Size: 1755 181 209 3455
Fragments arranged by size:
3455 1755 209 181
BssSI C'ACGA_G
Cuts at: 3871 4229 3871
Size: 358 5242
BstAPI GCAn_nnn'nTGC
Cuts at: 1192 2418 2977 1192
Size: 1226 559 3815
Fragments arranged by size:
3815 1226 559
BstBI TT'CG_AA
Cuts at: 3961 3961
Size: 5600
BstEII G'GTnAC_C
Cuts at: 345 393 757 345
Size: 48 364 5188
Fragments arranged by size:
5188 364 48
BstXI CCAn_nnnn'nTGG
Cuts at: 357 987 1371 2152 357
Size: 630 384 781 3805
Fragments arranged by size:
3805 781 630 384
BstYI r'GATC_y
Cuts at: 1 321 591 2625 3092 3115 3450 3696 4708
Size: 320 270 2034 467 23 335 246 1012
Cuts at: 4708 4794 4806 5152 1
Size: 86 12 346 449
Fragments arranged by size:
2034 1012 467 449 346 335 320 270
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 768 1253 1487 1894 768
Size: 485 234 407 4474
Fragments arranged by size:
4474 485 407 234
BtgI C'CryG_G
Cuts at: 107 791 1041 1364 1754 2151 2237 2336 107
Size: 684 250 323 390 397 86 99 3371
Fragments arranged by size:
3371 684 397 390 323 250 99 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3550 3731 3894 3550
Size: 181 163 5256
Fragments arranged by size:
5256 181 163
BtsI GCAGTG_nn'
Cuts at: 782 1308 2419 2471 2843 782
Size: 526 1111 52 372 3539
Fragments arranged by size:
3539 1111 526 372 52
DraI TTT'AAA
Cuts at: 2599 2599
Size: 5600
DraIII CAC_nnn'GTG
Cuts at: 1227 1227
Size: 5600
DrdI GACnn_nn'nnGTC
Cuts at: 1132 1179 3306 4164 1132
Size: 47 2127 858 2568
Fragments arranged by size:
2568 2127 858 47
EaeI y'GGCC_r
Cuts at: 1361 1514 2192 3185 3359 3750 3777 5122 1361
Size: 153 678 993 174 391 27 1345 1839
Fragments arranged by size:
1839 1345 993 678 391 174 153 27
EagI C'GGCC_G
Cuts at: 3185 3185
Size: 5600
EarI CTCTTCn'nnn_
Cuts at: 550 3623 3833 550
Size: 3073 210 2317
Fragments arranged by size:
3073 2317 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1546 3808 4118 4264 5410 1546
Size: 2262 310 146 1146 1736
Fragments arranged by size:
2262 1736 1146 310 146
EcoICRI GAG'CTC
Cuts at: 505 1149 2612 505
Size: 644 1463 3493
Fragments arranged by size:
3493 1463 644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 281 421 542 605 673 736 915 943 1003
Size: 140 121 63 68 63 179 28 60
Cuts at: 1003 1087 1187 1280 1377 1763 2245 2602 3425
Size: 84 100 93 97 386 482 357 823
Cuts at: 3425 3857 4604 4872 281
Size: 432 747 268 1009
Fragments arranged by size:
1009 823 747 482 432 386 357 268
179 140 121 100 97 93 84 68
63 63 60 28
EcoNI CCTnn'n_nnAGG
Cuts at: 613 1018 1486 613
Size: 405 468 4727
Fragments arranged by size:
4727 468 405
EcoO109I rG'GnC_Cy
Cuts at: 306 353 354 722 940 1738 2082 2181 2550
Size: 47 1 368 218 798 344 99 369
Cuts at: 2550 2739 306
Size: 189 3167
Fragments arranged by size:
3167 798 369 368 344 218 189 99
47 1
EcoRI G'AATT_C
Cuts at: 1035 2604 1035
Size: 1569 4031
EcoRV GAT'ATC
Cuts at: 1431 1431
Size: 5600
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3619 3619
Size: 5600
FspI TGC'GCA
Cuts at: 2422 3381 2422
Size: 959 4641
HaeII r_GCGC'y
Cuts at: 1985 2051 2259 3034 3282 4304 5283 1985
Size: 66 208 775 248 1022 979 2302
Fragments arranged by size:
2302 1022 979 775 248 208 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 590 622 751 783 1631 1663 2376 2408 3442
Size: 32 129 32 848 32 713 32 1034
Cuts at: 3442 3472 3474 3504 590
Size: 30 2 30 2686
Fragments arranged by size:
2686 1034 848 713 129 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 2639 2639
Size: 5600
KasI G'GCGC_C
Cuts at: 1981 2255 3030 3278 1981
Size: 274 775 248 4303
Fragments arranged by size:
4303 775 274 248
KpnI G_GTAC'C
Cuts at: 524 2620 524
Size: 2096 3504
MluI A'CGCG_T
Cuts at: 5033 5033
Size: 5600
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1778 1898 4271 4455 1778
Size: 120 2373 184 2923
Fragments arranged by size:
2923 2373 184 120
MscI TGG'CCA
Cuts at: 1363 1516 2194 3361 1363
Size: 153 678 1167 3602
Fragments arranged by size:
3602 1167 678 153
MslI CAynn'nnrTG
Cuts at: 820 896 3589 3716 820
Size: 76 2693 127 2704
Fragments arranged by size:
2704 2693 127 76
MspA1I CmG'CkG
Cuts at: 243 651 732 2711 3025 3385 4398 4643 243
Size: 408 81 1979 314 360 1013 245 1200
Fragments arranged by size:
1979 1200 1013 408 360 314 245 81
NaeI GCC'GGC
Cuts at: 1845 3781 1845
Size: 1936 3664
NarI GG'CG_CC
Cuts at: 1982 2256 3031 3279 1982
Size: 274 775 248 4303
Fragments arranged by size:
4303 775 274 248
NcoI C'CATG_G
Cuts at: 107 1041 1364 2151 107
Size: 934 323 787 3556
Fragments arranged by size:
3556 934 787 323
NgoMIV G'CCGG_C
Cuts at: 1843 3779 1843
Size: 1936 3664
NspI r_CATG'y
Cuts at: 421 645 1636 2421 2448 2653 3153 4060 421
Size: 224 991 785 27 205 500 907 1961
Fragments arranged by size:
1961 991 907 785 500 224 205 27
PciI A'CATG_T
Cuts at: 417 641 4056 417
Size: 224 3415 1961
Fragments arranged by size:
3415 1961 224
PflMI CCAn_nnn'nTGG
Cuts at: 331 714 735 1627 1741 331
Size: 383 21 892 114 4190
Fragments arranged by size:
4190 892 383 114 21
PmeI GTTT'AAAC
Cuts at: 2599 2599
Size: 5600
PmlI CAC'GTG
Cuts at: 453 1077 1403 2320 453
Size: 624 326 917 3733
Fragments arranged by size:
3733 917 624 326
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4739 4771 4739
Size: 32 5568
PpuMI rG'GwC_Cy
Cuts at: 722 940 2181 2550 722
Size: 218 1241 369 3772
Fragments arranged by size:
3772 1241 369 218
PspOMI G'GGCC_C
Cuts at: 306 353 306
Size: 47 5553
PspXI vC'TCGA_Gb
Cuts at: 1543 1543
Size: 5600
PstI C_TGCA'G
Cuts at: 177 1979 2647 3332 177
Size: 1802 668 685 2445
Fragments arranged by size:
2445 1802 685 668
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5600
PvuII CAG'CTG
Cuts at: 651 732 3025 3385 651
Size: 81 2293 360 2866
Fragments arranged by size:
2866 2293 360 81
RsrII CG'GwC_CG
Cuts at: 3795 3795
Size: 5600
SacI G_AGCT'C
Cuts at: 507 1151 2614 507
Size: 644 1463 3493
Fragments arranged by size:
3493 1463 644
SalI G'TCGA_C
Cuts at: 2637 2637
Size: 5600
SanDI GG'GwC_CC
Cuts at: 940 940
Size: 5600
SapI GCTCTTCn'nnn_
Cuts at: 3623 3833 3623
Size: 210 5390
SbfI CC_TGCA'GG
Cuts at: 1979 2647 1979
Size: 668 4932
ScaI AGT'ACT
Cuts at: 5117 5117
Size: 5600
SexAI A'CCwGG_T
Cuts at: 470 1158 470
Size: 688 4912
SfcI C'TryA_G
Cuts at: 33 173 388 498 1975 2211 2643 3328 4321
Size: 140 215 110 1477 236 432 685 993
Cuts at: 4321 4512 33
Size: 191 1121
Fragments arranged by size:
1477 1121 993 685 432 236 215 191
140 110
SfoI GGC'GCC
Cuts at: 1983 2257 3032 3280 1983
Size: 274 775 248 4303
Fragments arranged by size:
4303 775 274 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5600
SmaI CCC'GGG
Cuts at: 938 1503 2622 938
Size: 565 1119 3916
Fragments arranged by size:
3916 1119 565
SmlI C'TyrA_G
Cuts at: 488 550 1543 2755 3670 4162 4424 488
Size: 62 993 1212 915 492 262 1664
Fragments arranged by size:
1664 1212 993 915 492 262 62
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5600
SphI G_CATG'C
Cuts at: 2421 2448 2653 3153 2421
Size: 27 205 500 4868
Fragments arranged by size:
4868 500 205 27
StuI AGG'CCT
Cuts at: 528 766 528
Size: 238 5362
StyI C'CwwG_G
Cuts at: 107 161 686 1041 1364 2151 2223 2294 2734
Size: 54 525 355 323 787 72 71 440
Cuts at: 2734 3088 107
Size: 354 2619
Fragments arranged by size:
2619 787 525 440 355 354 323 72
71 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 377 745 810 2298 2539 3183 3316 3775 3984
Size: 368 65 1488 241 644 133 459 209
Cuts at: 3984 377
Size: 1993
Fragments arranged by size:
1993 1488 644 459 368 241 209 133
65
TatI w'GTAC_w
Cuts at: 5115 5115
Size: 5600
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 888 920 2211 2243 2398 2430 5117 5149 888
Size: 32 1291 32 155 32 2687 32 1339
Fragments arranged by size:
2687 1339 1291 155 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2535 3397 2535
Size: 862 4738
XbaI T'CTAG_A
Cuts at: 2631 2631
Size: 5600
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 789 2269 3095 789
Size: 1480 826 3294
Fragments arranged by size:
3294 1480 826
XhoI C'TCGA_G
Cuts at: 1543 1543
Size: 5600
XmaI C'CCGG_G
Cuts at: 936 1501 2620 936
Size: 565 1119 3916
Fragments arranged by size:
3916 1119 565
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BbsI
BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstZ17I ClaI CspCI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PshAI PsiI PsrI SacII SfiI SgrAI SnaBI SrfI
SspI SwaI XmnI ZraI