Restriction map for
pF1KB5791
(Circular) (Six-base) MAPSORT of: pF1KB5791.seq Check: 7914 from: 1 to: 5603
pF1KB5791 5603 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 661 1546 1906 661
Size: 885 360 4358
Fragments arranged by size:
4358 885 360
AccI GT'mk_AC
Cuts at: 2641 2641
Size: 5603
Acc65I G'GTAC_C
Cuts at: 806 1514 2619 806
Size: 708 1105 3790
Fragments arranged by size:
3790 1105 708
AclI AA'CG_TT
Cuts at: 5443 5443
Size: 5603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 922 960 1379 2156 2262 3428 3860 4607
Size: 754 38 419 777 106 1166 432 747
Cuts at: 4607 4875 168
Size: 268 896
Fragments arranged by size:
1166 896 777 754 747 432 419 268
106 38
AflIII A'CryG_T
Cuts at: 602 729 1711 2229 2955 4059 5036 602
Size: 127 982 518 726 1104 977 1169
Fragments arranged by size:
1169 1104 982 977 726 518 127
AgeI A'CCGG_T
Cuts at: 3991 3991
Size: 5603
AleI CACnn'nnGTG
Cuts at: 1803 1803
Size: 5603
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1660 1660
Size: 5603
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 704 736 704
Size: 32 5571
AlwNI CAG_nnn'CTG
Cuts at: 532 1086 2908 4475 532
Size: 554 1822 1567 1660
Fragments arranged by size:
1822 1660 1567 554
ApaI G_GGCC'C
Cuts at: 824 1940 824
Size: 1116 4487
ApaLI G'TGCA_C
Cuts at: 4373 4373
Size: 5603
ApoI r'AATT_y
Cuts at: 164 1159 1602 1879 2607 4956 5212 164
Size: 995 443 277 728 2349 256 555
Fragments arranged by size:
2349 995 728 555 443 277 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5603
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5603
AvaI C'yCGr_G
Cuts at: 631 1032 1478 2589 2623 631
Size: 401 446 1111 34 3611
Fragments arranged by size:
3611 1111 446 401 34
AvrII C'CTAG_G
Cuts at: 2550 2550
Size: 5603
BamHI G'GATC_C
Cuts at: 386 2628 386
Size: 2242 3361
BanI G'GyrC_C
Cuts at: 806 1504 1514 2619 3033 3281 3316 806
Size: 698 10 1105 414 248 35 3093
Fragments arranged by size:
3093 1105 698 414 248 35 10
BanII G_rGCy'C
Cuts at: 722 824 1940 2617 3647 722
Size: 102 1116 677 1030 2678
Fragments arranged by size:
2678 1116 1030 677 102
BbeI G_GCGC'C
Cuts at: 3037 3285 3037
Size: 248 5355
BciVI GTATCCnnnnn_n'
Cuts at: 3501 4262 5603 3501
Size: 761 1341 3501
Fragments arranged by size:
3501 1341 761
BclI T'GATC_A
Cuts at: 3123 3123
Size: 5603
BglI GCCn_nnn'nGGC
Cuts at: 1175 1175
Size: 5603
BglII A'GATC_T
Cuts at: 3118 5155 3118
Size: 2037 3566
BlpI GC'TnA_GC
Cuts at: 1648 2715 1648
Size: 1067 4536
Bme1580I G_kGCm'C
Cuts at: 192 824 895 1825 1940 3228 3321 4377 192
Size: 632 71 930 115 1288 93 1056 1418
Fragments arranged by size:
1418 1288 1056 930 632 115 93 71
BmrI ACTGGGnnnn_n'
Cuts at: 465 882 1051 1423 1456 2815 2896 3231 5375
Size: 417 169 372 33 1359 81 335 2144
Cuts at: 5375 465
Size: 693
Fragments arranged by size:
2144 1359 693 417 372 335 169 81
33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 646 3970 646
Size: 3324 2279
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1134 1294 1598 1951 2003 2180 1134
Size: 160 304 353 52 177 4557
Fragments arranged by size:
4557 353 304 177 160 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 736 1335 2779 3658 4150 4448 736
Size: 599 1444 879 492 298 1891
Fragments arranged by size:
1891 1444 879 599 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 628 5307 628
Size: 4679 924
BsaAI yAC'GTr
Cuts at: 696 732 2956 696
Size: 36 2224 3343
Fragments arranged by size:
3343 2224 36
BsaBI GATnn'nnATC
Cuts at: 6 385 511 3117 5154 6
Size: 379 126 2606 2037 455
Fragments arranged by size:
2606 2037 455 379 126
BsaHI Gr'CG_yC
Cuts at: 3034 3282 3984 5480 3034
Size: 248 702 1496 3157
Fragments arranged by size:
3157 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1008 2792 3313 3991 4265 4412 4823 1008
Size: 1784 521 678 274 147 411 1788
Fragments arranged by size:
1788 1784 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 352 382 574 604 640 670 704 734 1556
Size: 30 192 30 36 30 34 30 822
Cuts at: 1556 1586 352
Size: 30 4369
Fragments arranged by size:
4369 822 192 36 34 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1391 1391
Size: 5603
BseYI C'CCAG_C
Cuts at: 823 1050 3028 3786 4363 4859 823
Size: 227 1978 758 577 496 1567
Fragments arranged by size:
1978 1567 758 577 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 780 804 1093 1217 1620 2321 780
Size: 24 289 124 403 701 4062
Fragments arranged by size:
4062 701 403 289 124 24
BsiEI CG_ry'CG
Cuts at: 104 1777 3145 3191 4399 104
Size: 1673 1368 46 1208 1308
Fragments arranged by size:
1673 1368 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 554 722 737 2617 3395 3585 4377 554
Size: 168 15 1880 778 190 792 1780
Fragments arranged by size:
1880 1780 792 778 190 168 15
BsmI GAATG_Cn'
Cuts at: 841 949 1624 1957 841
Size: 108 675 333 4487
Fragments arranged by size:
4487 675 333 108
Bsp1286I G_dGCh'C
Cuts at: 192 554 722 737 824 895 1825 1940 2617
Size: 362 168 15 87 71 930 115 677
Cuts at: 2617 3228 3321 3395 3585 3647 4377 192
Size: 611 93 74 190 62 730 1418
Fragments arranged by size:
1418 930 730 677 611 362 190 168
115 93 87 74 71 62 15
BspEI T'CCGG_A
Cuts at: 4823 4823
Size: 5603
BspHI T'CATG_A
Cuts at: 737 4779 737
Size: 4042 1561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 661 937 1546 1906 1999 2395 2653 2867 3169
Size: 276 609 360 93 396 258 214 302
Cuts at: 3169 3550 661
Size: 381 2714
Fragments arranged by size:
2714 609 396 381 360 302 276 258
214 93
BsrBI CCG'CTC
Cuts at: 50 1128 2440 3895 3949 5433 5598 50
Size: 1078 1312 1455 54 1484 165 55
Fragments arranged by size:
1484 1455 1312 1078 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1027 1548 2035 3515 1027
Size: 521 487 1480 3115
Fragments arranged by size:
3115 1480 521 487
BsrFI r'CCGG_y
Cuts at: 3601 3782 3991 3601
Size: 181 209 5213
Fragments arranged by size:
5213 209 181
BssSI C'ACGA_G
Cuts at: 1871 3874 4232 1871
Size: 2003 358 3242
Fragments arranged by size:
3242 2003 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1301 2980 1301
Size: 1679 3924
BstBI TT'CG_AA
Cuts at: 3964 3964
Size: 5603
BstXI CCAn_nnnn'nTGG
Cuts at: 817 831 1947 2359 817
Size: 14 1116 412 4061
Fragments arranged by size:
4061 1116 412 14
BstYI r'GATC_y
Cuts at: 1 386 1455 2628 3095 3118 3453 3699 4711
Size: 385 1069 1173 467 23 335 246 1012
Cuts at: 4711 4797 4809 5155 1
Size: 86 12 346 449
Fragments arranged by size:
1173 1069 1012 467 449 385 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1109 1287 107
Size: 1002 178 4423
Fragments arranged by size:
4423 1002 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2263 3553 3734 3897 2263
Size: 1290 181 163 3969
Fragments arranged by size:
3969 1290 181 163
BtsI GCAGTG_nn'
Cuts at: 552 787 792 1081 1130 1453 2846 552
Size: 235 5 289 49 323 1393 3309
Fragments arranged by size:
3309 1393 323 289 235 49 5
DraI TTT'AAA
Cuts at: 2602 2602
Size: 5603
DrdI GACnn_nn'nnGTC
Cuts at: 3309 4167 3309
Size: 858 4745
EaeI y'GGCC_r
Cuts at: 110 311 2361 3188 3362 3753 3780 5125 110
Size: 201 2050 827 174 391 27 1345 588
Fragments arranged by size:
2050 1345 827 588 391 201 174 27
EagI C'GGCC_G
Cuts at: 3188 3188
Size: 5603
EarI CTCTTCn'nnn_
Cuts at: 980 3626 3836 980
Size: 2646 210 2747
Fragments arranged by size:
2747 2646 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3811 4121 4267 5413 3811
Size: 310 146 1146 4001
Fragments arranged by size:
4001 1146 310 146
EcoICRI GAG'CTC
Cuts at: 720 2615 720
Size: 1895 3708
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 922 960 1134 1294 1379 1598 1951 2003
Size: 754 38 174 160 85 219 353 52
Cuts at: 2003 2156 2180 2262 3428 3860 4607 4875 168
Size: 153 24 82 1166 432 747 268 896
Fragments arranged by size:
1166 896 754 747 432 353 268 219
174 160 153 85 82 52 38 24
EcoNI CCTnn'n_nnAGG
Cuts at: 346 914 2018 346
Size: 568 1104 3931
Fragments arranged by size:
3931 1104 568
EcoO109I rG'GnC_Cy
Cuts at: 351 1936 2742 351
Size: 1585 806 3212
Fragments arranged by size:
3212 1585 806
EcoRI G'AATT_C
Cuts at: 1602 1879 2607 1602
Size: 277 728 4598
Fragments arranged by size:
4598 728 277
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3622 3622
Size: 5603
FspI TGC'GCA
Cuts at: 3384 3384
Size: 5603
HaeII r_GCGC'y
Cuts at: 3037 3285 4307 5286 3037
Size: 248 1022 979 3354
Fragments arranged by size:
3354 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 3445 3475 3477 3507 2123
Size: 32 1290 30 2 30 4219
Fragments arranged by size:
4219 1290 32 30 30 2
HincII GTy'rAC
Cuts at: 2372 2642 2372
Size: 270 5333
HindIII A'AGCT_T
Cuts at: 1439 1439
Size: 5603
KasI G'GCGC_C
Cuts at: 3033 3281 3033
Size: 248 5355
KpnI G_GTAC'C
Cuts at: 810 1518 2623 810
Size: 708 1105 3790
Fragments arranged by size:
3790 1105 708
MluI A'CGCG_T
Cuts at: 5036 5036
Size: 5603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 984 2285 4274 4458 984
Size: 1301 1989 184 2129
Fragments arranged by size:
2129 1989 1301 184
MscI TGG'CCA
Cuts at: 112 313 2363 3364 112
Size: 201 2050 1001 2351
Fragments arranged by size:
2351 2050 1001 201
MslI CAynn'nnrTG
Cuts at: 261 411 1803 3592 3719 261
Size: 150 1392 1789 127 2145
Fragments arranged by size:
2145 1789 1392 150 127
MspA1I CmG'CkG
Cuts at: 1154 1208 1272 1352 2162 2714 3028 3388 4401
Size: 54 64 80 810 552 314 360 1013
Cuts at: 4401 4646 1154
Size: 245 2111
Fragments arranged by size:
2111 1013 810 552 360 314 245 80
64 54
NaeI GCC'GGC
Cuts at: 3784 3784
Size: 5603
NarI GG'CG_CC
Cuts at: 3034 3282 3034
Size: 248 5355
NcoI C'CATG_G
Cuts at: 107 1109 107
Size: 1002 4601
NdeI CA'TA_TG
Cuts at: 2559 2559
Size: 5603
NgoMIV G'CCGG_C
Cuts at: 3782 3782
Size: 5603
NspI r_CATG'y
Cuts at: 606 1203 1715 2204 2233 2351 2656 3156 4063
Size: 597 512 489 29 118 305 500 907
Cuts at: 4063 606
Size: 2146
Fragments arranged by size:
2146 907 597 512 500 489 305 118
29
PciI A'CATG_T
Cuts at: 602 1711 2229 4059 602
Size: 1109 518 1830 2146
Fragments arranged by size:
2146 1830 1109 518
PflMI CCAn_nnn'nTGG
Cuts at: 153 756 2267 153
Size: 603 1511 3489
Fragments arranged by size:
3489 1511 603
PfoI T'CCnGG_A
Cuts at: 2082 2411 2082
Size: 329 5274
PmeI GTTT'AAAC
Cuts at: 2602 2602
Size: 5603
PmlI CAC'GTG
Cuts at: 732 732
Size: 5603
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 704 736 4742 4774 704
Size: 32 4006 32 1533
Fragments arranged by size:
4006 1533 32 32
PpuMI rG'GwC_Cy
Cuts at: 351 351
Size: 5603
PshAI GACnn'nnGTC
Cuts at: 1227 2591 1227
Size: 1364 4239
PsiI TTA'TAA
Cuts at: 1703 1703
Size: 5603
PspOMI G'GGCC_C
Cuts at: 820 1936 820
Size: 1116 4487
PstI C_TGCA'G
Cuts at: 613 748 973 1531 2167 2650 3335 613
Size: 135 225 558 636 483 685 2881
Fragments arranged by size:
2881 685 636 558 483 225 135
PvuI CG_AT'CG
Cuts at: 104 1777 104
Size: 1673 3930
PvuII CAG'CTG
Cuts at: 1154 2162 3028 3388 1154
Size: 1008 866 360 3369
Fragments arranged by size:
3369 1008 866 360
RsrII CG'GwC_CG
Cuts at: 3798 3798
Size: 5603
SacI G_AGCT'C
Cuts at: 722 2617 722
Size: 1895 3708
SalI G'TCGA_C
Cuts at: 2640 2640
Size: 5603
SapI GCTCTTCn'nnn_
Cuts at: 3626 3836 3626
Size: 210 5393
SbfI CC_TGCA'GG
Cuts at: 2650 2650
Size: 5603
ScaI AGT'ACT
Cuts at: 5120 5120
Size: 5603
SfcI C'TryA_G
Cuts at: 33 363 609 744 969 1179 1341 1527 1576
Size: 330 246 135 225 210 162 186 49
Cuts at: 1576 2070 2163 2646 3331 4324 4515 33
Size: 494 93 483 685 993 191 1121
Fragments arranged by size:
1121 993 685 494 483 330 246 225
210 191 186 162 135 93 49
SfoI GGC'GCC
Cuts at: 3035 3283 3035
Size: 248 5355
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5603
SmaI CCC'GGG
Cuts at: 1480 2625 1480
Size: 1145 4458
SmlI C'TyrA_G
Cuts at: 631 715 1314 2758 3673 4165 4427 631
Size: 84 599 1444 915 492 262 1807
Fragments arranged by size:
1807 1444 915 599 492 262 84
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5603
SphI G_CATG'C
Cuts at: 2204 2351 2656 3156 2204
Size: 147 305 500 4651
Fragments arranged by size:
4651 500 305 147
SspI AAT'ATT
Cuts at: 270 270
Size: 5603
StyI C'CwwG_G
Cuts at: 107 1109 2550 2737 3091 107
Size: 1002 1441 187 354 2619
Fragments arranged by size:
2619 1441 1002 354 187
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 405 2458 3186 3319 3778 3987 405
Size: 2053 728 133 459 209 2021
Fragments arranged by size:
2053 2021 728 459 209 133
TatI w'GTAC_w
Cuts at: 1768 2066 5118 1768
Size: 298 3052 2253
Fragments arranged by size:
3052 2253 298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 998 1030 1556 1588 5120 5152 998
Size: 32 526 32 3532 32 1449
Fragments arranged by size:
3532 1449 526 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3400 3400
Size: 5603
XbaI T'CTAG_A
Cuts at: 2310 2634 2310
Size: 324 5279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2326 3098 2326
Size: 772 4831
XhoI C'TCGA_G
Cuts at: 631 631
Size: 5603
XmaI C'CCGG_G
Cuts at: 1478 2623 1478
Size: 1145 4458
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NspI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AscI BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HpaI MfeI NheI NotI NruI NsiI
PacI PasI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI ZraI