Restriction map for
pF1KB5796
(Circular) (Six-base) MAPSORT of: pF1KB5796.seq Check: 2867 from: 1 to: 5453
pF1KB5796 5453 bp
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AccI GT'mk_AC
Cuts at: 2491 2491
Size: 5453
Acc65I G'GTAC_C
Cuts at: 2469 2469
Size: 5453
AclI AA'CG_TT
Cuts at: 5293 5293
Size: 5453
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1538 2287 2424 3278 3710 4457 4725 1538
Size: 749 137 854 432 747 268 2266
Fragments arranged by size:
2266 854 749 747 432 268 137
AfeI AGC'GCT
Cuts at: 1942 1942
Size: 5453
AflIII A'CryG_T
Cuts at: 1224 2805 3909 4886 1224
Size: 1581 1104 977 1791
Fragments arranged by size:
1791 1581 1104 977
AgeI A'CCGG_T
Cuts at: 3841 3841
Size: 5453
AleI CACnn'nnGTG
Cuts at: 266 614 632 914 929 266
Size: 348 18 282 15 4790
Fragments arranged by size:
4790 348 282 18 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2308 2340 2308
Size: 32 5421
AlwNI CAG_nnn'CTG
Cuts at: 127 1380 2758 4325 127
Size: 1253 1378 1567 1255
Fragments arranged by size:
1567 1378 1255 1253
ApaI G_GGCC'C
Cuts at: 1767 1767
Size: 5453
ApaLI G'TGCA_C
Cuts at: 4223 4223
Size: 5453
ApoI r'AATT_y
Cuts at: 579 2457 4806 5062 579
Size: 1878 2349 256 970
Fragments arranged by size:
2349 1878 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5453
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
AvaI C'yCGr_G
Cuts at: 2473 2473
Size: 5453
BamHI G'GATC_C
Cuts at: 1736 2183 2478 1736
Size: 447 295 4711
Fragments arranged by size:
4711 447 295
BanI G'GyrC_C
Cuts at: 415 992 2035 2257 2469 2883 3131 3166 415
Size: 577 1043 222 212 414 248 35 2702
Fragments arranged by size:
2702 1043 577 414 248 222 212 35
BanII G_rGCy'C
Cuts at: 225 378 464 713 1183 1767 2222 2237 2321
Size: 153 86 249 470 584 455 15 84
Cuts at: 2321 2467 3497 225
Size: 146 1030 2181
Fragments arranged by size:
2181 1030 584 470 455 249 153 146
86 84 15
BbeI G_GCGC'C
Cuts at: 419 2039 2887 3135 419
Size: 1620 848 248 2737
Fragments arranged by size:
2737 1620 848 248
BbsI GAAGACnn'nnnn_
Cuts at: 1143 1289 1740 2210 1143
Size: 146 451 470 4386
Fragments arranged by size:
4386 470 451 146
BciVI GTATCCnnnnn_n'
Cuts at: 3351 4112 5453 3351
Size: 761 1341 3351
Fragments arranged by size:
3351 1341 761
BclI T'GATC_A
Cuts at: 918 2973 918
Size: 2055 3398
BglI GCCn_nnn'nGGC
Cuts at: 389 1771 389
Size: 1382 4071
BglII A'GATC_T
Cuts at: 2968 5005 2968
Size: 2037 3416
BlpI GC'TnA_GC
Cuts at: 1795 2565 1795
Size: 770 4683
Bme1580I G_kGCm'C
Cuts at: 260 608 1767 1917 2031 3078 3171 4227 260
Size: 348 1159 150 114 1047 93 1056 1486
Fragments arranged by size:
1486 1159 1056 1047 348 150 114 93
BmrI ACTGGGnnnn_n'
Cuts at: 923 1296 2665 2746 3081 5225 923
Size: 373 1369 81 335 2144 1151
Fragments arranged by size:
2144 1369 1151 373 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3820 3820
Size: 5453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1191 1318 2447 1191
Size: 127 1129 4197
Fragments arranged by size:
4197 1129 127
Bpu10I CC'TnA_GC
Cuts at: 703 703
Size: 5453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 395 1582 1726 2173 2629 3508 4000 4298 395
Size: 1187 144 447 456 879 492 298 1550
Fragments arranged by size:
1550 1187 879 492 456 447 298 144
BsaI GGTCTCn'nnnn_
Cuts at: 551 835 5157 551
Size: 284 4322 847
Fragments arranged by size:
4322 847 284
BsaAI yAC'GTr
Cuts at: 1164 1225 1581 2806 1164
Size: 61 356 1225 3811
Fragments arranged by size:
3811 1225 356 61
BsaBI GATnn'nnATC
Cuts at: 6 2967 5004 6
Size: 2961 2037 455
Fragments arranged by size:
2961 2037 455
BsaHI Gr'CG_yC
Cuts at: 416 2036 2884 3132 3834 5330 416
Size: 1620 848 248 702 1496 539
Fragments arranged by size:
1620 1496 848 702 539 248
BsaWI w'CCGG_w
Cuts at: 288 1427 1733 2642 3163 3841 4115 4262 4673
Size: 1139 306 909 521 678 274 147 411
Cuts at: 4673 288
Size: 1068
Fragments arranged by size:
1139 1068 909 678 521 411 306 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 2308 2338 2308
Size: 30 5423
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 818 818
Size: 5453
BseYI C'CCAG_C
Cuts at: 728 2878 3636 4213 4709 728
Size: 2150 758 577 496 1472
Fragments arranged by size:
2150 1472 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1329 2995 3041 4249 104
Size: 1225 1666 46 1208 1308
Fragments arranged by size:
1666 1308 1225 1208 46
BsiHKAI G_wGCw'C
Cuts at: 225 464 1072 2222 2321 2467 3245 3435 4227
Size: 239 608 1150 99 146 778 190 792
Cuts at: 4227 225
Size: 1451
Fragments arranged by size:
1451 1150 792 778 608 239 190 146
99
Bsp1286I G_dGCh'C
Cuts at: 225 260 378 464 608 713 1072 1183 1767
Size: 35 118 86 144 105 359 111 584
Cuts at: 1767 1917 2031 2222 2237 2321 2467 3078 3171
Size: 150 114 191 15 84 146 611 93
Cuts at: 3171 3245 3435 3497 4227 225
Size: 74 190 62 730 1451
Fragments arranged by size:
1451 730 611 584 359 191 190 150
146 144 118 114 111 105 93 86
84 74 62 35 15
BspEI T'CCGG_A
Cuts at: 288 1427 1733 4673 288
Size: 1139 306 2940 1068
Fragments arranged by size:
2940 1139 1068 306
BspHI T'CATG_A
Cuts at: 486 801 4629 486
Size: 315 3828 1310
Fragments arranged by size:
3828 1310 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 779 1891 2342 2503 2717 3019 3400 779
Size: 1112 451 161 214 302 381 2832
Fragments arranged by size:
2832 1112 451 381 302 214 161
BsrBI CCG'CTC
Cuts at: 50 1220 2378 3745 3799 5283 5448 50
Size: 1170 1158 1367 54 1484 165 55
Fragments arranged by size:
1484 1367 1170 1158 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3365 3365
Size: 5453
BsrFI r'CCGG_y
Cuts at: 989 1324 1328 3451 3632 3841 989
Size: 335 4 2123 181 209 2601
Fragments arranged by size:
2601 2123 335 209 181 4
BssSI C'ACGA_G
Cuts at: 3724 4082 3724
Size: 358 5095
BstAPI GCAn_nnn'nTGC
Cuts at: 2830 2830
Size: 5453
BstBI TT'CG_AA
Cuts at: 3814 3814
Size: 5453
BstXI CCAn_nnnn'nTGG
Cuts at: 268 472 505 634 784 817 2244 268
Size: 204 33 129 150 33 1427 3477
Fragments arranged by size:
3477 1427 204 150 129 33 33
BstYI r'GATC_y
Cuts at: 1 1526 1736 2183 2478 2945 2968 3303 3549
Size: 1525 210 447 295 467 23 335 246
Cuts at: 3549 4561 4647 4659 5005 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1525 1012 467 449 447 346 335 295
246 210 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1768 1768
Size: 5453
BtgI C'CryG_G
Cuts at: 107 378 552 612 630 651 750 783 942
Size: 271 174 60 18 21 99 33 159
Cuts at: 942 1023 1050 1174 1667 1901 107
Size: 81 27 124 493 234 3659
Fragments arranged by size:
3659 493 271 234 174 159 124 99
81 60 33 27 21 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1590 1839 3403 3584 3747 1590
Size: 249 1564 181 163 3296
Fragments arranged by size:
3296 1564 249 181 163
BtsI GCAGTG_nn'
Cuts at: 2696 2696
Size: 5453
ClaI AT'CG_AT
Cuts at: 2396 2396
Size: 5453
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 375 410 375
Size: 35 5418
DraI TTT'AAA
Cuts at: 2452 2452
Size: 5453
DraIII CAC_nnn'GTG
Cuts at: 255 255
Size: 5453
DrdI GACnn_nn'nnGTC
Cuts at: 3159 4017 3159
Size: 858 4595
EaeI y'GGCC_r
Cuts at: 1326 1664 1670 3038 3212 3603 3630 4975 1326
Size: 338 6 1368 174 391 27 1345 1804
Fragments arranged by size:
1804 1368 1345 391 338 174 27 6
EagI C'GGCC_G
Cuts at: 1326 3038 1326
Size: 1712 3741
EarI CTCTTCn'nnn_
Cuts at: 1375 1735 1894 1900 1981 2426 3476 3686 1375
Size: 360 159 6 81 445 1050 210 3142
Fragments arranged by size:
3142 1050 445 360 210 159 81 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3661 3971 4117 5263 3661
Size: 310 146 1146 3851
Fragments arranged by size:
3851 1146 310 146
EcoICRI GAG'CTC
Cuts at: 223 462 2220 2319 2465 223
Size: 239 1758 99 146 3211
Fragments arranged by size:
3211 1758 239 146 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1191 1318 1538 2287 2424 2447 3278 3710 4457
Size: 127 220 749 137 23 831 432 747
Cuts at: 4457 4725 1191
Size: 268 1919
Fragments arranged by size:
1919 831 749 747 432 268 220 137
127 23
EcoNI CCTnn'n_nnAGG
Cuts at: 1405 1405
Size: 5453
EcoO109I rG'GnC_Cy
Cuts at: 494 638 791 869 1764 1782 1829 2592 494
Size: 144 153 78 895 18 47 763 3355
Fragments arranged by size:
3355 895 763 153 144 78 47 18
EcoRI G'AATT_C
Cuts at: 2457 2457
Size: 5453
EcoRV GAT'ATC
Cuts at: 887 1263 887
Size: 376 5077
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1413 3472 1413
Size: 2059 3394
FspI TGC'GCA
Cuts at: 3234 3234
Size: 5453
HaeII r_GCGC'y
Cuts at: 419 1944 2039 2140 2887 3135 4157 5136 419
Size: 1525 95 101 747 248 1022 979 736
Fragments arranged by size:
1525 1022 979 747 736 248 101 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 103 135 568 600 1987 2019 3295 3325 3327
Size: 32 433 32 1387 32 1276 30 2
Cuts at: 3327 3357 103
Size: 30 2199
Fragments arranged by size:
2199 1387 1276 433 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2492 2492
Size: 5453
KasI G'GCGC_C
Cuts at: 415 2035 2883 3131 415
Size: 1620 848 248 2737
Fragments arranged by size:
2737 1620 848 248
KpnI G_GTAC'C
Cuts at: 2473 2473
Size: 5453
MluI A'CGCG_T
Cuts at: 4886 4886
Size: 5453
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 489 738 4124 4308 489
Size: 249 3386 184 1634
Fragments arranged by size:
3386 1634 249 184
MscI TGG'CCA
Cuts at: 1666 3214 1666
Size: 1548 3905
MslI CAynn'nnrTG
Cuts at: 266 488 614 632 782 815 914 929 1234
Size: 222 126 18 150 33 99 15 305
Cuts at: 1234 1492 1498 1579 1672 1873 2014 3442 3569
Size: 258 6 81 93 201 141 1428 127
Cuts at: 3569 266
Size: 2150
Fragments arranged by size:
2150 1428 305 258 222 201 150 141
127 126 99 93 81 33 18 15
6
MspA1I CmG'CkG
Cuts at: 1338 1903 2340 2564 2878 3238 4251 4496 1338
Size: 565 437 224 314 360 1013 245 2295
Fragments arranged by size:
2295 1013 565 437 360 314 245 224
NaeI GCC'GGC
Cuts at: 1330 3634 1330
Size: 2304 3149
NarI GG'CG_CC
Cuts at: 416 2036 2884 3132 416
Size: 1620 848 248 2737
Fragments arranged by size:
2737 1620 848 248
NcoI C'CATG_G
Cuts at: 107 378 552 630 651 783 942 1050 1667
Size: 271 174 78 21 132 159 108 617
Cuts at: 1667 107
Size: 3893
Fragments arranged by size:
3893 617 271 174 159 132 108 78
21
NgoMIV G'CCGG_C
Cuts at: 1328 3632 1328
Size: 2304 3149
NspI r_CATG'y
Cuts at: 2506 3006 3913 2506
Size: 500 907 4046
Fragments arranged by size:
4046 907 500
PasI CC'CwG_GG
Cuts at: 1611 1833 2031 1611
Size: 222 198 5033
Fragments arranged by size:
5033 222 198
PciI A'CATG_T
Cuts at: 3909 3909
Size: 5453
PflMI CCAn_nnn'nTGG
Cuts at: 1446 1446
Size: 5453
PfoI T'CCnGG_A
Cuts at: 1099 1415 1814 1099
Size: 316 399 4738
Fragments arranged by size:
4738 399 316
PmeI GTTT'AAAC
Cuts at: 2452 2452
Size: 5453
PmlI CAC'GTG
Cuts at: 1225 1225
Size: 5453
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2308 2340 4592 4624 2308
Size: 32 2252 32 3137
Fragments arranged by size:
3137 2252 32 32
PpuMI rG'GwC_Cy
Cuts at: 494 638 791 869 1782 1829 494
Size: 144 153 78 913 47 4118
Fragments arranged by size:
4118 913 153 144 78 47
PshAI GACnn'nnGTC
Cuts at: 1810 1810
Size: 5453
PspOMI G'GGCC_C
Cuts at: 1763 1763
Size: 5453
PstI C_TGCA'G
Cuts at: 2232 2339 2500 3185 2232
Size: 107 161 685 4500
Fragments arranged by size:
4500 685 161 107
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5453
PvuII CAG'CTG
Cuts at: 2340 2878 3238 2340
Size: 538 360 4555
Fragments arranged by size:
4555 538 360
RsrII CG'GwC_CG
Cuts at: 3648 3648
Size: 5453
SacI G_AGCT'C
Cuts at: 225 464 2222 2321 2467 225
Size: 239 1758 99 146 3211
Fragments arranged by size:
3211 1758 239 146 99
SacII CC_GC'GG
Cuts at: 1904 1904
Size: 5453
SalI G'TCGA_C
Cuts at: 2490 2490
Size: 5453
SanDI GG'GwC_CC
Cuts at: 494 1829 494
Size: 1335 4118
SapI GCTCTTCn'nnn_
Cuts at: 3476 3686 3476
Size: 210 5243
SbfI CC_TGCA'GG
Cuts at: 2500 2500
Size: 5453
ScaI AGT'ACT
Cuts at: 2083 4970 2083
Size: 2887 2566
SexAI A'CCwGG_T
Cuts at: 2099 2099
Size: 5453
SfcI C'TryA_G
Cuts at: 33 666 2228 2335 2496 3181 4174 4365 33
Size: 633 1562 107 161 685 993 191 1121
Fragments arranged by size:
1562 1121 993 685 633 191 161 107
SfoI GGC'GCC
Cuts at: 417 2037 2885 3133 417
Size: 1620 848 248 2737
Fragments arranged by size:
2737 1620 848 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5453
SmaI CCC'GGG
Cuts at: 2475 2475
Size: 5453
SmlI C'TyrA_G
Cuts at: 410 1597 1741 2152 2608 3523 4015 4277 410
Size: 1187 144 411 456 915 492 262 1586
Fragments arranged by size:
1586 1187 915 492 456 411 262 144
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5453
SphI G_CATG'C
Cuts at: 2506 3006 2506
Size: 500 4953
StuI AGG'CCT
Cuts at: 2150 2150
Size: 5453
StyI C'CwwG_G
Cuts at: 107 378 552 630 651 783 912 942 1050
Size: 271 174 78 21 132 129 30 108
Cuts at: 1050 1445 1553 1667 2587 2941 107
Size: 395 108 114 920 354 2619
Fragments arranged by size:
2619 920 395 354 271 174 132 129
114 108 108 78 30 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1187 1190 1246 3036 3169 3628 3837 1187
Size: 3 56 1790 133 459 209 2803
Fragments arranged by size:
2803 1790 459 209 133 56 3
TatI w'GTAC_w
Cuts at: 2081 4968 2081
Size: 2887 2566
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 709 741 4970 5002 709
Size: 32 4229 32 1160
Fragments arranged by size:
4229 1160 32 32
Tth111I GACn'n_nGTC
Cuts at: 1060 1200 3250 1060
Size: 140 2050 3263
Fragments arranged by size:
3263 2050 140
XbaI T'CTAG_A
Cuts at: 2484 2484
Size: 5453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 595 1674 2948 595
Size: 1079 1274 3100
Fragments arranged by size:
3100 1274 1079
XmaI C'CCGG_G
Cuts at: 2473 2473
Size: 5453
XmnI GAAnn'nnTTC
Cuts at: 1954 1954
Size: 5453
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AlfI AscI AvrII BaeI
BbvCI BcgI BfrBI BmgBI BmtI BsgI BsiWI BsmI
BsmBI BsrGI BssHII BstEII BstZ17I FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PsiI PspXI PsrI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI